Click here to switch to the map view.

The map label for this gene is bbvIM [H]

Identifier: 153932387

GI number: 153932387

Start: 2962770

End: 2963822

Strand: Reverse

Name: bbvIM [H]

Synonym: CLB_2811

Alternate gene names: 153932387

Gene position: 2963822-2962770 (Counterclockwise)

Preceding gene: 153933621

Following gene: 153931350

Centisome position: 76.71

GC content: 33.24

Gene sequence:

>1053_bases
ATGATATTTCAATTAGGAGAATTGTTCTGTGGCCCGGGTGGGTTGGGCTATGGTGCAAAAACAGCAGAGATAAAAAATAA
TAAATATAAAATAGTTCATAAATGGGCGAATGACTATGATCGTGATACTTGCGATACTTATATACATAATATATGTCCAG
ATGATCCTGAATCAGTTATTTGTCAAGATGTAAGAAAATTAGATATAGATTCTCTTGAATCTATAGATGCGCTAGCATTT
GGGTTCCCATGTAACGATTTTTCAGTAGTAGGCGAACAAAAGGGGTTTAATGGAGAATATGGTCCTTTATATACTTATGG
GGTAAAAGTATTAAAAAAGCATAAGCCACAATGGTTTCTTGCTGAAAATGTGGGCGGTATAAGATCAGCAAATGATGGTG
GAGCATTAAAAAAAATTCTTAGAGATTTAGAAAAGTCAGGATATAGATTATATCCGCATTTGTATAAGTTTGAACAATAT
GGAATACCACAAGCACGTCATAGGGTAATTATTATAGGTATTAGAAAGGATTTACCTTTTGAATTTAAAGTTCCTTCTCC
AGAACCTTATAAGGATATGGATAATACATGTAGAACAGCTTTAAGAGTGCCACCTATACGAGAAAATGCACCAAATAATG
AATTGACAAAACAATCAGCAAAAGTAACGGAGAGATTAATGCACATAAAACCAGGGGAGAATGCTTTTACTGCGAACTTA
CCAGAACATTTACAGCTAAATGTAAAGGGAGCAAAGATAAGTCAAATATATAAAAGATTAGATCCTAATAAACCATCTTA
TACAGTAACAGGTTCAGGTGGTGGAGGTACACATATATATCATTTTGAAGAACCAAGAGCATTAACTAATAGAGAAAGGG
CTAGACTACAAACCTTTCCAGATACATATGAATTTAAAGGATCAAAAGAAAGTGTAAGACGTCAAATTGGAATGGCAGTT
CCAGTTAAAGGAGCAAAGATAATATTTGAAGCTGTATTAAAGACATTTGCAGAAATACCTTATGAAAGTGTAGAATGTAA
TGTTAATCTATAA

Upstream 100 bases:

>100_bases
GTAGAATTGGAAGAAATTGCAGAAGTAGTAGAAGTAAAATTTGAGAGAAATTTTATATTAACGAATGGAGAAAAAGTATA
AATAAGAGAGGTATTTATAA

Downstream 100 bases:

>100_bases
GGGGGAATAAGGGTATATGATTAATTATTGGTGGGTAACAAGACCTAAAAGAAGGTTAAATACAATTCCAGAATTTCTTT
CTACATTTGCAGAAATATCA

Product: DNA-cytosine methyltransferase family protein

Products: NA

Alternate protein names: M.BbvI; Cytosine-specific methyltransferase BbvI [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MIFQLGELFCGPGGLGYGAKTAEIKNNKYKIVHKWANDYDRDTCDTYIHNICPDDPESVICQDVRKLDIDSLESIDALAF
GFPCNDFSVVGEQKGFNGEYGPLYTYGVKVLKKHKPQWFLAENVGGIRSANDGGALKKILRDLEKSGYRLYPHLYKFEQY
GIPQARHRVIIIGIRKDLPFEFKVPSPEPYKDMDNTCRTALRVPPIRENAPNNELTKQSAKVTERLMHIKPGENAFTANL
PEHLQLNVKGAKISQIYKRLDPNKPSYTVTGSGGGGTHIYHFEEPRALTNRERARLQTFPDTYEFKGSKESVRRQIGMAV
PVKGAKIIFEAVLKTFAEIPYESVECNVNL

Sequences:

>Translated_350_residues
MIFQLGELFCGPGGLGYGAKTAEIKNNKYKIVHKWANDYDRDTCDTYIHNICPDDPESVICQDVRKLDIDSLESIDALAF
GFPCNDFSVVGEQKGFNGEYGPLYTYGVKVLKKHKPQWFLAENVGGIRSANDGGALKKILRDLEKSGYRLYPHLYKFEQY
GIPQARHRVIIIGIRKDLPFEFKVPSPEPYKDMDNTCRTALRVPPIRENAPNNELTKQSAKVTERLMHIKPGENAFTANL
PEHLQLNVKGAKISQIYKRLDPNKPSYTVTGSGGGGTHIYHFEEPRALTNRERARLQTFPDTYEFKGSKESVRRQIGMAV
PVKGAKIIFEAVLKTFAEIPYESVECNVNL
>Mature_350_residues
MIFQLGELFCGPGGLGYGAKTAEIKNNKYKIVHKWANDYDRDTCDTYIHNICPDDPESVICQDVRKLDIDSLESIDALAF
GFPCNDFSVVGEQKGFNGEYGPLYTYGVKVLKKHKPQWFLAENVGGIRSANDGGALKKILRDLEKSGYRLYPHLYKFEQY
GIPQARHRVIIIGIRKDLPFEFKVPSPEPYKDMDNTCRTALRVPPIRENAPNNELTKQSAKVTERLMHIKPGENAFTANL
PEHLQLNVKGAKISQIYKRLDPNKPSYTVTGSGGGGTHIYHFEEPRALTNRERARLQTFPDTYEFKGSKESVRRQIGMAV
PVKGAKIIFEAVLKTFAEIPYESVECNVNL

Specific function: This methylase recognizes the double-stranded sequence GCWGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the BbvI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=207, Percent_Identity=26.0869565217391, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 39549; Mature: 39549

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFQLGELFCGPGGLGYGAKTAEIKNNKYKIVHKWANDYDRDTCDTYIHNICPDDPESVI
CEEECCCHHCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHCCCCCHHHHH
CQDVRKLDIDSLESIDALAFGFPCNDFSVVGEQKGFNGEYGPLYTYGVKVLKKHKPQWFL
HHHHHHCCHHHHHHHHHHEECCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
AENVGGIRSANDGGALKKILRDLEKSGYRLYPHLYKFEQYGIPQARHRVIIIGIRKDLPF
ECCCCCCCCCCCCHHHHHHHHHHHHCCCEECHHHHHHHHCCCCHHHCEEEEEEECCCCCE
EFKVPSPEPYKDMDNTCRTALRVPPIRENAPNNELTKQSAKVTERLMHIKPGENAFTANL
EEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCC
PEHLQLNVKGAKISQIYKRLDPNKPSYTVTGSGGGGTHIYHFEEPRALTNRERARLQTFP
CCCEEEEECCHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHCCC
DTYEFKGSKESVRRQIGMAVPVKGAKIIFEAVLKTFAEIPYESVECNVNL
CCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECC
>Mature Secondary Structure
MIFQLGELFCGPGGLGYGAKTAEIKNNKYKIVHKWANDYDRDTCDTYIHNICPDDPESVI
CEEECCCHHCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHCCCCCHHHHH
CQDVRKLDIDSLESIDALAFGFPCNDFSVVGEQKGFNGEYGPLYTYGVKVLKKHKPQWFL
HHHHHHCCHHHHHHHHHHEECCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
AENVGGIRSANDGGALKKILRDLEKSGYRLYPHLYKFEQYGIPQARHRVIIIGIRKDLPF
ECCCCCCCCCCCCHHHHHHHHHHHHCCCEECHHHHHHHHCCCCHHHCEEEEEEECCCCCE
EFKVPSPEPYKDMDNTCRTALRVPPIRENAPNNELTKQSAKVTERLMHIKPGENAFTANL
EEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCC
PEHLQLNVKGAKISQIYKRLDPNKPSYTVTGSGGGGTHIYHFEEPRALTNRERARLQTFP
CCCEEEEECCHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHCCC
DTYEFKGSKESVRRQIGMAVPVKGAKIIFEAVLKTFAEIPYESVECNVNL
CCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA