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The map label for this gene is cobB [H]

Identifier: 153935622

GI number: 153935622

Start: 74540

End: 75283

Strand: Direct

Name: cobB [H]

Synonym: CLC_0094

Alternate gene names: 153935622

Gene position: 74540-75283 (Clockwise)

Preceding gene: 153936847

Following gene: 153936188

Centisome position: 1.98

GC content: 26.88

Gene sequence:

>744_bases
ATGAGGCTAGAAAAACTAAAACATATTATAGATAATAGTGAAAATATAGTGTTTTTAGGTGGAGCAGGAGTGTCTACAGA
AAGTAATATTCCGGATTTCAGATCAAATAATGGATTATACAAATCAAAAAATAATTTGAATTATTCACCAGAAACAATAT
TAAGTCATAGTTTTTTTAAGAATAATGCTAAAGAATTTTTTCAATTTTATAAAGATAAGATGATATTTAAAGATGCAAAG
CCTAATTTAGCCCATTATGCTTTAACAGAGTTAGAACAGATAGGAAAACTAAAGACTATAATAACGCAAAATATTGATGG
ATTACATCAGTTATCAGGAGCTAAGAATGTATTAGAGTTACATGGAAGTGTGCATAGAAATTATTGTGTTAATTGTGGAG
AAAAATATAATTTAGATTATATATTAAATACTGAAAATAGTAGTGAAGATATACCGTATTGTAAAAAATGTGGAAGTATA
GTTAGACCAGATGTAGTGCTTTATGAAGAAGGATTAGACATGGATACTATAAGCAAAGCCATATATTATATACAAAATGC
AGATGTGCTTATAGTAGGTGGTACATCACTTGTAGTATATCCTGCTGCTGGATTAGTTAATTATTATAAAGGTAAAAAAT
TAGTTTTAATAAATAAAGCAGAAACACCTTATGACAAAAAAGCTGACTTAGTTATTCATGATTCTATAGGCAGTGTTTTA
GAAAAAGTAATTAAAAAAAACTAA

Upstream 100 bases:

>100_bases
TTTATAGTACTTATTTCTTCTTTAGGAGCAATTAAGTTTTAATAATTGTATTATTAGTATATAATAATTATGATTAATAA
CCTATTTAGGAGATGATAAT

Downstream 100 bases:

>100_bases
TCAAATTTTTGGTTAGTTTTTTAATTTTAATATTGACAAGTTTTAAATTTCTGATATAATAAATTATGTTGAATTGAATA
TGGCGCTATAGCCAAGTGGT

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MRLEKLKHIIDNSENIVFLGGAGVSTESNIPDFRSNNGLYKSKNNLNYSPETILSHSFFKNNAKEFFQFYKDKMIFKDAK
PNLAHYALTELEQIGKLKTIITQNIDGLHQLSGAKNVLELHGSVHRNYCVNCGEKYNLDYILNTENSSEDIPYCKKCGSI
VRPDVVLYEEGLDMDTISKAIYYIQNADVLIVGGTSLVVYPAAGLVNYYKGKKLVLINKAETPYDKKADLVIHDSIGSVL
EKVIKKN

Sequences:

>Translated_247_residues
MRLEKLKHIIDNSENIVFLGGAGVSTESNIPDFRSNNGLYKSKNNLNYSPETILSHSFFKNNAKEFFQFYKDKMIFKDAK
PNLAHYALTELEQIGKLKTIITQNIDGLHQLSGAKNVLELHGSVHRNYCVNCGEKYNLDYILNTENSSEDIPYCKKCGSI
VRPDVVLYEEGLDMDTISKAIYYIQNADVLIVGGTSLVVYPAAGLVNYYKGKKLVLINKAETPYDKKADLVIHDSIGSVL
EKVIKKN
>Mature_247_residues
MRLEKLKHIIDNSENIVFLGGAGVSTESNIPDFRSNNGLYKSKNNLNYSPETILSHSFFKNNAKEFFQFYKDKMIFKDAK
PNLAHYALTELEQIGKLKTIITQNIDGLHQLSGAKNVLELHGSVHRNYCVNCGEKYNLDYILNTENSSEDIPYCKKCGSI
VRPDVVLYEEGLDMDTISKAIYYIQNADVLIVGGTSLVVYPAAGLVNYYKGKKLVLINKAETPYDKKADLVIHDSIGSVL
EKVIKKN

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI6912662, Length=272, Percent_Identity=29.0441176470588, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI6912664, Length=267, Percent_Identity=28.4644194756554, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI13787215, Length=246, Percent_Identity=30.0813008130081, Blast_Score=120, Evalue=9e-28,
Organism=Homo sapiens, GI300795542, Length=254, Percent_Identity=28.740157480315, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI300797577, Length=253, Percent_Identity=29.6442687747036, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI6912660, Length=212, Percent_Identity=35.8490566037736, Blast_Score=117, Evalue=7e-27,
Organism=Homo sapiens, GI13775602, Length=217, Percent_Identity=35.4838709677419, Blast_Score=117, Evalue=9e-27,
Organism=Homo sapiens, GI13775600, Length=217, Percent_Identity=35.4838709677419, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI63054862, Length=205, Percent_Identity=36.5853658536585, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI300797597, Length=250, Percent_Identity=29.6, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI300797705, Length=194, Percent_Identity=35.5670103092784, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI7657575, Length=234, Percent_Identity=29.0598290598291, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI7706712, Length=259, Percent_Identity=26.6409266409266, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI215982798, Length=175, Percent_Identity=29.1428571428571, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI308199517, Length=201, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17541892, Length=216, Percent_Identity=32.8703703703704, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71990482, Length=281, Percent_Identity=28.8256227758007, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17567771, Length=276, Percent_Identity=30.0724637681159, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71990487, Length=283, Percent_Identity=28.6219081272085, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17505510, Length=241, Percent_Identity=28.6307053941909, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325242, Length=257, Percent_Identity=31.5175097276265, Blast_Score=105, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6324599, Length=295, Percent_Identity=27.7966101694915, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320163, Length=250, Percent_Identity=28.4, Blast_Score=72, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324504, Length=252, Percent_Identity=28.1746031746032, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648389, Length=210, Percent_Identity=36.6666666666667, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI28571445, Length=273, Percent_Identity=28.5714285714286, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17137536, Length=223, Percent_Identity=30.4932735426009, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24645650, Length=257, Percent_Identity=29.5719844357977, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24650933, Length=266, Percent_Identity=25.9398496240602, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27792; Mature: 27792

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLEKLKHIIDNSENIVFLGGAGVSTESNIPDFRSNNGLYKSKNNLNYSPETILSHSFFK
CCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHH
NNAKEFFQFYKDKMIFKDAKPNLAHYALTELEQIGKLKTIITQNIDGLHQLSGAKNVLEL
CCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHH
HGSVHRNYCVNCGEKYNLDYILNTENSSEDIPYCKKCGSIVRPDVVLYEEGLDMDTISKA
HHHHHCCHHCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCEEEEECCCCHHHHHHH
IYYIQNADVLIVGGTSLVVYPAAGLVNYYKGKKLVLINKAETPYDKKADLVIHDSIGSVL
HHEEECCCEEEECCCEEEEECCHHHHHHHCCCEEEEEECCCCCCCCCCCEEEECCHHHHH
EKVIKKN
HHHHHCC
>Mature Secondary Structure
MRLEKLKHIIDNSENIVFLGGAGVSTESNIPDFRSNNGLYKSKNNLNYSPETILSHSFFK
CCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHH
NNAKEFFQFYKDKMIFKDAKPNLAHYALTELEQIGKLKTIITQNIDGLHQLSGAKNVLEL
CCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHH
HGSVHRNYCVNCGEKYNLDYILNTENSSEDIPYCKKCGSIVRPDVVLYEEGLDMDTISKA
HHHHHCCHHCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCEEEEECCCCHHHHHHH
IYYIQNADVLIVGGTSLVVYPAAGLVNYYKGKKLVLINKAETPYDKKADLVIHDSIGSVL
HHEEECCCEEEECCCEEEEECCHHHHHHHCCCEEEEEECCCCCCCCCCCEEEECCHHHHH
EKVIKKN
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]