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The map label for this gene is rnjA [H]

Identifier: 153002975

GI number: 153002975

Start: 115610

End: 117262

Strand: Reverse

Name: rnjA [H]

Synonym: Anae109_0096

Alternate gene names: 153002975

Gene position: 117262-115610 (Counterclockwise)

Preceding gene: 153002978

Following gene: 153002974

Centisome position: 2.22

GC content: 74.83

Gene sequence:

>1653_bases
GTGGCGCCCCCCGTCCGCATCGTGCCGCTCGGCGGCCTCGGCGAGATCGGCATGAACTGCATGGCGATCGAGGCCGACGG
CCGGATGGCCGTGGTGGACTGCGGGATCCTCTTCCCGAACGAGCCCATCGGCGTGGACGTGATCGCGCCGGACCTCGGCT
GGCTGCGCGAGCGGCGCGAGCAGGTCGGCGCCATCTACCTGACCCACGGGCACGAGGATCACATCGGCGCGCTGCCGTTC
CTGCTGCGCGAGGTGAAGGCGCCGGTCTACGGAACGCGCTTCACGCTCGCGCTCCTGCGCCCGCGGCTCGAGGAGGCGGG
CGTCGAGGCGGACCTGCGCGAGGTGGCGCCCGGCGACGCGCGACCGGCGGGCGAGGCGTCTCCCCTCTCGGCCGAGTTCT
ACGCCGTCACCCACTCCATCCCCGACGCGTGCGGGCTCGCGCTGTCGACGCCCCAGGGCACGATCCTCCACTCCGGGGAC
TTCAAGATCGACCCGCGCCCGATCTCCGGGCCCGGCTTCGATCTCGCCCGCGTCGAGGCGCTCGGCCGCCGCGGCGTGCG
CCTCCTGCTCTCCGACTCGACGAACTCCGAGCGGCCGGGCTCCTCCCTCTCCGAGGCCGAGGTCGGGGCAGCCCTCGAGG
AGGCGTTCGAGCGCGCCCCCGCACGCGTCTTCGTCGCCTGCTTCGCCTCCAACATCCACCGCATCCAGCAGGTGGCGAAC
GCCGCGCGCGCGTTCGGCCGGCGGCTCGCGCTCCTCGGCCGCTCGATGGAGACGAACGTCCGGCTCGCCCAGGCGCTCGG
GTACCTGGAGCTGCCCGGCTGGATGCCGGTGGGGTTCCCGGAGGCGCGCGAGCTGCCGCCGAAGGAGCTCTGCGTCCTCA
CCACCGGCACCCAGGGCGAGCCGCGGAGCGCGCTCGCGCGGCTCGCGCGCGGCGAGCACCTGGACCTCGACGTCGCGCCG
GGTGATCTCGTCGTCCTCTCCTCGCGCTTCATCCCAGGCAACGAGGTGGCGGTCGGCGAGCTGGTGAACGCGCTCTGCCG
GCGCGGGGCGCAGGTGGCCTACGAGGGGCTCCGGCCGCTGCACGTGTCCGGCCACGCCCAGGAGGCGGAGCAGCGGCGGC
TCATCGCGCTCGCCCGGCCCGAGCACTTCGTCCCCATTCACGGAGAGTACCGTCACCTCGCGCGCCACGCGGCCCACGCC
GCCGCGGAGGGGGTGCGCGGGCGGCACCTGCTCCTCGACGGGCAGGTCCTCGAGCTCGGCGACCTCGGCGCGACCGTGCT
CGAGGAGCCGGTCCCGGTCGGGCGCGTCTACACCGACCGGGACGCGCTGCTCGGGCAGGACATCGGCGCGCTCGTGGTGA
AGGACCGGCGCCTGCTCGCCGAGGCCGGGCTCTGCATCGCGGTGCTCGCCGTGGACAAGGTGACCGGCGCGGTGATACGC
GGGCCGGAGCTCTTCGCCCGGGGCGTCGCCGGGTTCGAGGGGGCGGAGGAGGAGATCCGGGCGGAGGCGCTGCGCGCCCT
CGACGAGCTCACGCCGCAGGCCCGCGTGGCGATCGAGGAGGTGCAGGAGACGCTCCGGGTCGCGGTCCGCCGCTTCCTGA
AGCGCACCACGGGGAAGCGGCCCACGGTGCTGCCGGTGGTGCTGGAACTCTAG

Upstream 100 bases:

>100_bases
CGGCCCGCCGCGAAGGCGACGGGCGGAGCGGGTGGGGGGCCCGGTGCCAGGGAACCGGGTTCCCGCCCCTCCACGCGCCC
CCAGGGAGTAGATTCCCCGC

Downstream 100 bases:

>100_bases
AGCTCTGGGCTCAAAGCTTCGCGCAACGGGCTTCGGGAGAGGCGCCCGCCATCGCGAACGGCCCGCACGAGGGAGAGCGG
TATGCCGAGCTTCGACGTGG

Product: beta-lactamase domain-containing protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MAPPVRIVPLGGLGEIGMNCMAIEADGRMAVVDCGILFPNEPIGVDVIAPDLGWLRERREQVGAIYLTHGHEDHIGALPF
LLREVKAPVYGTRFTLALLRPRLEEAGVEADLREVAPGDARPAGEASPLSAEFYAVTHSIPDACGLALSTPQGTILHSGD
FKIDPRPISGPGFDLARVEALGRRGVRLLLSDSTNSERPGSSLSEAEVGAALEEAFERAPARVFVACFASNIHRIQQVAN
AARAFGRRLALLGRSMETNVRLAQALGYLELPGWMPVGFPEARELPPKELCVLTTGTQGEPRSALARLARGEHLDLDVAP
GDLVVLSSRFIPGNEVAVGELVNALCRRGAQVAYEGLRPLHVSGHAQEAEQRRLIALARPEHFVPIHGEYRHLARHAAHA
AAEGVRGRHLLLDGQVLELGDLGATVLEEPVPVGRVYTDRDALLGQDIGALVVKDRRLLAEAGLCIAVLAVDKVTGAVIR
GPELFARGVAGFEGAEEEIRAEALRALDELTPQARVAIEEVQETLRVAVRRFLKRTTGKRPTVLPVVLEL

Sequences:

>Translated_550_residues
MAPPVRIVPLGGLGEIGMNCMAIEADGRMAVVDCGILFPNEPIGVDVIAPDLGWLRERREQVGAIYLTHGHEDHIGALPF
LLREVKAPVYGTRFTLALLRPRLEEAGVEADLREVAPGDARPAGEASPLSAEFYAVTHSIPDACGLALSTPQGTILHSGD
FKIDPRPISGPGFDLARVEALGRRGVRLLLSDSTNSERPGSSLSEAEVGAALEEAFERAPARVFVACFASNIHRIQQVAN
AARAFGRRLALLGRSMETNVRLAQALGYLELPGWMPVGFPEARELPPKELCVLTTGTQGEPRSALARLARGEHLDLDVAP
GDLVVLSSRFIPGNEVAVGELVNALCRRGAQVAYEGLRPLHVSGHAQEAEQRRLIALARPEHFVPIHGEYRHLARHAAHA
AAEGVRGRHLLLDGQVLELGDLGATVLEEPVPVGRVYTDRDALLGQDIGALVVKDRRLLAEAGLCIAVLAVDKVTGAVIR
GPELFARGVAGFEGAEEEIRAEALRALDELTPQARVAIEEVQETLRVAVRRFLKRTTGKRPTVLPVVLEL
>Mature_549_residues
APPVRIVPLGGLGEIGMNCMAIEADGRMAVVDCGILFPNEPIGVDVIAPDLGWLRERREQVGAIYLTHGHEDHIGALPFL
LREVKAPVYGTRFTLALLRPRLEEAGVEADLREVAPGDARPAGEASPLSAEFYAVTHSIPDACGLALSTPQGTILHSGDF
KIDPRPISGPGFDLARVEALGRRGVRLLLSDSTNSERPGSSLSEAEVGAALEEAFERAPARVFVACFASNIHRIQQVANA
ARAFGRRLALLGRSMETNVRLAQALGYLELPGWMPVGFPEARELPPKELCVLTTGTQGEPRSALARLARGEHLDLDVAPG
DLVVLSSRFIPGNEVAVGELVNALCRRGAQVAYEGLRPLHVSGHAQEAEQRRLIALARPEHFVPIHGEYRHLARHAAHAA
AEGVRGRHLLLDGQVLELGDLGATVLEEPVPVGRVYTDRDALLGQDIGALVVKDRRLLAEAGLCIAVLAVDKVTGAVIRG
PELFARGVAGFEGAEEEIRAEALRALDELTPQARVAIEEVQETLRVAVRRFLKRTTGKRPTVLPVVLEL

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 59131; Mature: 59000

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPPVRIVPLGGLGEIGMNCMAIEADGRMAVVDCGILFPNEPIGVDVIAPDLGWLRERRE
CCCCEEEEECCCCHHHCCEEEEEECCCCEEEEEEEEECCCCCCCEEEECCCHHHHHHHHH
QVGAIYLTHGHEDHIGALPFLLREVKAPVYGTRFTLALLRPRLEEAGVEADLREVAPGDA
HCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCC
RPAGEASPLSAEFYAVTHSIPDACGLALSTPQGTILHSGDFKIDPRPISGPGFDLARVEA
CCCCCCCCCCEEEEEEECCCCHHHCCEEECCCCCEEECCCCEECCCCCCCCCCCHHHHHH
LGRRGVRLLLSDSTNSERPGSSLSEAEVGAALEEAFERAPARVFVACFASNIHRIQQVAN
HHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AARAFGRRLALLGRSMETNVRLAQALGYLELPGWMPVGFPEARELPPKELCVLTTGTQGE
HHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCCCCCCCCCCHHCCCHHHEEEEECCCCCC
PRSALARLARGEHLDLDVAPGDLVVLSSRFIPGNEVAVGELVNALCRRGAQVAYEGLRPL
HHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCEE
HVSGHAQEAEQRRLIALARPEHFVPIHGEYRHLARHAAHAAAEGVRGRHLLLDGQVLELG
EECCCHHHHHHCCEEEEECCCCEEECCCHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEC
DLGATVLEEPVPVGRVYTDRDALLGQDIGALVVKDRRLLAEAGLCIAVLAVDKVTGAVIR
CCCHHHHHCCCCCCCEECCCHHHHCCCCCCEEHHHHHHHHHCCCEEEEEHHHHHHHHHEE
GPELFARGVAGFEGAEEEIRAEALRALDELTPQARVAIEEVQETLRVAVRRFLKRTTGKR
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PTVLPVVLEL
CEEEEEEEEC
>Mature Secondary Structure 
APPVRIVPLGGLGEIGMNCMAIEADGRMAVVDCGILFPNEPIGVDVIAPDLGWLRERRE
CCCEEEEECCCCHHHCCEEEEEECCCCEEEEEEEEECCCCCCCEEEECCCHHHHHHHHH
QVGAIYLTHGHEDHIGALPFLLREVKAPVYGTRFTLALLRPRLEEAGVEADLREVAPGDA
HCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCC
RPAGEASPLSAEFYAVTHSIPDACGLALSTPQGTILHSGDFKIDPRPISGPGFDLARVEA
CCCCCCCCCCEEEEEEECCCCHHHCCEEECCCCCEEECCCCEECCCCCCCCCCCHHHHHH
LGRRGVRLLLSDSTNSERPGSSLSEAEVGAALEEAFERAPARVFVACFASNIHRIQQVAN
HHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AARAFGRRLALLGRSMETNVRLAQALGYLELPGWMPVGFPEARELPPKELCVLTTGTQGE
HHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCCCCCCCCCCHHCCCHHHEEEEECCCCCC
PRSALARLARGEHLDLDVAPGDLVVLSSRFIPGNEVAVGELVNALCRRGAQVAYEGLRPL
HHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCEE
HVSGHAQEAEQRRLIALARPEHFVPIHGEYRHLARHAAHAAAEGVRGRHLLLDGQVLELG
EECCCHHHHHHCCEEEEECCCCEEECCCHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEC
DLGATVLEEPVPVGRVYTDRDALLGQDIGALVVKDRRLLAEAGLCIAVLAVDKVTGAVIR
CCCHHHHHCCCCCCCEECCCHHHHCCCCCCEEHHHHHHHHHCCCEEEEEHHHHHHHHHEE
GPELFARGVAGFEGAEEEIRAEALRALDELTPQARVAIEEVQETLRVAVRRFLKRTTGKR
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PTVLPVVLEL
CEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]