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The map label for this gene is ligT [C]

Identifier: 152977059

GI number: 152977059

Start: 3434978

End: 3435532

Strand: Reverse

Name: ligT [C]

Synonym: Bcer98_3366

Alternate gene names: 152977059

Gene position: 3435532-3434978 (Counterclockwise)

Preceding gene: 152977060

Following gene: 152977058

Centisome position: 84.06

GC content: 36.58

Gene sequence:

>555_bases
ATGGTACCACATTATTTTATAGCAATTACATTACCGCATGATATAAAAGAACGATTGGCAAATTATAAGGAAGAAATGAA
CGATACGTTACCATTTCGCTCATGGGTACATAAAGAAGACTATCATATTACACTTGCCTTTTTAGGCAGTGCAACGCAAG
AGCAGTTACAAGAGATAAAAGAGGGATTGCATCTTCTTAAAAATGAACAGGAATTTTCACTTTTATTACAAGGGTTTTCT
ACCTTTGGTCCCCCAGAACGTCCACGCATATTTTGGGCTGGATTAGAAGAAAGTAAAGCATTACTTCATTTACAAAAGAA
AGTGCATAGCATTTGTGAACGGAGTGGTTTTTCACTTGAAACGCGGCCCTTTCACCCGCATATTACTATTGCGCGTAAAT
GGATCGGAGAGGCGATGTTTCATATAGAGGATATAAAGGAATTAAAACCAGTATCATTTCCAGTAGATACTGTGACACTA
TATAGATCGAATATGAAAGATACGCCTAAATATCACCCTGTTACAGAAATAAAGTTGCAGAAATGTAATGCATGA

Upstream 100 bases:

>100_bases
TACGAAGTAACCGCCCTTCATTTGAAGGGCGGGCTTCTTTTTCATGATTGAATGAGATAGGAAAGAAGAGAAACAATAAT
ACAAAGGGGGAAGATACGAT

Downstream 100 bases:

>100_bases
AAACGTTTTTTTTGCCGGTGAATATACTTATAGAAATGAATAAATTGGTGTATGCAGGATTACTCAATAGATGAATCTTT
AATTTGGAGAATGATGGTGA

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MVPHYFIAITLPHDIKERLANYKEEMNDTLPFRSWVHKEDYHITLAFLGSATQEQLQEIKEGLHLLKNEQEFSLLLQGFS
TFGPPERPRIFWAGLEESKALLHLQKKVHSICERSGFSLETRPFHPHITIARKWIGEAMFHIEDIKELKPVSFPVDTVTL
YRSNMKDTPKYHPVTEIKLQKCNA

Sequences:

>Translated_184_residues
MVPHYFIAITLPHDIKERLANYKEEMNDTLPFRSWVHKEDYHITLAFLGSATQEQLQEIKEGLHLLKNEQEFSLLLQGFS
TFGPPERPRIFWAGLEESKALLHLQKKVHSICERSGFSLETRPFHPHITIARKWIGEAMFHIEDIKELKPVSFPVDTVTL
YRSNMKDTPKYHPVTEIKLQKCNA
>Mature_184_residues
MVPHYFIAITLPHDIKERLANYKEEMNDTLPFRSWVHKEDYHITLAFLGSATQEQLQEIKEGLHLLKNEQEFSLLLQGFS
TFGPPERPRIFWAGLEESKALLHLQKKVHSICERSGFSLETRPFHPHITIARKWIGEAMFHIEDIKELKPVSFPVDTVTL
YRSNMKDTPKYHPVTEIKLQKCNA

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 21521; Mature: 21521

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPHYFIAITLPHDIKERLANYKEEMNDTLPFRSWVHKEDYHITLAFLGSATQEQLQEIK
CCCEEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEEEEECCCCCHHHHHHHH
EGLHLLKNEQEFSLLLQGFSTFGPPERPRIFWAGLEESKALLHLQKKVHSICERSGFSLE
HHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCC
TRPFHPHITIARKWIGEAMFHIEDIKELKPVSFPVDTVTLYRSNMKDTPKYHPVTEIKLQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCHHEEEE
KCNA
CCCC
>Mature Secondary Structure
MVPHYFIAITLPHDIKERLANYKEEMNDTLPFRSWVHKEDYHITLAFLGSATQEQLQEIK
CCCEEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEEEEECCCCCHHHHHHHH
EGLHLLKNEQEFSLLLQGFSTFGPPERPRIFWAGLEESKALLHLQKKVHSICERSGFSLE
HHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCC
TRPFHPHITIARKWIGEAMFHIEDIKELKPVSFPVDTVTLYRSNMKDTPKYHPVTEIKLQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCHHEEEE
KCNA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8190087 [H]