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The map label for this gene is priA [H]

Identifier: 152976234

GI number: 152976234

Start: 2618860

End: 2621265

Strand: Reverse

Name: priA [H]

Synonym: Bcer98_2520

Alternate gene names: 152976234

Gene position: 2621265-2618860 (Counterclockwise)

Preceding gene: 152976235

Following gene: 152976233

Centisome position: 64.14

GC content: 37.66

Gene sequence:

>2406_bases
ATGAAATTTGCGAGTGTAATTGTTGATGTGCCGGCCCGTCAAACAGATAGGCCGTTTGACTATATCATTCCTAACAAGTG
GGAAGATATTGTGCAAACTGGTATGCGAGTCGTTGTTCCGTTTGGACCGCGAAAGTTACAAGGATTTATTATCGACATAA
AGGACTCTGTAGATGTAGAAATTAAAAAATTAAAATCATTACATGAAATATTAGACGTCACACCAGTTCTAAATGAAGAG
CTACTAAAGCTTGGCTTTTGGCTTACGGAAGAGACGTTATGCTATACAATTTCTGCTTTTCAAGTGATGCTTCCAACTGC
AATTAAAGCTACTTACAAGAAATTGTTACAGCTTCATAATCCGAATGAAGTTTCCGCCGAAATAGATCACTTATTTCAAG
GGAAAGATAGAATAGATTGGGAAGAAGTTGCTTCGCAGCCACAGCTATATCGCATGGTGCAAAAAGAAATTACAAACGGT
ACAGTTGAAGTAATTTATAAAGTAAAGGATAAAGTGCAAAAGAAAAAACAAAGAGTTGTTCAGCCTGAATTACAAGAGGA
AGGACTAGAAGCGGCGGCGTTTGAATTGAAAAGTAAAAAACAACAAGATATACTATATTATTTTATGGAAAATTACCACA
CTGTACCATTAAAAGTATTGAAAGAAGAATTGCAAATAACAGATGCTCCCATTAAATCGCTCGTAAAAAAAGGTTTATTG
TCTGAAAAATATATAGAGGTATATCGTAATCCTTATGATGAAGATGATTTTGAGAAGACCAAACCATTTCCATTAACAGA
TGAACAAAAGCAAGTGATTACACCCATTTTGTCAGCTATTACAAATGAAACTTACAAATCATTTTTACTATATGGAGTCA
CAGGAAGCGGAAAAACAGAAGTGTATTTACAGTCAATCGCTGCGGTATTAGAAAAAGGAAAAGAAGCGATTGTACTTGTT
CCGGAAATTGCCCTAACACCGCAAATGGTAGAGCGTTTCAAAGGGCGATTCGGTTCGCAAGTTGCGGTTCTTCATAGCGC
ACTTTCTGTTGGAGAAAAATATGATGAGTGGCGGAAAATTTTAAGAAAAGAAGTAAAAGTTGTAGTTGGTGCACGTTCTG
CGATTTTTGCTCCATTTGAAAACTTAGGGATTATTATTATTGATGAAGAGCATGAAGCAAGTTATAAGCAAGAGGATAAT
CCGAGATATCATGCTAGAGATGTTGCAATTTGGAGAGGACGATATCATTGTTGTCCAATCGTGCTTGGTAGTGCCACACC
GACACTTGAATCGTTTGCTAGGGCGAAGAAAGGTGTTTACGAGCTGTTAACGATGGAGAAGCGAATGAATGAGCAAGCAT
TGCCTACGGTAAATATTATAGATATGCGTGAAGAACTTCGCGATGGCAATCGTTCTATGTTCTCTAGAACATTACATGAA
AAAATTGCTGATCGGTTAAAGAAAAAGGAACAAATGGTGCTTTTCTTAAATCGCCGCGGACATTCTACATTTGTGATGTG
CCGAGATTGTGGTTATGTAGTACAATGTCCTCACTGTGATATATCACTTACGTATCATAAAATGAATTATCGTTTAAAGT
GCCACTATTGTAGTTATGAGGAACAGATGCCAACAGCTTGTCCGGCATGTAATAGTTCGCATATTCGTTTTTTTGGTACA
GGAACACAAAAGGTGGAAGAAGAATTAACAAAATTATTCCCAGAGGCACGAGTGATTCGAATGGATGTGGATACAACAAG
TCGTAAGGGAATGCATGAAAAACTATTAAAAGCATTTGGAGAAGAAAAAGCCGATATCTTACTTGGTACACAAATGATCG
CAAAAGGATTAGATTTTCCGAAAGTGACGCTTGTTGGTGTTCTAACAGCTGATACAATGCTTCATTTGCCGGATTTTAGG
GCGAGCGAAAAAACGTATCAACTATTAACACAAGTTAGCGGCAGAGCCGGACGTCACGACTTGCCAGGAGAAGTGGTGAT
TCAAACATATACACCGGAACATTATAGTATAGAGCTGGCAAAAGGCCAAAACTATGATTTATTTTTTGAACAGGAAATGC
AAATGAGAAGAATGAGGCAATATCCACCATACTATTATGTCGCACTTGTCACTGTATCGCATCCGGAGTTATTAAAGGCA
GTGCAGGTGACAGAAAAAATTGTCGGCTATTTACGGGCAAACTGTTCGCGGCAAACGATGGTCTTAGGTCCAGTTGCGTC
GCCGATTCCAAGGATAAAAGATAGATATCGTTATCAATGCATGATAAAATACAAACGGGAACCAAACTTAAAGTACGTAC
TCAAAATGGTAAATGAACATTATCAAGCAGAAATGCAAAAGGACTTACAAATCTCAATTGATTTTAATCCAACGGTGTTA
ATGTAA

Upstream 100 bases:

>100_bases
GAAAAGATGGGAATGTGATTGAGTTGCCACTTTTATCGAAAAAGGAAGTCGCTTATGAAATATTGAAGCAAATTGAATCG
ATGTTAGAAGATGATTGTAT

Downstream 100 bases:

>100_bases
CGGAGGGAATTATGGCAGTTTTAGAAATTGTAAAGCATCCAAATGAGGTGCTAGAGACCCCATGTGAACGGGTAATAAAC
TTTGATAAAAAGTTAGTGAT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MKFASVIVDVPARQTDRPFDYIIPNKWEDIVQTGMRVVVPFGPRKLQGFIIDIKDSVDVEIKKLKSLHEILDVTPVLNEE
LLKLGFWLTEETLCYTISAFQVMLPTAIKATYKKLLQLHNPNEVSAEIDHLFQGKDRIDWEEVASQPQLYRMVQKEITNG
TVEVIYKVKDKVQKKKQRVVQPELQEEGLEAAAFELKSKKQQDILYYFMENYHTVPLKVLKEELQITDAPIKSLVKKGLL
SEKYIEVYRNPYDEDDFEKTKPFPLTDEQKQVITPILSAITNETYKSFLLYGVTGSGKTEVYLQSIAAVLEKGKEAIVLV
PEIALTPQMVERFKGRFGSQVAVLHSALSVGEKYDEWRKILRKEVKVVVGARSAIFAPFENLGIIIIDEEHEASYKQEDN
PRYHARDVAIWRGRYHCCPIVLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVNIIDMREELRDGNRSMFSRTLHE
KIADRLKKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNYRLKCHYCSYEEQMPTACPACNSSHIRFFGT
GTQKVEEELTKLFPEARVIRMDVDTTSRKGMHEKLLKAFGEEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFR
ASEKTYQLLTQVSGRAGRHDLPGEVVIQTYTPEHYSIELAKGQNYDLFFEQEMQMRRMRQYPPYYYVALVTVSHPELLKA
VQVTEKIVGYLRANCSRQTMVLGPVASPIPRIKDRYRYQCMIKYKREPNLKYVLKMVNEHYQAEMQKDLQISIDFNPTVL
M

Sequences:

>Translated_801_residues
MKFASVIVDVPARQTDRPFDYIIPNKWEDIVQTGMRVVVPFGPRKLQGFIIDIKDSVDVEIKKLKSLHEILDVTPVLNEE
LLKLGFWLTEETLCYTISAFQVMLPTAIKATYKKLLQLHNPNEVSAEIDHLFQGKDRIDWEEVASQPQLYRMVQKEITNG
TVEVIYKVKDKVQKKKQRVVQPELQEEGLEAAAFELKSKKQQDILYYFMENYHTVPLKVLKEELQITDAPIKSLVKKGLL
SEKYIEVYRNPYDEDDFEKTKPFPLTDEQKQVITPILSAITNETYKSFLLYGVTGSGKTEVYLQSIAAVLEKGKEAIVLV
PEIALTPQMVERFKGRFGSQVAVLHSALSVGEKYDEWRKILRKEVKVVVGARSAIFAPFENLGIIIIDEEHEASYKQEDN
PRYHARDVAIWRGRYHCCPIVLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVNIIDMREELRDGNRSMFSRTLHE
KIADRLKKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNYRLKCHYCSYEEQMPTACPACNSSHIRFFGT
GTQKVEEELTKLFPEARVIRMDVDTTSRKGMHEKLLKAFGEEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFR
ASEKTYQLLTQVSGRAGRHDLPGEVVIQTYTPEHYSIELAKGQNYDLFFEQEMQMRRMRQYPPYYYVALVTVSHPELLKA
VQVTEKIVGYLRANCSRQTMVLGPVASPIPRIKDRYRYQCMIKYKREPNLKYVLKMVNEHYQAEMQKDLQISIDFNPTVL
M
>Mature_801_residues
MKFASVIVDVPARQTDRPFDYIIPNKWEDIVQTGMRVVVPFGPRKLQGFIIDIKDSVDVEIKKLKSLHEILDVTPVLNEE
LLKLGFWLTEETLCYTISAFQVMLPTAIKATYKKLLQLHNPNEVSAEIDHLFQGKDRIDWEEVASQPQLYRMVQKEITNG
TVEVIYKVKDKVQKKKQRVVQPELQEEGLEAAAFELKSKKQQDILYYFMENYHTVPLKVLKEELQITDAPIKSLVKKGLL
SEKYIEVYRNPYDEDDFEKTKPFPLTDEQKQVITPILSAITNETYKSFLLYGVTGSGKTEVYLQSIAAVLEKGKEAIVLV
PEIALTPQMVERFKGRFGSQVAVLHSALSVGEKYDEWRKILRKEVKVVVGARSAIFAPFENLGIIIIDEEHEASYKQEDN
PRYHARDVAIWRGRYHCCPIVLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVNIIDMREELRDGNRSMFSRTLHE
KIADRLKKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNYRLKCHYCSYEEQMPTACPACNSSHIRFFGT
GTQKVEEELTKLFPEARVIRMDVDTTSRKGMHEKLLKAFGEEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFR
ASEKTYQLLTQVSGRAGRHDLPGEVVIQTYTPEHYSIELAKGQNYDLFFEQEMQMRRMRQYPPYYYVALVTVSHPELLKA
VQVTEKIVGYLRANCSRQTMVLGPVASPIPRIKDRYRYQCMIKYKREPNLKYVLKMVNEHYQAEMQKDLQISIDFNPTVL
M

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=589, Percent_Identity=38.5398981324278, Blast_Score=421, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 92545; Mature: 92545

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFASVIVDVPARQTDRPFDYIIPNKWEDIVQTGMRVVVPFGPRKLQGFIIDIKDSVDVE
CCHHHEEEECCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCEEEEEEEEECCCCCHH
IKKLKSLHEILDVTPVLNEELLKLGFWLTEETLCYTISAFQVMLPTAIKATYKKLLQLHN
HHHHHHHHHHHHCCHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PNEVSAEIDHLFQGKDRIDWEEVASQPQLYRMVQKEITNGTVEVIYKVKDKVQKKKQRVV
CCHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
QPELQEEGLEAAAFELKSKKQQDILYYFMENYHTVPLKVLKEELQITDAPIKSLVKKGLL
CCCHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHCCC
SEKYIEVYRNPYDEDDFEKTKPFPLTDEQKQVITPILSAITNETYKSFLLYGVTGSGKTE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHH
VYLQSIAAVLEKGKEAIVLVPEIALTPQMVERFKGRFGSQVAVLHSALSVGEKYDEWRKI
HHHHHHHHHHHCCCCEEEEECCHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LRKEVKVVVGARSAIFAPFENLGIIIIDEEHEASYKQEDNPRYHARDVAIWRGRYHCCPI
HHHHHHHHHCCCHHHCCCHHHCCEEEEECCCCCCCCCCCCCCEECCEEEEECCCCEEEEE
VLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVNIIDMREELRDGNRSMFSRTLHE
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCEEEHHHHHHHHCCCCHHHHHHHHHH
KIADRLKKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNYRLKCHYCSYE
HHHHHHHHHCCEEEEEECCCCCEEEEEECCCCEEECCCCCEEEEEEEECEEEEEEEECCH
EQMPTACPACNSSHIRFFGTGTQKVEEELTKLFPEARVIRMDVDTTSRKGMHEKLLKAFG
HCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHC
EEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFRASEKTYQLLTQVSGRAGRHD
CCCHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCC
LPGEVVIQTYTPEHYSIELAKGQNYDLFFEQEMQMRRMRQYPPYYYVALVTVSHPELLKA
CCCCEEEEEECCCCEEEEEECCCCCCEEEHHHHHHHHHHHCCCEEEEEEEEECCHHHHHH
VQVTEKIVGYLRANCSRQTMVLGPVASPIPRIKDRYRYQCMIKYKREPNLKYVLKMVNEH
HHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHEEEEEEEECCCCCHHHHHHHHHHH
YQAEMQKDLQISIDFNPTVLM
HHHHHCCCEEEEEECCCCEEC
>Mature Secondary Structure
MKFASVIVDVPARQTDRPFDYIIPNKWEDIVQTGMRVVVPFGPRKLQGFIIDIKDSVDVE
CCHHHEEEECCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCEEEEEEEEECCCCCHH
IKKLKSLHEILDVTPVLNEELLKLGFWLTEETLCYTISAFQVMLPTAIKATYKKLLQLHN
HHHHHHHHHHHHCCHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PNEVSAEIDHLFQGKDRIDWEEVASQPQLYRMVQKEITNGTVEVIYKVKDKVQKKKQRVV
CCHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
QPELQEEGLEAAAFELKSKKQQDILYYFMENYHTVPLKVLKEELQITDAPIKSLVKKGLL
CCCHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHCCC
SEKYIEVYRNPYDEDDFEKTKPFPLTDEQKQVITPILSAITNETYKSFLLYGVTGSGKTE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHH
VYLQSIAAVLEKGKEAIVLVPEIALTPQMVERFKGRFGSQVAVLHSALSVGEKYDEWRKI
HHHHHHHHHHHCCCCEEEEECCHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LRKEVKVVVGARSAIFAPFENLGIIIIDEEHEASYKQEDNPRYHARDVAIWRGRYHCCPI
HHHHHHHHHCCCHHHCCCHHHCCEEEEECCCCCCCCCCCCCCEECCEEEEECCCCEEEEE
VLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVNIIDMREELRDGNRSMFSRTLHE
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCEEEHHHHHHHHCCCCHHHHHHHHHH
KIADRLKKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNYRLKCHYCSYE
HHHHHHHHHCCEEEEEECCCCCEEEEEECCCCEEECCCCCEEEEEEEECEEEEEEEECCH
EQMPTACPACNSSHIRFFGTGTQKVEEELTKLFPEARVIRMDVDTTSRKGMHEKLLKAFG
HCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHC
EEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFRASEKTYQLLTQVSGRAGRHD
CCCHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCC
LPGEVVIQTYTPEHYSIELAKGQNYDLFFEQEMQMRRMRQYPPYYYVALVTVSHPELLKA
CCCCEEEEEECCCCEEEEEECCCCCCEEEHHHHHHHHHHHCCCEEEEEEEEECCHHHHHH
VQVTEKIVGYLRANCSRQTMVLGPVASPIPRIKDRYRYQCMIKYKREPNLKYVLKMVNEH
HHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHEEEEEEEECCCCCHHHHHHHHHHH
YQAEMQKDLQISIDFNPTVLM
HHHHHCCCEEEEEECCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]