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The map label for this gene is panB

Identifier: 153932728

GI number: 153932728

Start: 471310

End: 472137

Strand: Reverse

Name: panB

Synonym: CLB_0458

Alternate gene names: 153932728

Gene position: 472137-471310 (Counterclockwise)

Preceding gene: 153933719

Following gene: 153933524

Centisome position: 12.22

GC content: 33.09

Gene sequence:

>828_bases
ATGCGTAATACTGTATCAACTTTTCAAGAATTAAAAAACAAAGGTGAAAAAATAACTATGTTAACTGCCTATGATTATTC
TATGGCAAAGCTTATAGACTCCTCTGGAATCAATGGAATACTAGTTGGGGACTCTTTAGGAATGGTATGCTTAGGATATG
AAAACACACTAAGTGTAACTATGGAAGATATGCTCCATCATACTAAGGCTGTAGTAAGAGGAACTTCTAACGCTTTAGTT
GTGGGAGATATGCCCTTTATGTCTTACCAAACTTCTATTTATGATGCGGTTTATAATGCAGGAAGATTTATAAAAGAAGC
TGGCGCTCACGCTGTAAAATTAGAGGGTGGTGCCACTGTAGCTGAAGAAATTAAAGCTATAGTTAAAGCTCAAATCCCTG
TAATGGGACATATAGGTTTAACCCCTCAATCTGTAAATATGTTTGGAGGATTCAAAGTTCAGGGGAAAAACGAAAAAGTA
GCAAAGAAACTAATAGAAGATGCTAAAATTTTAGAAGAGGCGGGAGCTTTTTCTATAGTTCTAGAATGTATCCCTGAAAA
ATTATCAAAGATAATATCAGAATCCATCTCCATCCCAACCATAGGCATAGGTGCAGGAAAATATTGTGATGGTCAAATAC
TAGTTTATCAAGATATGCTTTCTATGTTTTCAGATTTCAAACCTAAATTTGTTAAGTCCTTTGGAAATATAGGAGAATCT
ATAAAAGATGGTGTTTCACAATATATAAAAGAAGTTAAAGAAGTTAAGTTTCCTGAAGAAAAACATGCTTTTAAAATAGA
TGATGATGTAATAAATAAATTATATTAG

Upstream 100 bases:

>100_bases
TTTATAAAAACAATTCGGATGCACATTGGTATTCTATCCATCCTAATAAATTTTTACAAAATTATTTACAATATAATATT
AATATTTTAGGAGGGATACG

Downstream 100 bases:

>100_bases
TTTTTAAATAACCAGCAGAGGGAGGGTAAAGGCTGATTTTCTCAGCTGAACAATATGAATATAGTTAATACAATAAAAGA
TGTTAGATTAATCATAAAAA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MRNTVSTFQELKNKGEKITMLTAYDYSMAKLIDSSGINGILVGDSLGMVCLGYENTLSVTMEDMLHHTKAVVRGTSNALV
VGDMPFMSYQTSIYDAVYNAGRFIKEAGAHAVKLEGGATVAEEIKAIVKAQIPVMGHIGLTPQSVNMFGGFKVQGKNEKV
AKKLIEDAKILEEAGAFSIVLECIPEKLSKIISESISIPTIGIGAGKYCDGQILVYQDMLSMFSDFKPKFVKSFGNIGES
IKDGVSQYIKEVKEVKFPEEKHAFKIDDDVINKLY

Sequences:

>Translated_275_residues
MRNTVSTFQELKNKGEKITMLTAYDYSMAKLIDSSGINGILVGDSLGMVCLGYENTLSVTMEDMLHHTKAVVRGTSNALV
VGDMPFMSYQTSIYDAVYNAGRFIKEAGAHAVKLEGGATVAEEIKAIVKAQIPVMGHIGLTPQSVNMFGGFKVQGKNEKV
AKKLIEDAKILEEAGAFSIVLECIPEKLSKIISESISIPTIGIGAGKYCDGQILVYQDMLSMFSDFKPKFVKSFGNIGES
IKDGVSQYIKEVKEVKFPEEKHAFKIDDDVINKLY
>Mature_275_residues
MRNTVSTFQELKNKGEKITMLTAYDYSMAKLIDSSGINGILVGDSLGMVCLGYENTLSVTMEDMLHHTKAVVRGTSNALV
VGDMPFMSYQTSIYDAVYNAGRFIKEAGAHAVKLEGGATVAEEIKAIVKAQIPVMGHIGLTPQSVNMFGGFKVQGKNEKV
AKKLIEDAKILEEAGAFSIVLECIPEKLSKIISESISIPTIGIGAGKYCDGQILVYQDMLSMFSDFKPKFVKSFGNIGES
IKDGVSQYIKEVKEVKFPEEKHAFKIDDDVINKLY

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=262, Percent_Identity=46.1832061068702, Blast_Score=242, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6319653, Length=280, Percent_Identity=37.8571428571429, Blast_Score=204, Evalue=1e-53,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_CLOB1 (A7FR60)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001382809.1
- ProteinModelPortal:   A7FR60
- SMR:   A7FR60
- STRING:   A7FR60
- GeneID:   5394821
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0458
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   CBOT441770:CLB_0458-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 30073; Mature: 30073

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: ACT_SITE 182-182 BINDING 83-83 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNTVSTFQELKNKGEKITMLTAYDYSMAKLIDSSGINGILVGDSLGMVCLGYENTLSVT
CCCHHHHHHHHHCCCCEEEEEEECCHHHHHHHHCCCCCEEEEECCCCEEEECCCCHHHHH
MEDMLHHTKAVVRGTSNALVVGDMPFMSYQTSIYDAVYNAGRFIKEAGAHAVKLEGGATV
HHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHH
AEEIKAIVKAQIPVMGHIGLTPQSVNMFGGFKVQGKNEKVAKKLIEDAKILEEAGAFSIV
HHHHHHHHHHCCCEEEECCCCCCCCHHCCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHH
LECIPEKLSKIISESISIPTIGIGAGKYCDGQILVYQDMLSMFSDFKPKFVKSFGNIGES
HHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHH
IKDGVSQYIKEVKEVKFPEEKHAFKIDDDVINKLY
HHHHHHHHHHHHHHCCCCCHHCCEECCHHHHHHCC
>Mature Secondary Structure
MRNTVSTFQELKNKGEKITMLTAYDYSMAKLIDSSGINGILVGDSLGMVCLGYENTLSVT
CCCHHHHHHHHHCCCCEEEEEEECCHHHHHHHHCCCCCEEEEECCCCEEEECCCCHHHHH
MEDMLHHTKAVVRGTSNALVVGDMPFMSYQTSIYDAVYNAGRFIKEAGAHAVKLEGGATV
HHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHH
AEEIKAIVKAQIPVMGHIGLTPQSVNMFGGFKVQGKNEKVAKKLIEDAKILEEAGAFSIV
HHHHHHHHHHCCCEEEECCCCCCCCHHCCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHH
LECIPEKLSKIISESISIPTIGIGAGKYCDGQILVYQDMLSMFSDFKPKFVKSFGNIGES
HHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHH
IKDGVSQYIKEVKEVKFPEEKHAFKIDDDVINKLY
HHHHHHHHHHHHHHCCCCCHHCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA