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The map label for this gene is epsE [H]

Identifier: 153934925

GI number: 153934925

Start: 1957820

End: 1959349

Strand: Reverse

Name: epsE [H]

Synonym: CLC_1853

Alternate gene names: 153934925

Gene position: 1959349-1957820 (Counterclockwise)

Preceding gene: 153937818

Following gene: 153934559

Centisome position: 52.1

GC content: 26.47

Gene sequence:

>1530_bases
ATGAGCTTTTCTATGAATTATTTAGATTTAAAGGAAACTGTACAAAACTCTGAAGAATTAAAAGGTGGAAAGCAGTGTGA
AAATATAGACTTTAGTACTTTAAAAATAGAATCTTCTCTGCTAAAATTGCTGCCAGAGCATATTTGCAGGAAATATAATA
TATTACCCTTAAAAATAATTGGCGAACAATTGTATATAGCATCTTCAAATTACTTAAAAGAAGATGCTTTATATAAAATA
TCCTTTATTACGGGTAAAAATATAAAAATAGTTCTTTGTAATAAAGAAGATATTTTAGGGGCTATAAAGAGATTTTATAC
AAACTATGAAGGAAAATATAATGTTAAAGGAATAAAGGAAGAAAAAACTATATCCTATGAAGAAAATAAAGAAAAAAATT
TAGAAGGCCCTATAATAAAGTTAACAGATTCTATAATAGAAGAGGCTGTTTGGAGAAAGGCTAGTGATATACATATAGAA
CCATTCAAAGATTTTGCTTTAATAAGATTTAGAATAGATGGCATTTTAATAGAATTCAAAAAAATATCCAAAAAAATTTA
CACTTCTATTTGCACTCGTATAAAAATAATGAGTTATATGAACATAGCAGAAAAAAATATTCCCCAGGATGGTAAAATAC
AAAACAATTATAGAGAAAATAATGACTTTAGAGTATCTACATTGCCCACAGTATACGGTGAAAAATTAGTGATAAGAATA
CTATATAAAAATGAAAAAATAGCAGATTTAAAATCTTTAGGATTTTTAAAAGAAGATAGAAAAAATATAGAAAAAATGCT
TAAAAAGCCTAATGGATTAATTTTGATTAGTGGTCCGACGGGAAGTGGTAAATCTACAACTTTATATTCTATGCTTCAAT
ATATAAACAATAAAGAAAAAAACATAATAACCATTGAAGAGCCAGTAGAATATACTATAGATGGTATAAACCAAGTTAAT
GTAGATTATAAAAGGGAACTTACTTTTCTAAAAGGTTTAAAATCAATACTTAGACAGGACCCGGATATAATAATGGTAGG
AGAAATTAGAGATGAAGAAACAGCTAAAGTAGCGGTAAGAGCTTCTATAACAGGACATTTAGTTTTATCTACTCTACATA
CTAATGGAGCCTTAGAATCTATAATAAGGCTTTTGGATATGAAAATAGAACCCTATATTCTAAGTGATGCCTTAAGAGGA
ATTATTTCACAAAGATTGGTGAGAAAGATATGTCCTTATTGCAAAGAAGCATATATACCTTCAACGGAGGAAAAGAACTT
CGTAAATTTAAAAGAAGAGTTTTTACTATATAAGGGTAAAGGATGCAATAAATGCAATAATACAGGTTATATAGGTAGAA
GCGTTCTTTATGAATATATAAATATTGATAAAGAAAAAAAGAACTTAATTAAAAACATAGTAAAAAACAATAATGAAGAA
ATTTTTTTAGAAAATAGCTTAAAAGAAAATGTTAATAAAGCATTAATACAAGGAAGGACATCCTTTAGTGAAATCCAAAG
AATAATATAA

Upstream 100 bases:

>100_bases
TTTAAAACATAGTCAATATATAAAATAAAGTGTTTACTTTTTATATATGTGTCTATAATATAGATAAATTTTCAAATTTT
AATATAGGAGAGAATTAAAT

Downstream 100 bases:

>100_bases
ACAGAATCTTTAGTAACTAAACATCCTTACTAAAGAAGCAGCTAGATTGCTTTAAGATTCATTTTCAGTATATATTTCTA
AGAAAATGTCTTTTAAACTT

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MSFSMNYLDLKETVQNSEELKGGKQCENIDFSTLKIESSLLKLLPEHICRKYNILPLKIIGEQLYIASSNYLKEDALYKI
SFITGKNIKIVLCNKEDILGAIKRFYTNYEGKYNVKGIKEEKTISYEENKEKNLEGPIIKLTDSIIEEAVWRKASDIHIE
PFKDFALIRFRIDGILIEFKKISKKIYTSICTRIKIMSYMNIAEKNIPQDGKIQNNYRENNDFRVSTLPTVYGEKLVIRI
LYKNEKIADLKSLGFLKEDRKNIEKMLKKPNGLILISGPTGSGKSTTLYSMLQYINNKEKNIITIEEPVEYTIDGINQVN
VDYKRELTFLKGLKSILRQDPDIIMVGEIRDEETAKVAVRASITGHLVLSTLHTNGALESIIRLLDMKIEPYILSDALRG
IISQRLVRKICPYCKEAYIPSTEEKNFVNLKEEFLLYKGKGCNKCNNTGYIGRSVLYEYINIDKEKKNLIKNIVKNNNEE
IFLENSLKENVNKALIQGRTSFSEIQRII

Sequences:

>Translated_509_residues
MSFSMNYLDLKETVQNSEELKGGKQCENIDFSTLKIESSLLKLLPEHICRKYNILPLKIIGEQLYIASSNYLKEDALYKI
SFITGKNIKIVLCNKEDILGAIKRFYTNYEGKYNVKGIKEEKTISYEENKEKNLEGPIIKLTDSIIEEAVWRKASDIHIE
PFKDFALIRFRIDGILIEFKKISKKIYTSICTRIKIMSYMNIAEKNIPQDGKIQNNYRENNDFRVSTLPTVYGEKLVIRI
LYKNEKIADLKSLGFLKEDRKNIEKMLKKPNGLILISGPTGSGKSTTLYSMLQYINNKEKNIITIEEPVEYTIDGINQVN
VDYKRELTFLKGLKSILRQDPDIIMVGEIRDEETAKVAVRASITGHLVLSTLHTNGALESIIRLLDMKIEPYILSDALRG
IISQRLVRKICPYCKEAYIPSTEEKNFVNLKEEFLLYKGKGCNKCNNTGYIGRSVLYEYINIDKEKKNLIKNIVKNNNEE
IFLENSLKENVNKALIQGRTSFSEIQRII
>Mature_508_residues
SFSMNYLDLKETVQNSEELKGGKQCENIDFSTLKIESSLLKLLPEHICRKYNILPLKIIGEQLYIASSNYLKEDALYKIS
FITGKNIKIVLCNKEDILGAIKRFYTNYEGKYNVKGIKEEKTISYEENKEKNLEGPIIKLTDSIIEEAVWRKASDIHIEP
FKDFALIRFRIDGILIEFKKISKKIYTSICTRIKIMSYMNIAEKNIPQDGKIQNNYRENNDFRVSTLPTVYGEKLVIRIL
YKNEKIADLKSLGFLKEDRKNIEKMLKKPNGLILISGPTGSGKSTTLYSMLQYINNKEKNIITIEEPVEYTIDGINQVNV
DYKRELTFLKGLKSILRQDPDIIMVGEIRDEETAKVAVRASITGHLVLSTLHTNGALESIIRLLDMKIEPYILSDALRGI
ISQRLVRKICPYCKEAYIPSTEEKNFVNLKEEFLLYKGKGCNKCNNTGYIGRSVLYEYINIDKEKKNLIKNIVKNNNEEI
FLENSLKENVNKALIQGRTSFSEIQRII

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=384, Percent_Identity=44.0104166666667, Blast_Score=348, Evalue=4e-97,
Organism=Escherichia coli, GI1786296, Length=476, Percent_Identity=34.2436974789916, Blast_Score=297, Evalue=9e-82,
Organism=Escherichia coli, GI87082188, Length=200, Percent_Identity=40.5, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 58689; Mature: 58558

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSMNYLDLKETVQNSEELKGGKQCENIDFSTLKIESSLLKLLPEHICRKYNILPLKII
CCCCCCHHHHHHHHCCHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEEE
GEQLYIASSNYLKEDALYKISFITGKNIKIVLCNKEDILGAIKRFYTNYEGKYNVKGIKE
CCEEEEECCCCCCCCCEEEEEEEECCCEEEEEECCHHHHHHHHHHHCCCCCEEECCCCCC
EKTISYEENKEKNLEGPIIKLTDSIIEEAVWRKASDIHIEPFKDFALIRFRIDGILIEFK
CCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCEEEHHH
KISKKIYTSICTRIKIMSYMNIAEKNIPQDGKIQNNYRENNDFRVSTLPTVYGEKLVIRI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHCCEEEEEE
LYKNEKIADLKSLGFLKEDRKNIEKMLKKPNGLILISGPTGSGKSTTLYSMLQYINNKEK
EECCCCHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCC
NIITIEEPVEYTIDGINQVNVDYKRELTFLKGLKSILRQDPDIIMVGEIRDEETAKVAVR
CEEEECCCCHHHHCCCEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEE
ASITGHLVLSTLHTNGALESIIRLLDMKIEPYILSDALRGIISQRLVRKICPYCKEAYIP
EECCEEEEEEEHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
STEEKNFVNLKEEFLLYKGKGCNKCNNTGYIGRSVLYEYINIDKEKKNLIKNIVKNNNEE
CCCCCCCCCCHHHHEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCE
IFLENSLKENVNKALIQGRTSFSEIQRII
EEEECHHHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
SFSMNYLDLKETVQNSEELKGGKQCENIDFSTLKIESSLLKLLPEHICRKYNILPLKII
CCCCCHHHHHHHHCCHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEEE
GEQLYIASSNYLKEDALYKISFITGKNIKIVLCNKEDILGAIKRFYTNYEGKYNVKGIKE
CCEEEEECCCCCCCCCEEEEEEEECCCEEEEEECCHHHHHHHHHHHCCCCCEEECCCCCC
EKTISYEENKEKNLEGPIIKLTDSIIEEAVWRKASDIHIEPFKDFALIRFRIDGILIEFK
CCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCEEEHHH
KISKKIYTSICTRIKIMSYMNIAEKNIPQDGKIQNNYRENNDFRVSTLPTVYGEKLVIRI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHCCEEEEEE
LYKNEKIADLKSLGFLKEDRKNIEKMLKKPNGLILISGPTGSGKSTTLYSMLQYINNKEK
EECCCCHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCC
NIITIEEPVEYTIDGINQVNVDYKRELTFLKGLKSILRQDPDIIMVGEIRDEETAKVAVR
CEEEECCCCHHHHCCCEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEE
ASITGHLVLSTLHTNGALESIIRLLDMKIEPYILSDALRGIISQRLVRKICPYCKEAYIP
EECCEEEEEEEHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
STEEKNFVNLKEEFLLYKGKGCNKCNNTGYIGRSVLYEYINIDKEKKNLIKNIVKNNNEE
CCCCCCCCCCHHHHEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCE
IFLENSLKENVNKALIQGRTSFSEIQRII
EEEECHHHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]