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The map label for this gene is rfbJ [H]

Identifier: 153932473

GI number: 153932473

Start: 2776860

End: 2777780

Strand: Reverse

Name: rfbJ [H]

Synonym: CLB_2655

Alternate gene names: 153932473

Gene position: 2777780-2776860 (Counterclockwise)

Preceding gene: 153933465

Following gene: 153932449

Centisome position: 71.9

GC content: 27.47

Gene sequence:

>921_bases
ATGAAAAAAGTATTGGTAACAGGAGCTAGTGGATTTATAGGATATAAAACTTTGAATTTCTTAATAAACAAAGGGTATGA
AGTACATGCATTAACAACAAGAGGTATTAATATTTCCGATAATAGCATTGTTAAATGGTACAAAATAAATTTATTAAACA
ATGACGAAACAGAAAAAATTGTACAAGTTATAAAACCAAATTATATTTTACACTTTGCATGGTATGCTGTACCAGGAAAG
TATAGTAGTGCAAAGGAAAATTTAGATTGGGTTCAAAGTAGCATTCAATTACTGAAAAGCTTTAAAAAATATGGAGGAGA
GAGATTTATATTTGCAGGTACTTGTTTTCAATATGATTTAGGTTATGGACTTATGGATGAAAATGTAACTCCATCTATTC
CAAATTCATTATATGGTATATGCAAAAATAGTTTTGAAAATATGGCTAGAGAATATTGTTGTAAAAATGGTATGTCTTTT
GCTTCAGGAAGAATTTTTTATATTTATGGAGAAAGAGAAAATAAGAATAGAATAATTCCGTATGTAATAAATAGCTTGCT
TAATGGCAAAGTAGCTAACTGTTCTCATGGAAATCAAATAAGGGATTTTATGCATGTAGATGATGTAGCAAATGCATTTG
TAGAAATTTTAGATAGTAGTATAGATGGTGTAATAAACATAGGTTCTGGACAAGCTATAAATATAAAAGAAATTTTGTTT
AAAGTAGGTGAAAAATTAAATAAAAAAGAACTAATAAATCTAGGAGCTATAAAAACTGCATCTAATGAGCCAAAAATGAT
AGTTGCAAATAATGATAGATTAAAAAATGAAACTAATTGGAGTCAATGCTATAGTTTAGAACGAGGAATTGAAAAAACTA
TTAATTGGTGGAAGGAGATGAAGATAGAAAATGGAATATAG

Upstream 100 bases:

>100_bases
GGGGAATTAGGTGGGATGATTTAACCTTTAGCGTAGATTGGCCGGTACGGGGGGATGTAATTATTTCTTATAAGGATAAA
AATTGGGGAGATTTTATAGT

Downstream 100 bases:

>100_bases
TTGTCCTATATGTAATTCTAAAAAAATTGTTGGATTTTTACAAAGAACCGATGTGCCAGTACATCAGAATTATATTGTTA
AAACTAAAGAGGAAGCCATT

Product: NAD-dependent epimerase/dehydratase family protein

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKKVLVTGASGFIGYKTLNFLINKGYEVHALTTRGINISDNSIVKWYKINLLNNDETEKIVQVIKPNYILHFAWYAVPGK
YSSAKENLDWVQSSIQLLKSFKKYGGERFIFAGTCFQYDLGYGLMDENVTPSIPNSLYGICKNSFENMAREYCCKNGMSF
ASGRIFYIYGERENKNRIIPYVINSLLNGKVANCSHGNQIRDFMHVDDVANAFVEILDSSIDGVINIGSGQAINIKEILF
KVGEKLNKKELINLGAIKTASNEPKMIVANNDRLKNETNWSQCYSLERGIEKTINWWKEMKIENGI

Sequences:

>Translated_306_residues
MKKVLVTGASGFIGYKTLNFLINKGYEVHALTTRGINISDNSIVKWYKINLLNNDETEKIVQVIKPNYILHFAWYAVPGK
YSSAKENLDWVQSSIQLLKSFKKYGGERFIFAGTCFQYDLGYGLMDENVTPSIPNSLYGICKNSFENMAREYCCKNGMSF
ASGRIFYIYGERENKNRIIPYVINSLLNGKVANCSHGNQIRDFMHVDDVANAFVEILDSSIDGVINIGSGQAINIKEILF
KVGEKLNKKELINLGAIKTASNEPKMIVANNDRLKNETNWSQCYSLERGIEKTINWWKEMKIENGI
>Mature_306_residues
MKKVLVTGASGFIGYKTLNFLINKGYEVHALTTRGINISDNSIVKWYKINLLNNDETEKIVQVIKPNYILHFAWYAVPGK
YSSAKENLDWVQSSIQLLKSFKKYGGERFIFAGTCFQYDLGYGLMDENVTPSIPNSLYGICKNSFENMAREYCCKNGMSF
ASGRIFYIYGERENKNRIIPYVINSLLNGKVANCSHGNQIRDFMHVDDVANAFVEILDSSIDGVINIGSGQAINIKEILF
KVGEKLNKKELINLGAIKTASNEPKMIVANNDRLKNETNWSQCYSLERGIEKTINWWKEMKIENGI

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI42516563, Length=319, Percent_Identity=24.4514106583072, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 34740; Mature: 34740

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLVTGASGFIGYKTLNFLINKGYEVHALTTRGINISDNSIVKWYKINLLNNDETEKI
CCEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEEEEEEEEECCCHHHHH
VQVIKPNYILHFAWYAVPGKYSSAKENLDWVQSSIQLLKSFKKYGGERFIFAGTCFQYDL
HHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEC
GYGLMDENVTPSIPNSLYGICKNSFENMAREYCCKNGMSFASGRIFYIYGERENKNRIIP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHH
YVINSLLNGKVANCSHGNQIRDFMHVDDVANAFVEILDSSIDGVINIGSGQAINIKEILF
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEECHHHHHH
KVGEKLNKKELINLGAIKTASNEPKMIVANNDRLKNETNWSQCYSLERGIEKTINWWKEM
HHHHCCCHHHHHCCCCEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KIENGI
HCCCCC
>Mature Secondary Structure
MKKVLVTGASGFIGYKTLNFLINKGYEVHALTTRGINISDNSIVKWYKINLLNNDETEKI
CCEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEEEEEEEEECCCHHHHH
VQVIKPNYILHFAWYAVPGKYSSAKENLDWVQSSIQLLKSFKKYGGERFIFAGTCFQYDL
HHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEC
GYGLMDENVTPSIPNSLYGICKNSFENMAREYCCKNGMSFASGRIFYIYGERENKNRIIP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHH
YVINSLLNGKVANCSHGNQIRDFMHVDDVANAFVEILDSSIDGVINIGSGQAINIKEILF
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEECHHHHHH
KVGEKLNKKELINLGAIKTASNEPKMIVANNDRLKNETNWSQCYSLERGIEKTINWWKEM
HHHHCCCHHHHHCCCCEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KIENGI
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379320 [H]