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The map label for this gene is potB [H]

Identifier: 153935952

GI number: 153935952

Start: 635001

End: 635960

Strand: Direct

Name: potB [H]

Synonym: CLC_0619

Alternate gene names: 153935952

Gene position: 635001-635960 (Clockwise)

Preceding gene: 153936869

Following gene: 153936910

Centisome position: 16.89

GC content: 29.27

Gene sequence:

>960_bases
ATGTGGGGGAAATTTAGCAGTGCTTTTTCAAATATTTTTAAAAAGCAATTGCATAATAAAAATACAGATATTAGCTTAGA
AAGCAAAGAAGGAATAAGAGGTAAACTAAAAAAATCTACCCCTATATTTAAAACAGTGGGGCCCGTTGCCCTATGGCTCA
TTACATTTTTTATTGTTCCTTTACTTCTTGTATTAGTGGTTAGCTTTTTATCAAGAGGTGAATATGGAGATGTAACATTA
CCTTTTACACTAAAAAATTATAAAAGACTCATAGATCCTATGTACGGACAAATTTTAATTAAAACTTTAATGATTTCTAT
TAGTACTACTTTAGGGTGTTTGATTATAGGTTATCCTTTTGCATATTTTATTGGAAGATCAGATAAAAAATATAGAGGAC
TATTATTATTATTAGTTATACTACCTTTTTGGACTAATTCTTTAGTTCGAACTTATGCATGGATAATACTTCTAAAAACA
GAGGGAGTAATTAACACATATCTTATGAGATTTGGATTAATTCACTCCCCAATGCAAATGCTTTATACAGATAGTGCTGT
ATTTATAGGAATGCTTTATATAATGTTGCCTTTTATGGTGCTACCTATTTATACATCTATAGAAAAATTAGATATGAGTT
ATATAGAAGCTGCCAATGATCTTGGAGCATCACCTTACAAGACCTTTAAAGAAATAACATTACCTTTAACTCTACCGGGT
ATTATAGCAGGATGTATGCTAGTATTTATACCAACCTTAGGTTATTTCTTTATATCTGACTTAATGGGTGGAAGTAAAAC
TATGTTAATTTCAAATCTAATTAAAAACCAATTTTTAACCTCTAGAGATTGGCCCTTTGGTTCGGCTATAGCAGTAATAT
TAATTATTTTAATGCTTATAGCTATAAGTTTCTATTTTAAAGGAAATAAACCAAAGAAGGGAATAAAGGAGGTTAACTAA

Upstream 100 bases:

>100_bases
TGGTGTCAGAGCCTATAAGTGATGTATATTCTCTAGACACCCATAATAAAAACTATGTGTTTGCCACATGGAATCCTAAA
CATGCAGTGGTGATGCATTA

Downstream 100 bases:

>100_bases
AGATGAAGAGCAATAAAGGTTTATTAAGTAAAATATATGCAACTTTAGTTTATGTTTTTTTATATTTGCCAATCTTTGTT
TTAGTAGTATTTTCTTTTAA

Product: spermidine/putrescine ABC transporter, permease protein PotB

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MWGKFSSAFSNIFKKQLHNKNTDISLESKEGIRGKLKKSTPIFKTVGPVALWLITFFIVPLLLVLVVSFLSRGEYGDVTL
PFTLKNYKRLIDPMYGQILIKTLMISISTTLGCLIIGYPFAYFIGRSDKKYRGLLLLLVILPFWTNSLVRTYAWIILLKT
EGVINTYLMRFGLIHSPMQMLYTDSAVFIGMLYIMLPFMVLPIYTSIEKLDMSYIEAANDLGASPYKTFKEITLPLTLPG
IIAGCMLVFIPTLGYFFISDLMGGSKTMLISNLIKNQFLTSRDWPFGSAIAVILIILMLIAISFYFKGNKPKKGIKEVN

Sequences:

>Translated_319_residues
MWGKFSSAFSNIFKKQLHNKNTDISLESKEGIRGKLKKSTPIFKTVGPVALWLITFFIVPLLLVLVVSFLSRGEYGDVTL
PFTLKNYKRLIDPMYGQILIKTLMISISTTLGCLIIGYPFAYFIGRSDKKYRGLLLLLVILPFWTNSLVRTYAWIILLKT
EGVINTYLMRFGLIHSPMQMLYTDSAVFIGMLYIMLPFMVLPIYTSIEKLDMSYIEAANDLGASPYKTFKEITLPLTLPG
IIAGCMLVFIPTLGYFFISDLMGGSKTMLISNLIKNQFLTSRDWPFGSAIAVILIILMLIAISFYFKGNKPKKGIKEVN
>Mature_319_residues
MWGKFSSAFSNIFKKQLHNKNTDISLESKEGIRGKLKKSTPIFKTVGPVALWLITFFIVPLLLVLVVSFLSRGEYGDVTL
PFTLKNYKRLIDPMYGQILIKTLMISISTTLGCLIIGYPFAYFIGRSDKKYRGLLLLLVILPFWTNSLVRTYAWIILLKT
EGVINTYLMRFGLIHSPMQMLYTDSAVFIGMLYIMLPFMVLPIYTSIEKLDMSYIEAANDLGASPYKTFKEITLPLTLPG
IIAGCMLVFIPTLGYFFISDLMGGSKTMLISNLIKNQFLTSRDWPFGSAIAVILIILMLIAISFYFKGNKPKKGIKEVN

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=259, Percent_Identity=50.1930501930502, Blast_Score=277, Evalue=7e-76,
Organism=Escherichia coli, GI1787080, Length=244, Percent_Identity=44.2622950819672, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1787713, Length=241, Percent_Identity=32.7800829875519, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1788764, Length=183, Percent_Identity=28.9617486338798, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786980, Length=77, Percent_Identity=42.8571428571429, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36080; Mature: 36080

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]