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The map label for this gene is tpiA

Identifier: 153935201

GI number: 153935201

Start: 262741

End: 263487

Strand: Direct

Name: tpiA

Synonym: CLC_0284

Alternate gene names: 153935201

Gene position: 262741-263487 (Clockwise)

Preceding gene: 153937674

Following gene: 153936169

Centisome position: 6.99

GC content: 32.8

Gene sequence:

>747_bases
ATGAGAACAGCTATAATAGCAGGAAACTGGAAGATGAACAAAACTGTAAAAGAAGCAGTAGAATTGGTTAAAGAATTAAA
ACCACTTGTTAAAGATGCAAAATGTGATGTAGTTGTATGTCCTACTTATGTATGCCTTCCAGCGGTATTAGAAGAAGTTA
AAGGAAGCAATATAAAAGTAGGTGCTCAAAATATGCACTTTGAAGAAAGTGGAGCTTACACAGGTGAAATAGCACCTAAA
ATGTTAGAAGAACTAGGAGTTCATTATGTAATTATAGGACATAGTGAAAGAAGACAATACTTTAATGAAACAGATGAAAC
TGTAAATAAAAAAGTAAAAAAAGCCTTCGAACATAACTTAATTCCAATAGTTTGTTGTGGAGAAAGCTTAGAAGAAAGAG
AAGGAAATATAACAGAAAAAGTTTTAGAAGGACAAATAAAAGTTGGATTAAAGGAATTAAGTAAAGAACAAGTAGAAAAA
TTAGTTATAGCATATGAACCAATTTGGGCTATAGGAACAGGAAAAACTGCCACAGATGAGCAAGCAAATGAAACTATAGG
ATATATAAGAACTGTAGTAAAAGCTATGTATGGTGAGAGCGTTGCAGATAAAATAAGAATTCAATACGGTGGATCTGTTA
AGCCTGGCACTATCAAAGCTCAAATGGCTAAAGAAGAGATTGATGGTGCATTAGTAGGTGGTGCAAGCTTAAAAGCAGAA
GATTTTGCGGCTATAGTAAATTATTAA

Upstream 100 bases:

>100_bases
AAAGAACTACCAGGAATAGCAGCTCTTAACGATAAATAAAAGACAGAAAATAAATAGTTACACAGTATTACTTATAAAAT
GTATTGGAGCGTGATTTTAA

Downstream 100 bases:

>100_bases
AAGTGACAAGTGACAAGCTATAAGTTACAAATTGTTTTATATTAGCACTTCTCATTTGTATATTTTTATTTAATGAGATT
TGGAGGTAGAAATGAGTAAA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MRTAIIAGNWKMNKTVKEAVELVKELKPLVKDAKCDVVVCPTYVCLPAVLEEVKGSNIKVGAQNMHFEESGAYTGEIAPK
MLEELGVHYVIIGHSERRQYFNETDETVNKKVKKAFEHNLIPIVCCGESLEEREGNITEKVLEGQIKVGLKELSKEQVEK
LVIAYEPIWAIGTGKTATDEQANETIGYIRTVVKAMYGESVADKIRIQYGGSVKPGTIKAQMAKEEIDGALVGGASLKAE
DFAAIVNY

Sequences:

>Translated_248_residues
MRTAIIAGNWKMNKTVKEAVELVKELKPLVKDAKCDVVVCPTYVCLPAVLEEVKGSNIKVGAQNMHFEESGAYTGEIAPK
MLEELGVHYVIIGHSERRQYFNETDETVNKKVKKAFEHNLIPIVCCGESLEEREGNITEKVLEGQIKVGLKELSKEQVEK
LVIAYEPIWAIGTGKTATDEQANETIGYIRTVVKAMYGESVADKIRIQYGGSVKPGTIKAQMAKEEIDGALVGGASLKAE
DFAAIVNY
>Mature_248_residues
MRTAIIAGNWKMNKTVKEAVELVKELKPLVKDAKCDVVVCPTYVCLPAVLEEVKGSNIKVGAQNMHFEESGAYTGEIAPK
MLEELGVHYVIIGHSERRQYFNETDETVNKKVKKAFEHNLIPIVCCGESLEEREGNITEKVLEGQIKVGLKELSKEQVEK
LVIAYEPIWAIGTGKTATDEQANETIGYIRTVVKAMYGESVADKIRIQYGGSVKPGTIKAQMAKEEIDGALVGGASLKAE
DFAAIVNY

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=249, Percent_Identity=42.9718875502008, Blast_Score=181, Evalue=4e-46,
Organism=Homo sapiens, GI4507645, Length=249, Percent_Identity=42.9718875502008, Blast_Score=181, Evalue=5e-46,
Organism=Escherichia coli, GI1790353, Length=247, Percent_Identity=44.9392712550607, Blast_Score=199, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17536593, Length=246, Percent_Identity=42.2764227642276, Blast_Score=176, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6320255, Length=246, Percent_Identity=39.0243902439024, Blast_Score=172, Evalue=6e-44,
Organism=Drosophila melanogaster, GI28572008, Length=247, Percent_Identity=40.080971659919, Blast_Score=174, Evalue=4e-44,
Organism=Drosophila melanogaster, GI28572006, Length=247, Percent_Identity=40.080971659919, Blast_Score=174, Evalue=4e-44,
Organism=Drosophila melanogaster, GI28572004, Length=247, Percent_Identity=40.080971659919, Blast_Score=174, Evalue=6e-44,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_CLOB1 (A7FQN8)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001382632.1
- ProteinModelPortal:   A7FQN8
- SMR:   A7FQN8
- STRING:   A7FQN8
- GeneID:   5396121
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0269
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   CBOT441770:CLB_0269-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27277; Mature: 27277

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 94-94 ACT_SITE 166-166 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTAIIAGNWKMNKTVKEAVELVKELKPLVKDAKCDVVVCPTYVCLPAVLEEVKGSNIKV
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCEEE
GAQNMHFEESGAYTGEIAPKMLEELGVHYVIIGHSERRQYFNETDETVNKKVKKAFEHNL
CCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECHHHHHHHCHHHHHHHHHHHHHHHCCC
IPIVCCGESLEEREGNITEKVLEGQIKVGLKELSKEQVEKLVIAYEPIWAIGTGKTATDE
EEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEECCCCCCCH
QANETIGYIRTVVKAMYGESVADKIRIQYGGSVKPGTIKAQMAKEEIDGALVGGASLKAE
HHHHHHHHHHHHHHHHHCCHHHHHEEEEECCCCCCCCHHHHHHHHHHCCEEECCCCCCHH
DFAAIVNY
HHHHHHCC
>Mature Secondary Structure
MRTAIIAGNWKMNKTVKEAVELVKELKPLVKDAKCDVVVCPTYVCLPAVLEEVKGSNIKV
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCEEE
GAQNMHFEESGAYTGEIAPKMLEELGVHYVIIGHSERRQYFNETDETVNKKVKKAFEHNL
CCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECHHHHHHHCHHHHHHHHHHHHHHHCCC
IPIVCCGESLEEREGNITEKVLEGQIKVGLKELSKEQVEKLVIAYEPIWAIGTGKTATDE
EEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEECCCCCCCH
QANETIGYIRTVVKAMYGESVADKIRIQYGGSVKPGTIKAQMAKEEIDGALVGGASLKAE
HHHHHHHHHHHHHHHHHCCHHHHHEEEEECCCCCCCCHHHHHHHHHHCCEEECCCCCCHH
DFAAIVNY
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA