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The map label for this gene is cobS

Identifier: 153935741

GI number: 153935741

Start: 903286

End: 904032

Strand: Direct

Name: cobS

Synonym: CLC_0876

Alternate gene names: 153935741

Gene position: 903286-904032 (Clockwise)

Preceding gene: 153934760

Following gene: 153935846

Centisome position: 24.02

GC content: 25.03

Gene sequence:

>747_bases
ATGAAGAGTATATTAAATGATTTTTTATTAATGATTCAATTTTTTACACGTATACCTGTAAATAAAAACTTACAATGTGA
AAAAGTAAATTTTAGAAGGGGAGCTTTTTTTCTTCCTGTAGTAGCTTCTATTATAGGTGGAATAGAGTTTTTAGTATATT
TAGGCCTTAAAAATTTTTTACCACCTAATGTAATAATAGTGTTATTGCTTTTATTTACAGCTATGATTACTGGGGGCCTT
CATATGGATGGATTAGCAGATACCTGTGATGGATTTTTTTCTTTAAGAGATAAGGAAAGAATAATAGAAATTATGAAAGA
TAGCAGAATGGGGGCTTTTGGAACTATAGCAATGATAATTAATTTATTACTTAAATACCAGCTGCTTTATTCCTTAGTTT
TAAAAGACTGTAGTATAGCTATAATTTTAGCACCTGTTATAGGAAGAATTAGTATTTTATTTTTATGCTTATCTAAAAGA
ACAGCAAAAAAGAATGGTTCAGGAAATATATTTATAGGAAATATGAGTAAACCTATAATATTTCTTATTACTATAATAGC
ATTAGCAATGAATACATATTTTTTAGGATTAAAAATTACAATAATTTCATTTATAGCAGTGTTAATAATAACATATTTAT
TTTATTTATTATGTTTAAATAAAATAAATGGACTTACAGGTGATACCTTGGGAGCTTGTAATGAACTAGGTGAAATAACA
TTTTTATTAATTTTATTAATGATGTAA

Upstream 100 bases:

>100_bases
ATCCATTATTTATTTTTGGAAAACTATAATATGGAATTTAATAAATTTTTGTTTATACGAAATTTAGGTAATTAATAATA
AATATAAAAGAGGGGGAAAC

Downstream 100 bases:

>100_bases
AAACTTTAATAGATGGTGTTTCAAAATAAAATAGATTTAATTTTAAAGGCGCAAAGATAAAAAATACACTCATAAATCAC
TTCATTTCGCTAAGAAGTTC

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKSILNDFLLMIQFFTRIPVNKNLQCEKVNFRRGAFFLPVVASIIGGIEFLVYLGLKNFLPPNVIIVLLLLFTAMITGGL
HMDGLADTCDGFFSLRDKERIIEIMKDSRMGAFGTIAMIINLLLKYQLLYSLVLKDCSIAIILAPVIGRISILFLCLSKR
TAKKNGSGNIFIGNMSKPIIFLITIIALAMNTYFLGLKITIISFIAVLIITYLFYLLCLNKINGLTGDTLGACNELGEIT
FLLILLMM

Sequences:

>Translated_248_residues
MKSILNDFLLMIQFFTRIPVNKNLQCEKVNFRRGAFFLPVVASIIGGIEFLVYLGLKNFLPPNVIIVLLLLFTAMITGGL
HMDGLADTCDGFFSLRDKERIIEIMKDSRMGAFGTIAMIINLLLKYQLLYSLVLKDCSIAIILAPVIGRISILFLCLSKR
TAKKNGSGNIFIGNMSKPIIFLITIIALAMNTYFLGLKITIISFIAVLIITYLFYLLCLNKINGLTGDTLGACNELGEIT
FLLILLMM
>Mature_248_residues
MKSILNDFLLMIQFFTRIPVNKNLQCEKVNFRRGAFFLPVVASIIGGIEFLVYLGLKNFLPPNVIIVLLLLFTAMITGGL
HMDGLADTCDGFFSLRDKERIIEIMKDSRMGAFGTIAMIINLLLKYQLLYSLVLKDCSIAIILAPVIGRISILFLCLSKR
TAKKNGSGNIFIGNMSKPIIFLITIIALAMNTYFLGLKITIISFIAVLIITYLFYLLCLNKINGLTGDTLGACNELGEIT
FLLILLMM

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=247, Percent_Identity=34.8178137651822, Blast_Score=138, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_CLOB1 (A7FS72)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001383199.1
- STRING:   A7FS72
- GeneID:   5397582
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0862
- eggNOG:   COG0368
- HOGENOM:   HBG729392
- OMA:   IMKDPTS
- ProtClustDB:   PRK00235
- BioCyc:   CBOT441770:CLB_0862-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 27621; Mature: 27621

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1a9c5bb4)-; HASH(0x1ac7bc94)-; HASH(0x1a8ed5fc)-; HASH(0x1a92f750)-; HASH(0x1a4f3bc0)-; HASH(0x18a27b50)-;

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSILNDFLLMIQFFTRIPVNKNLQCEKVNFRRGAFFLPVVASIIGGIEFLVYLGLKNFL
CHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PPNVIIVLLLLFTAMITGGLHMDGLADTCDGFFSLRDKERIIEIMKDSRMGAFGTIAMII
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NLLLKYQLLYSLVLKDCSIAIILAPVIGRISILFLCLSKRTAKKNGSGNIFIGNMSKPII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHH
FLITIIALAMNTYFLGLKITIISFIAVLIITYLFYLLCLNKINGLTGDTLGACNELGEIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FLLILLMM
HHHHHHHH
>Mature Secondary Structure
MKSILNDFLLMIQFFTRIPVNKNLQCEKVNFRRGAFFLPVVASIIGGIEFLVYLGLKNFL
CHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PPNVIIVLLLLFTAMITGGLHMDGLADTCDGFFSLRDKERIIEIMKDSRMGAFGTIAMII
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NLLLKYQLLYSLVLKDCSIAIILAPVIGRISILFLCLSKRTAKKNGSGNIFIGNMSKPII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHH
FLITIIALAMNTYFLGLKITIISFIAVLIITYLFYLLCLNKINGLTGDTLGACNELGEIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FLLILLMM
HHHHHHHH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA