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The map label for this gene is opuD [H]

Identifier: 152977441

GI number: 152977441

Start: 3833804

End: 3835321

Strand: Reverse

Name: opuD [H]

Synonym: Bcer98_3772

Alternate gene names: 152977441

Gene position: 3835321-3833804 (Counterclockwise)

Preceding gene: 152977446

Following gene: 152977434

Centisome position: 93.84

GC content: 38.14

Gene sequence:

>1518_bases
ATGAGGAAACTGACGAAAACATTTATTGTTTCATTGGTATTATGTCTTGCATTTACGATTTGGGGGATTATTCCTGAATC
AGTGATTGGAAAAGGAAGTTTAGGAAATGTCACAACTGCTATTCAGCATGTGTTAGTTAGTAAGTTTGGTTGGTTTTATA
TTATTTCTGTTTCTATTTTCCTAGGGATTTCTATCTTTTTAATCGTTTCAAAATACGGTTCAATTCGTTTAGGGAAAGAT
GATGATGAACCTGATTATAGTTATTTAACATGGTTTGCCATGTTATTTAGTGCTGGTATGGGGATTGGACTGATTTTCTG
GGGCGTTGCGGAACCATTAAATCATTTATATGCACCACCATATGGAGAAAGTGCTACAGAACAAAGTGCCCGTCTGGCAC
TGCGCTTCTCATTTTTCCATTGGGGATTACATCCTTGGGGACTATATGCACTTGTAGCGCTTTGTATTGCGTATTTTACA
TTCCGAAAAGGAAAGCCAAGTACAATTAGTGCGACCGTAGGCTCTTTATTTAAGAGTGGAGAACATGGGCGCGTTGCACA
TATATTTGATGTATTAGCTGTTTTTGCAACAGTGTTTGGTGTTGCAACTTCGTTAGGACTTGGTGCACAACAAATCGCGG
GCGGTATTGGGTATTTAACGCCGATACCGAATTCATTAACAACACAACTCGTGATTATTGGTGCTGTAACAATTCTGTTT
ATGTTATCTGCTCAAACAGGATTGGATAAAGGGATTAAATATTTAAGTAATACGAATATTATTTTAGCCTTTGCACTAAT
GGTAATTCTATTATTTGCAGGTCCAACGAATTTTATTATGAACTATTTCACTTCGACAATTGGGGCTTACATTCAGGAGT
TACCAAGCATGAGTTTTCGATTAAGTCCATTAGATAAAAGCGGAAATCAATGGATTCAATCATGGACAATTTTCTATTGG
GCATGGTGGATTGCATGGTCACCATTCGTTGGGACGTTTATTGCTCGTGTATCGCGCGGACGTACAATTCGAGAGTTTGT
AATTGGTGTACTGCTCGTTCCAACATTAATTGGGGCACTTTGGTTCTCTGTTTTTGGTGGTACTGGTATTCATATGGAAT
TATTTGACCATGCAAATATTTATGAACAAATTAAAGAGATGGGAACAGAAATTGGATTATTCGCTGTGTTCGATCATATG
GGAAGTATCGGTCCTCTGTTATCAGTACTTGCAATTCTCTTGATTTCAACGTTCTTTATTACATCGGCGGATTCTGCGAC
ATTTGTTCTTGGTATGCTAACGACACACGGTAGTTTGAATCCACCAAATCGCATTAAGTTGATTTGGGGTATTGTTCAAG
CGGCGTTAGCAGCAATTTTACTTTATGTCGGTGGTTTAGCTGCATTACAGACAGCTTCTATTATCGCAGCATTTCCATTT
GCATTTGTTATCTTCTTTATGGTAGCTTCGTTATTTAAAGAGCTACAAAAAGAAGGGCGGCATCGCCAACGTAAATGA

Upstream 100 bases:

>100_bases
TGTAGAAAGATACCGGCAGTATTGTTGTAATTTATCCGATTTAACACATTGGAATTCGTGTCATTTTACACAACAAAAGA
AACCTACGAAGGGGGATTTG

Downstream 100 bases:

>100_bases
TCGGAAAAAGGGGCTATCCCAAAAGGGAGTGTCACATAGTCGAAAATTCGACTTATGGGACAGCTCCTTTTTTTACGCAA
AATAATATTGCTCGGCTAAA

Product: choline/carnitine/betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MRKLTKTFIVSLVLCLAFTIWGIIPESVIGKGSLGNVTTAIQHVLVSKFGWFYIISVSIFLGISIFLIVSKYGSIRLGKD
DDEPDYSYLTWFAMLFSAGMGIGLIFWGVAEPLNHLYAPPYGESATEQSARLALRFSFFHWGLHPWGLYALVALCIAYFT
FRKGKPSTISATVGSLFKSGEHGRVAHIFDVLAVFATVFGVATSLGLGAQQIAGGIGYLTPIPNSLTTQLVIIGAVTILF
MLSAQTGLDKGIKYLSNTNIILAFALMVILLFAGPTNFIMNYFTSTIGAYIQELPSMSFRLSPLDKSGNQWIQSWTIFYW
AWWIAWSPFVGTFIARVSRGRTIREFVIGVLLVPTLIGALWFSVFGGTGIHMELFDHANIYEQIKEMGTEIGLFAVFDHM
GSIGPLLSVLAILLISTFFITSADSATFVLGMLTTHGSLNPPNRIKLIWGIVQAALAAILLYVGGLAALQTASIIAAFPF
AFVIFFMVASLFKELQKEGRHRQRK

Sequences:

>Translated_505_residues
MRKLTKTFIVSLVLCLAFTIWGIIPESVIGKGSLGNVTTAIQHVLVSKFGWFYIISVSIFLGISIFLIVSKYGSIRLGKD
DDEPDYSYLTWFAMLFSAGMGIGLIFWGVAEPLNHLYAPPYGESATEQSARLALRFSFFHWGLHPWGLYALVALCIAYFT
FRKGKPSTISATVGSLFKSGEHGRVAHIFDVLAVFATVFGVATSLGLGAQQIAGGIGYLTPIPNSLTTQLVIIGAVTILF
MLSAQTGLDKGIKYLSNTNIILAFALMVILLFAGPTNFIMNYFTSTIGAYIQELPSMSFRLSPLDKSGNQWIQSWTIFYW
AWWIAWSPFVGTFIARVSRGRTIREFVIGVLLVPTLIGALWFSVFGGTGIHMELFDHANIYEQIKEMGTEIGLFAVFDHM
GSIGPLLSVLAILLISTFFITSADSATFVLGMLTTHGSLNPPNRIKLIWGIVQAALAAILLYVGGLAALQTASIIAAFPF
AFVIFFMVASLFKELQKEGRHRQRK
>Mature_505_residues
MRKLTKTFIVSLVLCLAFTIWGIIPESVIGKGSLGNVTTAIQHVLVSKFGWFYIISVSIFLGISIFLIVSKYGSIRLGKD
DDEPDYSYLTWFAMLFSAGMGIGLIFWGVAEPLNHLYAPPYGESATEQSARLALRFSFFHWGLHPWGLYALVALCIAYFT
FRKGKPSTISATVGSLFKSGEHGRVAHIFDVLAVFATVFGVATSLGLGAQQIAGGIGYLTPIPNSLTTQLVIIGAVTILF
MLSAQTGLDKGIKYLSNTNIILAFALMVILLFAGPTNFIMNYFTSTIGAYIQELPSMSFRLSPLDKSGNQWIQSWTIFYW
AWWIAWSPFVGTFIARVSRGRTIREFVIGVLLVPTLIGALWFSVFGGTGIHMELFDHANIYEQIKEMGTEIGLFAVFDHM
GSIGPLLSVLAILLISTFFITSADSATFVLGMLTTHGSLNPPNRIKLIWGIVQAALAAILLYVGGLAALQTASIIAAFPF
AFVIFFMVASLFKELQKEGRHRQRK

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=462, Percent_Identity=36.5800865800866, Blast_Score=331, Evalue=6e-92,
Organism=Escherichia coli, GI1788102, Length=444, Percent_Identity=29.5045045045045, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1786224, Length=469, Percent_Identity=28.7846481876333, Blast_Score=201, Evalue=9e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 55450; Mature: 55450

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLTKTFIVSLVLCLAFTIWGIIPESVIGKGSLGNVTTAIQHVLVSKFGWFYIISVSIF
CCHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGISIFLIVSKYGSIRLGKDDDEPDYSYLTWFAMLFSAGMGIGLIFWGVAEPLNHLYAPP
HHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
YGESATEQSARLALRFSFFHWGLHPWGLYALVALCIAYFTFRKGKPSTISATVGSLFKSG
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
EHGRVAHIFDVLAVFATVFGVATSLGLGAQQIAGGIGYLTPIPNSLTTQLVIIGAVTILF
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
MLSAQTGLDKGIKYLSNTNIILAFALMVILLFAGPTNFIMNYFTSTIGAYIQELPSMSFR
HHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEE
LSPLDKSGNQWIQSWTIFYWAWWIAWSPFVGTFIARVSRGRTIREFVIGVLLVPTLIGAL
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
WFSVFGGTGIHMELFDHANIYEQIKEMGTEIGLFAVFDHMGSIGPLLSVLAILLISTFFI
HHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TSADSATFVLGMLTTHGSLNPPNRIKLIWGIVQAALAAILLYVGGLAALQTASIIAAFPF
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFVIFFMVASLFKELQKEGRHRQRK
HHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]