| Definition | Clostridium botulinum A str. Hall, complete genome. |
|---|---|
| Accession | NC_009698 |
| Length | 3,760,560 |
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The map label for this gene is arsA [H]
Identifier: 153935358
GI number: 153935358
Start: 830089
End: 831834
Strand: Direct
Name: arsA [H]
Synonym: CLC_0812
Alternate gene names: 153935358
Gene position: 830089-831834 (Clockwise)
Preceding gene: 153937017
Following gene: 153934633
Centisome position: 22.07
GC content: 31.39
Gene sequence:
>1746_bases ATGTTTAAAAAATTTAATGTGGAAGATATAAACTTAACAAAATATTTGTTCTTTACTGGCAAAGGTGGAGTTGGTAAGAC TTCAACAGCTTGTGCTGTAGCAGTAACTTTAGCTGATAAAGGTAAAAAAATTATGCTTGTTAGTACAGATCCAGCTTCAA ATCTTCAAGATGTTTTTAATACAAAATTAAATAATAAGGGAGTTACTATAAAAGAAGTTCCTAATTTGGTAGTTGCAAAC TTTGAACCAGAAGAAGCAGCTGCTGAATATAGAGAAAGTGTAATAGCACCTTATCGTGGAAAATTACCAGAAGCAGTTTT GAAAAATATGGAGGAACAGCTTTCAGGTTCATGTACTGTTGAGATTGCTGCATTTAATGAATTCTCAACCTTTATAACTG ATGAAAAAGTAGAAAAAGAGTATGACCATATTATATTTGATACAGCACCAACAGGTCATACACTAAGGATGTTGCAGCTT CCGTCAGCATGGAGCAATTTTATTAATGAAAGTACACATGGTGCTTCATGTTTAGGACAGCTTTCAGGACTTGAAAGTAA AAAAGAAGTTTATAAAAATGCAGTAAATACTCTAGCGGATAAGGATAAAACTACACTTATACTTGTATCACGTCCAGAAG TATCACCTTTAAAGGAAGCTGAAAGAGCTTCTAAAGAACTTCAAGATATAGGGGTAAACAATCAAGTTTTAGTTATTAAT GGAGTACTTGAAGAGCATGAAGATTATCTTTCTAATGCTATTTATACTAAACAACAAAAAGCATTAGAAGACATTCCAGA ATCACTAAAGATCGTTGAAACTTTTCAAATTCCATTAAGACCTTATAATGTAACAGGTTTAGAAAATCTAAGAGCTTTTT TAAAGAATAATAATATTAAATATAATGATGAAAAATTAAATACTACTAATATACCTAAATTAAATAAGGTAATAGAAGAT TTATATAATACTGATAAAAAAGTAATATTTACAATGGGTAAAGGTGGTGTAGGTAAAACTACAATAGCTGCTGCAATTGC TGTAGGATTAGCTAAAAAAGGAAAGAAAGTACATTTAACAACTACTGATCCAGCAGATCATTTAAAATTTGTGTTAGATG AGGATTATGGAATAACTCTAAGCCATATAGACGAAAAAAAGGAACTAGAAAAATACAAAGAAGAGGTATTAATTAAAGCA CGAAAAACCATGAGTGAAGATGATATCGCTTATGTTGAAGAAGATTTAAGATCACCTTGTACTCAAGAAATTGCAGTATT TAGAGCATTTGCAGAAATAGTTGAAAGGTCAGAAAATGAGGTTGTAGTTATTGATACAGCACCAACAGGACACACTTTAT TACTTTTAGACTGTACTCAAAGTTATAATAAAGAAATTCAACGTTCACAAGGAGATATTCCAAAATCAGTTAAGAAGCTT TTACCTAAGCTTAGAAATGAGGAGCATACAGAAGTAATTATCGTAACATTAGCTGAAACAACTCCAGTTTATGAGGCTAT GAGACTTCAGGAAGATTTGAATAGAGCTGGCATTCACAGTAAATGGTGGGTTATCAATTCAAGTTTTTATGTAGCTGATA CAACAAATTCTATCTTAAAGGTAAAAGCCAATAATGAGATACAGTGGATTAATAAAGTGAATGAAATATCTAAAGGCAAT TTTGCAGTTATAGAGTGGATGCCTGAAGAGGTTAAGGGGGAGATGCTTAACCGCTTAATAAAATAA
Upstream 100 bases:
>100_bases GTTAGATATACCAGAGAATTCTTTAAAAATTACTATAAAAAAACCATCTAAAGGTTGTAATTGTAAAGGTGGATGTTGCT AAAAAGAAAAGAGGGATATA
Downstream 100 bases:
>100_bases GCGTAATTCTACTATAAAGTGACAGTACAAATTTTGTAGTGGAATTTTTAGAATATTATATAGGGCATGATTAGAATATT AATTTAGGGAGGGATAATAT
Product: arsenical pump-driving ATPase ArsA
Products: NA
Alternate protein names: Arsenical resistance ATPase; Arsenite-translocating ATPase; Arsenite-transporting ATPase [H]
Number of amino acids: Translated: 581; Mature: 581
Protein sequence:
>581_residues MFKKFNVEDINLTKYLFFTGKGGVGKTSTACAVAVTLADKGKKIMLVSTDPASNLQDVFNTKLNNKGVTIKEVPNLVVAN FEPEEAAAEYRESVIAPYRGKLPEAVLKNMEEQLSGSCTVEIAAFNEFSTFITDEKVEKEYDHIIFDTAPTGHTLRMLQL PSAWSNFINESTHGASCLGQLSGLESKKEVYKNAVNTLADKDKTTLILVSRPEVSPLKEAERASKELQDIGVNNQVLVIN GVLEEHEDYLSNAIYTKQQKALEDIPESLKIVETFQIPLRPYNVTGLENLRAFLKNNNIKYNDEKLNTTNIPKLNKVIED LYNTDKKVIFTMGKGGVGKTTIAAAIAVGLAKKGKKVHLTTTDPADHLKFVLDEDYGITLSHIDEKKELEKYKEEVLIKA RKTMSEDDIAYVEEDLRSPCTQEIAVFRAFAEIVERSENEVVVIDTAPTGHTLLLLDCTQSYNKEIQRSQGDIPKSVKKL LPKLRNEEHTEVIIVTLAETTPVYEAMRLQEDLNRAGIHSKWWVINSSFYVADTTNSILKVKANNEIQWINKVNEISKGN FAVIEWMPEEVKGEMLNRLIK
Sequences:
>Translated_581_residues MFKKFNVEDINLTKYLFFTGKGGVGKTSTACAVAVTLADKGKKIMLVSTDPASNLQDVFNTKLNNKGVTIKEVPNLVVAN FEPEEAAAEYRESVIAPYRGKLPEAVLKNMEEQLSGSCTVEIAAFNEFSTFITDEKVEKEYDHIIFDTAPTGHTLRMLQL PSAWSNFINESTHGASCLGQLSGLESKKEVYKNAVNTLADKDKTTLILVSRPEVSPLKEAERASKELQDIGVNNQVLVIN GVLEEHEDYLSNAIYTKQQKALEDIPESLKIVETFQIPLRPYNVTGLENLRAFLKNNNIKYNDEKLNTTNIPKLNKVIED LYNTDKKVIFTMGKGGVGKTTIAAAIAVGLAKKGKKVHLTTTDPADHLKFVLDEDYGITLSHIDEKKELEKYKEEVLIKA RKTMSEDDIAYVEEDLRSPCTQEIAVFRAFAEIVERSENEVVVIDTAPTGHTLLLLDCTQSYNKEIQRSQGDIPKSVKKL LPKLRNEEHTEVIIVTLAETTPVYEAMRLQEDLNRAGIHSKWWVINSSFYVADTTNSILKVKANNEIQWINKVNEISKGN FAVIEWMPEEVKGEMLNRLIK >Mature_581_residues MFKKFNVEDINLTKYLFFTGKGGVGKTSTACAVAVTLADKGKKIMLVSTDPASNLQDVFNTKLNNKGVTIKEVPNLVVAN FEPEEAAAEYRESVIAPYRGKLPEAVLKNMEEQLSGSCTVEIAAFNEFSTFITDEKVEKEYDHIIFDTAPTGHTLRMLQL PSAWSNFINESTHGASCLGQLSGLESKKEVYKNAVNTLADKDKTTLILVSRPEVSPLKEAERASKELQDIGVNNQVLVIN GVLEEHEDYLSNAIYTKQQKALEDIPESLKIVETFQIPLRPYNVTGLENLRAFLKNNNIKYNDEKLNTTNIPKLNKVIED LYNTDKKVIFTMGKGGVGKTTIAAAIAVGLAKKGKKVHLTTTDPADHLKFVLDEDYGITLSHIDEKKELEKYKEEVLIKA RKTMSEDDIAYVEEDLRSPCTQEIAVFRAFAEIVERSENEVVVIDTAPTGHTLLLLDCTQSYNKEIQRSQGDIPKSVKKL LPKLRNEEHTEVIIVTLAETTPVYEAMRLQEDLNRAGIHSKWWVINSSFYVADTTNSILKVKANNEIQWINKVNEISKGN FAVIEWMPEEVKGEMLNRLIK
Specific function: Anion-transporting ATPase. Catalyzes the extrusion of the oxyanions arsenite, antimonite and arsenate. Maintenance of a low intracellular concentration of oxyanion produces resistance to the toxic agents [H]
COG id: COG0003
COG function: function code P; Oxyanion-translocating ATPase
Gene ontology:
Cell location: Inner Membrane-Associated [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the arsA ATPase family [H]
Homologues:
Organism=Homo sapiens, GI50428938, Length=307, Percent_Identity=28.0130293159609, Blast_Score=88, Evalue=2e-17, Organism=Caenorhabditis elegans, GI17557003, Length=323, Percent_Identity=25.6965944272446, Blast_Score=71, Evalue=1e-12, Organism=Saccharomyces cerevisiae, GI6320103, Length=324, Percent_Identity=29.320987654321, Blast_Score=103, Evalue=1e-22, Organism=Drosophila melanogaster, GI24586297, Length=317, Percent_Identity=25.2365930599369, Blast_Score=79, Evalue=6e-15,
Paralogues:
None
Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR016300 - InterPro: IPR003593 - InterPro: IPR003348 [H]
Pfam domain/function: NA
EC number: =3.6.3.16 [H]
Molecular weight: Translated: 65193; Mature: 65193
Theoretical pI: Translated: 5.39; Mature: 5.39
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 1.5 %Met (Translated Protein) 2.4 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 2.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MFKKFNVEDINLTKYLFFTGKGGVGKTSTACAVAVTLADKGKKIMLVSTDPASNLQDVFN CCCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH TKLNNKGVTIKEVPNLVVANFEPEEAAAEYRESVIAPYRGKLPEAVLKNMEEQLSGSCTV CCCCCCCEEHHHCCCEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEE EIAAFNEFSTFITDEKVEKEYDHIIFDTAPTGHTLRMLQLPSAWSNFINESTHGASCLGQ EEEEECCHHHHHCHHHHHHHHCEEEEECCCCCCEEEEEECCHHHHHHHCCCCCHHHHHHH LSGLESKKEVYKNAVNTLADKDKTTLILVSRPEVSPLKEAERASKELQDIGVNNQVLVIN HHCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEE GVLEEHEDYLSNAIYTKQQKALEDIPESLKIVETFQIPLRPYNVTGLENLRAFLKNNNIK CHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHEEECCCCCCCCCCHHHHHHHHHCCCCE YNDEKLNTTNIPKLNKVIEDLYNTDKKVIFTMGKGGVGKTTIAAAIAVGLAKKGKKVHLT ECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEE TTDPADHLKFVLDEDYGITLSHIDEKKELEKYKEEVLIKARKTMSEDDIAYVEEDLRSPC ECCCHHHHEEEECCCCCEEHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCH TQEIAVFRAFAEIVERSENEVVVIDTAPTGHTLLLLDCTQSYNKEIQRSQGDIPKSVKKL HHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHHH LPKLRNEEHTEVIIVTLAETTPVYEAMRLQEDLNRAGIHSKWWVINSSFYVADTTNSILK HHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECEEEEEECCCCEEE VKANNEIQWINKVNEISKGNFAVIEWMPEEVKGEMLNRLIK EECCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCC >Mature Secondary Structure MFKKFNVEDINLTKYLFFTGKGGVGKTSTACAVAVTLADKGKKIMLVSTDPASNLQDVFN CCCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH TKLNNKGVTIKEVPNLVVANFEPEEAAAEYRESVIAPYRGKLPEAVLKNMEEQLSGSCTV CCCCCCCEEHHHCCCEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEE EIAAFNEFSTFITDEKVEKEYDHIIFDTAPTGHTLRMLQLPSAWSNFINESTHGASCLGQ EEEEECCHHHHHCHHHHHHHHCEEEEECCCCCCEEEEEECCHHHHHHHCCCCCHHHHHHH LSGLESKKEVYKNAVNTLADKDKTTLILVSRPEVSPLKEAERASKELQDIGVNNQVLVIN HHCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEE GVLEEHEDYLSNAIYTKQQKALEDIPESLKIVETFQIPLRPYNVTGLENLRAFLKNNNIK CHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHEEECCCCCCCCCCHHHHHHHHHCCCCE YNDEKLNTTNIPKLNKVIEDLYNTDKKVIFTMGKGGVGKTTIAAAIAVGLAKKGKKVHLT ECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEE TTDPADHLKFVLDEDYGITLSHIDEKKELEKYKEEVLIKARKTMSEDDIAYVEEDLRSPC ECCCHHHHEEEECCCCCEEHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCH TQEIAVFRAFAEIVERSENEVVVIDTAPTGHTLLLLDCTQSYNKEIQRSQGDIPKSVKKL HHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHHH LPKLRNEEHTEVIIVTLAETTPVYEAMRLQEDLNRAGIHSKWWVINSSFYVADTTNSILK HHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECEEEEEECCCCEEE VKANNEIQWINKVNEISKGNFAVIEWMPEEVKGEMLNRLIK EECCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 3021763; 1704144 [H]