Click here to switch to the map view.

The map label for this gene is ytzG [H]

Identifier: 152977062

GI number: 152977062

Start: 3437715

End: 3438443

Strand: Reverse

Name: ytzG [H]

Synonym: Bcer98_3369

Alternate gene names: 152977062

Gene position: 3438443-3437715 (Counterclockwise)

Preceding gene: 152977063

Following gene: 152977060

Centisome position: 84.13

GC content: 34.29

Gene sequence:

>729_bases
ATGAGATTAGATAAATTATTAGCAAATATGGGATATGGAAGTAGAAAAGAAGTAAAAAAATTATTGAAAGACGGCGTTGT
AAGGATTGATGGAAAACCAGTCAAAGATGCAAAGTTTCATGTGAATATAGAAAAGCAAGAAGTAACAATTTATGGTGAAG
TTGTTGAATATAAAGAGTTTGTTTATTTAATGATGCATAAACCACAAGGAGTCATTTCTGCAACAGAAGACGATCATCAT
GAAACAGTTGTTGATCTACTGGAATTTGAAGATGCAATCTTTAATCCATTTCCTGTTGGAAGACTTGATATTGATACAGA
AGGTTTGCTGCTATTAACAAATGACGGAAAGTTGACGCATCAGCTTCTATCACCGAAAAAGCATGTGCCAAAAAAATATT
ATGCACATATAGAAGGTGTAGTTACAGAAAAAGATGTGGAAGATTTTGCAAAAGGTGTTGTTCTAGATGATGGCTATGAA
ACAAAACCTGGCGAACTGACCATTTTAAACAGTGATGATATATCTGAAATTGAGCTTGTAATTACAGAAGGTAAATTCCA
TCAGGTAAAGCGTATGTTTGAAGCTGTTGGGAAAAAAGTCGTCTACTTGAAGAGAGTAGAGATGGGACCTTTAACGTTAG
ATGAGGAATTAGAACTTGGGCAATACCGTGAGTTGACAGATGAAGAGGTTGAAATGTTAAAAATGTATCAAGTGCAACAC
GAGAATTAA

Upstream 100 bases:

>100_bases
ATTAGAAAAGATTCAACGTAAACTTGGCAGTAAATTAAAAATAAAAATGAAATCAAAAGGGGCGTAAGTAGCGTCCCTTT
TCAATAAAGAAGGTGATTGA

Downstream 100 bases:

>100_bases
GAGTGATACCGATTTCAAGGTGCAGAAAGAACCCCTTTCTATTCATTTCGAAAGGGGTATTTATTTTACATATAATTGTT
TTCGTTATGAGGTCATTCTC

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MRLDKLLANMGYGSRKEVKKLLKDGVVRIDGKPVKDAKFHVNIEKQEVTIYGEVVEYKEFVYLMMHKPQGVISATEDDHH
ETVVDLLEFEDAIFNPFPVGRLDIDTEGLLLLTNDGKLTHQLLSPKKHVPKKYYAHIEGVVTEKDVEDFAKGVVLDDGYE
TKPGELTILNSDDISEIELVITEGKFHQVKRMFEAVGKKVVYLKRVEMGPLTLDEELELGQYRELTDEEVEMLKMYQVQH
EN

Sequences:

>Translated_242_residues
MRLDKLLANMGYGSRKEVKKLLKDGVVRIDGKPVKDAKFHVNIEKQEVTIYGEVVEYKEFVYLMMHKPQGVISATEDDHH
ETVVDLLEFEDAIFNPFPVGRLDIDTEGLLLLTNDGKLTHQLLSPKKHVPKKYYAHIEGVVTEKDVEDFAKGVVLDDGYE
TKPGELTILNSDDISEIELVITEGKFHQVKRMFEAVGKKVVYLKRVEMGPLTLDEELELGQYRELTDEEVEMLKMYQVQH
EN
>Mature_242_residues
MRLDKLLANMGYGSRKEVKKLLKDGVVRIDGKPVKDAKFHVNIEKQEVTIYGEVVEYKEFVYLMMHKPQGVISATEDDHH
ETVVDLLEFEDAIFNPFPVGRLDIDTEGLLLLTNDGKLTHQLLSPKKHVPKKYYAHIEGVVTEKDVEDFAKGVVLDDGYE
TKPGELTILNSDDISEIELVITEGKFHQVKRMFEAVGKKVVYLKRVEMGPLTLDEELELGQYRELTDEEVEMLKMYQVQH
EN

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=231, Percent_Identity=38.961038961039, Blast_Score=157, Evalue=5e-40,
Organism=Escherichia coli, GI1787524, Length=244, Percent_Identity=31.1475409836066, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790453, Length=242, Percent_Identity=28.5123966942149, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI87081838, Length=181, Percent_Identity=32.5966850828729, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27791; Mature: 27791

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKLLANMGYGSRKEVKKLLKDGVVRIDGKPVKDAKFHVNIEKQEVTIYGEVVEYKEF
CCHHHHHHHCCCCCHHHHHHHHHCCEEEECCCCCCCCEEEEEEEEEEEEEEHHHHHHHHH
VYLMMHKPQGVISATEDDHHETVVDLLEFEDAIFNPFPVGRLDIDTEGLLLLTNDGKLTH
EEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCHHH
QLLSPKKHVPKKYYAHIEGVVTEKDVEDFAKGVVLDDGYETKPGELTILNSDDISEIELV
HHHCCHHHCCHHHHHHHHCEECCCCHHHHHCCEEEECCCCCCCCEEEEECCCCCCEEEEE
ITEGKFHQVKRMFEAVGKKVVYLKRVEMGPLTLDEELELGQYRELTDEEVEMLKMYQVQH
EECCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHC
EN
CC
>Mature Secondary Structure
MRLDKLLANMGYGSRKEVKKLLKDGVVRIDGKPVKDAKFHVNIEKQEVTIYGEVVEYKEF
CCHHHHHHHCCCCCHHHHHHHHHCCEEEECCCCCCCCEEEEEEEEEEEEEEHHHHHHHHH
VYLMMHKPQGVISATEDDHHETVVDLLEFEDAIFNPFPVGRLDIDTEGLLLLTNDGKLTH
EEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCHHH
QLLSPKKHVPKKYYAHIEGVVTEKDVEDFAKGVVLDDGYETKPGELTILNSDDISEIELV
HHHCCHHHCCHHHHHHHHCEECCCCHHHHHCCEEEECCCCCCCCEEEEECCCCCCEEEEE
ITEGKFHQVKRMFEAVGKKVVYLKRVEMGPLTLDEELELGQYRELTDEEVEMLKMYQVQH
EECCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHC
EN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]