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The map label for this gene is celB [H]

Identifier: 152977423

GI number: 152977423

Start: 3809712

End: 3811019

Strand: Reverse

Name: celB [H]

Synonym: Bcer98_3753

Alternate gene names: 152977423

Gene position: 3811019-3809712 (Counterclockwise)

Preceding gene: 152977424

Following gene: 152977422

Centisome position: 93.25

GC content: 37.69

Gene sequence:

>1308_bases
ATGCAAAAGTTTATTGCATTTATGGAGAAGTATATTGTTCCAGTTGCTGGTAGAATCGGATCGCAACGCCATTTAGCAGC
GATTCGTGATGGATTTATTGCGGTTATGCCATTGATTTTAGTGGGGGCGCTTGCATCACTTGTGAATGGTTTTCCATCTG
AAGTTTTCCAAAATTTTATGAAAGATACATTTGGTGAAGTTTGGAAAGATGTTGGGGGCGGAATGTGGACTGGTTCATTT
GCAATTTTGGCGCTGAGTGTAGCATTTACGACAGCTTATAACTTAGGGAAATCTTATAATGTGGATGGATTGTCAGCAGG
TCTTATCTCATTTTGTTCTTTAATTATTCTGACTCCCGTAACTCCAAAAGAAGGCGGTTTGAACTTCCTTTGGACAGGTA
CTCAAGGTTTATTCGTAGCAATTATTGTTGCACTTCTTGTAACGGAAGTATTTCGCTTCTTTGTACAAAGAGAAATCACT
CTTAAAATGCCAGATGGGGTACCACCAGCGGTTATTCGTTCTTTTGCCGCATTAGTTCCAGCATTTGTTATTTTAACAGT
TGTATCATTTATTGCATTAGCAGTGAAATTAGCAGGTACAAGTGTTCATGAGTTCATCTTCCATACACTTCAAGTACCAT
TACAAAACTTAGCAGGTTCATTACCAAGTGCAATTATTGTTGTTTTATTTGTTCATGTACTTTGGTTCTTTGGATTACAT
GGCCCAAATATTGTGGCAGGTATTATTGAGCCATTGTATTTACCGGCATTAGAGGAAAATGTTAAATTATTCCGAGATGG
CGTATCTGCTTTTGATGTTCCGAACATTGTTACAAAGTCATTCTTTGATACTTTCGTATATCTTGGTGGTTCCGGAGCAA
CATTAGCATTCTTAGTTGTTGTACTAATTGTCGCAAAAAGCGCACAATTACGTGGTGTATCTCGTCTGTCAATTGGACCG
GGTGCATTTAATATTAACGAACCAGTTATTTTTGGTACACCAATTATTTTAAATCCAATTTTGTTCGTTCCGTTTATTGT
TACACCAATTGTACTTGTTATTACTTCTTATACAGCTATTTATTTCGGATGGGTACCAAAAACAGTTACAATTATTCCTT
GGGCAACACCACCAGTTATTAGTGGTTACCTTGTAACAGGGGGACATATTTCTGGTGCAATTCTACAATTATTTAACTTT
GTTCTTGCGATGGTGATTTACTACCCATTCGTTGTATTATGCGATCGCTCTGTTGTACGCACTGAAAAGGCAGCAGCGCA
AGGAAACGATCAATCTGTACCTATGTAA

Upstream 100 bases:

>100_bases
GAATCGATGTAATTAATCCAATGCACTACGGCATGATGAATGGAGAAGCAGTATTAGATCAAGCGTTAACACTTAAAAAA
TAATTAGGGAGGGAAATGAA

Downstream 100 bases:

>100_bases
TCATGATTGTTCTCACAGGTTCATATAGTCTGTGAGAACAATTACTTTCTAAACAAGAAAACATCTGGAAGAACATTAAA
ATAAAATAATAACAATGTAA

Product: PTS system lactose/cellobiose family IIC subunit

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIC-Cel; PTS system cellobiose-specific EIIC component [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MQKFIAFMEKYIVPVAGRIGSQRHLAAIRDGFIAVMPLILVGALASLVNGFPSEVFQNFMKDTFGEVWKDVGGGMWTGSF
AILALSVAFTTAYNLGKSYNVDGLSAGLISFCSLIILTPVTPKEGGLNFLWTGTQGLFVAIIVALLVTEVFRFFVQREIT
LKMPDGVPPAVIRSFAALVPAFVILTVVSFIALAVKLAGTSVHEFIFHTLQVPLQNLAGSLPSAIIVVLFVHVLWFFGLH
GPNIVAGIIEPLYLPALEENVKLFRDGVSAFDVPNIVTKSFFDTFVYLGGSGATLAFLVVVLIVAKSAQLRGVSRLSIGP
GAFNINEPVIFGTPIILNPILFVPFIVTPIVLVITSYTAIYFGWVPKTVTIIPWATPPVISGYLVTGGHISGAILQLFNF
VLAMVIYYPFVVLCDRSVVRTEKAAAQGNDQSVPM

Sequences:

>Translated_435_residues
MQKFIAFMEKYIVPVAGRIGSQRHLAAIRDGFIAVMPLILVGALASLVNGFPSEVFQNFMKDTFGEVWKDVGGGMWTGSF
AILALSVAFTTAYNLGKSYNVDGLSAGLISFCSLIILTPVTPKEGGLNFLWTGTQGLFVAIIVALLVTEVFRFFVQREIT
LKMPDGVPPAVIRSFAALVPAFVILTVVSFIALAVKLAGTSVHEFIFHTLQVPLQNLAGSLPSAIIVVLFVHVLWFFGLH
GPNIVAGIIEPLYLPALEENVKLFRDGVSAFDVPNIVTKSFFDTFVYLGGSGATLAFLVVVLIVAKSAQLRGVSRLSIGP
GAFNINEPVIFGTPIILNPILFVPFIVTPIVLVITSYTAIYFGWVPKTVTIIPWATPPVISGYLVTGGHISGAILQLFNF
VLAMVIYYPFVVLCDRSVVRTEKAAAQGNDQSVPM
>Mature_435_residues
MQKFIAFMEKYIVPVAGRIGSQRHLAAIRDGFIAVMPLILVGALASLVNGFPSEVFQNFMKDTFGEVWKDVGGGMWTGSF
AILALSVAFTTAYNLGKSYNVDGLSAGLISFCSLIILTPVTPKEGGLNFLWTGTQGLFVAIIVALLVTEVFRFFVQREIT
LKMPDGVPPAVIRSFAALVPAFVILTVVSFIALAVKLAGTSVHEFIFHTLQVPLQNLAGSLPSAIIVVLFVHVLWFFGLH
GPNIVAGIIEPLYLPALEENVKLFRDGVSAFDVPNIVTKSFFDTFVYLGGSGATLAFLVVVLIVAKSAQLRGVSRLSIGP
GAFNINEPVIFGTPIILNPILFVPFIVTPIVLVITSYTAIYFGWVPKTVTIIPWATPPVISGYLVTGGHISGAILQLFNF
VLAMVIYYPFVVLCDRSVVRTEKAAAQGNDQSVPM

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=453, Percent_Identity=34.4370860927152, Blast_Score=271, Evalue=7e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 46918; Mature: 46918

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCCCCCCCC
>Mature Secondary Structure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HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8407820 [H]