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The map label for this gene is rpoN [H]

Identifier: 153935180

GI number: 153935180

Start: 257409

End: 258782

Strand: Direct

Name: rpoN [H]

Synonym: CLC_0280

Alternate gene names: 153935180

Gene position: 257409-258782 (Clockwise)

Preceding gene: 153937491

Following gene: 153936137

Centisome position: 6.84

GC content: 25.91

Gene sequence:

>1374_bases
ATGAATATGAATTTTGGATTGTCCTTAACACAGGAACAAAAGTTAATAATGACACAACAAATGCAGCTATCTATTAAACT
TCTTCAGATGTCTACCTATGAGTTACAACAATATGTTGAAAAGGAAGTTCAAGAAAATCCTGTTTTAGATTCTCAGAATA
TTCATAAAGAAGATAAGGGGATAGAGGATATAAATTATAAAAAATTAATCAAACATTTAGAGGATAATGGAAATGAATAT
GGTAGTTATAGTAGCTACAATAAAGAAGAAGAAACCTCACCTTTTAATTTTATATCTAATGAAAAATCATTAAAAGAGTA
TTTACAAGAACAAATTATAGAATTAAATGAAAACAAATACATAAAGTCCATATGCATATACATAATAGAAAATATAGATG
ATAAAGGGTATTTATCTGTAAGGGATGAGGAAATATCTGAGGAATTAAAAGTACCATTACATTCAATAGAAGAAGCCATA
GATATAGTACAGTCGTTAGAACCAGATGGGATAGGTGCGAGGAATTTAAAAGAATGTTTAAAGATACAATTAAAAAAGAA
ATCCATAAATAATAGTAAAATATGTGAAATTATAGACAATTTCCTTCCTCTAGTAGCAGAAAATAGGTATAATGTTATAT
CTAAAAAACTTAGTATATCAATAAAAAAGGCTCAGGAATATGGCGATGTTATAAAGACTCTTCAACCTAAGCCTTCAAGA
GGATTTTATACTGGTGATGAAGTAAAATACATAGTTCCAGATGCATATATAAAAAAAATAGATAACGAATACTATATAAT
TATGAATGAAGAGCTATTGCCTAAGTTAACAGTAAATAATTTATATAAAGACATAGTTTTAAATGATACTGATGAAAATG
CAGTAGAATTTGTAAAAGAAAAATTAAATAGCGCTATTTTTTTAATTAAAAGTATAGAAAATAGAAAATCTACAATTTAT
AGAGTATTAGAAAAAATTTTAGAAATACAAAGGGATTATTTTGATTTAGGAATTAAGTTCTTAAAGCCTATGACATTAAA
GGAAATAGCCGATAGTTTAGGTATGCATGAGTCTACTATAAGTAGAGCTATAAGAGATAAATATATAAACACTAATAGAG
GGACCATACTTATAAAAGATTTATTTACTACAGGAATTACTGCTAATAATAGTACAGGAGAAGATATTTCTGTAGAATTT
ATAAAAAGAGAAATAAAAGAACTAATAGATAAAGAAGATAAGAAAAAGCCAATATCTGATCAAATAATATGTAATATATT
AAATAATAAAGGCATACAAATATCCAGAAGAACTGTAGCTAAATATAGAGAGGAACTAGGAATACAATCTTCAAAATGTA
GAAAGCGTTTTTAA

Upstream 100 bases:

>100_bases
CTAATGAATATTAAGGTATATAAGTATTTTTAATTCTTAAGTCTGATGTAGTAAAATTTTTTTTATGATATAATTAAAAT
CAAAGGGGGGGTAGGAATTT

Downstream 100 bases:

>100_bases
AAGCATGATTTATAGATTATCATGCTTTTTTTATTTAATAATATGAATTAAATTTTTAAAATTAATAAGGGTATAAAATT
AAAGATATAGAATTAAAAAT

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MNMNFGLSLTQEQKLIMTQQMQLSIKLLQMSTYELQQYVEKEVQENPVLDSQNIHKEDKGIEDINYKKLIKHLEDNGNEY
GSYSSYNKEEETSPFNFISNEKSLKEYLQEQIIELNENKYIKSICIYIIENIDDKGYLSVRDEEISEELKVPLHSIEEAI
DIVQSLEPDGIGARNLKECLKIQLKKKSINNSKICEIIDNFLPLVAENRYNVISKKLSISIKKAQEYGDVIKTLQPKPSR
GFYTGDEVKYIVPDAYIKKIDNEYYIIMNEELLPKLTVNNLYKDIVLNDTDENAVEFVKEKLNSAIFLIKSIENRKSTIY
RVLEKILEIQRDYFDLGIKFLKPMTLKEIADSLGMHESTISRAIRDKYINTNRGTILIKDLFTTGITANNSTGEDISVEF
IKREIKELIDKEDKKKPISDQIICNILNNKGIQISRRTVAKYREELGIQSSKCRKRF

Sequences:

>Translated_457_residues
MNMNFGLSLTQEQKLIMTQQMQLSIKLLQMSTYELQQYVEKEVQENPVLDSQNIHKEDKGIEDINYKKLIKHLEDNGNEY
GSYSSYNKEEETSPFNFISNEKSLKEYLQEQIIELNENKYIKSICIYIIENIDDKGYLSVRDEEISEELKVPLHSIEEAI
DIVQSLEPDGIGARNLKECLKIQLKKKSINNSKICEIIDNFLPLVAENRYNVISKKLSISIKKAQEYGDVIKTLQPKPSR
GFYTGDEVKYIVPDAYIKKIDNEYYIIMNEELLPKLTVNNLYKDIVLNDTDENAVEFVKEKLNSAIFLIKSIENRKSTIY
RVLEKILEIQRDYFDLGIKFLKPMTLKEIADSLGMHESTISRAIRDKYINTNRGTILIKDLFTTGITANNSTGEDISVEF
IKREIKELIDKEDKKKPISDQIICNILNNKGIQISRRTVAKYREELGIQSSKCRKRF
>Mature_457_residues
MNMNFGLSLTQEQKLIMTQQMQLSIKLLQMSTYELQQYVEKEVQENPVLDSQNIHKEDKGIEDINYKKLIKHLEDNGNEY
GSYSSYNKEEETSPFNFISNEKSLKEYLQEQIIELNENKYIKSICIYIIENIDDKGYLSVRDEEISEELKVPLHSIEEAI
DIVQSLEPDGIGARNLKECLKIQLKKKSINNSKICEIIDNFLPLVAENRYNVISKKLSISIKKAQEYGDVIKTLQPKPSR
GFYTGDEVKYIVPDAYIKKIDNEYYIIMNEELLPKLTVNNLYKDIVLNDTDENAVEFVKEKLNSAIFLIKSIENRKSTIY
RVLEKILEIQRDYFDLGIKFLKPMTLKEIADSLGMHESTISRAIRDKYINTNRGTILIKDLFTTGITANNSTGEDISVEF
IKREIKELIDKEDKKKPISDQIICNILNNKGIQISRRTVAKYREELGIQSSKCRKRF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=481, Percent_Identity=30.5613305613306, Blast_Score=243, Evalue=2e-65,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 53125; Mature: 53125

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMNFGLSLTQEQKLIMTQQMQLSIKLLQMSTYELQQYVEKEVQENPVLDSQNIHKEDKG
CCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCC
IEDINYKKLIKHLEDNGNEYGSYSSYNKEEETSPFNFISNEKSLKEYLQEQIIELNENKY
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCHH
IKSICIYIIENIDDKGYLSVRDEEISEELKVPLHSIEEAIDIVQSLEPDGIGARNLKECL
HHHHHHHHHHCCCCCCCEEECHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
KIQLKKKSINNSKICEIIDNFLPLVAENRYNVISKKLSISIKKAQEYGDVIKTLQPKPSR
HHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GFYTGDEVKYIVPDAYIKKIDNEYYIIMNEELLPKLTVNNLYKDIVLNDTDENAVEFVKE
CCCCCCCCEEECCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
KLNSAIFLIKSIENRKSTIYRVLEKILEIQRDYFDLGIKFLKPMTLKEIADSLGMHESTI
HHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
SRAIRDKYINTNRGTILIKDLFTTGITANNSTGEDISVEFIKREIKELIDKEDKKKPISD
HHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHH
QIICNILNNKGIQISRRTVAKYREELGIQSSKCRKRF
HHHHHHHCCCCCEEHHHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure
MNMNFGLSLTQEQKLIMTQQMQLSIKLLQMSTYELQQYVEKEVQENPVLDSQNIHKEDKG
CCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCC
IEDINYKKLIKHLEDNGNEYGSYSSYNKEEETSPFNFISNEKSLKEYLQEQIIELNENKY
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCHH
IKSICIYIIENIDDKGYLSVRDEEISEELKVPLHSIEEAIDIVQSLEPDGIGARNLKECL
HHHHHHHHHHCCCCCCCEEECHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
KIQLKKKSINNSKICEIIDNFLPLVAENRYNVISKKLSISIKKAQEYGDVIKTLQPKPSR
HHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GFYTGDEVKYIVPDAYIKKIDNEYYIIMNEELLPKLTVNNLYKDIVLNDTDENAVEFVKE
CCCCCCCCEEECCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
KLNSAIFLIKSIENRKSTIYRVLEKILEIQRDYFDLGIKFLKPMTLKEIADSLGMHESTI
HHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
SRAIRDKYINTNRGTILIKDLFTTGITANNSTGEDISVEFIKREIKELIDKEDKKKPISD
HHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHH
QIICNILNNKGIQISRRTVAKYREELGIQSSKCRKRF
HHHHHHHCCCCCEEHHHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550525 [H]