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The map label for this gene is rnfE [H]

Identifier: 153934982

GI number: 153934982

Start: 425043

End: 425645

Strand: Direct

Name: rnfE [H]

Synonym: CLC_0429

Alternate gene names: 153934982

Gene position: 425043-425645 (Clockwise)

Preceding gene: 153937850

Following gene: 153935903

Centisome position: 11.3

GC content: 31.84

Gene sequence:

>603_bases
ATGATGAGCGTTTTAGGAGAAAGATTAAAAAATGGAATTATAGACGAAAATGTTACCTTTGTCCAAGTTTTAGCAATGTG
TCCTACTTTGGCAGTAACATCAAGTGCTGAGAATGGTATAGGTATGGGTATTGCATCTACAGTAGTTCTTATGGGTTCAA
ACTTAGTTATATCATTACTTAGAAAAGTAATACCAGATAAGATTCGTATACCAGCATTTATAGTAGTAATAGCAGGATTT
GTTACATTGCTTCAATTTTTAATGCAAGGTTTTGTACCAGATTTATATACAAAATTAGGTATATTTATACCACTTATAGT
TGTTAACTGTGTTATCCTAGGAAGAGCGGAAGCTTATGCATCAAAATATGGTCCAGTATCTTCAATATTTGATGGATTAG
GTCAAGGTCTAGGATTTACATTATCATTAGGAGTAATAGGAATGATAAGAGAATTATTAGGAGCAGGCCAATTATTTGGA
CATCAAATAATGCCATCTTCATTCCAACCAGCTTTAATAATGATACTTGCGCCAGGAGCATTCTTTACACTAGGAATATT
AATGGCAATAATAAACAATATGAAATTAAAGAAAGCTAAATAA

Upstream 100 bases:

>100_bases
GTAGATGGTAAATCAGGGGCTACAACAAGCTTTAACACAGAGGACACAAAGTTAAATAAGTATGTTGTAAACTATGAAAA
AATGAATTAAAGGGGGATAA

Downstream 100 bases:

>100_bases
CCAAGAGAAGGAATTTAAAATATCCTTATTAAGAAAGAAGGGTGAAAAGTATGAAAATATTTGCTTTGATTATATCAGCG
TTATTTGTTAATAACTTCGT

Product: electron transport complex RsxE subunit

Products: NA

Alternate protein names: Nitrogen fixation protein rnfE [H]

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MMSVLGERLKNGIIDENVTFVQVLAMCPTLAVTSSAENGIGMGIASTVVLMGSNLVISLLRKVIPDKIRIPAFIVVIAGF
VTLLQFLMQGFVPDLYTKLGIFIPLIVVNCVILGRAEAYASKYGPVSSIFDGLGQGLGFTLSLGVIGMIRELLGAGQLFG
HQIMPSSFQPALIMILAPGAFFTLGILMAIINNMKLKKAK

Sequences:

>Translated_200_residues
MMSVLGERLKNGIIDENVTFVQVLAMCPTLAVTSSAENGIGMGIASTVVLMGSNLVISLLRKVIPDKIRIPAFIVVIAGF
VTLLQFLMQGFVPDLYTKLGIFIPLIVVNCVILGRAEAYASKYGPVSSIFDGLGQGLGFTLSLGVIGMIRELLGAGQLFG
HQIMPSSFQPALIMILAPGAFFTLGILMAIINNMKLKKAK
>Mature_200_residues
MMSVLGERLKNGIIDENVTFVQVLAMCPTLAVTSSAENGIGMGIASTVVLMGSNLVISLLRKVIPDKIRIPAFIVVIAGF
VTLLQFLMQGFVPDLYTKLGIFIPLIVVNCVILGRAEAYASKYGPVSSIFDGLGQGLGFTLSLGVIGMIRELLGAGQLFG
HQIMPSSFQPALIMILAPGAFFTLGILMAIINNMKLKKAK

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG4660

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfE

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787919, Length=201, Percent_Identity=42.7860696517413, Blast_Score=141, Evalue=3e-35,
Organism=Escherichia coli, GI1787914, Length=173, Percent_Identity=30.0578034682081, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR010968 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 21288; Mature: 21288

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSVLGERLKNGIIDENVTFVQVLAMCPTLAVTSSAENGIGMGIASTVVLMGSNLVISLL
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHH
RKVIPDKIRIPAFIVVIAGFVTLLQFLMQGFVPDLYTKLGIFIPLIVVNCVILGRAEAYA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKYGPVSSIFDGLGQGLGFTLSLGVIGMIRELLGAGQLFGHQIMPSSFQPALIMILAPGA
HHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHH
FFTLGILMAIINNMKLKKAK
HHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMSVLGERLKNGIIDENVTFVQVLAMCPTLAVTSSAENGIGMGIASTVVLMGSNLVISLL
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHH
RKVIPDKIRIPAFIVVIAGFVTLLQFLMQGFVPDLYTKLGIFIPLIVVNCVILGRAEAYA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKYGPVSSIFDGLGQGLGFTLSLGVIGMIRELLGAGQLFGHQIMPSSFQPALIMILAPGA
HHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHH
FFTLGILMAIINNMKLKKAK
HHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8264535; 9492268 [H]