Click here to switch to the map view.

The map label for this gene is lip2 [H]

Identifier: 153931081

GI number: 153931081

Start: 2262551

End: 2263582

Strand: Reverse

Name: lip2 [H]

Synonym: CLB_2125

Alternate gene names: 153931081

Gene position: 2263582-2262551 (Counterclockwise)

Preceding gene: 153931901

Following gene: 153934013

Centisome position: 58.59

GC content: 31.1

Gene sequence:

>1032_bases
GTGAAAAAAGTAATTAAAGTAGTATCAGTTATTCTAGTTATACTTGTAATAGCAGGATTTTTTATTATTAAGAATTTAAC
TGAGACCAAGGATGGTAAGCTTAATATGTATGTTGCTGCAAACTTACAGTTATATAAAATTCTTAATCCTAAATCACTCA
ATAATAAATCAATTGAAGAAACTAGAGGAGCTTTAAATAAGCAATCAACTAGGTGGTCAAATAAACCTATACCATTTTCT
AATATTAAGAACCTTGATATAAAAATGAACAATGAGAAAATACCAGTAAGAATATATACACCTGAAAAGGGTAGCAATTT
CCCTATAATCATTTATTCACATGGTGGTTTCTGGATTGGAGGAAATGTTGATACTATTGATGGGGTTTGTAGAAAGCTTT
CACAGAATACAAAAGCAATTGTAATATCTGTAAACTATAGGCTTGCCCCAGAAAATCCTTTCCCCGCTGGTCTTAATGAT
GTATATAATGTGCTTCAGTGGACTTATAAAAATGGAAAAAGCATAAATGGAGATGAAAAACATATAGCAGTTGTAGGGGA
CAGTGCAGGTGGAAATCTTTCTGCTGCAGTTTCATCAATGTCACGGGATAAAAATGGACCTTCTATAACATGTCAGGTAT
TAATATATCCATCAACAAATATATTTAAACTGAACAGTAAATCTTGGTCTCATTTTTCTAATAGCTTTAATGTTTCAACA
GAGGATATGGAAAAGTATATTTCAATTTATGCACCAAAAAAAGAAGATAGAAAAAGCCCTTATGCATCCCCACTTTTATC
TAAGGATCTTAGAAAATTACCTGATACACTCGTAGTGACAGCAGAAATTGATCCTCTTAGAGATGAAGGGGAAGCTTATG
CTAATAAACTAAAAGAATCTGGGGTTAAAGCAGAGGTTACTAGATATAAAGGTATTACTCATGGATTTATTACAATGGAT
AAGATTACAAATAAAGCAGACGAAGCATTAAATCAAATTTCTCTATATATACAAAAAGAGTTTCAAAAATAA

Upstream 100 bases:

>100_bases
CAAAGATTTTAAGTATATTAAAATGCGTAAAGGAAAAAATATATAAGTAAATTTAGTTATTTTTATTGCAGAATTTCTAT
AGTGGATGGAGGTAAATGGA

Downstream 100 bases:

>100_bases
ACTTAATATAATTATTTTGCTACAAAATTTATTATAGAGGATGTTGCAGAGATAAAAGTTAAGTTTAGTTGACAAATTGC
CATCTTTACTTGATAGAAAA

Product: putative lipase/esterase

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKKVIKVVSVILVILVIAGFFIIKNLTETKDGKLNMYVAANLQLYKILNPKSLNNKSIEETRGALNKQSTRWSNKPIPFS
NIKNLDIKMNNEKIPVRIYTPEKGSNFPIIIYSHGGFWIGGNVDTIDGVCRKLSQNTKAIVISVNYRLAPENPFPAGLND
VYNVLQWTYKNGKSINGDEKHIAVVGDSAGGNLSAAVSSMSRDKNGPSITCQVLIYPSTNIFKLNSKSWSHFSNSFNVST
EDMEKYISIYAPKKEDRKSPYASPLLSKDLRKLPDTLVVTAEIDPLRDEGEAYANKLKESGVKAEVTRYKGITHGFITMD
KITNKADEALNQISLYIQKEFQK

Sequences:

>Translated_343_residues
MKKVIKVVSVILVILVIAGFFIIKNLTETKDGKLNMYVAANLQLYKILNPKSLNNKSIEETRGALNKQSTRWSNKPIPFS
NIKNLDIKMNNEKIPVRIYTPEKGSNFPIIIYSHGGFWIGGNVDTIDGVCRKLSQNTKAIVISVNYRLAPENPFPAGLND
VYNVLQWTYKNGKSINGDEKHIAVVGDSAGGNLSAAVSSMSRDKNGPSITCQVLIYPSTNIFKLNSKSWSHFSNSFNVST
EDMEKYISIYAPKKEDRKSPYASPLLSKDLRKLPDTLVVTAEIDPLRDEGEAYANKLKESGVKAEVTRYKGITHGFITMD
KITNKADEALNQISLYIQKEFQK
>Mature_343_residues
MKKVIKVVSVILVILVIAGFFIIKNLTETKDGKLNMYVAANLQLYKILNPKSLNNKSIEETRGALNKQSTRWSNKPIPFS
NIKNLDIKMNNEKIPVRIYTPEKGSNFPIIIYSHGGFWIGGNVDTIDGVCRKLSQNTKAIVISVNYRLAPENPFPAGLND
VYNVLQWTYKNGKSINGDEKHIAVVGDSAGGNLSAAVSSMSRDKNGPSITCQVLIYPSTNIFKLNSKSWSHFSNSFNVST
EDMEKYISIYAPKKEDRKSPYASPLLSKDLRKLPDTLVVTAEIDPLRDEGEAYANKLKESGVKAEVTRYKGITHGFITMD
KITNKADEALNQISLYIQKEFQK

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=304, Percent_Identity=28.2894736842105, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI61966717, Length=289, Percent_Identity=26.2975778546713, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI157041239, Length=288, Percent_Identity=25, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI206597554, Length=290, Percent_Identity=27.5862068965517, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI68051721, Length=167, Percent_Identity=26.9461077844311, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786682, Length=282, Percent_Identity=26.5957446808511, Blast_Score=108, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17567059, Length=276, Percent_Identity=31.5217391304348, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17540028, Length=266, Percent_Identity=28.1954887218045, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71996133, Length=269, Percent_Identity=27.1375464684015, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI72001146, Length=143, Percent_Identity=32.8671328671329, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 38330; Mature: 38330

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVIKVVSVILVILVIAGFFIIKNLTETKDGKLNMYVAANLQLYKILNPKSLNNKSIEE
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECEEEEEEECCCCCCCCCHHH
TRGALNKQSTRWSNKPIPFSNIKNLDIKMNNEKIPVRIYTPEKGSNFPIIIYSHGGFWIG
HHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCEEEECCCCEEEC
GNVDTIDGVCRKLSQNTKAIVISVNYRLAPENPFPAGLNDVYNVLQWTYKNGKSINGDEK
CCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
HIAVVGDSAGGNLSAAVSSMSRDKNGPSITCQVLIYPSTNIFKLNSKSWSHFSNSFNVST
EEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEECCCCCHHCCCCCCCCH
EDMEKYISIYAPKKEDRKSPYASPLLSKDLRKLPDTLVVTAEIDPLRDEGEAYANKLKES
HHHHHHHEEECCCCHHCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHC
GVKAEVTRYKGITHGFITMDKITNKADEALNQISLYIQKEFQK
CCEEEEHHHCCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKVIKVVSVILVILVIAGFFIIKNLTETKDGKLNMYVAANLQLYKILNPKSLNNKSIEE
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECEEEEEEECCCCCCCCCHHH
TRGALNKQSTRWSNKPIPFSNIKNLDIKMNNEKIPVRIYTPEKGSNFPIIIYSHGGFWIG
HHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCEEEECCCCEEEC
GNVDTIDGVCRKLSQNTKAIVISVNYRLAPENPFPAGLNDVYNVLQWTYKNGKSINGDEK
CCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
HIAVVGDSAGGNLSAAVSSMSRDKNGPSITCQVLIYPSTNIFKLNSKSWSHFSNSFNVST
EEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEECCCCCHHCCCCCCCCH
EDMEKYISIYAPKKEDRKSPYASPLLSKDLRKLPDTLVVTAEIDPLRDEGEAYANKLKES
HHHHHHHEEECCCCHHCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHC
GVKAEVTRYKGITHGFITMDKITNKADEALNQISLYIQKEFQK
CCEEEEHHHCCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]