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The map label for this gene is pepT

Identifier: 152976127

GI number: 152976127

Start: 2496442

End: 2497674

Strand: Reverse

Name: pepT

Synonym: Bcer98_2411

Alternate gene names: 152976127

Gene position: 2497674-2496442 (Counterclockwise)

Preceding gene: 152976128

Following gene: 152976126

Centisome position: 61.11

GC content: 35.44

Gene sequence:

>1233_bases
ATGAGACAAGAATTAATAGAAAGATTTACACGATATGTAAAAATTGATACACAATCGAATGAAGAAAGCCATACAGTACC
ATCAACACCAGGGCAAATTGAGTTTGGAAAACAATTAGTTGAAGAATTAAAACAAATTGGTTTGATTGAAGTAACGATGG
ATGAAAATGGATATGTCATGGCAACACTTCCTGCCAATACAGATAAAGATGTTCCTGTAATTGGATTTTTAGCTCATTTA
GATACAGCAACTGATTTTACCGGGAAGAATGTAAAGCCGCAAATTCATGAAAATTTTGATGGGAAAGCTATTACATTAAA
CGAAGAATTAAATGTCGTATTAACACCAGAGCAGTTTCCAGAGTTGCCGTCTTATAAAGGACATACTCTTATTACAACTG
ATGGAACGACGCTTCTTGGTGCGGATGATAAAGCTGGTTTAACAGAAATTATGACTGCGATGAATTATTTGATACATAAT
CCACAAATTAAGCACGGAAAGATTAGAGTGGCATTTACACCGGATGAAGAGATTGGACGCGGACCTTCTCATTTTGATGT
GAAAGCGTTTGGAGCATCTTTTGCTTATACAATGGATGGAGGCCCACTTGGAGGATTAGAATATGAGAGTTTCAATGCTG
CAAGTGCTAAACTCACTTTTAAAGGGAATAATACGCATCCAGGTACAGCAAAAAATAAAATGTTAAACGCTAGTAAGATA
GCGATGGAATTCCACAGTCAGTTACCGGCCGAAGAAGCACCTGAGTATACGGAGGGGTATGAAGGGTTTTATCATCTTCT
TTCTTTAAATGGAGATGTAGAGCAAAGTAAAGCATATTACATTATTCGTGATTTTGATCGCGGTAATTTTGAAGCTCGAA
AAAATAAAATCAAGCAAATTGTAGAGAAAATGCAAGAAAAATATGGCGAAGAAAATATTATTCTTGAGATGAAAGATCAA
TATTATAATATGCTTGAGAAGATCGAACCAGTAAAAGAGATTGTGGATATTGCTTATGATGCGATGAAAAATTTAAATAT
TGAGCCAAATATTCAGCCGATTCGCGGAGGAACAGATGGTTCTCAATTATCGTATATGGGATTACCGACTCCAAATATTT
TTACAGGGGGAGAAAATTACCATGGAAAATTTGAGTATGTTTCTGTAGATAATATGGAAAAAGCTACTCATGTCATTATA
GAAATAGCACGGTTGTTTGAAGAACGAGCGTAA

Upstream 100 bases:

>100_bases
ATATGTGTGGCAAAATGAGATGAATACAGTCAAAAAAGTTAAAAAATTTAGTTTTAAGGATTATAATAGAAGGAGATGGA
GAAAAAGGGAGTGACGATAG

Downstream 100 bases:

>100_bases
GAAAGAAATCCAGGTAGTAAAATAAAATTTGCTATCTGGATTTTTTACCATATACAAAGGGGATAGGGACCATGAATCAA
GATGAGCTAATAGGGTTTAT

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MRQELIERFTRYVKIDTQSNEESHTVPSTPGQIEFGKQLVEELKQIGLIEVTMDENGYVMATLPANTDKDVPVIGFLAHL
DTATDFTGKNVKPQIHENFDGKAITLNEELNVVLTPEQFPELPSYKGHTLITTDGTTLLGADDKAGLTEIMTAMNYLIHN
PQIKHGKIRVAFTPDEEIGRGPSHFDVKAFGASFAYTMDGGPLGGLEYESFNAASAKLTFKGNNTHPGTAKNKMLNASKI
AMEFHSQLPAEEAPEYTEGYEGFYHLLSLNGDVEQSKAYYIIRDFDRGNFEARKNKIKQIVEKMQEKYGEENIILEMKDQ
YYNMLEKIEPVKEIVDIAYDAMKNLNIEPNIQPIRGGTDGSQLSYMGLPTPNIFTGGENYHGKFEYVSVDNMEKATHVII
EIARLFEERA

Sequences:

>Translated_410_residues
MRQELIERFTRYVKIDTQSNEESHTVPSTPGQIEFGKQLVEELKQIGLIEVTMDENGYVMATLPANTDKDVPVIGFLAHL
DTATDFTGKNVKPQIHENFDGKAITLNEELNVVLTPEQFPELPSYKGHTLITTDGTTLLGADDKAGLTEIMTAMNYLIHN
PQIKHGKIRVAFTPDEEIGRGPSHFDVKAFGASFAYTMDGGPLGGLEYESFNAASAKLTFKGNNTHPGTAKNKMLNASKI
AMEFHSQLPAEEAPEYTEGYEGFYHLLSLNGDVEQSKAYYIIRDFDRGNFEARKNKIKQIVEKMQEKYGEENIILEMKDQ
YYNMLEKIEPVKEIVDIAYDAMKNLNIEPNIQPIRGGTDGSQLSYMGLPTPNIFTGGENYHGKFEYVSVDNMEKATHVII
EIARLFEERA
>Mature_410_residues
MRQELIERFTRYVKIDTQSNEESHTVPSTPGQIEFGKQLVEELKQIGLIEVTMDENGYVMATLPANTDKDVPVIGFLAHL
DTATDFTGKNVKPQIHENFDGKAITLNEELNVVLTPEQFPELPSYKGHTLITTDGTTLLGADDKAGLTEIMTAMNYLIHN
PQIKHGKIRVAFTPDEEIGRGPSHFDVKAFGASFAYTMDGGPLGGLEYESFNAASAKLTFKGNNTHPGTAKNKMLNASKI
AMEFHSQLPAEEAPEYTEGYEGFYHLLSLNGDVEQSKAYYIIRDFDRGNFEARKNKIKQIVEKMQEKYGEENIILEMKDQ
YYNMLEKIEPVKEIVDIAYDAMKNLNIEPNIQPIRGGTDGSQLSYMGLPTPNIFTGGENYHGKFEYVSVDNMEKATHVII
EIARLFEERA

Specific function: Cleaves the N-terminal amino acid of tripeptides

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family

Homologues:

Organism=Escherichia coli, GI1787372, Length=407, Percent_Identity=55.5282555282555, Blast_Score=454, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEPT_BACCN (A7GR91)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001375644.1
- ProteinModelPortal:   A7GR91
- SMR:   A7GR91
- STRING:   A7GR91
- MEROPS:   M20.003
- EnsemblBacteria:   EBBACT00000039170
- GeneID:   5346926
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_2411
- eggNOG:   COG2195
- GeneTree:   EBGT00050000000947
- HOGENOM:   HBG288638
- OMA:   HMDTAPD
- ProtClustDB:   PRK05469
- BioCyc:   BCER315749:BCER98_2411-MONOMER
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00550
- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161
- PIRSF:   PIRSF037215
- TIGRFAMs:   TIGR01882

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.4.11.4

Molecular weight: Translated: 46053; Mature: 46053

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 81-81 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQELIERFTRYVKIDTQSNEESHTVPSTPGQIEFGKQLVEELKQIGLIEVTMDENGYVM
CHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCEEE
ATLPANTDKDVPVIGFLAHLDTATDFTGKNVKPQIHENFDGKAITLNEELNVVLTPEQFP
EEECCCCCCCCCCEEHHHHHCCCCCCCCCCCCCHHHCCCCCCEEEECCCCEEEECCHHCC
ELPSYKGHTLITTDGTTLLGADDKAGLTEIMTAMNYLIHNPQIKHGKIRVAFTPDEEIGR
CCCCCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHCC
GPSHFDVKAFGASFAYTMDGGPLGGLEYESFNAASAKLTFKGNNTHPGTAKNKMLNASKI
CCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHCCHHHH
AMEFHSQLPAEEAPEYTEGYEGFYHLLSLNGDVEQSKAYYIIRDFDRGNFEARKNKIKQI
HHHHHHHCCHHCCCCHHHHHHHEEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
VEKMQEKYGEENIILEMKDQYYNMLEKIEPVKEIVDIAYDAMKNLNIEPNIQPIRGGTDG
HHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCC
SQLSYMGLPTPNIFTGGENYHGKFEYVSVDNMEKATHVIIEIARLFEERA
CCEEEECCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRQELIERFTRYVKIDTQSNEESHTVPSTPGQIEFGKQLVEELKQIGLIEVTMDENGYVM
CHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCEEE
ATLPANTDKDVPVIGFLAHLDTATDFTGKNVKPQIHENFDGKAITLNEELNVVLTPEQFP
EEECCCCCCCCCCEEHHHHHCCCCCCCCCCCCCHHHCCCCCCEEEECCCCEEEECCHHCC
ELPSYKGHTLITTDGTTLLGADDKAGLTEIMTAMNYLIHNPQIKHGKIRVAFTPDEEIGR
CCCCCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHCC
GPSHFDVKAFGASFAYTMDGGPLGGLEYESFNAASAKLTFKGNNTHPGTAKNKMLNASKI
CCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHCCHHHH
AMEFHSQLPAEEAPEYTEGYEGFYHLLSLNGDVEQSKAYYIIRDFDRGNFEARKNKIKQI
HHHHHHHCCHHCCCCHHHHHHHEEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
VEKMQEKYGEENIILEMKDQYYNMLEKIEPVKEIVDIAYDAMKNLNIEPNIQPIRGGTDG
HHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCC
SQLSYMGLPTPNIFTGGENYHGKFEYVSVDNMEKATHVIIEIARLFEERA
CCEEEECCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA