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The map label for this gene is amyX [H]

Identifier: 152977058

GI number: 152977058

Start: 3432733

End: 3434871

Strand: Reverse

Name: amyX [H]

Synonym: Bcer98_3365

Alternate gene names: 152977058

Gene position: 3434871-3432733 (Counterclockwise)

Preceding gene: 152977059

Following gene: 152977057

Centisome position: 84.04

GC content: 36.19

Gene sequence:

>2139_bases
ATGTTGAGAGTAGCACGTCCGTTTGAAGCGTATTTAGATGAAATGAATAAGATTACAATTTTACTCCCCTACACATATGG
AACAAGCCAGACTTTTTCCTTAAAGGAAGAAAATGAAATGAGAGAACTCTCCATTGTTAAAACGACCTTACTTCCTAATG
CAACGAAATATGAATGTTATACAGAGGAGGTATTAGATGTAGGGAAATATTATACAGTGCGGGATGAACGGGATATAGAA
ACGGATTTGCAAATTGGAGCTGTGATTCGCACAGCTGCTTTTGATGAAACATATTTTTACGAAGGTACAGATTTAGGAGC
AAATTATAAAAAGGAAGAAACGGTTTTTAAAGTATGGGCTCCAACAGCGAGATTTGCTAAGGTTCGAATTTATAGAGAGA
ATGAAGAGTATACAGATTATGAAATGAAACGAGGAGATAAGGGAGTTTGGTCTTGTATTCTCCAAGAAGACTTGGAAGGA
GCGCAGTATACATTTCTCGTTTGTATTAACTTAGTATGGAATGAAGCGGTGGATCCATATGCAAAGTCGGTATCGGAAAA
TGGACAATATGGAATTGTTATCAATATGGAAAAAGTCTCTGTCACAAAATATACATTGCCTAAGTTCCCTTCTTCTGTAG
ATGCCATACTGTATGAAATGCATATTCGCGATATGACTATGCATCCAAATAGTGGTGTAAAGAGAAAAGGGATGTATAAA
GGGCTTATGGAAGAAAATACAAAAGGGAAACATGGAACGCTAACGGCTTTAGCTCATGTGAAAGATTTAGGTGTTACGCA
TGTTGAAATATTGCCATTACATCATTTTGCAGGTGTAGATGAGATGACTCCCTTTGCTGCATATAATTGGGGATATAATC
CTCTGTATTACAATGTTCCGACAGGAGTGTATGCAACAAATCCGTCTGATCCATATAATCGCATGTTAGAGTGTAAACAA
TTAATTGAAACATTTCATAAGCATGGGATTCGCGTAATTATTGATGTTGTATACAATCATGTATATAAAAGAGAAACATC
ATCGTTTGAAAAGCTTGTACCAGGTTATTATTTTCGACACGATAAAAATGGAATGCCGTCAAATGGAACTGGAGTTGGAA
ATGACTTAGCATCTGAGCGGAAAATGATGAGGAAATTTATTGTAGATTCTGTTTTATATTGGTTAACTGAATATAATGTT
GACGGGTTTCGTTTTGATTTAATGGGAATTTTAGATGTAGAAACGATGAATCAAATTGAACAGGAAGTGCGAAAAAAGAA
ACAAGATGCAATCTTGTTAGGAGAGGGTTGGGATTTAGAAACACCGCTTGCAGCAGAAGAAAAAGCGATGTTACGTAATG
CTAGTAAACTTCCGCATATTGCACAATTTAATGATCAATTTCGTGATTGGATAAAAGGGAGCACTTTTGACGTAAGGAAA
AGAGGTTTTGCATTTGGAGGACAAGTAGGAGAAAGTGACTTGCAATATGTACTACAAGGGAGTTTGTCCAGTGTAAAAGA
ACAAGGTCTTTTTTTGGAACCGACTCAAAGTGTAAACTATGTGGAATGTCATGATAATATGACGATGTGGGATAAACTCG
TACGCAGTAATCAAGAAGCGGAAGTGATTTTAAAGCGTCGTCATCGTTTAGCGACAGCGATGGTTCTTTTCTCACAAGGG
ATTCCGTTTTTACACGCAGGGCAAGAATTTTATCGTTCCAAAAAAGGGAATGAAAACAGTTATAATGCTCCGGATGAGAT
TAATCAAATGGATTGGACTCAAAAAGAGAAAGAATTGGTGACTGTTGATTATATAAAAGGATTAATCGCAATCAGGAAAG
AACATGGTGCATTTCGTTTTCAATCAGCCGACTTAATTCAAAAGCATATGAAATTCTTTCCAACACCTTCTTCTGTCATT
GCTTATCATTTACAAAAAGTAGAATCATTTGGACCATGGAAAGAAATTATCGTTTTATTTCATAATGGTCTACAAATAGA
AACAGTTATACTCCCAAAACAAGAAACATGGCACGTATTTGCAAACGAGAATGAAGCAAAAGCACAAGCAATTTCTTCAT
TTGAAGGAAGGGAACTGCAAATGGCACCTATTAGCACGTATATATTGGTGAAAATGTGA

Upstream 100 bases:

>100_bases
TTTTTTTGCCGGTGAATATACTTATAGAAATGAATAAATTGGTGTATGCAGGATTACTCAATAGATGAATCTTTAATTTG
GAGAATGATGGTGATTTTTT

Downstream 100 bases:

>100_bases
CAAATCGCTACTTGACGATGAGAATGTAATAAATGTAGAATTGACAAAGGTGACGATTTGAATAAGTTGTCATTCTTATA
TAGGTTTTGTAAAATAAAGT

Product: pullulanase, type I

Products: NA

Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]

Number of amino acids: Translated: 712; Mature: 712

Protein sequence:

>712_residues
MLRVARPFEAYLDEMNKITILLPYTYGTSQTFSLKEENEMRELSIVKTTLLPNATKYECYTEEVLDVGKYYTVRDERDIE
TDLQIGAVIRTAAFDETYFYEGTDLGANYKKEETVFKVWAPTARFAKVRIYRENEEYTDYEMKRGDKGVWSCILQEDLEG
AQYTFLVCINLVWNEAVDPYAKSVSENGQYGIVINMEKVSVTKYTLPKFPSSVDAILYEMHIRDMTMHPNSGVKRKGMYK
GLMEENTKGKHGTLTALAHVKDLGVTHVEILPLHHFAGVDEMTPFAAYNWGYNPLYYNVPTGVYATNPSDPYNRMLECKQ
LIETFHKHGIRVIIDVVYNHVYKRETSSFEKLVPGYYFRHDKNGMPSNGTGVGNDLASERKMMRKFIVDSVLYWLTEYNV
DGFRFDLMGILDVETMNQIEQEVRKKKQDAILLGEGWDLETPLAAEEKAMLRNASKLPHIAQFNDQFRDWIKGSTFDVRK
RGFAFGGQVGESDLQYVLQGSLSSVKEQGLFLEPTQSVNYVECHDNMTMWDKLVRSNQEAEVILKRRHRLATAMVLFSQG
IPFLHAGQEFYRSKKGNENSYNAPDEINQMDWTQKEKELVTVDYIKGLIAIRKEHGAFRFQSADLIQKHMKFFPTPSSVI
AYHLQKVESFGPWKEIIVLFHNGLQIETVILPKQETWHVFANENEAKAQAISSFEGRELQMAPISTYILVKM

Sequences:

>Translated_712_residues
MLRVARPFEAYLDEMNKITILLPYTYGTSQTFSLKEENEMRELSIVKTTLLPNATKYECYTEEVLDVGKYYTVRDERDIE
TDLQIGAVIRTAAFDETYFYEGTDLGANYKKEETVFKVWAPTARFAKVRIYRENEEYTDYEMKRGDKGVWSCILQEDLEG
AQYTFLVCINLVWNEAVDPYAKSVSENGQYGIVINMEKVSVTKYTLPKFPSSVDAILYEMHIRDMTMHPNSGVKRKGMYK
GLMEENTKGKHGTLTALAHVKDLGVTHVEILPLHHFAGVDEMTPFAAYNWGYNPLYYNVPTGVYATNPSDPYNRMLECKQ
LIETFHKHGIRVIIDVVYNHVYKRETSSFEKLVPGYYFRHDKNGMPSNGTGVGNDLASERKMMRKFIVDSVLYWLTEYNV
DGFRFDLMGILDVETMNQIEQEVRKKKQDAILLGEGWDLETPLAAEEKAMLRNASKLPHIAQFNDQFRDWIKGSTFDVRK
RGFAFGGQVGESDLQYVLQGSLSSVKEQGLFLEPTQSVNYVECHDNMTMWDKLVRSNQEAEVILKRRHRLATAMVLFSQG
IPFLHAGQEFYRSKKGNENSYNAPDEINQMDWTQKEKELVTVDYIKGLIAIRKEHGAFRFQSADLIQKHMKFFPTPSSVI
AYHLQKVESFGPWKEIIVLFHNGLQIETVILPKQETWHVFANENEAKAQAISSFEGRELQMAPISTYILVKM
>Mature_712_residues
MLRVARPFEAYLDEMNKITILLPYTYGTSQTFSLKEENEMRELSIVKTTLLPNATKYECYTEEVLDVGKYYTVRDERDIE
TDLQIGAVIRTAAFDETYFYEGTDLGANYKKEETVFKVWAPTARFAKVRIYRENEEYTDYEMKRGDKGVWSCILQEDLEG
AQYTFLVCINLVWNEAVDPYAKSVSENGQYGIVINMEKVSVTKYTLPKFPSSVDAILYEMHIRDMTMHPNSGVKRKGMYK
GLMEENTKGKHGTLTALAHVKDLGVTHVEILPLHHFAGVDEMTPFAAYNWGYNPLYYNVPTGVYATNPSDPYNRMLECKQ
LIETFHKHGIRVIIDVVYNHVYKRETSSFEKLVPGYYFRHDKNGMPSNGTGVGNDLASERKMMRKFIVDSVLYWLTEYNV
DGFRFDLMGILDVETMNQIEQEVRKKKQDAILLGEGWDLETPLAAEEKAMLRNASKLPHIAQFNDQFRDWIKGSTFDVRK
RGFAFGGQVGESDLQYVLQGSLSSVKEQGLFLEPTQSVNYVECHDNMTMWDKLVRSNQEAEVILKRRHRLATAMVLFSQG
IPFLHAGQEFYRSKKGNENSYNAPDEINQMDWTQKEKELVTVDYIKGLIAIRKEHGAFRFQSADLIQKHMKFFPTPSSVI
AYHLQKVESFGPWKEIIVLFHNGLQIETVILPKQETWHVFANENEAKAQAISSFEGRELQMAPISTYILVKM

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=673, Percent_Identity=23.1797919762259, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789839, Length=323, Percent_Identity=23.2198142414861, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR011840 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.41 [H]

Molecular weight: Translated: 82145; Mature: 82145

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRVARPFEAYLDEMNKITILLPYTYGTSQTFSLKEENEMRELSIVKTTLLPNATKYECY
CCCCCCHHHHHHHCCCCEEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCHHH
TEEVLDVGKYYTVRDERDIETDLQIGAVIRTAAFDETYFYEGTDLGANYKKEETVFKVWA
HHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEEEC
PTARFAKVRIYRENEEYTDYEMKRGDKGVWSCILQEDLEGAQYTFLVCINLVWNEAVDPY
CCCCEEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCHH
AKSVSENGQYGIVINMEKVSVTKYTLPKFPSSVDAILYEMHIRDMTMHPNSGVKRKGMYK
HHHHCCCCCEEEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHEEECCCCCCCHHHHHH
GLMEENTKGKHGTLTALAHVKDLGVTHVEILPLHHFAGVDEMTPFAAYNWGYNPLYYNVP
HHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHCCCCCCCCCEEECCCCCCEEEECC
TGVYATNPSDPYNRMLECKQLIETFHKHGIRVIIDVVYNHVYKRETSSFEKLVPGYYFRH
CCEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEEEE
DKNGMPSNGTGVGNDLASERKMMRKFIVDSVLYWLTEYNVDGFRFDLMGILDVETMNQIE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHHHH
QEVRKKKQDAILLGEGWDLETPLAAEEKAMLRNASKLPHIAQFNDQFRDWIKGSTFDVRK
HHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHH
RGFAFGGQVGESDLQYVLQGSLSSVKEQGLFLEPTQSVNYVECHDNMTMWDKLVRSNQEA
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEECCCHHHHHHHHHCCCHH
EVILKRRHRLATAMVLFSQGIPFLHAGQEFYRSKKGNENSYNAPDEINQMDWTQKEKELV
HHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHCCCCCCCCCCCHHCCCCCCCCHHHCEE
TVDYIKGLIAIRKEHGAFRFQSADLIQKHMKFFPTPSSVIAYHLQKVESFGPWKEIIVLF
EHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHEE
HNGLQIETVILPKQETWHVFANENEAKAQAISSFEGRELQMAPISTYILVKM
ECCCEEEEEEECCCCCEEEEECCCHHHHHHHHCCCCCEEEEECCEEEEEEEC
>Mature Secondary Structure
MLRVARPFEAYLDEMNKITILLPYTYGTSQTFSLKEENEMRELSIVKTTLLPNATKYECY
CCCCCCHHHHHHHCCCCEEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCHHH
TEEVLDVGKYYTVRDERDIETDLQIGAVIRTAAFDETYFYEGTDLGANYKKEETVFKVWA
HHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEEEC
PTARFAKVRIYRENEEYTDYEMKRGDKGVWSCILQEDLEGAQYTFLVCINLVWNEAVDPY
CCCCEEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCHH
AKSVSENGQYGIVINMEKVSVTKYTLPKFPSSVDAILYEMHIRDMTMHPNSGVKRKGMYK
HHHHCCCCCEEEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHEEECCCCCCCHHHHHH
GLMEENTKGKHGTLTALAHVKDLGVTHVEILPLHHFAGVDEMTPFAAYNWGYNPLYYNVP
HHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHCCCCCCCCCEEECCCCCCEEEECC
TGVYATNPSDPYNRMLECKQLIETFHKHGIRVIIDVVYNHVYKRETSSFEKLVPGYYFRH
CCEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEEEE
DKNGMPSNGTGVGNDLASERKMMRKFIVDSVLYWLTEYNVDGFRFDLMGILDVETMNQIE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHHHH
QEVRKKKQDAILLGEGWDLETPLAAEEKAMLRNASKLPHIAQFNDQFRDWIKGSTFDVRK
HHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHH
RGFAFGGQVGESDLQYVLQGSLSSVKEQGLFLEPTQSVNYVECHDNMTMWDKLVRSNQEA
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEECCCHHHHHHHHHCCCHH
EVILKRRHRLATAMVLFSQGIPFLHAGQEFYRSKKGNENSYNAPDEINQMDWTQKEKELV
HHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHCCCCCCCCCCCHHCCCCCCCCHHHCEE
TVDYIKGLIAIRKEHGAFRFQSADLIQKHMKFFPTPSSVIAYHLQKVESFGPWKEIIVLF
EHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHEE
HNGLQIETVILPKQETWHVFANENEAKAQAISSFEGRELQMAPISTYILVKM
ECCCEEEEEEECCCCCEEEEECCCHHHHHHHHCCCCCEEEEECCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]