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The map label for this gene is cwlH [H]

Identifier: 152977137

GI number: 152977137

Start: 3509903

End: 3512434

Strand: Reverse

Name: cwlH [H]

Synonym: Bcer98_3449

Alternate gene names: 152977137

Gene position: 3512434-3509903 (Counterclockwise)

Preceding gene: 152977138

Following gene: 152977134

Centisome position: 85.94

GC content: 35.07

Gene sequence:

>2532_bases
ATGACCGCAACACCTGCATTTGCAGCTTCAGCTTCCTCTGTAAAACAATTAGATGAAGCAATATCTTATGATATACAGGT
GCTGAAAAGTATAGAAGAACATATGAAAGATGAAGATGGAAAAGGTGAAAAAATAGGAGAAAATGGTGAAATACAAGGCG
ATTTTCTTGTATATATTCCAAAAGAAATGCCTATATATTCTTCTTATGATTTAAATACTCCAATTATACATCAAGTATCT
CAACAGGTAGTAAAAGCAACTAAGAAAAAAGAAAACTGGTATTATATTGAAACTGATTCTGTAAAAGGTTGGGTACAAAA
TAAAGAGAATGTTCTTGAAGAACGAGAAGTATATTCTTTACCGAAAGATACAAAAAAAATACAAATTAAAGAAGTAACAT
CTGTATATTCAGCACCATTTCATGCATTCAAAGAGAATGATGTTTTACAACCTCAATCTGTTGAGGCGATTACACAAGCT
GGAGAATGGTTTCAAATTCAAACGTCTAATGGGATAAAGTGGATTCATTCGTCATCTGCAAAATTTGAAGGAACAAAGGC
CTCTCTTATTCAGTCTACAGTCAATTATAAAGAAGATGCTTCTCTTCAATACGCGCCATCAAGCTTAAATGTGGGGAATA
TATATGGTGTGAAGTTTACTGAATGGATTGTTCCAAAAGGAAATCCACAAATTCGCCCAGGTTATGCTATGGACCCTCGT
TATATCACAATCCACGAAACTGCTAATGAAAGAAGAGGTGCGGATGCAAAAAATCATGCTAAATATTTGATGAATCAAGC
GACAGGAAGTACAAGTCGCGGTGCATCATGGCATTTTACAGTAGATGACAAAGAGATTTACCAACATTTACCTTTAAATG
AAAATGGATGGCATGCAGGTGATGGAACAAACGGGACTGGAAATAGACAATCAATTGGAATTGAAATTGCTGTAAATCAG
GATGGAAACTATGAGAAAGCAGTAGAAAATGCTCGCAAATTGACAGCTTATTTAATGCAGGAATTAAATATTCCAATAGA
TAATGTAAAGAAACATCAACATTGGAGTGGAAAAAATTGTCCAGCAAAAATAATTGCAAGAGGGACATGGCCAGCTTTTT
TAAATGGTGCGAAAAGTTATTATGAAAAAAATAAGCCCGCTGTAATCTCAAATAAAGAAGAGGTTCACCGAAAAGACGAT
ATTTCTGGGAAATGGTATGAACACGAAATTCGTGAGCTCGCGAAAAAAGGTATTATGGCGGGAGATGGAAAGGGCTCATA
TTGGCCAGAGCGTCTTGTAACACGTGCAGAATTTGCTGCTTTAATTACACGTTCTTTACAATTACCAGAAGGGAAATCGA
ATTTTAAGGATCTTAGTTTAGCTCATCCATCATTAGTAGATGGCATAAACCGAGCAGCGGCAGCTGGTATCATTCATGGT
CGAGGCAATGGTATATTTGCACCAAACGATACGATTACGCGTGAAGAAGTAGTCATTATGGTAGATCGTGCTTTGCAAAG
CAAAGGAATTACTGGAGCATTAAAAGAAGCTCCATTTATAGATCAAGATGCAGCTTATGATAAAAAAGCGCTGCAACGTG
TATATGGATTAGGAATTGTTAAAGGAAATGATCAAAATCAATTCTTACCAAAAGGAACGGCAAGCCGCGCGGAAGCAGCA
GCATTTTTAAATCGTATGTTACGTGTAATGGATAAAAAGCCAGGAGAAAATGCGAAACACAATATTACTGCTAAATCATA
TGTAGAATTAGATTTAACATTAGCTTCCAATATTACAGGTAAAGAGATTGATAACTTTATTGCAAGATACCATCCTGATA
GCCCATTAGTAGGACATGGACAAGACTTTATTGAAGCGCAAAATAATCATGGGGTGAATGCATTATATTTAGCTGCACAT
GCTATTTTGGAATCGGGTTATGGAAAATCTGAAATTGCATATCGAAAACACAACTTATTTGGATTACGTGCATATGATTG
GGATCCATTTGGAAATGCGAAATATTTACAAAGTTATGGAGAAAGTATTGCTTATAATGCAAATTATGTACGTGCGCGTT
ACTTAGAAAAAACAGGAATGTATTATAATGGCCCAACGTTAACGGGTATGAATGTTAAGTATGCAACAGATAAAGAATGG
GCTCCTAAAATTGCAAATATTATGGAGCGTATTAAGCCTTTTAAGGCAGAAGACTATATGTATGCAAAAAGGCTTTCAAA
AAATCCAAGTATATTAAATGTTGATGCGTTACCATCGGCAATTCCATATAAGTTGTATCCAGCTGGGACAAAAGCTACGG
TAAAATCATCAGCACATTATTATCAAATACCATTTCCATTCAATGGTAAAATTAGAAGTGAAAATATTACTCCAGAACAA
AATAAGGTGGGTAGATTGTCTACTGGATCGATTGTAAATATTTATCGTGAAGATCCAAATGGATGGATTGAGTTTTCGTT
TGATACTAGCAATCAAAAATATTGGACAGAAAAAAATAATTTAAATTTATGA

Upstream 100 bases:

>100_bases
AGGTATCAAACACGAAATAAATAGGATATTTCGTGTAACAATGATGATAAAAAAGAAGATTTTAAATGTTTTAGCGATGA
CAGTGGTACTTCAGGGAGGA

Downstream 100 bases:

>100_bases
TAAATAGTTTAATAGTATTAAAAAAAGCAGATTGAATCTTAATCTGTACCCTTTGTAAAGGACATTTTAAAAAAAGTTAG
GCAGCCGCGAGAAGATGATT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 843; Mature: 842

Protein sequence:

>843_residues
MTATPAFAASASSVKQLDEAISYDIQVLKSIEEHMKDEDGKGEKIGENGEIQGDFLVYIPKEMPIYSSYDLNTPIIHQVS
QQVVKATKKKENWYYIETDSVKGWVQNKENVLEEREVYSLPKDTKKIQIKEVTSVYSAPFHAFKENDVLQPQSVEAITQA
GEWFQIQTSNGIKWIHSSSAKFEGTKASLIQSTVNYKEDASLQYAPSSLNVGNIYGVKFTEWIVPKGNPQIRPGYAMDPR
YITIHETANERRGADAKNHAKYLMNQATGSTSRGASWHFTVDDKEIYQHLPLNENGWHAGDGTNGTGNRQSIGIEIAVNQ
DGNYEKAVENARKLTAYLMQELNIPIDNVKKHQHWSGKNCPAKIIARGTWPAFLNGAKSYYEKNKPAVISNKEEVHRKDD
ISGKWYEHEIRELAKKGIMAGDGKGSYWPERLVTRAEFAALITRSLQLPEGKSNFKDLSLAHPSLVDGINRAAAAGIIHG
RGNGIFAPNDTITREEVVIMVDRALQSKGITGALKEAPFIDQDAAYDKKALQRVYGLGIVKGNDQNQFLPKGTASRAEAA
AFLNRMLRVMDKKPGENAKHNITAKSYVELDLTLASNITGKEIDNFIARYHPDSPLVGHGQDFIEAQNNHGVNALYLAAH
AILESGYGKSEIAYRKHNLFGLRAYDWDPFGNAKYLQSYGESIAYNANYVRARYLEKTGMYYNGPTLTGMNVKYATDKEW
APKIANIMERIKPFKAEDYMYAKRLSKNPSILNVDALPSAIPYKLYPAGTKATVKSSAHYYQIPFPFNGKIRSENITPEQ
NKVGRLSTGSIVNIYREDPNGWIEFSFDTSNQKYWTEKNNLNL

Sequences:

>Translated_843_residues
MTATPAFAASASSVKQLDEAISYDIQVLKSIEEHMKDEDGKGEKIGENGEIQGDFLVYIPKEMPIYSSYDLNTPIIHQVS
QQVVKATKKKENWYYIETDSVKGWVQNKENVLEEREVYSLPKDTKKIQIKEVTSVYSAPFHAFKENDVLQPQSVEAITQA
GEWFQIQTSNGIKWIHSSSAKFEGTKASLIQSTVNYKEDASLQYAPSSLNVGNIYGVKFTEWIVPKGNPQIRPGYAMDPR
YITIHETANERRGADAKNHAKYLMNQATGSTSRGASWHFTVDDKEIYQHLPLNENGWHAGDGTNGTGNRQSIGIEIAVNQ
DGNYEKAVENARKLTAYLMQELNIPIDNVKKHQHWSGKNCPAKIIARGTWPAFLNGAKSYYEKNKPAVISNKEEVHRKDD
ISGKWYEHEIRELAKKGIMAGDGKGSYWPERLVTRAEFAALITRSLQLPEGKSNFKDLSLAHPSLVDGINRAAAAGIIHG
RGNGIFAPNDTITREEVVIMVDRALQSKGITGALKEAPFIDQDAAYDKKALQRVYGLGIVKGNDQNQFLPKGTASRAEAA
AFLNRMLRVMDKKPGENAKHNITAKSYVELDLTLASNITGKEIDNFIARYHPDSPLVGHGQDFIEAQNNHGVNALYLAAH
AILESGYGKSEIAYRKHNLFGLRAYDWDPFGNAKYLQSYGESIAYNANYVRARYLEKTGMYYNGPTLTGMNVKYATDKEW
APKIANIMERIKPFKAEDYMYAKRLSKNPSILNVDALPSAIPYKLYPAGTKATVKSSAHYYQIPFPFNGKIRSENITPEQ
NKVGRLSTGSIVNIYREDPNGWIEFSFDTSNQKYWTEKNNLNL
>Mature_842_residues
TATPAFAASASSVKQLDEAISYDIQVLKSIEEHMKDEDGKGEKIGENGEIQGDFLVYIPKEMPIYSSYDLNTPIIHQVSQ
QVVKATKKKENWYYIETDSVKGWVQNKENVLEEREVYSLPKDTKKIQIKEVTSVYSAPFHAFKENDVLQPQSVEAITQAG
EWFQIQTSNGIKWIHSSSAKFEGTKASLIQSTVNYKEDASLQYAPSSLNVGNIYGVKFTEWIVPKGNPQIRPGYAMDPRY
ITIHETANERRGADAKNHAKYLMNQATGSTSRGASWHFTVDDKEIYQHLPLNENGWHAGDGTNGTGNRQSIGIEIAVNQD
GNYEKAVENARKLTAYLMQELNIPIDNVKKHQHWSGKNCPAKIIARGTWPAFLNGAKSYYEKNKPAVISNKEEVHRKDDI
SGKWYEHEIRELAKKGIMAGDGKGSYWPERLVTRAEFAALITRSLQLPEGKSNFKDLSLAHPSLVDGINRAAAAGIIHGR
GNGIFAPNDTITREEVVIMVDRALQSKGITGALKEAPFIDQDAAYDKKALQRVYGLGIVKGNDQNQFLPKGTASRAEAAA
FLNRMLRVMDKKPGENAKHNITAKSYVELDLTLASNITGKEIDNFIARYHPDSPLVGHGQDFIEAQNNHGVNALYLAAHA
ILESGYGKSEIAYRKHNLFGLRAYDWDPFGNAKYLQSYGESIAYNANYVRARYLEKTGMYYNGPTLTGMNVKYATDKEWA
PKIANIMERIKPFKAEDYMYAKRLSKNPSILNVDALPSAIPYKLYPAGTKATVKSSAHYYQIPFPFNGKIRSENITPEQN
KVGRLSTGSIVNIYREDPNGWIEFSFDTSNQKYWTEKNNLNL

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 94548; Mature: 94417

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATPAFAASASSVKQLDEAISYDIQVLKSIEEHMKDEDGKGEKIGENGEIQGDFLVYIP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEC
KEMPIYSSYDLNTPIIHQVSQQVVKATKKKENWYYIETDSVKGWVQNKENVLEEREVYSL
CCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCHHHHHHHHHHHCC
PKDTKKIQIKEVTSVYSAPFHAFKENDVLQPQSVEAITQAGEWFQIQTSNGIKWIHSSSA
CCCCCEEEHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHCCCCEEEEEECCCEEEEECCCC
KFEGTKASLIQSTVNYKEDASLQYAPSSLNVGNIYGVKFTEWIVPKGNPQIRPGYAMDPR
CCCCHHHHHHHHHHCCCCCCCEEECCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCE
YITIHETANERRGADAKNHAKYLMNQATGSTSRGASWHFTVDDKEIYQHLPLNENGWHAG
EEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHCCCCCCCCCCC
DGTNGTGNRQSIGIEIAVNQDGNYEKAVENARKLTAYLMQELNIPIDNVKKHQHWSGKNC
CCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCC
PAKIIARGTWPAFLNGAKSYYEKNKPAVISNKEEVHRKDDISGKWYEHEIRELAKKGIMA
CCEEEECCCCHHHHHHHHHHHHCCCCEEECCHHHHHHHCCCCCCHHHHHHHHHHHCCCEE
GDGKGSYWPERLVTRAEFAALITRSLQLPEGKSNFKDLSLAHPSLVDGINRAAAAGIIHG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEE
RGNGIFAPNDTITREEVVIMVDRALQSKGITGALKEAPFIDQDAAYDKKALQRVYGLGIV
CCCEEECCCCCCCCCCEEEEEEHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHHCEEE
KGNDQNQFLPKGTASRAEAAAFLNRMLRVMDKKPGENAKHNITAKSYVELDLTLASNITG
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEECCCCH
KEIDNFIARYHPDSPLVGHGQDFIEAQNNHGVNALYLAAHAILESGYGKSEIAYRKHNLF
HHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHEEECCCEE
GLRAYDWDPFGNAKYLQSYGESIAYNANYVRARYLEKTGMYYNGPTLTGMNVKYATDKEW
EEEEECCCCCCCHHHHHHCCCCEEECCCHHHHHHHHHCCCEECCCEEECCEEEECCCCCC
APKIANIMERIKPFKAEDYMYAKRLSKNPSILNVDALPSAIPYKLYPAGTKATVKSSAHY
CHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCEEEECCCCCCEEECCCEE
YQIPFPFNGKIRSENITPEQNKVGRLSTGSIVNIYREDPNGWIEFSFDTSNQKYWTEKNN
EEECCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCEEEECCCC
LNL
CCC
>Mature Secondary Structure 
TATPAFAASASSVKQLDEAISYDIQVLKSIEEHMKDEDGKGEKIGENGEIQGDFLVYIP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEC
KEMPIYSSYDLNTPIIHQVSQQVVKATKKKENWYYIETDSVKGWVQNKENVLEEREVYSL
CCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCHHHHHHHHHHHCC
PKDTKKIQIKEVTSVYSAPFHAFKENDVLQPQSVEAITQAGEWFQIQTSNGIKWIHSSSA
CCCCCEEEHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHCCCCEEEEEECCCEEEEECCCC
KFEGTKASLIQSTVNYKEDASLQYAPSSLNVGNIYGVKFTEWIVPKGNPQIRPGYAMDPR
CCCCHHHHHHHHHHCCCCCCCEEECCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCE
YITIHETANERRGADAKNHAKYLMNQATGSTSRGASWHFTVDDKEIYQHLPLNENGWHAG
EEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHCCCCCCCCCCC
DGTNGTGNRQSIGIEIAVNQDGNYEKAVENARKLTAYLMQELNIPIDNVKKHQHWSGKNC
CCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCC
PAKIIARGTWPAFLNGAKSYYEKNKPAVISNKEEVHRKDDISGKWYEHEIRELAKKGIMA
CCEEEECCCCHHHHHHHHHHHHCCCCEEECCHHHHHHHCCCCCCHHHHHHHHHHHCCCEE
GDGKGSYWPERLVTRAEFAALITRSLQLPEGKSNFKDLSLAHPSLVDGINRAAAAGIIHG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEE
RGNGIFAPNDTITREEVVIMVDRALQSKGITGALKEAPFIDQDAAYDKKALQRVYGLGIV
CCCEEECCCCCCCCCCEEEEEEHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHHCEEE
KGNDQNQFLPKGTASRAEAAAFLNRMLRVMDKKPGENAKHNITAKSYVELDLTLASNITG
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEECCCCH
KEIDNFIARYHPDSPLVGHGQDFIEAQNNHGVNALYLAAHAILESGYGKSEIAYRKHNLF
HHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHEEECCCEE
GLRAYDWDPFGNAKYLQSYGESIAYNANYVRARYLEKTGMYYNGPTLTGMNVKYATDKEW
EEEEECCCCCCCHHHHHHCCCCEEECCCHHHHHHHHHCCCEECCCEEECCEEEECCCCCC
APKIANIMERIKPFKAEDYMYAKRLSKNPSILNVDALPSAIPYKLYPAGTKATVKSSAHY
CHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCEEEECCCCCCEEECCCEE
YQIPFPFNGKIRSENITPEQNKVGRLSTGSIVNIYREDPNGWIEFSFDTSNQKYWTEKNN
EEECCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCEEEECCCC
LNL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]