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The map label for this gene is sigG [H]

Identifier: 152976264

GI number: 152976264

Start: 2651822

End: 2652601

Strand: Reverse

Name: sigG [H]

Synonym: Bcer98_2552

Alternate gene names: 152976264

Gene position: 2652601-2651822 (Counterclockwise)

Preceding gene: 152976265

Following gene: 152976263

Centisome position: 64.9

GC content: 35.77

Gene sequence:

>780_bases
TTGACAAGAAATAAAGTAGAAATTTGCGGTGTTGATACAGCTAAACTTCCAGTACTCAAAAATGATGAGATGCGTAAATT
ATTTCGAGAAATGCAAAGTGGAGAGACAAGTGCAAGAGAGAAATTAGTGAATGGAAATTTACGACTTGTATTAAGTGTCA
TTCAACGATTTAACAATAGAGGAGAATTTGTGGATGATTTATTTCAAGTTGGCTGCATTGGACTCATGAAATCCATTGAT
AATTTTGATTTAAGTCAAAATGTTAAATTTTCAACATATGCTGTGCCGATGATTATTGGGGAAATACGAAGATATTTGCG
CGATAATAATCCAATCCGCGTGTCTCGTTCATTACGGGATATTGCCTATAAAGCATTGCAAGTAAGAGAGAAATTAATTG
CTGAAAACTCCAAAGAGCCAACAGCAATGGATATTGCGCAAGTTCTTGATGTTACGCATGAGGAAATTGTATTCGCGCTT
GATGCAATTCAAGATCCAGTTTCATTATTTGAGCCAATTTATAACGATGGGGGAGACCCTATTTTTGTAATGGATCAGCT
GAGCGATGAAAAACAAAAGGACGAGCAGTGGGTTGAAGAACTAGCACTAAAAGAAGGAATGAAACGACTCAATGATCGAG
AAAAGATGATTATTCGAAAGCGTTTTTTTCAAGGAAAAACACAAATGGAAGTTGCAGAAGAAATTGGAATTTCCCAAGCA
CAAGTATCAAGGTTAGAGAAGTCAGCGATTAAACAAATGAATAAGACAATTCAAGGCTAA

Upstream 100 bases:

>100_bases
TGACATGCTTATGAGAAAGAGAAGAAAAACTGTATGCATAAAATTCCCTCCAAAGGAGATACTTTACACTGTACAGCAAC
TCCCGATAGGAGGGAACACT

Downstream 100 bases:

>100_bases
GGTTTCCCCGTTTATGGGGAGACCTTTTTTACATATAAATTTGTTTATCCGCTATTTTAAGGTAGTAAGAGTTGGAGAGA
AACAAGAAAGATAGATGGGA

Product: sporulation sigma factor SigG

Products: NA

Alternate protein names: Stage III sporulation protein G [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTRNKVEICGVDTAKLPVLKNDEMRKLFREMQSGETSAREKLVNGNLRLVLSVIQRFNNRGEFVDDLFQVGCIGLMKSID
NFDLSQNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVREKLIAENSKEPTAMDIAQVLDVTHEEIVFAL
DAIQDPVSLFEPIYNDGGDPIFVMDQLSDEKQKDEQWVEELALKEGMKRLNDREKMIIRKRFFQGKTQMEVAEEIGISQA
QVSRLEKSAIKQMNKTIQG

Sequences:

>Translated_259_residues
MTRNKVEICGVDTAKLPVLKNDEMRKLFREMQSGETSAREKLVNGNLRLVLSVIQRFNNRGEFVDDLFQVGCIGLMKSID
NFDLSQNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVREKLIAENSKEPTAMDIAQVLDVTHEEIVFAL
DAIQDPVSLFEPIYNDGGDPIFVMDQLSDEKQKDEQWVEELALKEGMKRLNDREKMIIRKRFFQGKTQMEVAEEIGISQA
QVSRLEKSAIKQMNKTIQG
>Mature_258_residues
TRNKVEICGVDTAKLPVLKNDEMRKLFREMQSGETSAREKLVNGNLRLVLSVIQRFNNRGEFVDDLFQVGCIGLMKSIDN
FDLSQNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVREKLIAENSKEPTAMDIAQVLDVTHEEIVFALD
AIQDPVSLFEPIYNDGGDPIFVMDQLSDEKQKDEQWVEELALKEGMKRLNDREKMIIRKRFFQGKTQMEVAEEIGISQAQ
VSRLEKSAIKQMNKTIQG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes in the forespore [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=247, Percent_Identity=28.7449392712551, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789098, Length=260, Percent_Identity=26.5384615384615, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789871, Length=260, Percent_Identity=27.3076923076923, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014212
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29776; Mature: 29645

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRNKVEICGVDTAKLPVLKNDEMRKLFREMQSGETSAREKLVNGNLRLVLSVIQRFNNR
CCCCEEEECCCHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCC
GEFVDDLFQVGCIGLMKSIDNFDLSQNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRD
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHH
IAYKALQVREKLIAENSKEPTAMDIAQVLDVTHEEIVFALDAIQDPVSLFEPIYNDGGDP
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCE
IFVMDQLSDEKQKDEQWVEELALKEGMKRLNDREKMIIRKRFFQGKTQMEVAEEIGISQA
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHH
QVSRLEKSAIKQMNKTIQG
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2497052; 2459711; 9384377 [H]