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The map label for this gene is ykuM [H]

Identifier: 152976396

GI number: 152976396

Start: 2768566

End: 2769423

Strand: Reverse

Name: ykuM [H]

Synonym: Bcer98_2684

Alternate gene names: 152976396

Gene position: 2769423-2768566 (Counterclockwise)

Preceding gene: 152976397

Following gene: 152976395

Centisome position: 67.76

GC content: 34.15

Gene sequence:

>858_bases
TTGCAAATCGATGATTTTCAAATGATGGTTGTCTTAGCGCAAGAATCCAATATGAGAAGAGCGGCAGAGCGTTTATTTGT
TTCGCAGCCTGCACTAAGTCAACGATTACAATCTATGGAGAAACAATGGGGAATGAAATTTTTTATTCGCTCGCAAAAGG
GATTGACAATTACACCAGAAGGGGAAAAAGTAGCTAGTTATGCGAAGGAGATGTTAGAAAGAGAAGAAGCGATTAAGAGT
GAGTTAGCAATCTACCACACTGAGACATATGGAACATTGAAAATTGCTGTTGCTTCTGTAATTGGACAATATTGGTTACC
TCCTGTTTTGAAAAAATTTGTACAAAAATATCCTTTTGTGAAGATTTCGCTTTTTACAGGTTGGAGTAGCGAAGTGCAAA
AGCATTTTTATGAAGGAGACGTCCATGTTGCTATTTTAAGAGGAACGCAAGAATATAAAGGGCACAAACAACAATTATTT
GAGGATGAATTATACTTAGTTGATACAGAAATAAAAGATATTTCTATGCTGAAGGATACAAATAGACCTTTTATACAATT
TAAAAGTGATTCAACATACTATGGACAAATTCAAAATTGGTGGTACGGGCTATTTTCGAATCCGCCTAAACGTACAATCG
TTGTAGATCAGATTGAAACGTGTAAGCAACTTGTTTTAAATGGCATTGGCTATGCTTTGTTGCCTTCTACAGTATTAAAA
GAAGTAAAGACAAATACATATAAAACACCTGTACAATTAACAAGAGAAACTTGGCTATTAACAAGTGATTCTGCTAGACA
ATTAAAGCAAGTGCAAGCATTTTTAGAAATTATAGAAGAAATTCAAAAGGAAAAATAA

Upstream 100 bases:

>100_bases
TGACAATACGATAGGGATTTTATGTTAGAAAGGTCAGCTTGGATATTCTCCATTGACTTAATGTTTGTTTTATATGTAGG
ACAAGGAAAGAGGGAGAAAG

Downstream 100 bases:

>100_bases
GATTCTTATCTTGCTACTAGCTCTTTCGTTTGATAGAGTAACAATATAAATGGATTTAGGAGGCAACAAGCATGAAAATG
ATGGATGCAAATGAAATTAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MQIDDFQMMVVLAQESNMRRAAERLFVSQPALSQRLQSMEKQWGMKFFIRSQKGLTITPEGEKVASYAKEMLEREEAIKS
ELAIYHTETYGTLKIAVASVIGQYWLPPVLKKFVQKYPFVKISLFTGWSSEVQKHFYEGDVHVAILRGTQEYKGHKQQLF
EDELYLVDTEIKDISMLKDTNRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGYALLPSTVLK
EVKTNTYKTPVQLTRETWLLTSDSARQLKQVQAFLEIIEEIQKEK

Sequences:

>Translated_285_residues
MQIDDFQMMVVLAQESNMRRAAERLFVSQPALSQRLQSMEKQWGMKFFIRSQKGLTITPEGEKVASYAKEMLEREEAIKS
ELAIYHTETYGTLKIAVASVIGQYWLPPVLKKFVQKYPFVKISLFTGWSSEVQKHFYEGDVHVAILRGTQEYKGHKQQLF
EDELYLVDTEIKDISMLKDTNRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGYALLPSTVLK
EVKTNTYKTPVQLTRETWLLTSDSARQLKQVQAFLEIIEEIQKEK
>Mature_285_residues
MQIDDFQMMVVLAQESNMRRAAERLFVSQPALSQRLQSMEKQWGMKFFIRSQKGLTITPEGEKVASYAKEMLEREEAIKS
ELAIYHTETYGTLKIAVASVIGQYWLPPVLKKFVQKYPFVKISLFTGWSSEVQKHFYEGDVHVAILRGTQEYKGHKQQLF
EDELYLVDTEIKDISMLKDTNRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGYALLPSTVLK
EVKTNTYKTPVQLTRETWLLTSDSARQLKQVQAFLEIIEEIQKEK

Specific function: This Protein Is A Positive Regulator Of Gene Expression For The Cysteine Regulon, A System Of 10 Or More Loci Involved In The Biosynthesis Of L-Cysteine From Inorganic Sulfate. The Inducer For Cysb Is N-Acetylserine. Cysb Inhibits Its Own Transcription.

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=137, Percent_Identity=34.3065693430657, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787806, Length=292, Percent_Identity=20.5479452054795, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787601, Length=145, Percent_Identity=26.8965517241379, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786401, Length=248, Percent_Identity=27.0161290322581, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790399, Length=244, Percent_Identity=23.3606557377049, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790208, Length=249, Percent_Identity=24.0963855421687, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI157672245, Length=115, Percent_Identity=33.0434782608696, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788706, Length=127, Percent_Identity=31.496062992126, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787589, Length=140, Percent_Identity=27.8571428571429, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1789506, Length=251, Percent_Identity=21.5139442231076, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693105, Length=127, Percent_Identity=26.7716535433071, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788887, Length=246, Percent_Identity=25.2032520325203, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33188; Mature: 33188

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIDDFQMMVVLAQESNMRRAAERLFVSQPALSQRLQSMEKQWGMKFFIRSQKGLTITPE
CCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCEEECCC
GEKVASYAKEMLEREEAIKSELAIYHTETYGTLKIAVASVIGQYWLPPVLKKFVQKYPFV
CHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCE
KISLFTGWSSEVQKHFYEGDVHVAILRGTQEYKGHKQQLFEDELYLVDTEIKDISMLKDT
EEEEECCCHHHHHHHHHCCCEEEEEEECCHHHCCHHHHHHCCCEEEEECCHHHHHHHHCC
NRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGYALLPSTVLK
CCCEEEECCCCCEEECHHHHEEHHCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHH
EVKTNTYKTPVQLTRETWLLTSDSARQLKQVQAFLEIIEEIQKEK
HHHCCCCCCHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQIDDFQMMVVLAQESNMRRAAERLFVSQPALSQRLQSMEKQWGMKFFIRSQKGLTITPE
CCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCEEECCC
GEKVASYAKEMLEREEAIKSELAIYHTETYGTLKIAVASVIGQYWLPPVLKKFVQKYPFV
CHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCE
KISLFTGWSSEVQKHFYEGDVHVAILRGTQEYKGHKQQLFEDELYLVDTEIKDISMLKDT
EEEEECCCHHHHHHHHHCCCEEEEEEECCHHHCCHHHHHHCCCEEEEECCHHHHHHHHCC
NRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGYALLPSTVLK
CCCEEEECCCCCEEECHHHHEEHHCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHH
EVKTNTYKTPVQLTRETWLLTSDSARQLKQVQAFLEIIEEIQKEK
HHHCCCCCCHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]