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The map label for this gene is sigF [H]

Identifier: 152976319

GI number: 152976319

Start: 2703448

End: 2704176

Strand: Reverse

Name: sigF [H]

Synonym: Bcer98_2607

Alternate gene names: 152976319

Gene position: 2704176-2703448 (Counterclockwise)

Preceding gene: 152976320

Following gene: 152976318

Centisome position: 66.16

GC content: 36.49

Gene sequence:

>729_bases
ATGACTGAGTTAATTGATGTAAAGGCCCTGTCGGATGAAGAATTTATTCAAAAATACGAAAAGTTCGTTTTTCATTTCAT
TCATAAAAAATATGGTCACAGATTACAAGCGATAAAAGACGATACAGGTCTTGAATTTGAGGATTTGGTGCAATGTGGCA
TGATTGCGCTAATTAAAGCAAGAGAACAATTCAAGCCTGAAATGGGATTTAAATTCCTTACGTTTGCTGTACCGAAAATA
ATTGGTGAAATTGGTAATGAGATTTGGAAGACACAGAAGTTGAAAATCGAACGAACTGTATATTATGCGAAGGGGAAAAT
CTTGAAAAATAAGTTGTCGGAAGAATCGGTGGACCATATCAGCGAAACTTTGGATATTGATCCTAAACTTGTTGAAGCTG
CACAAGATTATCAACCAGGGACATTCTCTATAGATAAAGTTTTCTATCCGGATGACAACGGTGAAGGGGGAGAAATTACT
TTAGAAAAACAACTTGAAGATGAAAGTGTAGATGTAGAAAGTACAGTGGAAAACAAAGTGATTCTTCATTCTTTTTTCGA
TACATTGCAGCATAGCGAATTGGTTGTGTGGGACATGCATTCTAAAGGAACAAGACAAGAAAATATCGGAAAACGTATAG
GGAAATCACAGGTTCAAGTTAGTCGGATTTTAGGGCGAATTAATAAGAGAGCAACGGCTTTTGGACAGAAGCAAGGGATT
GCGAAGTAA

Upstream 100 bases:

>100_bases
AGAAGTCCTAATTATGTAAATAAATTGAAACAAAGATGGGAAAAGCAAGGGTTATGGGAAGGACCAACAAACATGTGGAA
AATCAGAAAGGGGAAGGGAA

Downstream 100 bases:

>100_bases
GACCAATTTTGAATTTTGTATAAAAACGAGGTGGTATCGTGAATAGGTATATTGAAATACTTTCGAAAATAGATAGGGTG
TATGCAGAAAAATATAAAGG

Product: FliA/WhiG family RNA polymerase sigma factor

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MTELIDVKALSDEEFIQKYEKFVFHFIHKKYGHRLQAIKDDTGLEFEDLVQCGMIALIKAREQFKPEMGFKFLTFAVPKI
IGEIGNEIWKTQKLKIERTVYYAKGKILKNKLSEESVDHISETLDIDPKLVEAAQDYQPGTFSIDKVFYPDDNGEGGEIT
LEKQLEDESVDVESTVENKVILHSFFDTLQHSELVVWDMHSKGTRQENIGKRIGKSQVQVSRILGRINKRATAFGQKQGI
AK

Sequences:

>Translated_242_residues
MTELIDVKALSDEEFIQKYEKFVFHFIHKKYGHRLQAIKDDTGLEFEDLVQCGMIALIKAREQFKPEMGFKFLTFAVPKI
IGEIGNEIWKTQKLKIERTVYYAKGKILKNKLSEESVDHISETLDIDPKLVEAAQDYQPGTFSIDKVFYPDDNGEGGEIT
LEKQLEDESVDVESTVENKVILHSFFDTLQHSELVVWDMHSKGTRQENIGKRIGKSQVQVSRILGRINKRATAFGQKQGI
AK
>Mature_241_residues
TELIDVKALSDEEFIQKYEKFVFHFIHKKYGHRLQAIKDDTGLEFEDLVQCGMIALIKAREQFKPEMGFKFLTFAVPKII
GEIGNEIWKTQKLKIERTVYYAKGKILKNKLSEESVDHISETLDIDPKLVEAAQDYQPGTFSIDKVFYPDDNGEGGEITL
EKQLEDESVDVESTVENKVILHSFFDTLQHSELVVWDMHSKGTRQENIGKRIGKSQVQVSRILGRINKRATAFGQKQGIA
K

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes. It is responsible for directing gene

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

None

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27755; Mature: 27624

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELIDVKALSDEEFIQKYEKFVFHFIHKKYGHRLQAIKDDTGLEFEDLVQCGMIALIKA
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHH
REQFKPEMGFKFLTFAVPKIIGEIGNEIWKTQKLKIERTVYYAKGKILKNKLSEESVDHI
HHHHCHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHEEHHHHHHHHHHHHHHHHHHH
SETLDIDPKLVEAAQDYQPGTFSIDKVFYPDDNGEGGEITLEKQLEDESVDVESTVENKV
HHHCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHH
ILHSFFDTLQHSELVVWDMHSKGTRQENIGKRIGKSQVQVSRILGRINKRATAFGQKQGI
HHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCC
AK
CC
>Mature Secondary Structure 
TELIDVKALSDEEFIQKYEKFVFHFIHKKYGHRLQAIKDDTGLEFEDLVQCGMIALIKA
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHH
REQFKPEMGFKFLTFAVPKIIGEIGNEIWKTQKLKIERTVYYAKGKILKNKLSEESVDHI
HHHHCHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHEEHHHHHHHHHHHHHHHHHHH
SETLDIDPKLVEAAQDYQPGTFSIDKVFYPDDNGEGGEITLEKQLEDESVDVESTVENKV
HHHCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHH
ILHSFFDTLQHSELVVWDMHSKGTRQENIGKRIGKSQVQVSRILGRINKRATAFGQKQGI
HHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCC
AK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1391049 [H]