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The map label for this gene is srlE [H]

Identifier: 153936044

GI number: 153936044

Start: 3529112

End: 3530116

Strand: Reverse

Name: srlE [H]

Synonym: CLC_3360

Alternate gene names: 153936044

Gene position: 3530116-3529112 (Counterclockwise)

Preceding gene: 153936430

Following gene: 153935324

Centisome position: 93.87

GC content: 34.73

Gene sequence:

>1005_bases
ATGGCAAAATATAACGCAATAAAGGTTAGTGCAGGAAATGGCGGTTTTGGAGGCCCCCTTACAATAGTTCCGACAGAGCA
AAGAAATAAAATAGTTAATATAACTGGAGGAGTTATTTCAGACGTAGCAAAAAAATTAGAAGAGCTTACAGGCTGTGAAT
TGGTAGACGGATTTAAAACAAAGGTATCAGATGATGAAATAGCTTGTGTAATAATAGATTGCGGTGGTACATTAAGATGT
GGATTATATCCTAAAAAAGGTATACCAACTATAAACTTAATGCCAGCGGGACCAAGTGGACCATTAGCTGAATATATAAA
AGCAGATATATATGTATCTTCTGTAAAGGTTAAGAATATTGAATTGGCGGATTCTTCTCAAAATAGTGAAAACGTTTATG
AAGAAAATCAACAAAAAGAAGAAGTTAAGAAACCTAAATATGATACAACTAAAAAGATAACCCAACAATCTGATAGAGGT
ATAATGACTAAAATAGGTATGGCTCTTGGTAATTTCGTAGGAATGCTATATCAAGCTGGCCGTGATACTATAGATACTGT
CATAAAAACTATATTACCATTCATGGCATTTGTATGTATGCTAATAGGTATAATATTAAAATCTGGTATAGGAGATGCAT
TTGCATCAGTATTATCTCCATTGGCAGGCAGCTTACCAGGATTAGTATTACTATCAGCTATATGCTCATTCCCATTATTG
TCACCATTTTTAGGACCTGGTGCAGTTATAGCTCAAGTTATAGGTGTTTTAATTGGTGTTGAAATTGGTAAAGGTAATAT
ACCACCACATTTAGCATTACCAGCATTATTTGCAATAAACTCTCAAGCAGCATGTGACTTTATACCAGTAGGTCTTGGAC
TTGCAGAAGCTGAGCCGGAAACAGTAGAAGTTGGAGTACCATCTGTTTTATATTCAAGATTTTTAACAGGCCCAACATCA
GTATTTATTGCATGGCTTGCTAGCTTTGGATTATATGCTAAATAG

Upstream 100 bases:

>100_bases
TTTCCTTAGAGGATGGATAACAGATTTCACAACTTCTTATGTAGAAAAACAACAAAACGTAAAATTATCTACAAAAATTT
CACTTTAGGAGGTGTAATTG

Downstream 100 bases:

>100_bases
ATTTGATTAAAAATAAAATATAAGAATAAAAGGATTGCATTCTTTTATTCTTATATTTAAATAATTTAAATTAATTTTTA
CTTTGCATAATAATAATTTT

Product: PTS system, glucitol/sorbitol-specific, IIBC component

Products: NA

Alternate protein names: EII-Gut; PTS system glucitol/sorbitol-specific EIIB component [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MAKYNAIKVSAGNGGFGGPLTIVPTEQRNKIVNITGGVISDVAKKLEELTGCELVDGFKTKVSDDEIACVIIDCGGTLRC
GLYPKKGIPTINLMPAGPSGPLAEYIKADIYVSSVKVKNIELADSSQNSENVYEENQQKEEVKKPKYDTTKKITQQSDRG
IMTKIGMALGNFVGMLYQAGRDTIDTVIKTILPFMAFVCMLIGIILKSGIGDAFASVLSPLAGSLPGLVLLSAICSFPLL
SPFLGPGAVIAQVIGVLIGVEIGKGNIPPHLALPALFAINSQAACDFIPVGLGLAEAEPETVEVGVPSVLYSRFLTGPTS
VFIAWLASFGLYAK

Sequences:

>Translated_334_residues
MAKYNAIKVSAGNGGFGGPLTIVPTEQRNKIVNITGGVISDVAKKLEELTGCELVDGFKTKVSDDEIACVIIDCGGTLRC
GLYPKKGIPTINLMPAGPSGPLAEYIKADIYVSSVKVKNIELADSSQNSENVYEENQQKEEVKKPKYDTTKKITQQSDRG
IMTKIGMALGNFVGMLYQAGRDTIDTVIKTILPFMAFVCMLIGIILKSGIGDAFASVLSPLAGSLPGLVLLSAICSFPLL
SPFLGPGAVIAQVIGVLIGVEIGKGNIPPHLALPALFAINSQAACDFIPVGLGLAEAEPETVEVGVPSVLYSRFLTGPTS
VFIAWLASFGLYAK
>Mature_333_residues
AKYNAIKVSAGNGGFGGPLTIVPTEQRNKIVNITGGVISDVAKKLEELTGCELVDGFKTKVSDDEIACVIIDCGGTLRCG
LYPKKGIPTINLMPAGPSGPLAEYIKADIYVSSVKVKNIELADSSQNSENVYEENQQKEEVKKPKYDTTKKITQQSDRGI
MTKIGMALGNFVGMLYQAGRDTIDTVIKTILPFMAFVCMLIGIILKSGIGDAFASVLSPLAGSLPGLVLLSAICSFPLLS
PFLGPGAVIAQVIGVLIGVEIGKGNIPPHLALPALFAINSQAACDFIPVGLGLAEAEPETVEVGVPSVLYSRFLTGPTSV
FIAWLASFGLYAK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3732

COG function: function code G; Phosphotransferase system sorbitol-specific component IIBC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI48994905, Length=327, Percent_Identity=60.8562691131498, Blast_Score=371, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011638
- InterPro:   IPR011618
- InterPro:   IPR004702 [H]

Pfam domain/function: PF07663 EIIBC-GUT_C; PF03612 EIIBC-GUT_N [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 35156; Mature: 35024

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS51102 PTS_EIIB_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYNAIKVSAGNGGFGGPLTIVPTEQRNKIVNITGGVISDVAKKLEELTGCELVDGFKT
CCCCCEEEEECCCCCCCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCHHHCCHHC
KVSDDEIACVIIDCGGTLRCGLYPKKGIPTINLMPAGPSGPLAEYIKADIYVSSVKVKNI
CCCCCCEEEEEEECCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHEEEEEEEEEEE
ELADSSQNSENVYEENQQKEEVKKPKYDTTKKITQQSDRGIMTKIGMALGNFVGMLYQAG
EECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
RDTIDTVIKTILPFMAFVCMLIGIILKSGIGDAFASVLSPLAGSLPGLVLLSAICSFPLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SPFLGPGAVIAQVIGVLIGVEIGKGNIPPHLALPALFAINSQAACDFIPVGLGLAEAEPE
HHCCCCHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCC
TVEVGVPSVLYSRFLTGPTSVFIAWLASFGLYAK
EEECCCHHHHHHHHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AKYNAIKVSAGNGGFGGPLTIVPTEQRNKIVNITGGVISDVAKKLEELTGCELVDGFKT
CCCCEEEEECCCCCCCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCHHHCCHHC
KVSDDEIACVIIDCGGTLRCGLYPKKGIPTINLMPAGPSGPLAEYIKADIYVSSVKVKNI
CCCCCCEEEEEEECCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHEEEEEEEEEEE
ELADSSQNSENVYEENQQKEEVKKPKYDTTKKITQQSDRGIMTKIGMALGNFVGMLYQAG
EECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
RDTIDTVIKTILPFMAFVCMLIGIILKSGIGDAFASVLSPLAGSLPGLVLLSAICSFPLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SPFLGPGAVIAQVIGVLIGVEIGKGNIPPHLALPALFAINSQAACDFIPVGLGLAEAEPE
HHCCCCHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCC
TVEVGVPSVLYSRFLTGPTSVFIAWLASFGLYAK
EEECCCHHHHHHHHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9572925 [H]