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The map label for this gene is dapG [H]

Identifier: 152976166

GI number: 152976166

Start: 2547107

End: 2548339

Strand: Reverse

Name: dapG [H]

Synonym: Bcer98_2452

Alternate gene names: 152976166

Gene position: 2548339-2547107 (Counterclockwise)

Preceding gene: 152976167

Following gene: 152976165

Centisome position: 62.35

GC content: 39.74

Gene sequence:

>1233_bases
ATGAAAATTATTGTTCAAAAATTCGGTGGGACATCAGTACGTGATGAAAATGGACGTAAGCATGCACTTCACCATATTAA
AAAATCATTAGATGCTGGCTATAAAGTAGTTACGGTTGTATCAGCAATGGGACGAAAAGGCGAGCCATATGCGACAGATA
CATTATTAAGTCTTGTGAATCAAGAGAAATGCAGTATTTCAAAACGTGAGCAAGATTTATTGTTATCATGTGGAGAATTA
ATCTCAGCTGTCGTCTTCTCAAATATGCTAAATGAAAATGGAATAAAAGCAGCGGCGCTAAATGGTGCGCAAGCTGGTTT
TGTAACGAATGACGACTTTACGAATGCAAAGATTATTGAAATGAATTGCGATCGTATATATGAAGAGTTACAAAATGTAG
ATGTTATTGTCGTTACAGGATTTCAAGGTCAAACGAAAGCTGGAGATACGACAACGCTTGGACGTGGAGGCAGTGATACA
TCCGCTTCCGCATTAGGTGTTGCACTTCATGCGGAATATATAGATATATTTACAGATGTAGAAGGGGTAATGACGGCAGA
TCCACGTATTGTAAAAGATGCACGTCATCTTCAAACTGTAACATACAATGAAATTTGTAACATGGCATATCAAGGGGCAA
AAGTTGTTCATCCGCGCGCCGTTGAAATTGCGATGCATGCGAAAGTGCCGCTTCGCGTACGTTCTACGTACTCTGATAGC
GAAGGTACGCTTATTGCAGCATATGATGGTGCTACGAAAGGGCGCGATGTAGAGGAACGTCCTGTTACAGGAATTGCTCA
CGTATCGAATGTGACACAAATTAAAGTGCTTGCGAAAGATGGAGCATATGATTTACAGCAACAAGTTTTTAAAGAGATGG
CGAGTGAAGGGATTAGTGTTGATTTAATTAATATTTCCCCAACAGGTGTGGCGTATACAGTGAGCGATCATGTCTCAGGT
CGTGCGATTGAATTATTAAGAACACTTGGATATGAACCAATTGTAACGGAACATTGTGCAAAAGTATCCATTGTTGGAGC
TGGAATGGCTGGCATTCCAGGGGTTACTGCCAAAATTGTTACTGCATTAGCAGAAAAAGGTATTCAAATTTTACAATCTG
CAGATAGCCATACGACGATTTGGGTGCTTGTAAAAGAGACAGATTTAGTAGAGGCTGTAAATGCATTACATAGTGCATTT
GAGCTTTCTAAAGAAAAGCAACTGGAACAATAA

Upstream 100 bases:

>100_bases
TCTGATAACTTATTAAAAGGCGCCGCATGGAACTCTGTTCAAATTGCAGAACGATTAATCAAATTACAATTAGTGTAAAC
GGCTAAGAGAAGGTGCAAAG

Downstream 100 bases:

>100_bases
GGAGTGAGACCATGATAGATTTTGGGACAATTGCAACTGCGATGGTAACGCCGTTTGATAAAAACGGAAATATCGATTTT
GCAAAGACAACGAAATTGGT

Product: aspartate kinase I

Products: NA

Alternate protein names: Aspartate kinase 1; Aspartokinase I [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKIIVQKFGGTSVRDENGRKHALHHIKKSLDAGYKVVTVVSAMGRKGEPYATDTLLSLVNQEKCSISKREQDLLLSCGEL
ISAVVFSNMLNENGIKAAALNGAQAGFVTNDDFTNAKIIEMNCDRIYEELQNVDVIVVTGFQGQTKAGDTTTLGRGGSDT
SASALGVALHAEYIDIFTDVEGVMTADPRIVKDARHLQTVTYNEICNMAYQGAKVVHPRAVEIAMHAKVPLRVRSTYSDS
EGTLIAAYDGATKGRDVEERPVTGIAHVSNVTQIKVLAKDGAYDLQQQVFKEMASEGISVDLINISPTGVAYTVSDHVSG
RAIELLRTLGYEPIVTEHCAKVSIVGAGMAGIPGVTAKIVTALAEKGIQILQSADSHTTIWVLVKETDLVEAVNALHSAF
ELSKEKQLEQ

Sequences:

>Translated_410_residues
MKIIVQKFGGTSVRDENGRKHALHHIKKSLDAGYKVVTVVSAMGRKGEPYATDTLLSLVNQEKCSISKREQDLLLSCGEL
ISAVVFSNMLNENGIKAAALNGAQAGFVTNDDFTNAKIIEMNCDRIYEELQNVDVIVVTGFQGQTKAGDTTTLGRGGSDT
SASALGVALHAEYIDIFTDVEGVMTADPRIVKDARHLQTVTYNEICNMAYQGAKVVHPRAVEIAMHAKVPLRVRSTYSDS
EGTLIAAYDGATKGRDVEERPVTGIAHVSNVTQIKVLAKDGAYDLQQQVFKEMASEGISVDLINISPTGVAYTVSDHVSG
RAIELLRTLGYEPIVTEHCAKVSIVGAGMAGIPGVTAKIVTALAEKGIQILQSADSHTTIWVLVKETDLVEAVNALHSAF
ELSKEKQLEQ
>Mature_410_residues
MKIIVQKFGGTSVRDENGRKHALHHIKKSLDAGYKVVTVVSAMGRKGEPYATDTLLSLVNQEKCSISKREQDLLLSCGEL
ISAVVFSNMLNENGIKAAALNGAQAGFVTNDDFTNAKIIEMNCDRIYEELQNVDVIVVTGFQGQTKAGDTTTLGRGGSDT
SASALGVALHAEYIDIFTDVEGVMTADPRIVKDARHLQTVTYNEICNMAYQGAKVVHPRAVEIAMHAKVPLRVRSTYSDS
EGTLIAAYDGATKGRDVEERPVTGIAHVSNVTQIKVLAKDGAYDLQQQVFKEMASEGISVDLINISPTGVAYTVSDHVSG
RAIELLRTLGYEPIVTEHCAKVSIVGAGMAGIPGVTAKIVTALAEKGIQILQSADSHTTIWVLVKETDLVEAVNALHSAF
ELSKEKQLEQ

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartokinase family [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=279, Percent_Identity=34.4086021505376, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1790455, Length=342, Percent_Identity=29.5321637426901, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1790376, Length=172, Percent_Identity=33.7209302325581, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320893, Length=392, Percent_Identity=27.2959183673469, Blast_Score=129, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 44084; Mature: 44084

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIVQKFGGTSVRDENGRKHALHHIKKSLDAGYKVVTVVSAMGRKGEPYATDTLLSLVN
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
QEKCSISKREQDLLLSCGELISAVVFSNMLNENGIKAAALNGAQAGFVTNDDFTNAKIIE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEECCCCCCCEEEE
MNCDRIYEELQNVDVIVVTGFQGQTKAGDTTTLGRGGSDTSASALGVALHAEYIDIFTDV
ECHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
EGVMTADPRIVKDARHLQTVTYNEICNMAYQGAKVVHPRAVEIAMHAKVPLRVRSTYSDS
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEEECCCCEEEECCCCCC
EGTLIAAYDGATKGRDVEERPVTGIAHVSNVTQIKVLAKDGAYDLQQQVFKEMASEGISV
CCCEEEEECCCCCCCCCCCCCCCCHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEE
DLINISPTGVAYTVSDHVSGRAIELLRTLGYEPIVTEHCAKVSIVGAGMAGIPGVTAKIV
EEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCHHHHHHCEEEEEECCCCCCCCHHHHHH
TALAEKGIQILQSADSHTTIWVLVKETDLVEAVNALHSAFELSKEKQLEQ
HHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIIVQKFGGTSVRDENGRKHALHHIKKSLDAGYKVVTVVSAMGRKGEPYATDTLLSLVN
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
QEKCSISKREQDLLLSCGELISAVVFSNMLNENGIKAAALNGAQAGFVTNDDFTNAKIIE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEECCCCCCCEEEE
MNCDRIYEELQNVDVIVVTGFQGQTKAGDTTTLGRGGSDTSASALGVALHAEYIDIFTDV
ECHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
EGVMTADPRIVKDARHLQTVTYNEICNMAYQGAKVVHPRAVEIAMHAKVPLRVRSTYSDS
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEEECCCCEEEECCCCCC
EGTLIAAYDGATKGRDVEERPVTGIAHVSNVTQIKVLAKDGAYDLQQQVFKEMASEGISV
CCCEEEEECCCCCCCCCCCCCCCCHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEE
DLINISPTGVAYTVSDHVSGRAIELLRTLGYEPIVTEHCAKVSIVGAGMAGIPGVTAKIV
EEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCHHHHHHCEEEEEECCCCCCCCHHHHHH
TALAEKGIQILQSADSHTTIWVLVKETDLVEAVNALHSAFELSKEKQLEQ
HHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8098035; 9384377 [H]