Click here to switch to the map view.

The map label for this gene is trmE [H]

Identifier: 152977683

GI number: 152977683

Start: 4083318

End: 4084694

Strand: Reverse

Name: trmE [H]

Synonym: Bcer98_4024

Alternate gene names: 152977683

Gene position: 4084694-4083318 (Counterclockwise)

Preceding gene: 152977684

Following gene: 152977682

Centisome position: 99.94

GC content: 36.09

Gene sequence:

>1377_bases
ATGGAATTTGATACAATTGCTGCGATTTCCACGGCACTTGGGGAGGGCGCGATTGCCATTGTTCGAGTAAGTGGAGAGGA
TGCGATTGAGAAGGTCAATCGCATTTTTAAAGGAAAAGATTTAACAGCAGTATCTTCTCATACAATTCATTATGGTCACA
TTGTGGATTTAGATACAAATCAAGTTATTGAGGAAGTTATGGTATCTATTATGCGTGCTCCTAAGACATTTACACGTGAA
GATATCGTAGAAGTGAACTGCCATGGTGGTCTTGTTTCTGTAAATAAAGTATTGCAACTGATTTTAGCACAAGGAGTACG
TTTAGCTGAGCCTGGTGAGTTTACGAAGCGTGCTTTTTTAAATGGTCGTATTGATTTATCTCAAGCTGAGGCAGTAATGG
ATTTAATCCGTGCAAAAACAGATCGTGCAATGAATGTAGCGATTAATCAAATGGAAGGACGTTTATCCAAATTGATTGGT
CGTCTTCGTCAAGAAATTTTAGAAACGTTAGCTCATGTTGAGGTAAATATAGATTACCCGGAATATGATGACGTGGAGGA
AATGACACATCGTATTTTAATTGAAAAAGCAACTCATGTTCAAAATGAGATTGAAAAGATTTTGGAAACATCAAAGCAAG
GAAAAATTTTGCGAGAAGGAATTGCAACAGCAATTATCGGAAGACCAAATGTCGGAAAATCTTCATTATTAAATAGTCTT
GTTCAGGAGAAAAAGGCTATTGTAACAGATATAGCAGGAACAACTCGTGATGTTATTGAAGAATATGTCAACGTACGTGG
TGTACCACTTCGACTTATTGATACAGCTGGAATTCGCGAAACAGAAGATATTGTCGAACAAATTGGTGTTGAGCGATCAA
AAGAGATGATGAGCCAAGCTGATTTAGTATTAATAGTAGTAAATTATAGTGAGCCATTAACGAATGAAGATGAAGAGCTA
TTCCGTGCTGTACAAGGAAAAGATTTCATTGTCATTGTAAATAAAACAGATTTACCACAAAAGATTGAGATGGAACGCGT
AACAGAATTAGCGAGTGAGAAACGTGTTATCACAACATCCTTAATTGAAGAAAAAGGCGTAGATGAATTAGAGAAAGCAA
TTGCTGATCTGTTCTTTGAAGGAACAATTGAATCAGCTGATATGACATATGTTTCTAATGCAAGACATATCGGTTTACTA
ACACAAGCAAGAAAAACGATTGGAGATGCAATTGCTGCAATTGAGAACGGTGTTCCAATTGATATGGTTCAAATTGATTT
AACAAGAACATGGGAAATACTTGGTGAGATTACTGGTGATACAGTTCATGAAAGCTTAATTGATCAACTGTTTTCTCAAT
TTTGTTTAGGAAAATAA

Upstream 100 bases:

>100_bases
ATATTCTTTTGTAGATAGGTTCATTATGGTATCCTAATAGTTTAGTGAAGGAAAAAATTATGTTGAAATAGAGAGAAATA
AGCAAGTGAGGTGAAAGGAC

Downstream 100 bases:

>100_bases
TAAAGATCTTTCAAGATGATAGACGATAGTAGGAGGAATAAATAATGGGATACAATGCCGGTTCTTACGATGTCATAGTA
ATCGGTGCAGGCCATGCAGG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MEFDTIAAISTALGEGAIAIVRVSGEDAIEKVNRIFKGKDLTAVSSHTIHYGHIVDLDTNQVIEEVMVSIMRAPKTFTRE
DIVEVNCHGGLVSVNKVLQLILAQGVRLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVAINQMEGRLSKLIG
RLRQEILETLAHVEVNIDYPEYDDVEEMTHRILIEKATHVQNEIEKILETSKQGKILREGIATAIIGRPNVGKSSLLNSL
VQEKKAIVTDIAGTTRDVIEEYVNVRGVPLRLIDTAGIRETEDIVEQIGVERSKEMMSQADLVLIVVNYSEPLTNEDEEL
FRAVQGKDFIVIVNKTDLPQKIEMERVTELASEKRVITTSLIEEKGVDELEKAIADLFFEGTIESADMTYVSNARHIGLL
TQARKTIGDAIAAIENGVPIDMVQIDLTRTWEILGEITGDTVHESLIDQLFSQFCLGK

Sequences:

>Translated_458_residues
MEFDTIAAISTALGEGAIAIVRVSGEDAIEKVNRIFKGKDLTAVSSHTIHYGHIVDLDTNQVIEEVMVSIMRAPKTFTRE
DIVEVNCHGGLVSVNKVLQLILAQGVRLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVAINQMEGRLSKLIG
RLRQEILETLAHVEVNIDYPEYDDVEEMTHRILIEKATHVQNEIEKILETSKQGKILREGIATAIIGRPNVGKSSLLNSL
VQEKKAIVTDIAGTTRDVIEEYVNVRGVPLRLIDTAGIRETEDIVEQIGVERSKEMMSQADLVLIVVNYSEPLTNEDEEL
FRAVQGKDFIVIVNKTDLPQKIEMERVTELASEKRVITTSLIEEKGVDELEKAIADLFFEGTIESADMTYVSNARHIGLL
TQARKTIGDAIAAIENGVPIDMVQIDLTRTWEILGEITGDTVHESLIDQLFSQFCLGK
>Mature_458_residues
MEFDTIAAISTALGEGAIAIVRVSGEDAIEKVNRIFKGKDLTAVSSHTIHYGHIVDLDTNQVIEEVMVSIMRAPKTFTRE
DIVEVNCHGGLVSVNKVLQLILAQGVRLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVAINQMEGRLSKLIG
RLRQEILETLAHVEVNIDYPEYDDVEEMTHRILIEKATHVQNEIEKILETSKQGKILREGIATAIIGRPNVGKSSLLNSL
VQEKKAIVTDIAGTTRDVIEEYVNVRGVPLRLIDTAGIRETEDIVEQIGVERSKEMMSQADLVLIVVNYSEPLTNEDEEL
FRAVQGKDFIVIVNKTDLPQKIEMERVTELASEKRVITTSLIEEKGVDELEKAIADLFFEGTIESADMTYVSNARHIGLL
TQARKTIGDAIAAIENGVPIDMVQIDLTRTWEILGEITGDTVHESLIDQLFSQFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=475, Percent_Identity=30.9473684210526, Blast_Score=205, Evalue=9e-53,
Organism=Homo sapiens, GI193082988, Length=503, Percent_Identity=29.2246520874751, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI193082993, Length=463, Percent_Identity=30.6695464362851, Blast_Score=196, Evalue=6e-50,
Organism=Escherichia coli, GI2367268, Length=463, Percent_Identity=34.341252699784, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI87082120, Length=191, Percent_Identity=32.9842931937173, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17507259, Length=464, Percent_Identity=29.7413793103448, Blast_Score=196, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6323665, Length=501, Percent_Identity=29.940119760479, Blast_Score=205, Evalue=1e-53,
Organism=Drosophila melanogaster, GI45550813, Length=479, Percent_Identity=32.3590814196242, Blast_Score=207, Evalue=8e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50882; Mature: 50882

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFDTIAAISTALGEGAIAIVRVSGEDAIEKVNRIFKGKDLTAVSSHTIHYGHIVDLDTN
CCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCEEECCCEEEECEEEECCHH
QVIEEVMVSIMRAPKTFTREDIVEVNCHGGLVSVNKVLQLILAQGVRLAEPGEFTKRAFL
HHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEHHHHHHHHHHHCCCCCCCCCHHHHHHHH
NGRIDLSQAEAVMDLIRAKTDRAMNVAINQMEGRLSKLIGRLRQEILETLAHVEVNIDYP
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
EYDDVEEMTHRILIEKATHVQNEIEKILETSKQGKILREGIATAIIGRPNVGKSSLLNSL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHH
VQEKKAIVTDIAGTTRDVIEEYVNVRGVPLRLIDTAGIRETEDIVEQIGVERSKEMMSQA
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHCCC
DLVLIVVNYSEPLTNEDEELFRAVQGKDFIVIVNKTDLPQKIEMERVTELASEKRVITTS
CEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHH
LIEEKGVDELEKAIADLFFEGTIESADMTYVSNARHIGLLTQARKTIGDAIAAIENGVPI
HHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCE
DMVQIDLTRTWEILGEITGDTVHESLIDQLFSQFCLGK
EEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEFDTIAAISTALGEGAIAIVRVSGEDAIEKVNRIFKGKDLTAVSSHTIHYGHIVDLDTN
CCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCEEECCCEEEECEEEECCHH
QVIEEVMVSIMRAPKTFTREDIVEVNCHGGLVSVNKVLQLILAQGVRLAEPGEFTKRAFL
HHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEHHHHHHHHHHHCCCCCCCCCHHHHHHHH
NGRIDLSQAEAVMDLIRAKTDRAMNVAINQMEGRLSKLIGRLRQEILETLAHVEVNIDYP
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
EYDDVEEMTHRILIEKATHVQNEIEKILETSKQGKILREGIATAIIGRPNVGKSSLLNSL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHH
VQEKKAIVTDIAGTTRDVIEEYVNVRGVPLRLIDTAGIRETEDIVEQIGVERSKEMMSQA
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHCCC
DLVLIVVNYSEPLTNEDEELFRAVQGKDFIVIVNKTDLPQKIEMERVTELASEKRVITTS
CEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHH
LIEEKGVDELEKAIADLFFEGTIESADMTYVSNARHIGLLTQARKTIGDAIAAIENGVPI
HHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCE
DMVQIDLTRTWEILGEITGDTVHESLIDQLFSQFCLGK
EEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA