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The map label for this gene is ispF

Identifier: 153934984

GI number: 153934984

Start: 91231

End: 91698

Strand: Reverse

Name: ispF

Synonym: CLC_0114

Alternate gene names: 153934984

Gene position: 91698-91231 (Counterclockwise)

Preceding gene: 153936084

Following gene: 153934492

Centisome position: 2.44

GC content: 28.85

Gene sequence:

>468_bases
ATGAGAATAGGATTGGGATACGATGTACATAAACTAGTTGAAAATAGACCACTAATAATAGGTGGTGTTACAATTCCCCA
TGATAAAGGTTTACTAGGTCATTCTGATGCAGATGTATTGGTCCATGCAATTATGGACGCTTTATTAGGAGCCGCGGCAT
TAGGTGATATAGGTAAGCATTTTCCTGATTCAGATAAAAATTTTAAAAATATATCAAGTCTATTACTTTTATCTAAAGTT
AAAGATTTAATAAACAAAGAAGGTTATAAAATTGTAAATATAGACTGTACTATAATAGCTCAAAAACCTAAAATGCTTTA
TCATATAGATGCTATGAAAAAAAATATTTGTAAGTGTTTAAAATTAGATAATAATATGTTAAATATAAAAGCTACTACAG
AAGAAGGTTTAGGTTTTACAGGTAAAGAAGAAGGAATCTCCGCTAATGCTATTTGTCTTTTGGATTAA

Upstream 100 bases:

>100_bases
AAATGATAATAAAAATATATCTTAAGGTGCAACTTTGTTGCACTTTTTTTTAAAGTATATATCATTATATATATAATATT
AAATTATTGGAGGTTTTTTA

Downstream 100 bases:

>100_bases
GACTATAAATTTAAAAATATTTAATTTACCAAAAATATAAGAGTTCACCTTAATTTATAGGTGAACTCTTATTTGAATTT
ATATTTTATTTAAGATCTTT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MRIGLGYDVHKLVENRPLIIGGVTIPHDKGLLGHSDADVLVHAIMDALLGAAALGDIGKHFPDSDKNFKNISSLLLLSKV
KDLINKEGYKIVNIDCTIIAQKPKMLYHIDAMKKNICKCLKLDNNMLNIKATTEEGLGFTGKEEGISANAICLLD

Sequences:

>Translated_155_residues
MRIGLGYDVHKLVENRPLIIGGVTIPHDKGLLGHSDADVLVHAIMDALLGAAALGDIGKHFPDSDKNFKNISSLLLLSKV
KDLINKEGYKIVNIDCTIIAQKPKMLYHIDAMKKNICKCLKLDNNMLNIKATTEEGLGFTGKEEGISANAICLLD
>Mature_155_residues
MRIGLGYDVHKLVENRPLIIGGVTIPHDKGLLGHSDADVLVHAIMDALLGAAALGDIGKHFPDSDKNFKNISSLLLLSKV
KDLINKEGYKIVNIDCTIIAQKPKMLYHIDAMKKNICKCLKLDNNMLNIKATTEEGLGFTGKEEGISANAICLLD

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=55.8441558441558, Blast_Score=153, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_CLOB1 (A7FQ95)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001382473.1
- ProteinModelPortal:   A7FQ95
- SMR:   A7FQ95
- STRING:   A7FQ95
- GeneID:   5397637
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0102
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   CBOT441770:CLB_0102-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16863; Mature: 16863

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGLGYDVHKLVENRPLIIGGVTIPHDKGLLGHSDADVLVHAIMDALLGAAALGDIGKH
CCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
FPDSDKNFKNISSLLLLSKVKDLINKEGYKIVNIDCTIIAQKPKMLYHIDAMKKNICKCL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCEEEEHHHHHHHHHHHH
KLDNNMLNIKATTEEGLGFTGKEEGISANAICLLD
HCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MRIGLGYDVHKLVENRPLIIGGVTIPHDKGLLGHSDADVLVHAIMDALLGAAALGDIGKH
CCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
FPDSDKNFKNISSLLLLSKVKDLINKEGYKIVNIDCTIIAQKPKMLYHIDAMKKNICKCL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCEEEEHHHHHHHHHHHH
KLDNNMLNIKATTEEGLGFTGKEEGISANAICLLD
HCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA