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The map label for this gene is folD [H]

Identifier: 152976585

GI number: 152976585

Start: 2948735

End: 2949595

Strand: Reverse

Name: folD [H]

Synonym: Bcer98_2874

Alternate gene names: 152976585

Gene position: 2949595-2948735 (Counterclockwise)

Preceding gene: 152976586

Following gene: 152976584

Centisome position: 72.17

GC content: 35.89

Gene sequence:

>861_bases
ATGGTAGCAGTAATTATCAAAGGAAATGAAGTTGCGGAGAAAAAACGAGCACAATTAAAAGAAGAAGTTGTGAAGTTAAA
AGAGCAAGGAATTGTACCAGGATTAGCAGTTATTTTAGTTGGAGAAGACCCTGCCTCTCGTTCTTATATAAAGGGAAAAC
AAAAAGGCTGTGAACAAGTGGGGATTTATTCAGAGCTTATCGAATTTCCAGATACGATTACTGAAGAGCGTTTACTTGCT
GAAATCGATCGTTTAAATGAGGATGATCGTATCAATGGAATATTAGTACAATTACCTTTACCGAAACATATTGAAGAGAA
AGCTATCATTGAAAGAATTTCACCTAAGAAGGATGTAGATGGATTCCATCCCATTAATATTGGGCGTATGATGACAGGAC
AAGATACATTCCTTCCCTGTACGCCACATGGCATTTTAGAATTAGTAAAAGAAACGAATATTGATATTTCTGGAAAACAT
GTTGTTGTCATTGGAAGAAGTAACATCGTTGGAAAACCAGTAGGACAACTATTTTTAAATGAAAATGCGACTGTTACGTA
TTGTCATTCTAAAACGAAAAATATAAAAGAATTGTCAAAGTTAGCTGATATTTTAGTTGTAGCTGTAGGAAGACCAAAGA
TGGTAACGGCCGATTATATAAAAGAAGGAGCAGTTGTCATTGATGTTGGTGTGAATCGCTTAGAGACAGGAAAACTATGC
GGCGATGTTGACTTTGAAAATGTACTAGATATTGCAGGCTACATTACACCGGTGCCAAAAGGTGTTGGTCCGATGACAAT
TACAATGCTTCTTCATAATACAGTTGAATCTGCTAAACGTGCAGGTTTTATCTGTAAATAA

Upstream 100 bases:

>100_bases
TTGCGAAAACATTCGGGGATGAGGAATCTCGTCGTTTTATTAACGGCGTTTTATCTAATATAAAAGATACACTGTAATTT
CTTTAAGGGGGAATCTTAAA

Downstream 100 bases:

>100_bases
TTTGAAGCGGCTATGAGGAGAGAGAAATGGAAAAGCAATATTTAACCGTTACAGCATTAACACGTTATATCAAAACAAAA
ATAGAGTATGATCCGCATTT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MVAVIIKGNEVAEKKRAQLKEEVVKLKEQGIVPGLAVILVGEDPASRSYIKGKQKGCEQVGIYSELIEFPDTITEERLLA
EIDRLNEDDRINGILVQLPLPKHIEEKAIIERISPKKDVDGFHPINIGRMMTGQDTFLPCTPHGILELVKETNIDISGKH
VVVIGRSNIVGKPVGQLFLNENATVTYCHSKTKNIKELSKLADILVVAVGRPKMVTADYIKEGAVVIDVGVNRLETGKLC
GDVDFENVLDIAGYITPVPKGVGPMTITMLLHNTVESAKRAGFICK

Sequences:

>Translated_286_residues
MVAVIIKGNEVAEKKRAQLKEEVVKLKEQGIVPGLAVILVGEDPASRSYIKGKQKGCEQVGIYSELIEFPDTITEERLLA
EIDRLNEDDRINGILVQLPLPKHIEEKAIIERISPKKDVDGFHPINIGRMMTGQDTFLPCTPHGILELVKETNIDISGKH
VVVIGRSNIVGKPVGQLFLNENATVTYCHSKTKNIKELSKLADILVVAVGRPKMVTADYIKEGAVVIDVGVNRLETGKLC
GDVDFENVLDIAGYITPVPKGVGPMTITMLLHNTVESAKRAGFICK
>Mature_286_residues
MVAVIIKGNEVAEKKRAQLKEEVVKLKEQGIVPGLAVILVGEDPASRSYIKGKQKGCEQVGIYSELIEFPDTITEERLLA
EIDRLNEDDRINGILVQLPLPKHIEEKAIIERISPKKDVDGFHPINIGRMMTGQDTFLPCTPHGILELVKETNIDISGKH
VVVIGRSNIVGKPVGQLFLNENATVTYCHSKTKNIKELSKLADILVVAVGRPKMVTADYIKEGAVVIDVGVNRLETGKLC
GDVDFENVLDIAGYITPVPKGVGPMTITMLLHNTVESAKRAGFICK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=47.9310344827586, Blast_Score=255, Evalue=3e-68,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=49.3197278911565, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=47.2789115646259, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI36796743, Length=229, Percent_Identity=24.8908296943231, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=49.6402877697842, Blast_Score=275, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17568735, Length=292, Percent_Identity=47.6027397260274, Blast_Score=237, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=45.4545454545455, Blast_Score=252, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=42.6174496644295, Blast_Score=235, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6322933, Length=320, Percent_Identity=27.8125, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=47.0588235294118, Blast_Score=257, Evalue=6e-69,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=47.0588235294118, Blast_Score=257, Evalue=6e-69,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=47.0588235294118, Blast_Score=257, Evalue=8e-69,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=47.0588235294118, Blast_Score=257, Evalue=8e-69,
Organism=Drosophila melanogaster, GI17136816, Length=295, Percent_Identity=44.7457627118644, Blast_Score=240, Evalue=6e-64,
Organism=Drosophila melanogaster, GI17136818, Length=295, Percent_Identity=44.7457627118644, Blast_Score=240, Evalue=7e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31321; Mature: 31321

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAVIIKGNEVAEKKRAQLKEEVVKLKEQGIVPGLAVILVGEDPASRSYIKGKQKGCEQV
CEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCHHHH
GIYSELIEFPDTITEERLLAEIDRLNEDDRINGILVQLPLPKHIEEKAIIERISPKKDVD
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCC
GFHPINIGRMMTGQDTFLPCTPHGILELVKETNIDISGKHVVVIGRSNIVGKPVGQLFLN
CCCCCCCCEEECCCCCEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHEEEC
ENATVTYCHSKTKNIKELSKLADILVVAVGRPKMVTADYIKEGAVVIDVGVNRLETGKLC
CCCEEEEECCCCCCHHHHHHHHHHHEEECCCCCEEEHHHHHCCCEEEEECCCEECCCCCC
GDVDFENVLDIAGYITPVPKGVGPMTITMLLHNTVESAKRAGFICK
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVAVIIKGNEVAEKKRAQLKEEVVKLKEQGIVPGLAVILVGEDPASRSYIKGKQKGCEQV
CEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCHHHH
GIYSELIEFPDTITEERLLAEIDRLNEDDRINGILVQLPLPKHIEEKAIIERISPKKDVD
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCC
GFHPINIGRMMTGQDTFLPCTPHGILELVKETNIDISGKHVVVIGRSNIVGKPVGQLFLN
CCCCCCCCEEECCCCCEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHEEEC
ENATVTYCHSKTKNIKELSKLADILVVAVGRPKMVTADYIKEGAVVIDVGVNRLETGKLC
CCCEEEEECCCCCCHHHHHHHHHHHEEECCCCCEEEHHHHHCCCEEEEECCCEECCCCCC
GDVDFENVLDIAGYITPVPKGVGPMTITMLLHNTVESAKRAGFICK
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA