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The map label for this gene is leuS [H]

Identifier: 152977105

GI number: 152977105

Start: 3478317

End: 3480725

Strand: Reverse

Name: leuS [H]

Synonym: Bcer98_3415

Alternate gene names: 152977105

Gene position: 3480725-3478317 (Counterclockwise)

Preceding gene: 152977106

Following gene: 152977104

Centisome position: 85.17

GC content: 39.77

Gene sequence:

>2409_bases
ATGAGCTTTAATCATCAAGAAATTGAGAAGAAGTGGCAAGCGTATTGGGAAGAGAATAAAACATTCCGTACGCCAGATGA
GACAGACAAACCAAAATTTTATGCATTAGATATGTTCCCATATCCATCAGGTGCAGGTCTTCACGTAGGACATCCAGAGG
GATATACAGCAACAGATATTTTATCACGTATGAAACGTATGCAAGGGTATAATGTTCTTCATCCAATGGGATGGGATGCA
TTCGGTCTTCCAGCAGAGCAATATGCACTTGATACGGGGAATAGCCCAGCGGAATTTACAGAGAAAAATATTAACACATT
CCGTAATCAAATTAAGTCACTTGGCTTCTCATACGATTGGGATCGCGAAGTAAATACAACAGATCCGAATTACTATAAAT
GGACACAATGGATTTTCTTAAAGCTATTTGAAAAAGGTTTAGCTTACGTGGATGAAATTCCTGTAAACTGGTGCCCAGCA
CTTGGTACAGTGCTTGCGAATGAAGAAGTAATTGATGGTAAGAGTGAGCGCGGTGGACACCCTGTTGAGCGCCGTCCGAT
GAAACAATGGATGCTAAAAATTACTGCATATGCAGATCGCTTACTAGAAGACTTAGATGAGCTTGATTGGCCAGAAAGTT
TAAAAGATATGCAACGCAACTGGATTGGACGTTCTGAAGGTGCAGAAGTACATTTTAATATCGACGGTACAGATAAGAAA
TTTACAATCTTTACAACGCGCCCTGATACATTATTTGGAGCAACTTACTGCGTACTTGCTCCAGAGCATGCACTTGTTGC
AGAAATTACAACAGAAGATCAAAAAGGAGCTGTAGAAGCTTACATTGATGTTGTAAAATCGAAAAGTGATTTAGAACGTA
CAGAGCTTGCGAAAGAAAAAACAGGTGTATTCACGGGTGCATACGCAATCAATCCTGTAAACGGAGAGAAATTACCAATT
TGGATTGCTGATTATGTGCTTGCTAGCTATGGAACAGGTGCTGTAATGGCAGTTCCAGCTCATGATGAACGCGATTATGA
GTTTGCCAAAACTTTTGATCTGCCAATGAAAGAAGTGGTAAAAGGCGGAGACATTACGAAAGAAGCATACACAGCTGATG
GGGAACACATCGATTCTGCATTCCTTAATGGTTTAAATAAGGAAGAGGCAATTGCAAAAATGATTGAATGGCTTGAAGTA
ACAGGCGCAGGAAATCAAAAAGTAACGTACCGCCTTCGTGACTGGTTATTTAGTCGTCAACGTTATTGGGGTGAGCCAAT
TCCAATTATCCATTGGGAAGATGGTACGATGACAGCGGTGAAAGAAGAAGAATTACCGTTAGTTCTTCCAAAAACAGATA
ATATTCGTCCGTCTGGAACAGGTGAATCACCACTTGCAAACATCGATGAGTGGGTAAATGTTGTAGATCCAGAGACTGGT
AAAAAAGGCCGTCGTGAAACAAATACAATGCCGCAATGGGCTGGTAGCTGCTGGTACTATTTACGTTATATCGATCCAAA
CAATAACGAAGCACTTGTAGATCCTGAAAAAGCAAAACAATGGCTTCCAGTTGATATTTATATTGGCGGGGCAGAGCATG
CTGTACTTCATTTACTATATGCTCGTTTCTGGCATAAAGTATTATATGATATCGGTGTTGTTCCAACGAAAGAGCCATTC
CAACAACTATTTAACCAAGGTATGATTTTAGGAGAAAACAACGAGAAAATGAGTAAGTCAAAAGGTAACGTTGTAAACCC
TGATGACATCGTAGCAAGCCATGGTGCTGATACACTTCGTCTTTACGAAATGTTCATGGGACCATTAGATGCTTCAATCG
CTTGGTCTGAAAACGGCCTTGATGGAGCGCGTCGTTTCTTAGATCGCGTATGGCGCCTATTCGTTCAAGAAAACGGTGAA
TTAAGCGAGAAAATTACAGATGCGCCGAATAAAGAGCTTGAAAAAGCATATCACCAAACAGTGAAGAAAGTAACAGAAGA
CTATGAAGAGCTTCATTTTAATACTGCAATTTCTCAAATGATGATGTTTATTAATGATGCATATAAAGCGGAAACGCTTC
CGAAAGAATATGTAGAAGGTTTCGTGAAGTTACTTGCACCAGTTGCACCGCACATTGCAGAGGAACTTTGGAGCAAGCTT
GGTTATAATGAAACAATCACATATGCAAGCTGGCCAACATTTGATGAGTCTAAACTTGTAGAAGATGAAGTAGAAATTGT
TGTTCAAGTAATGGGAAAAGTTCGTGCAAAACTGAAAATGAAAAAAGACGCATCAAAAGAAGAAATGGAACAACTTGCAC
TTGAAGAAGTGAAAGAACAAATTGAAGGGAAAACAGTTCGCAAAGTAATTGTCGTTCCTGGAAAACTTGTTAATATTGTT
GCAAACTGA

Upstream 100 bases:

>100_bases
GGTACCGCGGGAATCTATAACCTCTCGTCCCTTTTTAGGGATGAGAGGTTTTTTGTATTTTAGGCGGTGAAAATAAATTG
TATTTTAAGGAGGGAATCTC

Downstream 100 bases:

>100_bases
TGAAAAAGAGCAGTCCTTTATAAGAAGGCTGCTCTTTTTCATAGTGGAAAAAAGATGAATTCCTATACAGACAAACACCT
ATTCAATTGTGTGCAATATG

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 802; Mature: 801

Protein sequence:

>802_residues
MSFNHQEIEKKWQAYWEENKTFRTPDETDKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRMKRMQGYNVLHPMGWDA
FGLPAEQYALDTGNSPAEFTEKNINTFRNQIKSLGFSYDWDREVNTTDPNYYKWTQWIFLKLFEKGLAYVDEIPVNWCPA
LGTVLANEEVIDGKSERGGHPVERRPMKQWMLKITAYADRLLEDLDELDWPESLKDMQRNWIGRSEGAEVHFNIDGTDKK
FTIFTTRPDTLFGATYCVLAPEHALVAEITTEDQKGAVEAYIDVVKSKSDLERTELAKEKTGVFTGAYAINPVNGEKLPI
WIADYVLASYGTGAVMAVPAHDERDYEFAKTFDLPMKEVVKGGDITKEAYTADGEHIDSAFLNGLNKEEAIAKMIEWLEV
TGAGNQKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTMTAVKEEELPLVLPKTDNIRPSGTGESPLANIDEWVNVVDPETG
KKGRRETNTMPQWAGSCWYYLRYIDPNNNEALVDPEKAKQWLPVDIYIGGAEHAVLHLLYARFWHKVLYDIGVVPTKEPF
QQLFNQGMILGENNEKMSKSKGNVVNPDDIVASHGADTLRLYEMFMGPLDASIAWSENGLDGARRFLDRVWRLFVQENGE
LSEKITDAPNKELEKAYHQTVKKVTEDYEELHFNTAISQMMMFINDAYKAETLPKEYVEGFVKLLAPVAPHIAEELWSKL
GYNETITYASWPTFDESKLVEDEVEIVVQVMGKVRAKLKMKKDASKEEMEQLALEEVKEQIEGKTVRKVIVVPGKLVNIV
AN

Sequences:

>Translated_802_residues
MSFNHQEIEKKWQAYWEENKTFRTPDETDKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRMKRMQGYNVLHPMGWDA
FGLPAEQYALDTGNSPAEFTEKNINTFRNQIKSLGFSYDWDREVNTTDPNYYKWTQWIFLKLFEKGLAYVDEIPVNWCPA
LGTVLANEEVIDGKSERGGHPVERRPMKQWMLKITAYADRLLEDLDELDWPESLKDMQRNWIGRSEGAEVHFNIDGTDKK
FTIFTTRPDTLFGATYCVLAPEHALVAEITTEDQKGAVEAYIDVVKSKSDLERTELAKEKTGVFTGAYAINPVNGEKLPI
WIADYVLASYGTGAVMAVPAHDERDYEFAKTFDLPMKEVVKGGDITKEAYTADGEHIDSAFLNGLNKEEAIAKMIEWLEV
TGAGNQKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTMTAVKEEELPLVLPKTDNIRPSGTGESPLANIDEWVNVVDPETG
KKGRRETNTMPQWAGSCWYYLRYIDPNNNEALVDPEKAKQWLPVDIYIGGAEHAVLHLLYARFWHKVLYDIGVVPTKEPF
QQLFNQGMILGENNEKMSKSKGNVVNPDDIVASHGADTLRLYEMFMGPLDASIAWSENGLDGARRFLDRVWRLFVQENGE
LSEKITDAPNKELEKAYHQTVKKVTEDYEELHFNTAISQMMMFINDAYKAETLPKEYVEGFVKLLAPVAPHIAEELWSKL
GYNETITYASWPTFDESKLVEDEVEIVVQVMGKVRAKLKMKKDASKEEMEQLALEEVKEQIEGKTVRKVIVVPGKLVNIV
AN
>Mature_801_residues
SFNHQEIEKKWQAYWEENKTFRTPDETDKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRMKRMQGYNVLHPMGWDAF
GLPAEQYALDTGNSPAEFTEKNINTFRNQIKSLGFSYDWDREVNTTDPNYYKWTQWIFLKLFEKGLAYVDEIPVNWCPAL
GTVLANEEVIDGKSERGGHPVERRPMKQWMLKITAYADRLLEDLDELDWPESLKDMQRNWIGRSEGAEVHFNIDGTDKKF
TIFTTRPDTLFGATYCVLAPEHALVAEITTEDQKGAVEAYIDVVKSKSDLERTELAKEKTGVFTGAYAINPVNGEKLPIW
IADYVLASYGTGAVMAVPAHDERDYEFAKTFDLPMKEVVKGGDITKEAYTADGEHIDSAFLNGLNKEEAIAKMIEWLEVT
GAGNQKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTMTAVKEEELPLVLPKTDNIRPSGTGESPLANIDEWVNVVDPETGK
KGRRETNTMPQWAGSCWYYLRYIDPNNNEALVDPEKAKQWLPVDIYIGGAEHAVLHLLYARFWHKVLYDIGVVPTKEPFQ
QLFNQGMILGENNEKMSKSKGNVVNPDDIVASHGADTLRLYEMFMGPLDASIAWSENGLDGARRFLDRVWRLFVQENGEL
SEKITDAPNKELEKAYHQTVKKVTEDYEELHFNTAISQMMMFINDAYKAETLPKEYVEGFVKLLAPVAPHIAEELWSKLG
YNETITYASWPTFDESKLVEDEVEIVVQVMGKVRAKLKMKKDASKEEMEQLALEEVKEQIEGKTVRKVIVVPGKLVNIVA
N

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=874, Percent_Identity=31.9221967963387, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI94721239, Length=181, Percent_Identity=28.1767955801105, Blast_Score=71, Evalue=4e-12,
Organism=Homo sapiens, GI94721241, Length=181, Percent_Identity=28.1767955801105, Blast_Score=71, Evalue=4e-12,
Organism=Escherichia coli, GI1786861, Length=862, Percent_Identity=43.8515081206497, Blast_Score=696, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=391, Percent_Identity=25.3196930946292, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI2367096, Length=343, Percent_Identity=23.6151603498542, Blast_Score=63, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71997510, Length=803, Percent_Identity=30.0124533001245, Blast_Score=325, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI71997517, Length=771, Percent_Identity=30.609597924773, Blast_Score=324, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI212645227, Length=366, Percent_Identity=29.2349726775956, Blast_Score=136, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17510661, Length=450, Percent_Identity=24.4444444444444, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17554638, Length=283, Percent_Identity=26.1484098939929, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71980946, Length=154, Percent_Identity=30.5194805194805, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323414, Length=889, Percent_Identity=35.8830146231721, Blast_Score=474, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6321531, Length=423, Percent_Identity=24.5862884160757, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319395, Length=373, Percent_Identity=23.8605898123324, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21355409, Length=834, Percent_Identity=32.2541966426858, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI281366294, Length=904, Percent_Identity=22.787610619469, Blast_Score=120, Evalue=4e-27,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 91594; Mature: 91462

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFNHQEIEKKWQAYWEENKTFRTPDETDKPKFYALDMFPYPSGAGLHVGHPEGYTATDI
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCEECCCCCCCCHHHH
LSRMKRMQGYNVLHPMGWDAFGLPAEQYALDTGNSPAEFTEKNINTFRNQIKSLGFSYDW
HHHHHHHCCCCCCCCCCCCCCCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
DREVNTTDPNYYKWTQWIFLKLFEKGLAYVDEIPVNWCPALGTVLANEEVIDGKSERGGH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCCCCCCCC
PVERRPMKQWMLKITAYADRLLEDLDELDWPESLKDMQRNWIGRSEGAEVHFNIDGTDKK
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCE
FTIFTTRPDTLFGATYCVLAPEHALVAEITTEDQKGAVEAYIDVVKSKSDLERTELAKEK
EEEEECCCCHHHCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHH
TGVFTGAYAINPVNGEKLPIWIADYVLASYGTGAVMAVPAHDERDYEFAKTFDLPMKEVV
CCEEEEEEEECCCCCCCCEEHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHCCCCHHHHH
KGGDITKEAYTADGEHIDSAFLNGLNKEEAIAKMIEWLEVTGAGNQKVTYRLRDWLFSRQ
CCCCCCHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
RYWGEPIPIIHWEDGTMTAVKEEELPLVLPKTDNIRPSGTGESPLANIDEWVNVVDPETG
HHCCCCCCEEEECCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
KKGRRETNTMPQWAGSCWYYLRYIDPNNNEALVDPEKAKQWLPVDIYIGGAEHAVLHLLY
CCCCCCCCCCCHHCCCEEEEEEEECCCCCCEEECHHHHCCCCCEEEEECCHHHHHHHHHH
ARFWHKVLYDIGVVPTKEPFQQLFNQGMILGENNEKMSKSKGNVVNPDDIVASHGADTLR
HHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCHHHHCCCCCCCHHHHHHCCCCHHHH
LYEMFMGPLDASIAWSENGLDGARRFLDRVWRLFVQENGELSEKITDAPNKELEKAYHQT
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHH
VKKVTEDYEELHFNTAISQMMMFINDAYKAETLPKEYVEGFVKLLAPVAPHIAEELWSKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GYNETITYASWPTFDESKLVEDEVEIVVQVMGKVRAKLKMKKDASKEEMEQLALEEVKEQ
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IEGKTVRKVIVVPGKLVNIVAN
HCCCCEEEEEECCCHHHHEECC
>Mature Secondary Structure 
SFNHQEIEKKWQAYWEENKTFRTPDETDKPKFYALDMFPYPSGAGLHVGHPEGYTATDI
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCEECCCCCCCCHHHH
LSRMKRMQGYNVLHPMGWDAFGLPAEQYALDTGNSPAEFTEKNINTFRNQIKSLGFSYDW
HHHHHHHCCCCCCCCCCCCCCCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
DREVNTTDPNYYKWTQWIFLKLFEKGLAYVDEIPVNWCPALGTVLANEEVIDGKSERGGH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCCCCCCCC
PVERRPMKQWMLKITAYADRLLEDLDELDWPESLKDMQRNWIGRSEGAEVHFNIDGTDKK
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCE
FTIFTTRPDTLFGATYCVLAPEHALVAEITTEDQKGAVEAYIDVVKSKSDLERTELAKEK
EEEEECCCCHHHCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHH
TGVFTGAYAINPVNGEKLPIWIADYVLASYGTGAVMAVPAHDERDYEFAKTFDLPMKEVV
CCEEEEEEEECCCCCCCCEEHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHCCCCHHHHH
KGGDITKEAYTADGEHIDSAFLNGLNKEEAIAKMIEWLEVTGAGNQKVTYRLRDWLFSRQ
CCCCCCHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
RYWGEPIPIIHWEDGTMTAVKEEELPLVLPKTDNIRPSGTGESPLANIDEWVNVVDPETG
HHCCCCCCEEEECCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
KKGRRETNTMPQWAGSCWYYLRYIDPNNNEALVDPEKAKQWLPVDIYIGGAEHAVLHLLY
CCCCCCCCCCCHHCCCEEEEEEEECCCCCCEEECHHHHCCCCCEEEEECCHHHHHHHHHH
ARFWHKVLYDIGVVPTKEPFQQLFNQGMILGENNEKMSKSKGNVVNPDDIVASHGADTLR
HHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCHHHHCCCCCCCHHHHHHCCCCHHHH
LYEMFMGPLDASIAWSENGLDGARRFLDRVWRLFVQENGELSEKITDAPNKELEKAYHQT
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHH
VKKVTEDYEELHFNTAISQMMMFINDAYKAETLPKEYVEGFVKLLAPVAPHIAEELWSKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GYNETITYASWPTFDESKLVEDEVEIVVQVMGKVRAKLKMKKDASKEEMEQLALEEVKEQ
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IEGKTVRKVIVVPGKLVNIVAN
HCCCCEEEEEECCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA