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The map label for this gene is yitJ [H]

Identifier: 152976689

GI number: 152976689

Start: 3031440

End: 3033284

Strand: Reverse

Name: yitJ [H]

Synonym: Bcer98_2984

Alternate gene names: 152976689

Gene position: 3033284-3031440 (Counterclockwise)

Preceding gene: 152976693

Following gene: 152976688

Centisome position: 74.22

GC content: 38.75

Gene sequence:

>1845_bases
GTGAAATTACTAGATTTGTTATCAAAAGGGATTGTAATTGGTGATGGAGCAATTGGTACATTACTGCATTCACATGGACT
ACAAAGTAGTTTTGAAGAGTTAAATATATCAGATCCAGATTTAATTGTATCGATTCATAAACAATATGTAGCTGCTGGAG
CTGATGTGATTCAAACAAATACATATGGTGCAAATGAGGCGAAATTGCGTACATATGGCTTAGAAAATCAAGTTGTACAA
ATTAATAGAGCAGCAGTAAAACTCGCGAAAGCAGCTGCGAAGGAGCAAAATGCGATTTTAGGAACGATTGGTGGAATGAA
ACATATTGGTGCCGTTACGACAACGAATATAGAGCGAGAATTTATGCTACTTGAACAGGCTGGAGCGTTAATAGAAGAAG
AGGTAGATGGGCTACTGCTTGAAACATTTTATGATGAATTTGAGCTCATACATGCTATCAAAGTCCTAAGAAAACAAACA
GATATTCCGATTGTGGCACAATTAGCACTTCATGAGGCTGGTACGACCCAAAATGGAAATGATGTAAATGAGATATTAGC
ACAACTCATACATAATGGTGCAAATGTGGTCGGCTTAAATTGTCAATTAGGCCCACTTCCTATGACGGAAGCTTTAAAAA
TGATTTCGATTCCGAAAGACGGTTATTTATCTGCTTATCCAAATGCTGGACTTCCAAATTATATAGAAGGACGCTATATA
TACGAAGGAAGTCCGGCTTACTTCGAGACGATGACACCGAAGTTTATTGAACAGGGGGTTCGTTTATTAGGAGGTTGTTG
TGGTACCACACCGGCACATATTGAAAGAATGAAACGTGCAATTGCCAATCAAGTACCTGTTATAGAAAAGACGGTTGTTT
CAAGGCCGCCAATCCTTCATACGCATTCGGAGCGCTTTAGAACGCATAGTACTCTTGCAGAGAAAGCGAAAAAACAAACG
ACAGTTGTCGTTGAACTAGATCCACCAAAAACATTGGATACACAGCGTTTTTTTGAAGGAGCTAAAGCATTAAAGCGAGC
AGGTGCTGATGCGATTACATTGGCTGATAATTCGTTAGCTTCACCGCGCATTTCAAATATGGCGATGGGAGCGTTATTAA
CGAAGCATGATATACCAGTACTTACTCATTTAACGTGCCGAGATCACAATGTAATTGGATTACAATCACATCTACTTGGA
TTGTCAGTGCTAGGAATGGAAGAAGTACTCGCTTTAACAGGTGATCCAGCTCGGGTTGGGGATTTTCCAGGGGCTACTTC
TGTATATGATGTATCGTCTATTGAGCTTATTAAGATGATTCAGGAGATGAATGATGGTCGATCTATTTTAGGGAAATCGA
TTGGTCCGGCAACAAGGTTTTCTGTCGGAGGGGCATTTAATCCGCATGTAAGACATTTAAAGGCTGCAGTAAAGCGGATG
GAAAGAAAAATTGCAGCGGGTGCTGAATATTTCTTAACGCAGCCTATTTATGATAAAGCGTTGATTTATGAAGTATACAA
AGAAACGAAACATTTAGAACAACCTATTTTTATTGGAATTATGCCTTTAGTAAGCAAGCGGAATGCAGATTTTCTTCATT
TTGAAGTACCGGGGATTACGCTTCCAGATCAAGTGCGAGAAAGAATGAGAGAACATGAAACAGAAGCGGATGCGATTGAA
GAAGGGATCAGTATTGCACAGGAGTTAATTGATGAAGCATTGAAATATTTTCGTGGCATTTATCTTATTACGCCATTTTT
AAAATATGAGATTACAGAACATCTTGTGAAATATATAAAGGAAAAACAAGAAGTGAGAGAGGAAGTAACAAATGAGACGA
ATTGA

Upstream 100 bases:

>100_bases
CAAAACAATTGGTTTTGCGTGATAAGAGGTGGATCGATGAAGTCCCCCTCTTTAAGGTGAAGAGGGGGACTTTTTATATT
GATAGAAAAGAGGGAGATAA

Downstream 100 bases:

>100_bases
AGAAAGATTGCAGCAAGAAATCTTAATATTAGATGGTGCAATGGGAACGATGATTCAACAAGCCGATTTAACAGCTGATG
ATTTCGGTGGGGAAGAATAC

Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MKLLDLLSKGIVIGDGAIGTLLHSHGLQSSFEELNISDPDLIVSIHKQYVAAGADVIQTNTYGANEAKLRTYGLENQVVQ
INRAAVKLAKAAAKEQNAILGTIGGMKHIGAVTTTNIEREFMLLEQAGALIEEEVDGLLLETFYDEFELIHAIKVLRKQT
DIPIVAQLALHEAGTTQNGNDVNEILAQLIHNGANVVGLNCQLGPLPMTEALKMISIPKDGYLSAYPNAGLPNYIEGRYI
YEGSPAYFETMTPKFIEQGVRLLGGCCGTTPAHIERMKRAIANQVPVIEKTVVSRPPILHTHSERFRTHSTLAEKAKKQT
TVVVELDPPKTLDTQRFFEGAKALKRAGADAITLADNSLASPRISNMAMGALLTKHDIPVLTHLTCRDHNVIGLQSHLLG
LSVLGMEEVLALTGDPARVGDFPGATSVYDVSSIELIKMIQEMNDGRSILGKSIGPATRFSVGGAFNPHVRHLKAAVKRM
ERKIAAGAEYFLTQPIYDKALIYEVYKETKHLEQPIFIGIMPLVSKRNADFLHFEVPGITLPDQVRERMREHETEADAIE
EGISIAQELIDEALKYFRGIYLITPFLKYEITEHLVKYIKEKQEVREEVTNETN

Sequences:

>Translated_614_residues
MKLLDLLSKGIVIGDGAIGTLLHSHGLQSSFEELNISDPDLIVSIHKQYVAAGADVIQTNTYGANEAKLRTYGLENQVVQ
INRAAVKLAKAAAKEQNAILGTIGGMKHIGAVTTTNIEREFMLLEQAGALIEEEVDGLLLETFYDEFELIHAIKVLRKQT
DIPIVAQLALHEAGTTQNGNDVNEILAQLIHNGANVVGLNCQLGPLPMTEALKMISIPKDGYLSAYPNAGLPNYIEGRYI
YEGSPAYFETMTPKFIEQGVRLLGGCCGTTPAHIERMKRAIANQVPVIEKTVVSRPPILHTHSERFRTHSTLAEKAKKQT
TVVVELDPPKTLDTQRFFEGAKALKRAGADAITLADNSLASPRISNMAMGALLTKHDIPVLTHLTCRDHNVIGLQSHLLG
LSVLGMEEVLALTGDPARVGDFPGATSVYDVSSIELIKMIQEMNDGRSILGKSIGPATRFSVGGAFNPHVRHLKAAVKRM
ERKIAAGAEYFLTQPIYDKALIYEVYKETKHLEQPIFIGIMPLVSKRNADFLHFEVPGITLPDQVRERMREHETEADAIE
EGISIAQELIDEALKYFRGIYLITPFLKYEITEHLVKYIKEKQEVREEVTNETN
>Mature_614_residues
MKLLDLLSKGIVIGDGAIGTLLHSHGLQSSFEELNISDPDLIVSIHKQYVAAGADVIQTNTYGANEAKLRTYGLENQVVQ
INRAAVKLAKAAAKEQNAILGTIGGMKHIGAVTTTNIEREFMLLEQAGALIEEEVDGLLLETFYDEFELIHAIKVLRKQT
DIPIVAQLALHEAGTTQNGNDVNEILAQLIHNGANVVGLNCQLGPLPMTEALKMISIPKDGYLSAYPNAGLPNYIEGRYI
YEGSPAYFETMTPKFIEQGVRLLGGCCGTTPAHIERMKRAIANQVPVIEKTVVSRPPILHTHSERFRTHSTLAEKAKKQT
TVVVELDPPKTLDTQRFFEGAKALKRAGADAITLADNSLASPRISNMAMGALLTKHDIPVLTHLTCRDHNVIGLQSHLLG
LSVLGMEEVLALTGDPARVGDFPGATSVYDVSSIELIKMIQEMNDGRSILGKSIGPATRFSVGGAFNPHVRHLKAAVKRM
ERKIAAGAEYFLTQPIYDKALIYEVYKETKHLEQPIFIGIMPLVSKRNADFLHFEVPGITLPDQVRERMREHETEADAIE
EGISIAQELIDEALKYFRGIYLITPFLKYEITEHLVKYIKEKQEVREEVTNETN

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=343, Percent_Identity=28.5714285714286, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI13162290, Length=317, Percent_Identity=27.1293375394322, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI157266337, Length=317, Percent_Identity=27.4447949526814, Blast_Score=93, Evalue=8e-19,
Organism=Escherichia coli, GI1790450, Length=325, Percent_Identity=28.9230769230769, Blast_Score=117, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17535405, Length=329, Percent_Identity=28.5714285714286, Blast_Score=128, Evalue=9e-30,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 67691; Mature: 67691

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLDLLSKGIVIGDGAIGTLLHSHGLQSSFEELNISDPDLIVSIHKQYVAAGADVIQTN
CCHHHHHHCCEEEECCHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHCCCHHEECC
TYGANEAKLRTYGLENQVVQINRAAVKLAKAAAKEQNAILGTIGGMKHIGAVTTTNIERE
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHCCEEECCCHHH
FMLLEQAGALIEEEVDGLLLETFYDEFELIHAIKVLRKQTDIPIVAQLALHEAGTTQNGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCC
DVNEILAQLIHNGANVVGLNCQLGPLPMTEALKMISIPKDGYLSAYPNAGLPNYIEGRYI
CHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCEECCCCCCCCCCCCCEEE
YEGSPAYFETMTPKFIEQGVRLLGGCCGTTPAHIERMKRAIANQVPVIEKTVVSRPPILH
ECCCCCHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCC
THSERFRTHSTLAEKAKKQTTVVVELDPPKTLDTQRFFEGAKALKRAGADAITLADNSLA
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCC
SPRISNMAMGALLTKHDIPVLTHLTCRDHNVIGLQSHLLGLSVLGMEEVLALTGDPARVG
CCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEHHHHHHHHHHHCHHHHHHHCCCCCCCC
DFPGATSVYDVSSIELIKMIQEMNDGRSILGKSIGPATRFSVGGAFNPHVRHLKAAVKRM
CCCCCCCEEECHHHHHHHHHHHHCCCHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHH
ERKIAAGAEYFLTQPIYDKALIYEVYKETKHLEQPIFIGIMPLVSKRNADFLHFEVPGIT
HHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCEEEEECCCCC
LPDQVRERMREHETEADAIEEGISIAQELIDEALKYFRGIYLITPFLKYEITEHLVKYIK
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
EKQEVREEVTNETN
HHHHHHHHHCCCCC
>Mature Secondary Structure
MKLLDLLSKGIVIGDGAIGTLLHSHGLQSSFEELNISDPDLIVSIHKQYVAAGADVIQTN
CCHHHHHHCCEEEECCHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHCCCHHEECC
TYGANEAKLRTYGLENQVVQINRAAVKLAKAAAKEQNAILGTIGGMKHIGAVTTTNIERE
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHCCEEECCCHHH
FMLLEQAGALIEEEVDGLLLETFYDEFELIHAIKVLRKQTDIPIVAQLALHEAGTTQNGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCC
DVNEILAQLIHNGANVVGLNCQLGPLPMTEALKMISIPKDGYLSAYPNAGLPNYIEGRYI
CHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCEECCCCCCCCCCCCCEEE
YEGSPAYFETMTPKFIEQGVRLLGGCCGTTPAHIERMKRAIANQVPVIEKTVVSRPPILH
ECCCCCHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCC
THSERFRTHSTLAEKAKKQTTVVVELDPPKTLDTQRFFEGAKALKRAGADAITLADNSLA
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCC
SPRISNMAMGALLTKHDIPVLTHLTCRDHNVIGLQSHLLGLSVLGMEEVLALTGDPARVG
CCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEHHHHHHHHHHHCHHHHHHHCCCCCCCC
DFPGATSVYDVSSIELIKMIQEMNDGRSILGKSIGPATRFSVGGAFNPHVRHLKAAVKRM
CCCCCCCEEECHHHHHHHHHHHHCCCHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHH
ERKIAAGAEYFLTQPIYDKALIYEVYKETKHLEQPIFIGIMPLVSKRNADFLHFEVPGIT
HHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCEEEEECCCCC
LPDQVRERMREHETEADAIEEGISIAQELIDEALKYFRGIYLITPFLKYEITEHLVKYIK
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
EKQEVREEVTNETN
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]