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The map label for this gene is trmB

Identifier: 152977055

GI number: 152977055

Start: 3430537

End: 3431190

Strand: Reverse

Name: trmB

Synonym: Bcer98_3362

Alternate gene names: 152977055

Gene position: 3431190-3430537 (Counterclockwise)

Preceding gene: 152977057

Following gene: 152977054

Centisome position: 83.95

GC content: 34.4

Gene sequence:

>654_bases
ATGCGTTTAAGACATAAACCGTATGCAATGGATCGCATTAAAGAATATTCACAATTTGTAATTGGAAACCCTGAGGAACA
TCGCGGCAACTGGAAAGAACTTTTTGGAAATGATCATCCTATTCATATTGAAGTAGGGACAGGACGCGGACGTTTTGTTT
ATGAAATGGCAAAAGCAAACCCGGATATTAACTACATCGGAATTGAAAAGTTTACGAGTGTCATTGTAGATGCTCTTGAT
AAGTTAATTGAAAAAGAATTACCAAATTTAAAGTTAATTAATAAGGATGCAGAAGATTTAACAGTTTTCTTTACAAAAGG
TGAAATTGACCGAGTATATTTAAATTTCTCCGATCCATGGCCGAAGAAACGGCATGCAAAGCGCCGTTTAACATATAAAA
CATTTTTACGTAATTATGAAGAAGTGTTAGTAAAAGATGGAGAAATTCACTTTAAAACAGATAACCAAGCTCTATTCGAA
TACTCTCTTATGAGTATGGCTGAATATGGGATGGTATTTACATTCTTAAGTCTCGACCTTCATAATAGTGACTTTGAAGG
AAATATTATGACAGAGTATGAAGAAAAGTTCTCTAGTAAAGGTCATCGTATTTATCGAGTAGAAGCAAAATATCGTACGG
AGCCTGTGCAGTAA

Upstream 100 bases:

>100_bases
GAAACGGACCGAGTGCTCGTACGTACTTGGTTTTTTATTTGACAGTCTACTGAGAAAAGAAAGAAGGAAAAAATAGTCTA
ATAAAGAGAGGGAAACATCT

Downstream 100 bases:

>100_bases
AGCACAGGTTTTTTTAGAATAAAAAAGTCTGAATTTTGTGTTAAAATGATATTAGAAAAGGAGGCAGTGCAATGGAAGTT
TTACGAATTGGAAATATAAA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MRLRHKPYAMDRIKEYSQFVIGNPEEHRGNWKELFGNDHPIHIEVGTGRGRFVYEMAKANPDINYIGIEKFTSVIVDALD
KLIEKELPNLKLINKDAEDLTVFFTKGEIDRVYLNFSDPWPKKRHAKRRLTYKTFLRNYEEVLVKDGEIHFKTDNQALFE
YSLMSMAEYGMVFTFLSLDLHNSDFEGNIMTEYEEKFSSKGHRIYRVEAKYRTEPVQ

Sequences:

>Translated_217_residues
MRLRHKPYAMDRIKEYSQFVIGNPEEHRGNWKELFGNDHPIHIEVGTGRGRFVYEMAKANPDINYIGIEKFTSVIVDALD
KLIEKELPNLKLINKDAEDLTVFFTKGEIDRVYLNFSDPWPKKRHAKRRLTYKTFLRNYEEVLVKDGEIHFKTDNQALFE
YSLMSMAEYGMVFTFLSLDLHNSDFEGNIMTEYEEKFSSKGHRIYRVEAKYRTEPVQ
>Mature_217_residues
MRLRHKPYAMDRIKEYSQFVIGNPEEHRGNWKELFGNDHPIHIEVGTGRGRFVYEMAKANPDINYIGIEKFTSVIVDALD
KLIEKELPNLKLINKDAEDLTVFFTKGEIDRVYLNFSDPWPKKRHAKRRLTYKTFLRNYEEVLVKDGEIHFKTDNQALFE
YSLMSMAEYGMVFTFLSLDLHNSDFEGNIMTEYEEKFSSKGHRIYRVEAKYRTEPVQ

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=176, Percent_Identity=30.1136363636364, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_BACCN (A7GTW9)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001376572.1
- ProteinModelPortal:   A7GTW9
- SMR:   A7GTW9
- STRING:   A7GTW9
- EnsemblBacteria:   EBBACT00000037079
- GeneID:   5346616
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_3362
- eggNOG:   COG0220
- GeneTree:   EBGT00050000000917
- HOGENOM:   HBG304014
- OMA:   LRNKPWA
- ProtClustDB:   PRK00121
- BioCyc:   BCER315749:BCER98_3362-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 25731; Mature: 25731

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: BINDING 44-44 BINDING 69-69 BINDING 96-96 BINDING 118-118 BINDING 122-122 BINDING 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRHKPYAMDRIKEYSQFVIGNPEEHRGNWKELFGNDHPIHIEVGTGRGRFVYEMAKAN
CCCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHCCCCCCEEEEEECCCCCEEEEEECCC
PDINYIGIEKFTSVIVDALDKLIEKELPNLKLINKDAEDLTVFFTKGEIDRVYLNFSDPW
CCCEEECHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCEEEEEEECCCCC
PKKRHAKRRLTYKTFLRNYEEVLVKDGEIHFKTDNQALFEYSLMSMAEYGMVFTFLSLDL
CHHHHHHHHHHHHHHHHHHHHHHEECCEEEEECCCHHHHHHHHHHHHHHHHEEEHEEEEC
HNSDFEGNIMTEYEEKFSSKGHRIYRVEAKYRTEPVQ
CCCCCCCCEEHHHHHHHCCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure
MRLRHKPYAMDRIKEYSQFVIGNPEEHRGNWKELFGNDHPIHIEVGTGRGRFVYEMAKAN
CCCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHCCCCCCEEEEEECCCCCEEEEEECCC
PDINYIGIEKFTSVIVDALDKLIEKELPNLKLINKDAEDLTVFFTKGEIDRVYLNFSDPW
CCCEEECHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCEEEEEEECCCCC
PKKRHAKRRLTYKTFLRNYEEVLVKDGEIHFKTDNQALFEYSLMSMAEYGMVFTFLSLDL
CHHHHHHHHHHHHHHHHHHHHHHEECCEEEEECCCHHHHHHHHHHHHHHHHEEEHEEEEC
HNSDFEGNIMTEYEEKFSSKGHRIYRVEAKYRTEPVQ
CCCCCCCCEEHHHHHHHCCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA