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The map label for this gene is thiN [H]

Identifier: 152976225

GI number: 152976225

Start: 2609712

End: 2610353

Strand: Reverse

Name: thiN [H]

Synonym: Bcer98_2511

Alternate gene names: 152976225

Gene position: 2610353-2609712 (Counterclockwise)

Preceding gene: 152976226

Following gene: 152976223

Centisome position: 63.87

GC content: 35.67

Gene sequence:

>642_bases
ATGATTATTCATATTTTAGCAGGAGGACCTGAAGAACATTGTGCGGATTTTTCTTTATATGAAAATGACGAGGTAGTGTG
GGCAGCAGTTGACCGTGGGGTGTATCGCTTATTAAAACGAGGAATTGTTCCGGCTGTTGCGTTTGGTGATTATGATTCGG
TGACCGATGATGAGTTGGCTTGGATGCAAAAACAGACAGATGATTTACATATTGTTCCGAAGGAAAAAGATCAAACGGAT
TTAGAAATTGCTATTCGGTGGGCTTTAAATCAAAATCCGAAGTTCATTCGCATTTTTGGTGCTACTGGCGGGAGACTTGA
TCATGGTTTAGCTAATATACAAATGCTTTTGAAAGGTTTAGAAAAAGAAATAGATATGTATATTGTGGATCGAAAAAATG
AAATATGTGTAAAGAGTGTAGGAGCATATGATATTGAAGAAAATAAGCAGTTTCCATATGTTTCTTTTGTACCAGTTACA
AAGATTGTAAAAGGGATTACACTTCGCGGTTTTAAATATCCTTTAACAAATAGAACCATTGAATGGGGATCGACGCTTTG
CATTAGTAATGAACTTGCCAATGAAAAAGGTACTTTTTCATTTACTTCTGGCATATTAATGATGATAAGAAGCACTGATT
AA

Upstream 100 bases:

>100_bases
GCGTATTAGAAATGTATAAAGACCTGTTAGAACGGGATCACACAAAAAAGCAATCTACCGACTGGCAGATTGCTTTTTTT
TGATAGAAGGGGACGAAAAA

Downstream 100 bases:

>100_bases
AGAAACAAATTTGCTCCTAGCATCATATAGTAAACAAGTGGAGTAATGGGGATTAGGAGGGAAACCATGAGATTTTATAC
AATTAAGTTACCTAAATTTC

Product: thiamine pyrophosphokinase

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MIIHILAGGPEEHCADFSLYENDEVVWAAVDRGVYRLLKRGIVPAVAFGDYDSVTDDELAWMQKQTDDLHIVPKEKDQTD
LEIAIRWALNQNPKFIRIFGATGGRLDHGLANIQMLLKGLEKEIDMYIVDRKNEICVKSVGAYDIEENKQFPYVSFVPVT
KIVKGITLRGFKYPLTNRTIEWGSTLCISNELANEKGTFSFTSGILMMIRSTD

Sequences:

>Translated_213_residues
MIIHILAGGPEEHCADFSLYENDEVVWAAVDRGVYRLLKRGIVPAVAFGDYDSVTDDELAWMQKQTDDLHIVPKEKDQTD
LEIAIRWALNQNPKFIRIFGATGGRLDHGLANIQMLLKGLEKEIDMYIVDRKNEICVKSVGAYDIEENKQFPYVSFVPVT
KIVKGITLRGFKYPLTNRTIEWGSTLCISNELANEKGTFSFTSGILMMIRSTD
>Mature_213_residues
MIIHILAGGPEEHCADFSLYENDEVVWAAVDRGVYRLLKRGIVPAVAFGDYDSVTDDELAWMQKQTDDLHIVPKEKDQTD
LEIAIRWALNQNPKFIRIFGATGGRLDHGLANIQMLLKGLEKEIDMYIVDRKNEICVKSVGAYDIEENKQFPYVSFVPVT
KIVKGITLRGFKYPLTNRTIEWGSTLCISNELANEKGTFSFTSGILMMIRSTD

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI71997787, Length=227, Percent_Identity=22.0264317180617, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 24106; Mature: 24106

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIHILAGGPEEHCADFSLYENDEVVWAAVDRGVYRLLKRGIVPAVAFGDYDSVTDDELA
CEEEEEECCCHHHCCCCEEECCCCEEEEEHHHHHHHHHHCCCCCEEEECCCCCCCCHHHH
WMQKQTDDLHIVPKEKDQTDLEIAIRWALNQNPKFIRIFGATGGRLDHGLANIQMLLKGL
HHHCCCCCEEEEECCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
EKEIDMYIVDRKNEICVKSVGAYDIEENKQFPYVSFVPVTKIVKGITLRGFKYPLTNRTI
HHCCEEEEEECCCCEEEEECCCEECCCCCCCCEEEECCHHHHHCCCEECCEECCCCCCEE
EWGSTLCISNELANEKGTFSFTSGILMMIRSTD
CCCCEEEEEHHHCCCCCCEEECCEEEEEEEECC
>Mature Secondary Structure
MIIHILAGGPEEHCADFSLYENDEVVWAAVDRGVYRLLKRGIVPAVAFGDYDSVTDDELA
CEEEEEECCCHHHCCCCEEECCCCEEEEEHHHHHHHHHHCCCCCEEEECCCCCCCCHHHH
WMQKQTDDLHIVPKEKDQTDLEIAIRWALNQNPKFIRIFGATGGRLDHGLANIQMLLKGL
HHHCCCCCEEEEECCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
EKEIDMYIVDRKNEICVKSVGAYDIEENKQFPYVSFVPVTKIVKGITLRGFKYPLTNRTI
HHCCEEEEEECCCCEEEEECCCEECCCCCCCCEEEECCHHHHHCCCEECCEECCCCCCEE
EWGSTLCISNELANEKGTFSFTSGILMMIRSTD
CCCCEEEEEHHHCCCCCCEEECCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]