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The map label for this gene is eutE [H]

Identifier: 153936261

GI number: 153936261

Start: 2176868

End: 2178466

Strand: Reverse

Name: eutE [H]

Synonym: CLC_2050

Alternate gene names: 153936261

Gene position: 2178466-2176868 (Counterclockwise)

Preceding gene: 153935738

Following gene: 153937619

Centisome position: 57.93

GC content: 34.96

Gene sequence:

>1599_bases
ATGGAAAACTTTGATAAGGATTTGCGTTCAATACAACAAGCGAGAGATCTTGCTAGACTTGGAAAAGTAGCAGCTGATAA
AATTGCTGATTATACTGAAGAGCAAATTGACAAAATTTTACGTAATATGGTAAGAGTTGCAGAAGAAAACGCTGTTTGTT
TAGCACAAATGGCAGTTGAAGAGACAGGCTTTGGTAAAGTTGAAGATAAAACTTTCAAAAACCACTTAGCAGCTACTATA
CTTTATAATTCAATTAAAGATATGAAAACTATAGGTGTAATTAATGAAGATAAGGAAAATAAGCTTATAGAACTTGCTGA
ACCAGTTGGCTTAGTTATGGGTATAGTACCTTCAACAAACCCAACTTCTACCGCAATTTTCAAAGCTATGATTTCAATCA
AATCTCGTAATGCTATAGTGTTCGCACCACATCCAGCTGCTGCAAAATGTACAATTAAAGCAGTTGAATTAATGAGGGAT
GCAGCAATAGAAGCTGGAGCACCAGAAAACATTATAAGTGCTGTAACTATTCCAACAATTGGAGCTACAAATGAATTAAT
GAAAAGCAAAGAAGTAGCTATAATAATAGCTACAGGTGGTCCAGGAATGGTTAAAGCTGCTTATAGCTCAGGAACACCAG
CTATTGGTGTTGGTGCAGGAAACTCTCCAGCATACATTGAAAGAACTGCTAATGTAGAAAAAGCTGTTAAAAACATTATT
GCAAGTAAAACTTTTGATAATGGTACAATCTGTGCATCAGAACAATCAATAATCTGTGAAGAATGTAATCATGACCTAGT
TGTTGCAGAATTTAAAAAACAAGGTGGATACTTCATGACAGAAGAAGAAACTGAAAAAGTTTGTAAACTATTATTCAAAA
AAGGTAATGCTATGAATGCTAAATTTGTTGGAAGAAGTCCACAAGTTATAGCAAGTGCTGCAGGATTCACAATTCCAGCA
GGAACTAAAGTACTTATAGGAAGACAAAATGGAGTAGGAGAAGGTTACCCATTATCTTTTGAAAAACTTACAACAGTTCT
TGGATTCTATACAGTAAAAGATTGGCATGAAGCATGTGAATTAAGTATTAAATTACTTCAAAATGGAATTGGTCACACAA
TTAGCATCCATACAGAAGATAGAGACATGGTTATGAAGTTTGCTAGAAAACCAGCTTCCCGTATCCTTGTTAACACTGGT
GGAACTCAAGGTGGAACAGGTGCAAGTACAGGTCTTAATCCTTCATTTACTTTAGGATGCGGTACTTGGGGAGGAAGCTC
AGTATCTGAAAATGTTACTCCAAAGCACCTTATGAACATTAAAAGAGTTGCTTATGGTATAAAAGATTGCACAAATTTAG
CATCTAATGACCCAACTTTTAACTGCATAAAAACTGCTGAAAACTGTCATGGAGTTCAAAATCAATTTATGAATATGAGC
CCAGCACAAATTGCAGCAGCTGCAGAAGTTTTAAATAAGGCTAATAATTGCGCTGAAAACACTAATACTTGCACTGAATG
CAGTAATGAAAATACAAAAAATGAAGAACTTTTAGATTTAGTTAATCAAATAGTTGCCGCTATGAAGGGGGCAAACTAA

Upstream 100 bases:

>100_bases
ACAATTTCAAAAGCAGGTAAAAATTTATTAATTAACAAATTTAAATTATATTACGAGAAAAATTAGCTATTTTAAAGGTT
ACGAAAGGAAGTAAGGAATA

Downstream 100 bases:

>100_bases
TGGATAATTGTGAAGAAGTATTAAAACTTTTACTAGAAGTTGTTAAAGGTAAAGAATTAAATAATGCAGTTAATAGAAAT
TCATCTGAGATTCCAGTAGG

Product: acetaldehyde dehydrogenase (acetylating)

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MENFDKDLRSIQQARDLARLGKVAADKIADYTEEQIDKILRNMVRVAEENAVCLAQMAVEETGFGKVEDKTFKNHLAATI
LYNSIKDMKTIGVINEDKENKLIELAEPVGLVMGIVPSTNPTSTAIFKAMISIKSRNAIVFAPHPAAAKCTIKAVELMRD
AAIEAGAPENIISAVTIPTIGATNELMKSKEVAIIIATGGPGMVKAAYSSGTPAIGVGAGNSPAYIERTANVEKAVKNII
ASKTFDNGTICASEQSIICEECNHDLVVAEFKKQGGYFMTEEETEKVCKLLFKKGNAMNAKFVGRSPQVIASAAGFTIPA
GTKVLIGRQNGVGEGYPLSFEKLTTVLGFYTVKDWHEACELSIKLLQNGIGHTISIHTEDRDMVMKFARKPASRILVNTG
GTQGGTGASTGLNPSFTLGCGTWGGSSVSENVTPKHLMNIKRVAYGIKDCTNLASNDPTFNCIKTAENCHGVQNQFMNMS
PAQIAAAAEVLNKANNCAENTNTCTECSNENTKNEELLDLVNQIVAAMKGAN

Sequences:

>Translated_532_residues
MENFDKDLRSIQQARDLARLGKVAADKIADYTEEQIDKILRNMVRVAEENAVCLAQMAVEETGFGKVEDKTFKNHLAATI
LYNSIKDMKTIGVINEDKENKLIELAEPVGLVMGIVPSTNPTSTAIFKAMISIKSRNAIVFAPHPAAAKCTIKAVELMRD
AAIEAGAPENIISAVTIPTIGATNELMKSKEVAIIIATGGPGMVKAAYSSGTPAIGVGAGNSPAYIERTANVEKAVKNII
ASKTFDNGTICASEQSIICEECNHDLVVAEFKKQGGYFMTEEETEKVCKLLFKKGNAMNAKFVGRSPQVIASAAGFTIPA
GTKVLIGRQNGVGEGYPLSFEKLTTVLGFYTVKDWHEACELSIKLLQNGIGHTISIHTEDRDMVMKFARKPASRILVNTG
GTQGGTGASTGLNPSFTLGCGTWGGSSVSENVTPKHLMNIKRVAYGIKDCTNLASNDPTFNCIKTAENCHGVQNQFMNMS
PAQIAAAAEVLNKANNCAENTNTCTECSNENTKNEELLDLVNQIVAAMKGAN
>Mature_532_residues
MENFDKDLRSIQQARDLARLGKVAADKIADYTEEQIDKILRNMVRVAEENAVCLAQMAVEETGFGKVEDKTFKNHLAATI
LYNSIKDMKTIGVINEDKENKLIELAEPVGLVMGIVPSTNPTSTAIFKAMISIKSRNAIVFAPHPAAAKCTIKAVELMRD
AAIEAGAPENIISAVTIPTIGATNELMKSKEVAIIIATGGPGMVKAAYSSGTPAIGVGAGNSPAYIERTANVEKAVKNII
ASKTFDNGTICASEQSIICEECNHDLVVAEFKKQGGYFMTEEETEKVCKLLFKKGNAMNAKFVGRSPQVIASAAGFTIPA
GTKVLIGRQNGVGEGYPLSFEKLTTVLGFYTVKDWHEACELSIKLLQNGIGHTISIHTEDRDMVMKFARKPASRILVNTG
GTQGGTGASTGLNPSFTLGCGTWGGSSVSENVTPKHLMNIKRVAYGIKDCTNLASNDPTFNCIKTAENCHGVQNQFMNMS
PAQIAAAAEVLNKANNCAENTNTCTECSNENTKNEELLDLVNQIVAAMKGAN

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787493, Length=431, Percent_Identity=49.1879350348028, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI1788797, Length=368, Percent_Identity=32.0652173913043, Blast_Score=166, Evalue=5e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 56911; Mature: 56911

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENFDKDLRSIQQARDLARLGKVAADKIADYTEEQIDKILRNMVRVAEENAVCLAQMAVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ETGFGKVEDKTFKNHLAATILYNSIKDMKTIGVINEDKENKLIELAEPVGLVMGIVPSTN
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEECCCCCC
PTSTAIFKAMISIKSRNAIVFAPHPAAAKCTIKAVELMRDAAIEAGAPENIISAVTIPTI
CHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHEEECCCC
GATNELMKSKEVAIIIATGGPGMVKAAYSSGTPAIGVGAGNSPAYIERTANVEKAVKNII
CCHHHHHCCCCEEEEEEECCCCEEEEECCCCCCEEEECCCCCCCEEEHHCCHHHHHHHHH
ASKTFDNGTICASEQSIICEECNHDLVVAEFKKQGGYFMTEEETEKVCKLLFKKGNAMNA
HHCCCCCCCEECCCCHHHHHHCCCCEEEEEEHHCCCEEECHHHHHHHHHHHHHCCCCCCE
KFVGRSPQVIASAAGFTIPAGTKVLIGRQNGVGEGYPLSFEKLTTVLGFYTVKDWHEACE
EECCCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LSIKLLQNGIGHTISIHTEDRDMVMKFARKPASRILVNTGGTQGGTGASTGLNPSFTLGC
HHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEC
GTWGGSSVSENVTPKHLMNIKRVAYGIKDCTNLASNDPTFNCIKTAENCHGVQNQFMNMS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
PAQIAAAAEVLNKANNCAENTNTCTECSNENTKNEELLDLVNQIVAAMKGAN
HHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MENFDKDLRSIQQARDLARLGKVAADKIADYTEEQIDKILRNMVRVAEENAVCLAQMAVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ETGFGKVEDKTFKNHLAATILYNSIKDMKTIGVINEDKENKLIELAEPVGLVMGIVPSTN
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEECCCCCC
PTSTAIFKAMISIKSRNAIVFAPHPAAAKCTIKAVELMRDAAIEAGAPENIISAVTIPTI
CHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHEEECCCC
GATNELMKSKEVAIIIATGGPGMVKAAYSSGTPAIGVGAGNSPAYIERTANVEKAVKNII
CCHHHHHCCCCEEEEEEECCCCEEEEECCCCCCEEEECCCCCCCEEEHHCCHHHHHHHHH
ASKTFDNGTICASEQSIICEECNHDLVVAEFKKQGGYFMTEEETEKVCKLLFKKGNAMNA
HHCCCCCCCEECCCCHHHHHHCCCCEEEEEEHHCCCEEECHHHHHHHHHHHHHCCCCCCE
KFVGRSPQVIASAAGFTIPAGTKVLIGRQNGVGEGYPLSFEKLTTVLGFYTVKDWHEACE
EECCCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LSIKLLQNGIGHTISIHTEDRDMVMKFARKPASRILVNTGGTQGGTGASTGLNPSFTLGC
HHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEC
GTWGGSSVSENVTPKHLMNIKRVAYGIKDCTNLASNDPTFNCIKTAENCHGVQNQFMNMS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
PAQIAAAAEVLNKANNCAENTNTCTECSNENTKNEELLDLVNQIVAAMKGAN
HHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]