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The map label for this gene is rimO

Identifier: 153002973

GI number: 153002973

Start: 113569

End: 114981

Strand: Reverse

Name: rimO

Synonym: Anae109_0094

Alternate gene names: 153002973

Gene position: 114981-113569 (Counterclockwise)

Preceding gene: 153002974

Following gene: 153002972

Centisome position: 2.18

GC content: 72.47

Gene sequence:

>1413_bases
ATGGCGACCACCCGCGTCTACCTCCACACGCTCGGCTGCCCGAAGAACCGGGTGGACAGCGAGGTCATGCTCGGCACCCT
GACGGGCGCCGGCTACCGGCTCGAGCGCGACCCCGCCCAGGCGGACGTGATCGTGGTGAACACCTGCGGCTTCATCGAGA
GCGCCAAGGAGGAGTCGGTCGACGCCATCGTCGAGCTCGCGGGGATGAAGCAGGAGGGGCGCTGCAAGAAGCTCGTCGTG
ACCGGCTGCCTGGTGCAGCGCCACGCCGAGGAGCTGTCGGCGGAGCTGCCCGAGGTGGACCACTTCCTCGGCACCGGCGC
GTACGCGGAGATCGCGCGGGTGGTCTCCGACGCGCAGGCGAAGCGGCTCGTCGTGCCCGACCCCGACTTCGTGCACTCCG
CGGCCACGCCGCGCGTGAACTCGCTCCCCTCGCACACCGCCTACCTCAAGATCTCCGAGGGCTGCGACAACGCCTGCGCC
TTCTGCATCATCCCCAAGCTGCGCGGCGCGCAGCGCTCGCGGCCGGTGGACGACGTGGTCGCGGAGGCCGCCGCCCTCGC
GGCGCAAGGCACGGTGGAGCTGTCGCTCGTGGCGCAGGATCTCACCGCCTACGGCTACGACCTGCCCGGCAAGGTGCGGC
TGCATCACCTCTTGCCCGAGCTCTGCAAGGTGGACGGCATCCGGTGGCTCCGGCTGCACTACGCCTACCCGCGCGACGTG
CCGGACGCGCTCGTGGAGGTGATCGCCCGCGAGCCGAAGATCGTGAAGTACCTCGACATGCCGCTCCAGCACTCCTCGGA
CCGGCTGCTCCGCTCGATGAAGCGCGGCCGCGACTCGGTGTTCCTGCGCGAGCTGCTCGCGCGGCTGCGCGCGCGGGTGC
CGGGGATCGCGCTCCGCACCTCGCTCATCGTCGGGCTCCCCGGCGAGACCGAGGAGGACTTCGAGGATCTCGTGCGCTTC
GTGGAGGAGCAGCGCTTCGAGCGGCTCGGCGTGTTCGAGTTCTCGCCCGAGGACGGCACGCCCGCGGCGGACATGGCGGA
GCAGGTGCCGGACGTGGTGAAGCGCGCGCGGCGCGACCGGATCATGGCGCTCCAGCAGGAGATCTCGCGCGAGCACCAGC
GGGCGATGGTCGGCCGCCGGCTGGAGGTGCTCGTGGAGGGCCGCGCCGAGGAGACGGAGCACCTCCTCGCCGGGCGCCAC
GCCCAGCAGGCCCCGGAGATCGACGGGATCACCTACGTGAACGACGGCGTCGCCTACCCCGGCGAGCTCGTCACCGTCGA
GATCACCGACGCCTCCGAGTACGACCTCGTCGGCCACGTGGTGGCGCGCGATCCGGAGCGGGCGCGGCGACCGCTGCCCG
CGCCCGCCGGAGGCGAGACGCCGCGCCGCGGCGGGCTGCCGGTCGTGGGCTGA

Upstream 100 bases:

>100_bases
GGCCTGGGGGTGCCGCTCCAGTTCCAGAACTTCCGGGAGTGAGGGGACCGGGAGCGCGGATTGTCCCGCCCCTCCCCGTC
TGTTAGATCCGCCGGCTCCC

Downstream 100 bases:

>100_bases
TCGAGCCGGCCCCGCCGCGGAGGCGCGGGCCGGCACCGCCTCGCCGCACGCGACGCCGCGCAGTCAATGCGCTGGATCCC
TGCTGGGTTTCGCGCGACAG

Product: MiaB-like tRNA modifying protein YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MATTRVYLHTLGCPKNRVDSEVMLGTLTGAGYRLERDPAQADVIVVNTCGFIESAKEESVDAIVELAGMKQEGRCKKLVV
TGCLVQRHAEELSAELPEVDHFLGTGAYAEIARVVSDAQAKRLVVPDPDFVHSAATPRVNSLPSHTAYLKISEGCDNACA
FCIIPKLRGAQRSRPVDDVVAEAAALAAQGTVELSLVAQDLTAYGYDLPGKVRLHHLLPELCKVDGIRWLRLHYAYPRDV
PDALVEVIAREPKIVKYLDMPLQHSSDRLLRSMKRGRDSVFLRELLARLRARVPGIALRTSLIVGLPGETEEDFEDLVRF
VEEQRFERLGVFEFSPEDGTPAADMAEQVPDVVKRARRDRIMALQQEISREHQRAMVGRRLEVLVEGRAEETEHLLAGRH
AQQAPEIDGITYVNDGVAYPGELVTVEITDASEYDLVGHVVARDPERARRPLPAPAGGETPRRGGLPVVG

Sequences:

>Translated_470_residues
MATTRVYLHTLGCPKNRVDSEVMLGTLTGAGYRLERDPAQADVIVVNTCGFIESAKEESVDAIVELAGMKQEGRCKKLVV
TGCLVQRHAEELSAELPEVDHFLGTGAYAEIARVVSDAQAKRLVVPDPDFVHSAATPRVNSLPSHTAYLKISEGCDNACA
FCIIPKLRGAQRSRPVDDVVAEAAALAAQGTVELSLVAQDLTAYGYDLPGKVRLHHLLPELCKVDGIRWLRLHYAYPRDV
PDALVEVIAREPKIVKYLDMPLQHSSDRLLRSMKRGRDSVFLRELLARLRARVPGIALRTSLIVGLPGETEEDFEDLVRF
VEEQRFERLGVFEFSPEDGTPAADMAEQVPDVVKRARRDRIMALQQEISREHQRAMVGRRLEVLVEGRAEETEHLLAGRH
AQQAPEIDGITYVNDGVAYPGELVTVEITDASEYDLVGHVVARDPERARRPLPAPAGGETPRRGGLPVVG
>Mature_469_residues
ATTRVYLHTLGCPKNRVDSEVMLGTLTGAGYRLERDPAQADVIVVNTCGFIESAKEESVDAIVELAGMKQEGRCKKLVVT
GCLVQRHAEELSAELPEVDHFLGTGAYAEIARVVSDAQAKRLVVPDPDFVHSAATPRVNSLPSHTAYLKISEGCDNACAF
CIIPKLRGAQRSRPVDDVVAEAAALAAQGTVELSLVAQDLTAYGYDLPGKVRLHHLLPELCKVDGIRWLRLHYAYPRDVP
DALVEVIAREPKIVKYLDMPLQHSSDRLLRSMKRGRDSVFLRELLARLRARVPGIALRTSLIVGLPGETEEDFEDLVRFV
EEQRFERLGVFEFSPEDGTPAADMAEQVPDVVKRARRDRIMALQQEISREHQRAMVGRRLEVLVEGRAEETEHLLAGRHA
QQAPEIDGITYVNDGVAYPGELVTVEITDASEYDLVGHVVARDPERARRPLPAPAGGETPRRGGLPVVG

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=481, Percent_Identity=29.1060291060291, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=29.1060291060291, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI93277076, Length=456, Percent_Identity=29.6052631578947, Blast_Score=175, Evalue=9e-44,
Organism=Escherichia coli, GI1787057, Length=443, Percent_Identity=39.9548532731377, Blast_Score=288, Evalue=7e-79,
Organism=Escherichia coli, GI1786882, Length=464, Percent_Identity=30.8189655172414, Blast_Score=219, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17553146, Length=469, Percent_Identity=26.0127931769723, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71996771, Length=319, Percent_Identity=32.2884012539185, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21356207, Length=429, Percent_Identity=28.4382284382284, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI19922432, Length=467, Percent_Identity=29.3361884368308, Blast_Score=156, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_ANADF (A7H6G8)

Other databases:

- EMBL:   CP000769
- RefSeq:   YP_001377298.1
- ProteinModelPortal:   A7H6G8
- SMR:   A7H6G8
- STRING:   A7H6G8
- GeneID:   5374426
- GenomeReviews:   CP000769_GR
- KEGG:   afw:Anae109_0094
- NMPDR:   fig|404589.4.peg.89
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK944817
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51798; Mature: 51667

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTRVYLHTLGCPKNRVDSEVMLGTLTGAGYRLERDPAQADVIVVNTCGFIESAKEESV
CCCEEEEEEECCCCCCCCCCHHEEEEECCCCEEECCCCCCCCEEEEECCCCHHHHHHHHH
DAIVELAGMKQEGRCKKLVVTGCLVQRHAEELSAELPEVDHFLGTGAYAEIARVVSDAQA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHH
KRLVVPDPDFVHSAATPRVNSLPSHTAYLKISEGCDNACAFCIIPKLRGAQRSRPVDDVV
CEEECCCCHHHHCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHH
AEAAALAAQGTVELSLVAQDLTAYGYDLPGKVRLHHLLPELCKVDGIRWLRLHYAYPRDV
HHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCH
PDALVEVIAREPKIVKYLDMPLQHSSDRLLRSMKRGRDSVFLRELLARLRARVPGIALRT
HHHHHHHHHCCCCEEHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEE
SLIVGLPGETEEDFEDLVRFVEEQRFERLGVFEFSPEDGTPAADMAEQVPDVVKRARRDR
EEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHH
IMALQQEISREHQRAMVGRRLEVLVEGRAEETEHLLAGRHAQQAPEIDGITYVNDGVAYP
HHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHCCCHHCCCCCCCEEEECCCCCCC
GELVTVEITDASEYDLVGHVVARDPERARRPLPAPAGGETPRRGGLPVVG
CCEEEEEECCCCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATTRVYLHTLGCPKNRVDSEVMLGTLTGAGYRLERDPAQADVIVVNTCGFIESAKEESV
CCEEEEEEECCCCCCCCCCHHEEEEECCCCEEECCCCCCCCEEEEECCCCHHHHHHHHH
DAIVELAGMKQEGRCKKLVVTGCLVQRHAEELSAELPEVDHFLGTGAYAEIARVVSDAQA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHH
KRLVVPDPDFVHSAATPRVNSLPSHTAYLKISEGCDNACAFCIIPKLRGAQRSRPVDDVV
CEEECCCCHHHHCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHH
AEAAALAAQGTVELSLVAQDLTAYGYDLPGKVRLHHLLPELCKVDGIRWLRLHYAYPRDV
HHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCH
PDALVEVIAREPKIVKYLDMPLQHSSDRLLRSMKRGRDSVFLRELLARLRARVPGIALRT
HHHHHHHHHCCCCEEHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEE
SLIVGLPGETEEDFEDLVRFVEEQRFERLGVFEFSPEDGTPAADMAEQVPDVVKRARRDR
EEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHH
IMALQQEISREHQRAMVGRRLEVLVEGRAEETEHLLAGRHAQQAPEIDGITYVNDGVAYP
HHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHCCCHHCCCCCCCEEEECCCCCCC
GELVTVEITDASEYDLVGHVVARDPERARRPLPAPAGGETPRRGGLPVVG
CCEEEEEECCCCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA