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The map label for this gene is yfkN [H]

Identifier: 152976532

GI number: 152976532

Start: 2896754

End: 2898991

Strand: Reverse

Name: yfkN [H]

Synonym: Bcer98_2821

Alternate gene names: 152976532

Gene position: 2898991-2896754 (Counterclockwise)

Preceding gene: 152976535

Following gene: 152976530

Centisome position: 70.93

GC content: 37.22

Gene sequence:

>2238_bases
GTGAAAAAATCAAAGAAGGTGTTAGCTGGAGCAACGCTTGCATTAGGTGTTATAGCCCCGCAAGTAGTACCAACCACTGC
TCATGCGGAAGGACAGACAGAGGAGAGCACAGTTCATTTACGAATTTTAGAAACATCTGATATTCATGTGAACTTAATGA
ATTATGATTATTATCAAACGAAAACAGATAATAAAGTAGGGCTTGTTCAAACGGCAACACTTGTCAATCAAGCGCGTGAA
GAAGTAAAAAACTCTGTTTTATTTGATGACGGAGATGCGATACAAGGAACACCGCTTGGGGACTATGTAGCGCAAAAAGG
GTTGGATGGGAATTATATACATCCATTATATCGTGTAATGAATTTAATGAAGTATGACGTCATTTCTTTAGGAAATCATG
AGTTTAACTATGGCTTAGATTATTTAAAGAAAGCAATTAGTAAGACTGAATTTCCAGTTGTGAATTCAAATGTGTATAAA
GATGACCATGATGATAATGAAGAAAATGATGAAAATTACTTTAAGCCATATCATATTTTTGAAAAAGAAGTAGAAGATGA
ATCCGGTCAAAAGCATAAGGTGAAGATTGGGGTAATGGGATTTGTACCACCACAAATTATGAACTGGGATAAGGCGAATT
TAGAAGGAAAAGTAAAAGCGAAAGATATCGTCCAAACAGCTAAGAAAATGGTTCCAAAAATGAAAGCAGAAGGTGCAGAT
ATTATCGTTGCGCTAGCGCACTCTGGTGTGGATAAAAGCGGTTACAATACAGGGATGGAAAACGCTTCATTTTATTTAAC
AGAAGTTCCTGGTGTTGATGCAGTGTTGATGGGACACTCACATACAGTAGTCACAGATACATTTAACGGTGTACCTGTTG
TAATGCCAGGTGTGTTCGGTAGCAACTTAGGCGTTATTGATATGCAGCTGAAAAAAGTAGATGGTAAGTGGCAAGTTCAA
AAGGATAAATCTGTTCCAACAGTTCGCGCTATTGCAGATAGTAAAGGAAACGCATTAGTTAAATCTGACCAAAGGTTAGT
TGACGAGATTAAGGAGGAACACGAAGCGACGATTCAATATGTTAATACACCTGTTGGGAAAACAACAGCGCCAATTAATA
GTTATTTTTCACTTGTGCAAGACGATCCATCGGTCCAACTTGTGACACAGGCGCAGAAATGGTATGTAGAAAAGTTGTTT
GCTGAAAATGGACAATATAGCAAGTATAAAGGCATTCCTATATTATCGGCAGGTGCACCATTTAAGGCAGGGGGAAGAAA
TGGTGCGACATATTATACAGATATTCCAGCGGGTACTCTAGCTATTAAAAACGTAGCCGATTTATATGTATATCCGAATA
CGTTATATGCTGTGAAAGTAAATGGTGCACAAGTAAAAGAATGGCTTGAAATGTCGGCAGGGCAGTTTAATCAAATTGAC
CCAGCTAAATCTGAAGAACAATCACTTGTTAATGTAAGCTATCCTACATATAATTTTGATATTTTAGATGGCCTGAAATA
TGAAATTGATGTTACACAACCAGCGAAGTACGATAAAGACGGAAAAGTGGTACATCCAACTGCAAATCGCATTATCAATA
TGACATATGACGGAAAACCGGTAACAGATGAACAAGAATTTATTGTTGCTACAAATAACTATCGTGGTAGCAGTCAAACA
TTCCCTGGTGTAAATAAAGGGGAAGTGGTGTATCAATCACAAGATGAAACGCGTCAAATTATTGTGAAATATATGCAAGA
GACGCCAGTTATTAATCCAGCGGCAGATCAAAACTGGTCATTTAAGCCAATCAATGCTGAAAAGTTAAATATTACATTTG
ATTCTTCGCCAAATGCACAAAAGTATATTACGAAAGATGGAAACATTTCTTATGTTGGTCCATCGGAAAATGAATTTGCA
AAATATGCAATTGACTTAACGAACAAGAAGGAAGAAGAGAAGCCGGTACCACCAGTAGCGGATGAAGGAAATAAAGAAGA
GAAGCCGGTACCACCAGTAGCAGATGAAGGAAATAATGGAGATCAGGCGACAGCAGACGCTATCAATAAAAATGAAGATG
TAACAACTACAAATGTAAGCGAAGATAAAGAAACAAATAAAATAGAACGAGATCTTCCAAAAACGGGAGCAAATGTTTTT
TCAACAATTGGGGCAGGACTTGCTTTTATTGGAGCAGGTATGTTCATGTTATTTAGAAGAAAGAAAGCAAATAGATAA

Upstream 100 bases:

>100_bases
GTCAGATAAGTGACGACATATCGCTCTATTAAGAAGAAAAAAGAGAGAAAGGTATTACAAGCTTATTGTATTTGGGACAA
TTTTATAAGGGGGATTTTTT

Downstream 100 bases:

>100_bases
GTAAAAGAGGGTTACCGACGTGGTAATCCTCTTTTTACTTAAGAAATTGTCACAGATTTTTCTTTCTTCCAAGAAAGACC
CAATCCTGTAAAGCCAACGA

Product: bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase precursor protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 745; Mature: 745

Protein sequence:

>745_residues
MKKSKKVLAGATLALGVIAPQVVPTTAHAEGQTEESTVHLRILETSDIHVNLMNYDYYQTKTDNKVGLVQTATLVNQARE
EVKNSVLFDDGDAIQGTPLGDYVAQKGLDGNYIHPLYRVMNLMKYDVISLGNHEFNYGLDYLKKAISKTEFPVVNSNVYK
DDHDDNEENDENYFKPYHIFEKEVEDESGQKHKVKIGVMGFVPPQIMNWDKANLEGKVKAKDIVQTAKKMVPKMKAEGAD
IIVALAHSGVDKSGYNTGMENASFYLTEVPGVDAVLMGHSHTVVTDTFNGVPVVMPGVFGSNLGVIDMQLKKVDGKWQVQ
KDKSVPTVRAIADSKGNALVKSDQRLVDEIKEEHEATIQYVNTPVGKTTAPINSYFSLVQDDPSVQLVTQAQKWYVEKLF
AENGQYSKYKGIPILSAGAPFKAGGRNGATYYTDIPAGTLAIKNVADLYVYPNTLYAVKVNGAQVKEWLEMSAGQFNQID
PAKSEEQSLVNVSYPTYNFDILDGLKYEIDVTQPAKYDKDGKVVHPTANRIINMTYDGKPVTDEQEFIVATNNYRGSSQT
FPGVNKGEVVYQSQDETRQIIVKYMQETPVINPAADQNWSFKPINAEKLNITFDSSPNAQKYITKDGNISYVGPSENEFA
KYAIDLTNKKEEEKPVPPVADEGNKEEKPVPPVADEGNNGDQATADAINKNEDVTTTNVSEDKETNKIERDLPKTGANVF
STIGAGLAFIGAGMFMLFRRKKANR

Sequences:

>Translated_745_residues
MKKSKKVLAGATLALGVIAPQVVPTTAHAEGQTEESTVHLRILETSDIHVNLMNYDYYQTKTDNKVGLVQTATLVNQARE
EVKNSVLFDDGDAIQGTPLGDYVAQKGLDGNYIHPLYRVMNLMKYDVISLGNHEFNYGLDYLKKAISKTEFPVVNSNVYK
DDHDDNEENDENYFKPYHIFEKEVEDESGQKHKVKIGVMGFVPPQIMNWDKANLEGKVKAKDIVQTAKKMVPKMKAEGAD
IIVALAHSGVDKSGYNTGMENASFYLTEVPGVDAVLMGHSHTVVTDTFNGVPVVMPGVFGSNLGVIDMQLKKVDGKWQVQ
KDKSVPTVRAIADSKGNALVKSDQRLVDEIKEEHEATIQYVNTPVGKTTAPINSYFSLVQDDPSVQLVTQAQKWYVEKLF
AENGQYSKYKGIPILSAGAPFKAGGRNGATYYTDIPAGTLAIKNVADLYVYPNTLYAVKVNGAQVKEWLEMSAGQFNQID
PAKSEEQSLVNVSYPTYNFDILDGLKYEIDVTQPAKYDKDGKVVHPTANRIINMTYDGKPVTDEQEFIVATNNYRGSSQT
FPGVNKGEVVYQSQDETRQIIVKYMQETPVINPAADQNWSFKPINAEKLNITFDSSPNAQKYITKDGNISYVGPSENEFA
KYAIDLTNKKEEEKPVPPVADEGNKEEKPVPPVADEGNNGDQATADAINKNEDVTTTNVSEDKETNKIERDLPKTGANVF
STIGAGLAFIGAGMFMLFRRKKANR
>Mature_745_residues
MKKSKKVLAGATLALGVIAPQVVPTTAHAEGQTEESTVHLRILETSDIHVNLMNYDYYQTKTDNKVGLVQTATLVNQARE
EVKNSVLFDDGDAIQGTPLGDYVAQKGLDGNYIHPLYRVMNLMKYDVISLGNHEFNYGLDYLKKAISKTEFPVVNSNVYK
DDHDDNEENDENYFKPYHIFEKEVEDESGQKHKVKIGVMGFVPPQIMNWDKANLEGKVKAKDIVQTAKKMVPKMKAEGAD
IIVALAHSGVDKSGYNTGMENASFYLTEVPGVDAVLMGHSHTVVTDTFNGVPVVMPGVFGSNLGVIDMQLKKVDGKWQVQ
KDKSVPTVRAIADSKGNALVKSDQRLVDEIKEEHEATIQYVNTPVGKTTAPINSYFSLVQDDPSVQLVTQAQKWYVEKLF
AENGQYSKYKGIPILSAGAPFKAGGRNGATYYTDIPAGTLAIKNVADLYVYPNTLYAVKVNGAQVKEWLEMSAGQFNQID
PAKSEEQSLVNVSYPTYNFDILDGLKYEIDVTQPAKYDKDGKVVHPTANRIINMTYDGKPVTDEQEFIVATNNYRGSSQT
FPGVNKGEVVYQSQDETRQIIVKYMQETPVINPAADQNWSFKPINAEKLNITFDSSPNAQKYITKDGNISYVGPSENEFA
KYAIDLTNKKEEEKPVPPVADEGNKEEKPVPPVADEGNNGDQATADAINKNEDVTTTNVSEDKETNKIERDLPKTGANVF
STIGAGLAFIGAGMFMLFRRKKANR

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=638, Percent_Identity=46.551724137931, Blast_Score=583, Evalue=1e-167,
Organism=Escherichia coli, GI1786687, Length=221, Percent_Identity=25.3393665158371, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922444, Length=388, Percent_Identity=24.7422680412371, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28573524, Length=219, Percent_Identity=28.7671232876712, Blast_Score=69, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922446, Length=454, Percent_Identity=23.7885462555066, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24654424, Length=454, Percent_Identity=23.7885462555066, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 82375; Mature: 82375

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSKKVLAGATLALGVIAPQVVPTTAHAEGQTEESTVHLRILETSDIHVNLMNYDYYQT
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCEEEEEEECEEEEC
KTDNKVGLVQTATLVNQAREEVKNSVLFDDGDAIQGTPLGDYVAQKGLDGNYIHPLYRVM
CCCCCEEEEHHHHHHHHHHHHHHHCEEECCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHH
NLMKYDVISLGNHEFNYGLDYLKKAISKTEFPVVNSNVYKDDHDDNEENDENYFKPYHIF
HHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHH
EKEVEDESGQKHKVKIGVMGFVPPQIMNWDKANLEGKVKAKDIVQTAKKMVPKMKAEGAD
HHHHCCCCCCEEEEEEEEEECCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
IIVALAHSGVDKSGYNTGMENASFYLTEVPGVDAVLMGHSHTVVTDTFNGVPVVMPGVFG
EEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCEEEEECCCCCCEEECCCCC
SNLGVIDMQLKKVDGKWQVQKDKSVPTVRAIADSKGNALVKSDQRLVDEIKEEHEATIQY
CCCCEEEEEEEECCCCEEEECCCCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHEEEE
VNTPVGKTTAPINSYFSLVQDDPSVQLVTQAQKWYVEKLFAENGQYSKYKGIPILSAGAP
EECCCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCCEECCCEEEECCCC
FKAGGRNGATYYTDIPAGTLAIKNVADLYVYPNTLYAVKVNGAQVKEWLEMSAGQFNQID
CCCCCCCCCEEEEECCCCCEECCCCEEEEEECCEEEEEEECHHHHHHHHHHCCCCCCCCC
PAKSEEQSLVNVSYPTYNFDILDGLKYEIDVTQPAKYDKDGKVVHPTANRIINMTYDGKP
CCCCCCCCEEEEECCCEEEEEECCCEEEEEECCCCCCCCCCCEECCCCCCEEEEEECCCC
VTDEQEFIVATNNYRGSSQTFPGVNKGEVVYQSQDETRQIIVKYMQETPVINPAADQNWS
CCCCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCC
FKPINAEKLNITFDSSPNAQKYITKDGNISYVGPSENEFAKYAIDLTNKKEEEKPVPPVA
CCCCCCEEEEEEECCCCCCCEEEECCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCC
DEGNKEEKPVPPVADEGNNGDQATADAINKNEDVTTTNVSEDKETNKIERDLPKTGANVF
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEECCCCCCHHHHHHHHHCCCCHHHHH
STIGAGLAFIGAGMFMLFRRKKANR
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKSKKVLAGATLALGVIAPQVVPTTAHAEGQTEESTVHLRILETSDIHVNLMNYDYYQT
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCEEEEEEECEEEEC
KTDNKVGLVQTATLVNQAREEVKNSVLFDDGDAIQGTPLGDYVAQKGLDGNYIHPLYRVM
CCCCCEEEEHHHHHHHHHHHHHHHCEEECCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHH
NLMKYDVISLGNHEFNYGLDYLKKAISKTEFPVVNSNVYKDDHDDNEENDENYFKPYHIF
HHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHH
EKEVEDESGQKHKVKIGVMGFVPPQIMNWDKANLEGKVKAKDIVQTAKKMVPKMKAEGAD
HHHHCCCCCCEEEEEEEEEECCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
IIVALAHSGVDKSGYNTGMENASFYLTEVPGVDAVLMGHSHTVVTDTFNGVPVVMPGVFG
EEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCEEEEECCCCCCEEECCCCC
SNLGVIDMQLKKVDGKWQVQKDKSVPTVRAIADSKGNALVKSDQRLVDEIKEEHEATIQY
CCCCEEEEEEEECCCCEEEECCCCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHEEEE
VNTPVGKTTAPINSYFSLVQDDPSVQLVTQAQKWYVEKLFAENGQYSKYKGIPILSAGAP
EECCCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCCEECCCEEEECCCC
FKAGGRNGATYYTDIPAGTLAIKNVADLYVYPNTLYAVKVNGAQVKEWLEMSAGQFNQID
CCCCCCCCCEEEEECCCCCEECCCCEEEEEECCEEEEEEECHHHHHHHHHHCCCCCCCCC
PAKSEEQSLVNVSYPTYNFDILDGLKYEIDVTQPAKYDKDGKVVHPTANRIINMTYDGKP
CCCCCCCCEEEEECCCEEEEEECCCEEEEEECCCCCCCCCCCEECCCCCCEEEEEECCCC
VTDEQEFIVATNNYRGSSQTFPGVNKGEVVYQSQDETRQIIVKYMQETPVINPAADQNWS
CCCCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCC
FKPINAEKLNITFDSSPNAQKYITKDGNISYVGPSENEFAKYAIDLTNKKEEEKPVPPVA
CCCCCCEEEEEEECCCCCCCEEEECCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCC
DEGNKEEKPVPPVADEGNNGDQATADAINKNEDVTTTNVSEDKETNKIERDLPKTGANVF
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEECCCCCCHHHHHHHHHCCCCHHHHH
STIGAGLAFIGAGMFMLFRRKKANR
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]