Click here to switch to the map view.

The map label for this gene is infB

Identifier: 152976179

GI number: 152976179

Start: 2559530

End: 2561599

Strand: Reverse

Name: infB

Synonym: Bcer98_2465

Alternate gene names: 152976179

Gene position: 2561599-2559530 (Counterclockwise)

Preceding gene: 152976180

Following gene: 152976178

Centisome position: 62.68

GC content: 37.83

Gene sequence:

>2070_bases
ATGAGTAAGATTCGAGTATATGAATATGCAAAGAAGTACAATATTTCAAGTAAAGAGATTATTACAAAACTAAAAGAAAT
GAATATTGAGGTTTCGAATCATATGACAATGTTAGACGATGAAGTAGTAAACAAACTAGATAATGAGTATAATAATGAAG
TGAAAAAACCTTCTGTTGCAGATGAGTTTGAAGTAGAAGAAAAGGTTGTTCGCAGCAAAAAGAACAGCAATAAGAAAAAG
AAAAAAGGCAAAGGAAATCAAGATAAACGTCAAGAGAACTTTGCTGGAAAACAGCAAGCGCAAACTGTAGAAACACCAGA
CAAAATTACTTTCTCTGGAACGCTTACAGTAGGAGAACTTGCGAACAAGCTTGGCAAGGAGCCATCTGAAATTATTAAGA
AGCTCTTTATGCTAGGGATTATGGCAACAATTAACCAAGATCTTGATAAAGATACAATCGAGTTAATTGCAAGTGACTAC
GGTATTGAAGTGGAAGAAGAAGTAGTCATAGATGAAATTGAGTTCGAAACATTTATCGATGAACAAGATGAAGAGGAAGG
GGACTTAAAAGAGCGCCCAGCTGTTGTAACAATTATGGGTCACGTTGACCATGGTAAAACAACATTACTTGACTCTATCC
GTAATTCAAAAGTAACTGCTGGTGAAGCAGGCGGAATTACGCAACATATTGGTGCATACCAAGTAGAAGTAAATGATAAG
AAAATTACATTCTTAGATACTCCAGGTCACGCGGCGTTTACAACGATGCGTGCTCGCGGTGCACAAGTAACGGATATTAC
AATTCTTGTTGTAGCAGCTGATGATGGTGTGATGCCACAAACAGTTGAAGCGATTAACCACGCAAAAGCTGCAGGAGTAC
CAATTATTGTAGCGGTGAATAAAATGGATAAACCAGCAGCAAATCCAGATCGCGTTATGCAAGAATTAACGGAGTATGAG
TTAGTACCAGAAGCTTGGGGTGGCGATACAATCTTCGTACCAATCTCAGCGATTAAAGGTGAAGGAATTGACAATTTATT
AGAAATGATTCTTCTTGTAAGTGAAGTGGAAGAATATAAAGCAAATCCAAATCGCTACGCAACAGGTACTGTAATTGAAG
CACAACTTGACAAAGGAAAAGGTGCAATCGCAACATTACTTGTACAAAATGGTACACTTCGCGTTGGTGATCCAATCGTT
GTTGGTACAACATATGGTCGTGTTCGTGCGATGGTAAATGATATTGGCCGTCGTGTAAAAGTTGCTGGACCATCTACTCC
TGTTGAAATTACAGGTTTAAATGAAGTTCCACAGGCAGGAGATCGTTTTATGGCATTTGCTGACGAGAAAAAAGCACGCC
AAATTGGTGAATCACGTGCACAACAGGCATTGCTTGCACAACGTGGTGAAAAATCTAAATTAAGCCTGGAAGATTTATTC
CAACAAATTCAAGAGGGCGATGTAAAAGAAATTAACTTAATCGTTAAAGCTGATGTACAAGGTTCTGTAGAAGCAATGGC
TGCATCACTTCGCAAAATTGATGTAGAAGGCGTTAAAGTGAAAATTATTCATACAGGCGTAGGTGCGATTACAGAATCTG
ATATTATTTTAGCTTCTGCATCTAATGCAATTGTAATTGGATTTAACGTACGTCCAGATGTAAATGCGAAGCGTACAGCT
GAATCTGAAAATGTTGACATTCGCTTGCATCGTATTATCTATAAAGCAATCGAAGAAATTGAAGCAGCAATGCGCGGTAT
GCTTGATCCAGAATTTGAAGAAAAAGTAATCGGTCAAGCTGAAGTTCGTCAAACGTTTAAAGTAACAAAAGTTGGAACGA
TCGCTGGATGCTATGTAACAGACGGTAAAATTACACGTGATAGTGGCGTTCGTATTATTCGTGACGGTGTTGTCATTTAT
GAAGGACAGCTTGATACATTAAAACGTTTTAAAGATGATGTAAAAGAAGTTGCACAAAACTATGAGTGCGGTATTACAAT
TGAGAAGTACAATGATATTAAAGAAGGAGACATCATTGAAGCATTCATTATGGAAGAAGTGAAGCGATGA

Upstream 100 bases:

>100_bases
GCGATTGGGAGAGACGAACGAGTCGTTGTAGCTGTGTTAGATGAGGGCTTTGCGAAAAAGCTGCGTAGCATGCTCGATAC
AACTTACCGGGGGTGAAGGT

Downstream 100 bases:

>100_bases
TTATCGCTTCGCTCTCATTCGAATGCATCATATACGATGTGCATTCTTTAAAAGAGAAACGAGCAATTTTACAACGTGTG
CTAACCCGTGTGAAACAGCG

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MSKIRVYEYAKKYNISSKEIITKLKEMNIEVSNHMTMLDDEVVNKLDNEYNNEVKKPSVADEFEVEEKVVRSKKNSNKKK
KKGKGNQDKRQENFAGKQQAQTVETPDKITFSGTLTVGELANKLGKEPSEIIKKLFMLGIMATINQDLDKDTIELIASDY
GIEVEEEVVIDEIEFETFIDEQDEEEGDLKERPAVVTIMGHVDHGKTTLLDSIRNSKVTAGEAGGITQHIGAYQVEVNDK
KITFLDTPGHAAFTTMRARGAQVTDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKMDKPAANPDRVMQELTEYE
LVPEAWGGDTIFVPISAIKGEGIDNLLEMILLVSEVEEYKANPNRYATGTVIEAQLDKGKGAIATLLVQNGTLRVGDPIV
VGTTYGRVRAMVNDIGRRVKVAGPSTPVEITGLNEVPQAGDRFMAFADEKKARQIGESRAQQALLAQRGEKSKLSLEDLF
QQIQEGDVKEINLIVKADVQGSVEAMAASLRKIDVEGVKVKIIHTGVGAITESDIILASASNAIVIGFNVRPDVNAKRTA
ESENVDIRLHRIIYKAIEEIEAAMRGMLDPEFEEKVIGQAEVRQTFKVTKVGTIAGCYVTDGKITRDSGVRIIRDGVVIY
EGQLDTLKRFKDDVKEVAQNYECGITIEKYNDIKEGDIIEAFIMEEVKR

Sequences:

>Translated_689_residues
MSKIRVYEYAKKYNISSKEIITKLKEMNIEVSNHMTMLDDEVVNKLDNEYNNEVKKPSVADEFEVEEKVVRSKKNSNKKK
KKGKGNQDKRQENFAGKQQAQTVETPDKITFSGTLTVGELANKLGKEPSEIIKKLFMLGIMATINQDLDKDTIELIASDY
GIEVEEEVVIDEIEFETFIDEQDEEEGDLKERPAVVTIMGHVDHGKTTLLDSIRNSKVTAGEAGGITQHIGAYQVEVNDK
KITFLDTPGHAAFTTMRARGAQVTDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKMDKPAANPDRVMQELTEYE
LVPEAWGGDTIFVPISAIKGEGIDNLLEMILLVSEVEEYKANPNRYATGTVIEAQLDKGKGAIATLLVQNGTLRVGDPIV
VGTTYGRVRAMVNDIGRRVKVAGPSTPVEITGLNEVPQAGDRFMAFADEKKARQIGESRAQQALLAQRGEKSKLSLEDLF
QQIQEGDVKEINLIVKADVQGSVEAMAASLRKIDVEGVKVKIIHTGVGAITESDIILASASNAIVIGFNVRPDVNAKRTA
ESENVDIRLHRIIYKAIEEIEAAMRGMLDPEFEEKVIGQAEVRQTFKVTKVGTIAGCYVTDGKITRDSGVRIIRDGVVIY
EGQLDTLKRFKDDVKEVAQNYECGITIEKYNDIKEGDIIEAFIMEEVKR
>Mature_688_residues
SKIRVYEYAKKYNISSKEIITKLKEMNIEVSNHMTMLDDEVVNKLDNEYNNEVKKPSVADEFEVEEKVVRSKKNSNKKKK
KGKGNQDKRQENFAGKQQAQTVETPDKITFSGTLTVGELANKLGKEPSEIIKKLFMLGIMATINQDLDKDTIELIASDYG
IEVEEEVVIDEIEFETFIDEQDEEEGDLKERPAVVTIMGHVDHGKTTLLDSIRNSKVTAGEAGGITQHIGAYQVEVNDKK
ITFLDTPGHAAFTTMRARGAQVTDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKMDKPAANPDRVMQELTEYEL
VPEAWGGDTIFVPISAIKGEGIDNLLEMILLVSEVEEYKANPNRYATGTVIEAQLDKGKGAIATLLVQNGTLRVGDPIVV
GTTYGRVRAMVNDIGRRVKVAGPSTPVEITGLNEVPQAGDRFMAFADEKKARQIGESRAQQALLAQRGEKSKLSLEDLFQ
QIQEGDVKEINLIVKADVQGSVEAMAASLRKIDVEGVKVKIIHTGVGAITESDIILASASNAIVIGFNVRPDVNAKRTAE
SENVDIRLHRIIYKAIEEIEAAMRGMLDPEFEEKVIGQAEVRQTFKVTKVGTIAGCYVTDGKITRDSGVRIIRDGVVIYE
GQLDTLKRFKDDVKEVAQNYECGITIEKYNDIKEGDIIEAFIMEEVKR

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family

Homologues:

Organism=Homo sapiens, GI53729339, Length=537, Percent_Identity=40.7821229050279, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI53729337, Length=537, Percent_Identity=40.7821229050279, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI84043963, Length=455, Percent_Identity=26.5934065934066, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI157426893, Length=240, Percent_Identity=27.9166666666667, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI34147630, Length=151, Percent_Identity=35.7615894039735, Blast_Score=67, Evalue=6e-11,
Organism=Homo sapiens, GI18390331, Length=142, Percent_Identity=31.6901408450704, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1789559, Length=576, Percent_Identity=55.2083333333333, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI2367247, Length=225, Percent_Identity=32, Blast_Score=83, Evalue=7e-17,
Organism=Escherichia coli, GI1788922, Length=237, Percent_Identity=30.379746835443, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI48994988, Length=137, Percent_Identity=36.4963503649635, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790412, Length=234, Percent_Identity=32.9059829059829, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789737, Length=234, Percent_Identity=32.9059829059829, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71994658, Length=538, Percent_Identity=37.360594795539, Blast_Score=330, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI212656558, Length=567, Percent_Identity=25.9259259259259, Blast_Score=125, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=219, Percent_Identity=31.0502283105023, Blast_Score=84, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556456, Length=253, Percent_Identity=30.8300395256917, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25141371, Length=230, Percent_Identity=28.2608695652174, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324550, Length=614, Percent_Identity=36.4820846905537, Blast_Score=354, Evalue=3e-98,
Organism=Saccharomyces cerevisiae, GI6319282, Length=531, Percent_Identity=26.9303201506591, Blast_Score=131, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6324761, Length=258, Percent_Identity=30.6201550387597, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=283, Percent_Identity=25.4416961130742, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI28572034, Length=532, Percent_Identity=40.4135338345865, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24656849, Length=456, Percent_Identity=27.6315789473684, Blast_Score=129, Evalue=7e-30,
Organism=Drosophila melanogaster, GI160714833, Length=95, Percent_Identity=62.1052631578947, Blast_Score=114, Evalue=3e-25,
Organism=Drosophila melanogaster, GI78706572, Length=224, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281363316, Length=230, Percent_Identity=32.6086956521739, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17864358, Length=230, Percent_Identity=32.6086956521739, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921738, Length=241, Percent_Identity=31.5352697095436, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): IF2_BACCN (A7GRE3)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001375696.1
- ProteinModelPortal:   A7GRE3
- SMR:   A7GRE3
- STRING:   A7GRE3
- EnsemblBacteria:   EBBACT00000038592
- GeneID:   5344192
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_2465
- eggNOG:   COG0532
- GeneTree:   EBGT00070000032011
- HOGENOM:   HBG635083
- OMA:   VRKKRTV
- ProtClustDB:   PRK05306
- BioCyc:   BCER315749:BCER98_2465-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00100_B
- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.40.50.10050
- PANTHER:   PTHR23115:SF41
- TIGRFAMs:   TIGR00487
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N; SSF52156 TIF_IF2_dom3; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 76012; Mature: 75880

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIRVYEYAKKYNISSKEIITKLKEMNIEVSNHMTMLDDEVVNKLDNEYNNEVKKPSVA
CCCEEHHHHHHHCCCCHHHHHHHHHHCCEEHHCCEEHHHHHHHHHHHHHHCCCCCCCCCC
DEFEVEEKVVRSKKNSNKKKKKGKGNQDKRQENFAGKQQAQTVETPDKITFSGTLTVGEL
CHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHCCCHHHHCCCCCCCCEEEEEEEEHHHH
ANKLGKEPSEIIKKLFMLGIMATINQDLDKDTIELIASDYGIEVEEEVVIDEIEFETFID
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHEEEHHHHHHHCC
EQDEEEGDLKERPAVVTIMGHVDHGKTTLLDSIRNSKVTAGEAGGITQHIGAYQVEVNDK
CCCCCCCCHHHCCEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCHHHCCEEEEEECCC
KITFLDTPGHAAFTTMRARGAQVTDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVN
EEEEEECCCCHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEE
KMDKPAANPDRVMQELTEYELVPEAWGGDTIFVPISAIKGEGIDNLLEMILLVSEVEEYK
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHC
ANPNRYATGTVIEAQLDKGKGAIATLLVQNGTLRVGDPIVVGTTYGRVRAMVNDIGRRVK
CCCCCEECCEEEEEEECCCCCEEEEEEEECCEEEECCEEEEECCHHHHHHHHHHHCCEEE
VAGPSTPVEITGLNEVPQAGDRFMAFADEKKARQIGESRAQQALLAQRGEKSKLSLEDLF
ECCCCCCEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
QQIQEGDVKEINLIVKADVQGSVEAMAASLRKIDVEGVKVKIIHTGVGAITESDIILASA
HHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHEECCCEEEEEEEECCCCEECCCEEEEEC
SNAIVIGFNVRPDVNAKRTAESENVDIRLHRIIYKAIEEIEAAMRGMLDPEFEEKVIGQA
CCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHH
EVRQTFKVTKVGTIAGCYVTDGKITRDSGVRIIRDGVVIYEGQLDTLKRFKDDVKEVAQN
HHHHHHEEEEECEEEEEEEECCEEECCCCCEEEECCEEEEECCHHHHHHHHHHHHHHHCC
YECGITIEKYNDIKEGDIIEAFIMEEVKR
CCCCEEEECCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SKIRVYEYAKKYNISSKEIITKLKEMNIEVSNHMTMLDDEVVNKLDNEYNNEVKKPSVA
CCEEHHHHHHHCCCCHHHHHHHHHHCCEEHHCCEEHHHHHHHHHHHHHHCCCCCCCCCC
DEFEVEEKVVRSKKNSNKKKKKGKGNQDKRQENFAGKQQAQTVETPDKITFSGTLTVGEL
CHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHCCCHHHHCCCCCCCCEEEEEEEEHHHH
ANKLGKEPSEIIKKLFMLGIMATINQDLDKDTIELIASDYGIEVEEEVVIDEIEFETFID
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHEEEHHHHHHHCC
EQDEEEGDLKERPAVVTIMGHVDHGKTTLLDSIRNSKVTAGEAGGITQHIGAYQVEVNDK
CCCCCCCCHHHCCEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCHHHCCEEEEEECCC
KITFLDTPGHAAFTTMRARGAQVTDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVN
EEEEEECCCCHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEE
KMDKPAANPDRVMQELTEYELVPEAWGGDTIFVPISAIKGEGIDNLLEMILLVSEVEEYK
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHC
ANPNRYATGTVIEAQLDKGKGAIATLLVQNGTLRVGDPIVVGTTYGRVRAMVNDIGRRVK
CCCCCEECCEEEEEEECCCCCEEEEEEEECCEEEECCEEEEECCHHHHHHHHHHHCCEEE
VAGPSTPVEITGLNEVPQAGDRFMAFADEKKARQIGESRAQQALLAQRGEKSKLSLEDLF
ECCCCCCEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
QQIQEGDVKEINLIVKADVQGSVEAMAASLRKIDVEGVKVKIIHTGVGAITESDIILASA
HHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHEECCCEEEEEEEECCCCEECCCEEEEEC
SNAIVIGFNVRPDVNAKRTAESENVDIRLHRIIYKAIEEIEAAMRGMLDPEFEEKVIGQA
CCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHH
EVRQTFKVTKVGTIAGCYVTDGKITRDSGVRIIRDGVVIYEGQLDTLKRFKDDVKEVAQN
HHHHHHEEEEECEEEEEEEECCEEECCCCCEEEECCEEEEECCHHHHHHHHHHHHHHHCC
YECGITIEKYNDIKEGDIIEAFIMEEVKR
CCCCEEEECCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA