Click here to switch to the map view.

The map label for this gene is truB

Identifier: 152976176

GI number: 152976176

Start: 2557832

End: 2558755

Strand: Reverse

Name: truB

Synonym: Bcer98_2462

Alternate gene names: 152976176

Gene position: 2558755-2557832 (Counterclockwise)

Preceding gene: 152976177

Following gene: 152976175

Centisome position: 62.61

GC content: 36.58

Gene sequence:

>924_bases
ATGGAAGGTGTAGTATTATTACATAAACCAAAAGGAATGACTTCACATGATTGTGTATTTAAGTTACGAAAAATATTACG
TGAAAAGCGAATTGGTCACACAGGTACATTAGATCCAGATGTAACAGGAGTGTTACCGATTTGTGTGGGACGTGCTACAA
AGATTGCACAATTTTTGACGAGTGAAACAAAAACTTATGAGGGGGAAGTTACATTAGGATTTTCAACTACCACTGAAGAT
GCTTCTGGTGAAGTAGTTGAAAAACAAGATGTAAATCGCACCATTACACGCAAGGAAATTGAAGCGGTACTAGCAGAATT
GACAGGTACGCTTGAACAAGTGCCACCTATGTATTCGGCTGTAAAGGTGAATGGGAAAAAATTATATGAGTATGCGAGAG
CTGGGCAAGAAGTGAAGCGCCCAGTTCGCATCATTACAATTCATGAGTTTACACTACTGGACAACCGTGAAACATTTGAA
GGTGCAAATATTTCATTCCGCTTCCGTGTTACATGTAGCAAAGGAACGTATGTGAGAACATTAGCAGTTATGATCGGAGA
AAAATTAGGATTCCCGGCACATATGTCTGATCTTGTTAGAACAGCTTCAGGAGAATTTCAGCTACATGACTGCGTATCAT
TTGAAGAAATTGAGGAAAACATGCAAAATGGAACAGTAGAGTCTGTTTTTATTTCAATTGATGAAGCGTTAAGCAAGTTT
CCAAAAATAGTGGTAGACGAAAAACAAGCAGAAAAAATAAAAAACGGTATGTTTTTAAAAAATGAAATACAAGCGGAAAC
TCCATTTATTACAGTATTTGATCGTAATAATCATTGTTTAGCGATTTATGAACATCACCCAAAACGTCCAGGCATGTTAA
AGCCAATGAAAGTACTTGTGAATAATCAAGAATTAAAGCTATAA

Upstream 100 bases:

>100_bases
TTTTCAATAGAAAGTTTGTTTTACATACTCGTGTAAGTAGACAAGCATAGTAAAAAATATCTACTTAATTTTCGCAAGAA
GATAAGAATGAGGTGAACAT

Downstream 100 bases:

>100_bases
TGAAGTATTAGAAGGATTATTACAGAAAAAGGTGAACGCAAGCGTGAAACTCATTCATTTAACTCATCGCCATGATTTAA
ATAAACTAGAATTACCACCT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MEGVVLLHKPKGMTSHDCVFKLRKILREKRIGHTGTLDPDVTGVLPICVGRATKIAQFLTSETKTYEGEVTLGFSTTTED
ASGEVVEKQDVNRTITRKEIEAVLAELTGTLEQVPPMYSAVKVNGKKLYEYARAGQEVKRPVRIITIHEFTLLDNRETFE
GANISFRFRVTCSKGTYVRTLAVMIGEKLGFPAHMSDLVRTASGEFQLHDCVSFEEIEENMQNGTVESVFISIDEALSKF
PKIVVDEKQAEKIKNGMFLKNEIQAETPFITVFDRNNHCLAIYEHHPKRPGMLKPMKVLVNNQELKL

Sequences:

>Translated_307_residues
MEGVVLLHKPKGMTSHDCVFKLRKILREKRIGHTGTLDPDVTGVLPICVGRATKIAQFLTSETKTYEGEVTLGFSTTTED
ASGEVVEKQDVNRTITRKEIEAVLAELTGTLEQVPPMYSAVKVNGKKLYEYARAGQEVKRPVRIITIHEFTLLDNRETFE
GANISFRFRVTCSKGTYVRTLAVMIGEKLGFPAHMSDLVRTASGEFQLHDCVSFEEIEENMQNGTVESVFISIDEALSKF
PKIVVDEKQAEKIKNGMFLKNEIQAETPFITVFDRNNHCLAIYEHHPKRPGMLKPMKVLVNNQELKL
>Mature_307_residues
MEGVVLLHKPKGMTSHDCVFKLRKILREKRIGHTGTLDPDVTGVLPICVGRATKIAQFLTSETKTYEGEVTLGFSTTTED
ASGEVVEKQDVNRTITRKEIEAVLAELTGTLEQVPPMYSAVKVNGKKLYEYARAGQEVKRPVRIITIHEFTLLDNRETFE
GANISFRFRVTCSKGTYVRTLAVMIGEKLGFPAHMSDLVRTASGEFQLHDCVSFEEIEENMQNGTVESVFISIDEALSKF
PKIVVDEKQAEKIKNGMFLKNEIQAETPFITVFDRNNHCLAIYEHHPKRPGMLKPMKVLVNNQELKL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=258, Percent_Identity=32.5581395348837, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI215599015, Length=262, Percent_Identity=28.6259541984733, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI4503337, Length=262, Percent_Identity=28.6259541984733, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI2367200, Length=265, Percent_Identity=38.4905660377359, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17553978, Length=202, Percent_Identity=31.1881188118812, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323204, Length=265, Percent_Identity=30.9433962264151, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324037, Length=138, Percent_Identity=39.1304347826087, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI281364189, Length=228, Percent_Identity=31.5789473684211, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281364187, Length=228, Percent_Identity=31.5789473684211, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281364185, Length=228, Percent_Identity=31.5789473684211, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281364183, Length=228, Percent_Identity=31.5789473684211, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI62471759, Length=228, Percent_Identity=31.5789473684211, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17975520, Length=228, Percent_Identity=31.5789473684211, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_BACCN (A7GRE0)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001375693.1
- ProteinModelPortal:   A7GRE0
- SMR:   A7GRE0
- STRING:   A7GRE0
- EnsemblBacteria:   EBBACT00000037431
- GeneID:   5344180
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_2462
- eggNOG:   COG0130
- GeneTree:   EBGT00050000002629
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- ProtClustDB:   PRK01550
- BioCyc:   BCER315749:BCER98_2462-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 34589; Mature: 34589

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGVVLLHKPKGMTSHDCVFKLRKILREKRIGHTGTLDPDVTGVLPICVGRATKIAQFLT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHH
SETKTYEGEVTLGFSTTTEDASGEVVEKQDVNRTITRKEIEAVLAELTGTLEQVPPMYSA
HCCCEECCEEEEEEECCCCCCCCCEECHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHE
VKVNGKKLYEYARAGQEVKRPVRIITIHEFTLLDNRETFEGANISFRFRVTCSKGTYVRT
EEECHHHHHHHHHCCHHHCCCEEEEEEEEEEEECCCCCCCCCCEEEEEEEEECCCCHHHH
LAVMIGEKLGFPAHMSDLVRTASGEFQLHDCVSFEEIEENMQNGTVESVFISIDEALSKF
HHHHHHHHCCCCHHHHHHHHHCCCCEEEHHCCCHHHHHHHHHCCCHHHEEEEHHHHHHHC
PKIVVDEKQAEKIKNGMFLKNEIQAETPFITVFDRNNHCLAIYEHHPKRPGMLKPMKVLV
CCEEECCHHHHHHHCCCEEEHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCHHHHEE
NNQELKL
CCCCCCC
>Mature Secondary Structure
MEGVVLLHKPKGMTSHDCVFKLRKILREKRIGHTGTLDPDVTGVLPICVGRATKIAQFLT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHH
SETKTYEGEVTLGFSTTTEDASGEVVEKQDVNRTITRKEIEAVLAELTGTLEQVPPMYSA
HCCCEECCEEEEEEECCCCCCCCCEECHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHE
VKVNGKKLYEYARAGQEVKRPVRIITIHEFTLLDNRETFEGANISFRFRVTCSKGTYVRT
EEECHHHHHHHHHCCHHHCCCEEEEEEEEEEEECCCCCCCCCCEEEEEEEEECCCCHHHH
LAVMIGEKLGFPAHMSDLVRTASGEFQLHDCVSFEEIEENMQNGTVESVFISIDEALSKF
HHHHHHHHCCCCHHHHHHHHHCCCCEEEHHCCCHHHHHHHHHCCCHHHEEEEHHHHHHHC
PKIVVDEKQAEKIKNGMFLKNEIQAETPFITVFDRNNHCLAIYEHHPKRPGMLKPMKVLV
CCEEECCHHHHHHHCCCEEEHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCHHHHEE
NNQELKL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA