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The map label for this gene is dat [H]

Identifier: 153931701

GI number: 153931701

Start: 2821004

End: 2821837

Strand: Reverse

Name: dat [H]

Synonym: CLB_2697

Alternate gene names: 153931701

Gene position: 2821837-2821004 (Counterclockwise)

Preceding gene: 153932968

Following gene: 153933311

Centisome position: 73.04

GC content: 23.86

Gene sequence:

>834_bases
ATGGAATGTTTTAATAAATTTTTTATAGAGAATGAGAAAATAAAAGAAATAAATCTATTTGATGAAAATTTCCTTAAAGA
AGGAAAATCTCTTTATGAAGTTATAAGGATAATAGATGGAGCGCCTTTGTTTTTAAAAAGCCATTTAAATAGATTTTACA
ACTCTGCAAAACTAGAAGAACTAAATTTATGGTTAGATGAAGAGGTAATAAAAGAAAAAATAAATAGACTTATAAAAATA
AATAAAGTATCTATAGGCAATATAAAGTTAGTATTTAATTTTAATAAAGAGAAGAATAACAAATTTTTATGCTATTTTTT
AAAACATAATTATCCAGAGGATATAGAGTATAAAAATGGAGTAAGAACTATACTTTATCATGGAGAAAGAGAAAATCCAA
ATGCAAAGGTTATAAATATGGATTTTAGAAAAGCTGTAGGAGAAAAGATAAAAGAAGAGAAAGCTTATGAAGCAATACTA
GTAGACAAAAATGGATACATAACCGAGGGAAGTAAATCTAATATATTTATGATAAAAGATAGTAAGGTAATAACATCGCC
TGTAGAAAAAGTATTACCAGGAATAACAAGACAAAATATAATAGATGTATGTAAAAATCTAAATTTGGATATTGATGAAG
AAAAAGTACATTATAAAGATATAGAAAAATTAGAGGGACTATTTATATCAGGTACATCACCAAAAGTTTTACCAATAAAA
TCTGTAGATGAAATGGAATTCAAATCTTCAGAGAATAAATTAATACTAAGTATTATGGAAGGTTATAATAAAGCAATAGA
GAAAGATATAAAAAGTTATAAAAGTAAAGAATAA

Upstream 100 bases:

>100_bases
ACTGATTGTGAATTTTAATTCAAACTAATTTAAGTGCTTTATAATATTAATACACAATATGAGGATTGTGATATTTAGAA
TAAATGGGGGATATATATTA

Downstream 100 bases:

>100_bases
TCATTTTAAAAATCAAATGGATATCACAGTGTAATTTTATAAGGCAGATAGCACAGCAAATTTATTGATAGATTGACAAA
ATATTGGATAAAAAATATAA

Product: putative branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MECFNKFFIENEKIKEINLFDENFLKEGKSLYEVIRIIDGAPLFLKSHLNRFYNSAKLEELNLWLDEEVIKEKINRLIKI
NKVSIGNIKLVFNFNKEKNNKFLCYFLKHNYPEDIEYKNGVRTILYHGERENPNAKVINMDFRKAVGEKIKEEKAYEAIL
VDKNGYITEGSKSNIFMIKDSKVITSPVEKVLPGITRQNIIDVCKNLNLDIDEEKVHYKDIEKLEGLFISGTSPKVLPIK
SVDEMEFKSSENKLILSIMEGYNKAIEKDIKSYKSKE

Sequences:

>Translated_277_residues
MECFNKFFIENEKIKEINLFDENFLKEGKSLYEVIRIIDGAPLFLKSHLNRFYNSAKLEELNLWLDEEVIKEKINRLIKI
NKVSIGNIKLVFNFNKEKNNKFLCYFLKHNYPEDIEYKNGVRTILYHGERENPNAKVINMDFRKAVGEKIKEEKAYEAIL
VDKNGYITEGSKSNIFMIKDSKVITSPVEKVLPGITRQNIIDVCKNLNLDIDEEKVHYKDIEKLEGLFISGTSPKVLPIK
SVDEMEFKSSENKLILSIMEGYNKAIEKDIKSYKSKE
>Mature_277_residues
MECFNKFFIENEKIKEINLFDENFLKEGKSLYEVIRIIDGAPLFLKSHLNRFYNSAKLEELNLWLDEEVIKEKINRLIKI
NKVSIGNIKLVFNFNKEKNNKFLCYFLKHNYPEDIEYKNGVRTILYHGERENPNAKVINMDFRKAVGEKIKEEKAYEAIL
VDKNGYITEGSKSNIFMIKDSKVITSPVEKVLPGITRQNIIDVCKNLNLDIDEEKVHYKDIEKLEGLFISGTSPKVLPIK
SVDEMEFKSSENKLILSIMEGYNKAIEKDIKSYKSKE

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=243, Percent_Identity=25.1028806584362, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 32378; Mature: 32378

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MECFNKFFIENEKIKEINLFDENFLKEGKSLYEVIRIIDGAPLFLKSHLNRFYNSAKLEE
CCCHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHH
LNLWLDEEVIKEKINRLIKINKVSIGNIKLVFNFNKEKNNKFLCYFLKHNYPEDIEYKNG
HHCCCCHHHHHHHHHHHEEEEEEEECCEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCC
VRTILYHGERENPNAKVINMDFRKAVGEKIKEEKAYEAILVDKNGYITEGSKSNIFMIKD
CEEEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHHEEEEECCCCEEECCCCCCEEEEEC
SKVITSPVEKVLPGITRQNIIDVCKNLNLDIDEEKVHYKDIEKLEGLFISGTSPKVLPIK
CCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEECCCCCEEEEC
SVDEMEFKSSENKLILSIMEGYNKAIEKDIKSYKSKE
CCCHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MECFNKFFIENEKIKEINLFDENFLKEGKSLYEVIRIIDGAPLFLKSHLNRFYNSAKLEE
CCCHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHH
LNLWLDEEVIKEKINRLIKINKVSIGNIKLVFNFNKEKNNKFLCYFLKHNYPEDIEYKNG
HHCCCCHHHHHHHHHHHEEEEEEEECCEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCC
VRTILYHGERENPNAKVINMDFRKAVGEKIKEEKAYEAILVDKNGYITEGSKSNIFMIKD
CEEEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHHEEEEECCCCEEECCCCCCEEEEEC
SKVITSPVEKVLPGITRQNIIDVCKNLNLDIDEEKVHYKDIEKLEGLFISGTSPKVLPIK
CCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEECCCCCEEEEC
SVDEMEFKSSENKLILSIMEGYNKAIEKDIKSYKSKE
CCCHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9003455 [H]