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The map label for this gene is dacF [H]

Identifier: 152976485

GI number: 152976485

Start: 2854805

End: 2855983

Strand: Reverse

Name: dacF [H]

Synonym: Bcer98_2774

Alternate gene names: 152976485

Gene position: 2855983-2854805 (Counterclockwise)

Preceding gene: 152976490

Following gene: 152976484

Centisome position: 69.88

GC content: 35.88

Gene sequence:

>1179_bases
ATGAAGCGAGTTTTTGGAATACTTGTTTGTTGTATGTTATTGCTTTCTCATACTTCAGTTAGTTTTGCAAAATCGGAGAA
AGCAATAGAAGAAACAAAGCCGAAGTTAGCAGAGCAAGCCTCGTCAGCGATCGTAATTGAGCAAGATACAGGTAAAGTTT
TATTTGAAAAGAACCCAAATGAAAAATTACCACCTGCTAGTATGACAAAGATTATGACAATGTTACTTATTATGGAACAA
GTAGAAAAAGGAAAATTAAAGCTAGAAGATAAAGTAAGAACGAGTGAGCATGCGGCTTCAATGGGAGGATCGCAAATTTT
TCTTGAACCTGGTGAAGAAATGACAGTAAATGAGATGCTAAAAGGTATTGCAATTGCATCAGGAAATGATGCTTCTGTTG
CAGTTGCTGAGCATATTGCTGGGTCAGAAGAAGGATTTGTCAACATGATGAATAAAAGAGCGAAGGAACTAGGTTTAAAA
AATACACATTTTCAAAATCCAACAGGCCTTCCTGCAAAGAATCATTATTCGACAGCAATTGATATGGCAATTATAGCGAA
AGAATTAATGAAATATCCGCTTATTCGTAAATATACCGGAAAGTATGAGGATTATTTACGTGAAAACACGGATAAAAAGT
TTTGGCTTGTTAATACAAATAAATTAGTTCGTTTTTACCCAGGAGTAGATGGAGTGAAAACTGGATTTACGACAGAAGCG
AAGTATTGTTTAACAGCATCGGCAGAAAAAAATGGCATGCGTGTTATTTCTGTTGTAATGGGAGCGCCTACATCGAAAGA
GAGAAATAGTCAAGTGACAAAGCTTCTTGATTATGCTTTTGGACAATATACAACGAAGAAGTTATATAAGCGCGGGGAAA
AGATTCAGACCGTACAAGTAGGAAAAGGAAAAAAAGAAAAAGTGGATTTAGTTGCAGCTGATAATGTTTCCCTTCTGATG
AAAAAGGGCGAAGATATGGACAAGATCAAGAAAGAAATTATTGCTGAAAAGAAAGTGAAAGCGCCGATTAAAAAAGGGGA
TGCACTTGGTACGCTTGTAATTACAAAAGATAGAGAAGTGTTATTAAAACAGACCATTGTGGCAAAAGAAGATGTCGACG
CAGCAAGTTGGTGGGAACTTTTTAAAAGAAGTTTTGGGATATTTTCAACATCAAAATAG

Upstream 100 bases:

>100_bases
TACAATAAAATAATTTGTCAACAGAAAATGATAGAAAAATATGGTATGAAACATATGTAAACTGGAAAAAATAGGATTGA
TATAGAATGGAGGGTTAGTC

Downstream 100 bases:

>100_bases
TAGTCAATCTTTTGTGTGATTCGATCAGAATAGAGGAAATACGTAGAATAATGGCACAAAATCTACACATTATGGCGAAA
TAGTACTTCTTTTGTCATGA

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKRVFGILVCCMLLLSHTSVSFAKSEKAIEETKPKLAEQASSAIVIEQDTGKVLFEKNPNEKLPPASMTKIMTMLLIMEQ
VEKGKLKLEDKVRTSEHAASMGGSQIFLEPGEEMTVNEMLKGIAIASGNDASVAVAEHIAGSEEGFVNMMNKRAKELGLK
NTHFQNPTGLPAKNHYSTAIDMAIIAKELMKYPLIRKYTGKYEDYLRENTDKKFWLVNTNKLVRFYPGVDGVKTGFTTEA
KYCLTASAEKNGMRVISVVMGAPTSKERNSQVTKLLDYAFGQYTTKKLYKRGEKIQTVQVGKGKKEKVDLVAADNVSLLM
KKGEDMDKIKKEIIAEKKVKAPIKKGDALGTLVITKDREVLLKQTIVAKEDVDAASWWELFKRSFGIFSTSK

Sequences:

>Translated_392_residues
MKRVFGILVCCMLLLSHTSVSFAKSEKAIEETKPKLAEQASSAIVIEQDTGKVLFEKNPNEKLPPASMTKIMTMLLIMEQ
VEKGKLKLEDKVRTSEHAASMGGSQIFLEPGEEMTVNEMLKGIAIASGNDASVAVAEHIAGSEEGFVNMMNKRAKELGLK
NTHFQNPTGLPAKNHYSTAIDMAIIAKELMKYPLIRKYTGKYEDYLRENTDKKFWLVNTNKLVRFYPGVDGVKTGFTTEA
KYCLTASAEKNGMRVISVVMGAPTSKERNSQVTKLLDYAFGQYTTKKLYKRGEKIQTVQVGKGKKEKVDLVAADNVSLLM
KKGEDMDKIKKEIIAEKKVKAPIKKGDALGTLVITKDREVLLKQTIVAKEDVDAASWWELFKRSFGIFSTSK
>Mature_392_residues
MKRVFGILVCCMLLLSHTSVSFAKSEKAIEETKPKLAEQASSAIVIEQDTGKVLFEKNPNEKLPPASMTKIMTMLLIMEQ
VEKGKLKLEDKVRTSEHAASMGGSQIFLEPGEEMTVNEMLKGIAIASGNDASVAVAEHIAGSEEGFVNMMNKRAKELGLK
NTHFQNPTGLPAKNHYSTAIDMAIIAKELMKYPLIRKYTGKYEDYLRENTDKKFWLVNTNKLVRFYPGVDGVKTGFTTEA
KYCLTASAEKNGMRVISVVMGAPTSKERNSQVTKLLDYAFGQYTTKKLYKRGEKIQTVQVGKGKKEKVDLVAADNVSLLM
KKGEDMDKIKKEIIAEKKVKAPIKKGDALGTLVITKDREVLLKQTIVAKEDVDAASWWELFKRSFGIFSTSK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=384, Percent_Identity=34.375, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1786851, Length=344, Percent_Identity=33.7209302325581, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI87082022, Length=372, Percent_Identity=29.5698924731183, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI87082054, Length=234, Percent_Identity=31.6239316239316, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43623; Mature: 43623

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVFGILVCCMLLLSHTSVSFAKSEKAIEETKPKLAEQASSAIVIEQDTGKVLFEKNPN
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCC
EKLPPASMTKIMTMLLIMEQVEKGKLKLEDKVRTSEHAASMGGSQIFLEPGEEMTVNEML
CCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHH
KGIAIASGNDASVAVAEHIAGSEEGFVNMMNKRAKELGLKNTHFQNPTGLPAKNHYSTAI
HHEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
DMAIIAKELMKYPLIRKYTGKYEDYLRENTDKKFWLVNTNKLVRFYPGVDGVKTGFTTEA
HHHHHHHHHHHCCHHHHHCCHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCC
KYCLTASAEKNGMRVISVVMGAPTSKERNSQVTKLLDYAFGQYTTKKLYKRGEKIQTVQV
CEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
GKGKKEKVDLVAADNVSLLMKKGEDMDKIKKEIIAEKKVKAPIKKGDALGTLVITKDREV
CCCCCCCEEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHH
LLKQTIVAKEDVDAASWWELFKRSFGIFSTSK
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKRVFGILVCCMLLLSHTSVSFAKSEKAIEETKPKLAEQASSAIVIEQDTGKVLFEKNPN
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCC
EKLPPASMTKIMTMLLIMEQVEKGKLKLEDKVRTSEHAASMGGSQIFLEPGEEMTVNEML
CCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHH
KGIAIASGNDASVAVAEHIAGSEEGFVNMMNKRAKELGLKNTHFQNPTGLPAKNHYSTAI
HHEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
DMAIIAKELMKYPLIRKYTGKYEDYLRENTDKKFWLVNTNKLVRFYPGVDGVKTGFTTEA
HHHHHHHHHHHCCHHHHHCCHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCC
KYCLTASAEKNGMRVISVVMGAPTSKERNSQVTKLLDYAFGQYTTKKLYKRGEKIQTVQV
CEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
GKGKKEKVDLVAADNVSLLMKKGEDMDKIKKEIIAEKKVKAPIKKGDALGTLVITKDREV
CCCCCCCEEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHH
LLKQTIVAKEDVDAASWWELFKRSFGIFSTSK
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]