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The map label for this gene is bioB [H]

Identifier: 153935870

GI number: 153935870

Start: 3698156

End: 3699202

Strand: Direct

Name: bioB [H]

Synonym: CLC_3560

Alternate gene names: 153935870

Gene position: 3698156-3699202 (Clockwise)

Preceding gene: 153934565

Following gene: 153936420

Centisome position: 98.34

GC content: 29.8

Gene sequence:

>1047_bases
ATGAAAAAAATTATTGATGAACTATATGAAAGAAACAATACCTCTAATGATAAACTTTTATATTTACTTAACAATATTGA
TAAAGAAAATAAAAAATATCTAATATATAAAGCTCATGAAACTAGTTTAAAGCACTATAATAGCAAAGTTTATTTAAGGG
GATTAATAGAATTTACAAACTATTGTAAAAATGCTTGTCTATATTGTGGTATATCTTGTAATAATAACAATGCTGAAAGA
TATAGACTATCTGAAGAAGAAATACTTAACTGTTGCAAGAAAGGCTACTCTTTAGGCTATAGAACTTTTGTACTCCAAGG
TGGAGAAGACCCTTACTATACAGATAATAAAATTATTGACATTATAAAATCTATAAAAAAATTATTTCCAAACTGTGCTG
TAACATTATCTATTGGCGAAAAAAGCTACGAAACATATAAAAAATTTTATGACGCAGGGGCAGATAGATATTTACTAAGG
CATGAAACTGCTTCTAAAAATCTATACGAAAAACTTCATCCTGATATGAGTTTTGAAAATAGACACTCATGCTTAAGAAA
CTTAAAAAAAATAGGCTATCAAGTTGGTGCTGGTTTCATGATAGGACTACCTAGTCAAACAAATGAAGACTATGTAAATG
ACTTGATATTTTTAAAAGAATTAAAACCTCATATGGTTGGTATAGGTCCTTTTATTCCTCATAAAGACACTGTACTTAAA
AATCAATCTGCTGGTACATTAGAAGATACTACCACATTATTAGCTCTTATAAGGTTGCTTTTACCTGAGGTTTTATTACC
CGCAACTACAGCTCTAGGAACAATAAATCCTATGGGACGGGAAGCAGGTTTAAAAGCTGGAGCTAATGTGGTTATGCCAA
ATCTTTCTCCCACTAACGTTAGAGATAAATATATGCTATATGATAATAAAATTTGTACTGGTGATGAAGCTGCAGAGTGC
AGGAAATGCATAGAAAATAGAATTAATAATGCTGGTTTTTCTCTAGACTTAACTAGAGGAGATAATATTTTATGGAGGCG
AATATAA

Upstream 100 bases:

>100_bases
TAACTGGAACTCTAGATGAAATTAATAGCCTTACAGGTAAATTAGGAAATCTAGAAAATGTATATGTAAAGACATCTGTA
TCAAAGAAAGAAATATAATT

Downstream 100 bases:

>100_bases
ATGTTTATAAATCATGATTATATAGAAAATTTATTAAAAGAGGCTGAAAATGCTACAAATGAAGATATAGAAAAGGTTTT
AGATAAAGCCTGTAGTAATG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKKIIDELYERNNTSNDKLLYLLNNIDKENKKYLIYKAHETSLKHYNSKVYLRGLIEFTNYCKNACLYCGISCNNNNAER
YRLSEEEILNCCKKGYSLGYRTFVLQGGEDPYYTDNKIIDIIKSIKKLFPNCAVTLSIGEKSYETYKKFYDAGADRYLLR
HETASKNLYEKLHPDMSFENRHSCLRNLKKIGYQVGAGFMIGLPSQTNEDYVNDLIFLKELKPHMVGIGPFIPHKDTVLK
NQSAGTLEDTTTLLALIRLLLPEVLLPATTALGTINPMGREAGLKAGANVVMPNLSPTNVRDKYMLYDNKICTGDEAAEC
RKCIENRINNAGFSLDLTRGDNILWRRI

Sequences:

>Translated_348_residues
MKKIIDELYERNNTSNDKLLYLLNNIDKENKKYLIYKAHETSLKHYNSKVYLRGLIEFTNYCKNACLYCGISCNNNNAER
YRLSEEEILNCCKKGYSLGYRTFVLQGGEDPYYTDNKIIDIIKSIKKLFPNCAVTLSIGEKSYETYKKFYDAGADRYLLR
HETASKNLYEKLHPDMSFENRHSCLRNLKKIGYQVGAGFMIGLPSQTNEDYVNDLIFLKELKPHMVGIGPFIPHKDTVLK
NQSAGTLEDTTTLLALIRLLLPEVLLPATTALGTINPMGREAGLKAGANVVMPNLSPTNVRDKYMLYDNKICTGDEAAEC
RKCIENRINNAGFSLDLTRGDNILWRRI
>Mature_348_residues
MKKIIDELYERNNTSNDKLLYLLNNIDKENKKYLIYKAHETSLKHYNSKVYLRGLIEFTNYCKNACLYCGISCNNNNAER
YRLSEEEILNCCKKGYSLGYRTFVLQGGEDPYYTDNKIIDIIKSIKKLFPNCAVTLSIGEKSYETYKKFYDAGADRYLLR
HETASKNLYEKLHPDMSFENRHSCLRNLKKIGYQVGAGFMIGLPSQTNEDYVNDLIFLKELKPHMVGIGPFIPHKDTVLK
NQSAGTLEDTTTLLALIRLLLPEVLLPATTALGTINPMGREAGLKAGANVVMPNLSPTNVRDKYMLYDNKICTGDEAAEC
RKCIENRINNAGFSLDLTRGDNILWRRI

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 39752; Mature: 39752

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIIDELYERNNTSNDKLLYLLNNIDKENKKYLIYKAHETSLKHYNSKVYLRGLIEFTN
CHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEECCHHHHHHCCCHHHHHHHHHHHH
YCKNACLYCGISCNNNNAERYRLSEEEILNCCKKGYSLGYRTFVLQGGEDPYYTDNKIID
HHCCCCEEEEEEECCCCHHHEECCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHH
IIKSIKKLFPNCAVTLSIGEKSYETYKKFYDAGADRYLLRHETASKNLYEKLHPDMSFEN
HHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCCH
RHSCLRNLKKIGYQVGAGFMIGLPSQTNEDYVNDLIFLKELKPHMVGIGPFIPHKDTVLK
HHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCHHEECCCCCCCCCHHCC
NQSAGTLEDTTTLLALIRLLLPEVLLPATTALGTINPMGREAGLKAGANVVMPNLSPTNV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEECCCCCCCC
RDKYMLYDNKICTGDEAAECRKCIENRINNAGFSLDLTRGDNILWRRI
CCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEECC
>Mature Secondary Structure
MKKIIDELYERNNTSNDKLLYLLNNIDKENKKYLIYKAHETSLKHYNSKVYLRGLIEFTN
CHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEECCHHHHHHCCCHHHHHHHHHHHH
YCKNACLYCGISCNNNNAERYRLSEEEILNCCKKGYSLGYRTFVLQGGEDPYYTDNKIID
HHCCCCEEEEEEECCCCHHHEECCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHH
IIKSIKKLFPNCAVTLSIGEKSYETYKKFYDAGADRYLLRHETASKNLYEKLHPDMSFEN
HHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCCH
RHSCLRNLKKIGYQVGAGFMIGLPSQTNEDYVNDLIFLKELKPHMVGIGPFIPHKDTVLK
HHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCHHEECCCCCCCCCHHCC
NQSAGTLEDTTTLLALIRLLLPEVLLPATTALGTINPMGREAGLKAGANVVMPNLSPTNV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEECCCCCCCC
RDKYMLYDNKICTGDEAAECRKCIENRINNAGFSLDLTRGDNILWRRI
CCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA