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The map label for this gene is phoB [C]

Identifier: 152977462

GI number: 152977462

Start: 3856199

End: 3856870

Strand: Reverse

Name: phoB [C]

Synonym: Bcer98_3794

Alternate gene names: 152977462

Gene position: 3856870-3856199 (Counterclockwise)

Preceding gene: 152977463

Following gene: 152977461

Centisome position: 94.37

GC content: 46.58

Gene sequence:

>672_bases
ATGAAAATTCTCGTGGTGGATGATGAATCGAGCATTCGGAATTTGATTCGGATGCAGCTGGAGATCGAAGGATATGAGGT
ACTGACGGCAGCTAGTGGGAAAGAGGCGCTAGAGAGATGGGAAGATGGGCCGGATGTGCTCATTTTAGATGTGATGCTTC
CGGATATAGATGGATATGAGTTGCTTCGTTTGTTTCGGGAGAGGGATCGCGATATTCCGGTGCTGATGCTGACAGCGAAG
AGTCAGATGAATGATAAGTTGCTTGGGCTTCAGCTTGGTGCAGATGATTATGTGACGAAGCCATTTAATTATGCCGAGCT
GATTCTTCGAGTGAAGAATATGGCGCGGCGCGTGACGAAGAGGGAAGTGCCTCAGAATCATGAGGTGATTCGTGCTGGCG
AGATTGTCGTTTGTCCAAAGGAACGGAAGGTGCATGTGAATGGAGAGGAAGTGCAGCTGACGTACCGGGAGTTTAATTTA
TGTAAGCTGTTTGTTTCGAATCCGCAGCGCGTATTTATGAGAGAAGAATTGCTTGAGAAGGTGTGGGGCTTTGAGTATAT
CGGGAATACAAGAGCGGTGGATATTATGGTGCAGCGCCTTAGAAAGAAACTTAAGGCAAGTGGTGAGCATATTAAGACAA
TTTACGGTGTTGGATATAAGCTGGATTGTTAG

Upstream 100 bases:

>100_bases
AGAAAAAGAGGCGATCATCGACAAAATATGTTCGATGATCGCCTCTCTTTTTTTGTACGATATTTGCTATACTAAAATAT
GCGATGAAAGGAGGAGACAG

Downstream 100 bases:

>100_bases
GTGATGAGATGTCGTTAAAACGAAATATGGTGTTTGGCATTGTCGGATTGTTGATTCCGATTCTTTGTCTTTTATATACA
GTGATTTATATTGCGCTTGA

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MKILVVDDESSIRNLIRMQLEIEGYEVLTAASGKEALERWEDGPDVLILDVMLPDIDGYELLRLFRERDRDIPVLMLTAK
SQMNDKLLGLQLGADDYVTKPFNYAELILRVKNMARRVTKREVPQNHEVIRAGEIVVCPKERKVHVNGEEVQLTYREFNL
CKLFVSNPQRVFMREELLEKVWGFEYIGNTRAVDIMVQRLRKKLKASGEHIKTIYGVGYKLDC

Sequences:

>Translated_223_residues
MKILVVDDESSIRNLIRMQLEIEGYEVLTAASGKEALERWEDGPDVLILDVMLPDIDGYELLRLFRERDRDIPVLMLTAK
SQMNDKLLGLQLGADDYVTKPFNYAELILRVKNMARRVTKREVPQNHEVIRAGEIVVCPKERKVHVNGEEVQLTYREFNL
CKLFVSNPQRVFMREELLEKVWGFEYIGNTRAVDIMVQRLRKKLKASGEHIKTIYGVGYKLDC
>Mature_223_residues
MKILVVDDESSIRNLIRMQLEIEGYEVLTAASGKEALERWEDGPDVLILDVMLPDIDGYELLRLFRERDRDIPVLMLTAK
SQMNDKLLGLQLGADDYVTKPFNYAELILRVKNMARRVTKREVPQNHEVIRAGEIVVCPKERKVHVNGEEVQLTYREFNL
CKLFVSNPQRVFMREELLEKVWGFEYIGNTRAVDIMVQRLRKKLKASGEHIKTIYGVGYKLDC

Specific function: This Protein Is A Positive Regulator For The Phosphate Regulon. Transcription Of This Operon Is Positively Regulated By Phob And Phor When Phosphate Is Limited. [C]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=227, Percent_Identity=36.5638766519824, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1786784, Length=226, Percent_Identity=34.5132743362832, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1790860, Length=223, Percent_Identity=38.1165919282511, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1789809, Length=227, Percent_Identity=34.3612334801762, Blast_Score=131, Evalue=3e-32,
Organism=Escherichia coli, GI1786911, Length=224, Percent_Identity=34.8214285714286, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI1789402, Length=222, Percent_Identity=34.2342342342342, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI2367329, Length=225, Percent_Identity=36.8888888888889, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1788394, Length=224, Percent_Identity=34.8214285714286, Blast_Score=123, Evalue=8e-30,
Organism=Escherichia coli, GI1787375, Length=222, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1790863, Length=228, Percent_Identity=34.6491228070175, Blast_Score=117, Evalue=4e-28,
Organism=Escherichia coli, GI1787229, Length=225, Percent_Identity=33.7777777777778, Blast_Score=113, Evalue=8e-27,
Organism=Escherichia coli, GI87082012, Length=227, Percent_Identity=30.3964757709251, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1790552, Length=222, Percent_Identity=30.6306306306306, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1788550, Length=131, Percent_Identity=32.824427480916, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI145693140, Length=229, Percent_Identity=29.2576419213974, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790299, Length=125, Percent_Identity=34.4, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788713, Length=103, Percent_Identity=37.8640776699029, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788191, Length=111, Percent_Identity=37.8378378378378, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1790437, Length=108, Percent_Identity=36.1111111111111, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1787487, Length=101, Percent_Identity=35.6435643564356, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25927; Mature: 25927

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVVDDESSIRNLIRMQLEIEGYEVLTAASGKEALERWEDGPDVLILDVMLPDIDGYE
CEEEEECCCHHHHHHHHHHEEECCEEEEEECCCHHHHHHCCCCCCEEEEEEECCCCCHHH
LLRLFRERDRDIPVLMLTAKSQMNDKLLGLQLGADDYVTKPFNYAELILRVKNMARRVTK
HHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
REVPQNHEVIRAGEIVVCPKERKVHVNGEEVQLTYREFNLCKLFVSNPQRVFMREELLEK
HCCCCCCCEEEECCEEECCCCCEEEECCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHH
VWGFEYIGNTRAVDIMVQRLRKKLKASGEHIKTIYGVGYKLDC
HCCEECCCCCHHHHHHHHHHHHHHHCCCCCEEHEEECCEEECC
>Mature Secondary Structure
MKILVVDDESSIRNLIRMQLEIEGYEVLTAASGKEALERWEDGPDVLILDVMLPDIDGYE
CEEEEECCCHHHHHHHHHHEEECCEEEEEECCCHHHHHHCCCCCCEEEEEEECCCCCHHH
LLRLFRERDRDIPVLMLTAKSQMNDKLLGLQLGADDYVTKPFNYAELILRVKNMARRVTK
HHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
REVPQNHEVIRAGEIVVCPKERKVHVNGEEVQLTYREFNLCKLFVSNPQRVFMREELLEK
HCCCCCCCEEEECCEEECCCCCEEEECCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHH
VWGFEYIGNTRAVDIMVQRLRKKLKASGEHIKTIYGVGYKLDC
HCCEECCCCCHHHHHHHHHHHHHHHCCCCCEEHEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1554697 [H]