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The map label for this gene is pbpE [H]

Identifier: 152976080

GI number: 152976080

Start: 2448263

End: 2449408

Strand: Reverse

Name: pbpE [H]

Synonym: Bcer98_2357

Alternate gene names: 152976080

Gene position: 2449408-2448263 (Counterclockwise)

Preceding gene: 152976083

Following gene: 152976073

Centisome position: 59.93

GC content: 32.37

Gene sequence:

>1146_bases
ATGAATGGACAAAATTTGAAGCGTAAAGAGCTAGATAAGGTATTAGATTTCTTAGAACAAAATAGGCTATTTAGCGGGGT
GATATTATTATCTGAGGAAGGTAAAGCATTTTACAAGAGAGCAATTGGCAAGAGAAATAATTCAAGTCAACAGTCTCTTG
ATGCAGTATTCGAAATTGCCTCGATTTCGAAGAGCTTTACAGCAATGGCAATTATGATACTAATTGAGGAGAAAAAACTA
GAGCTAGATGATAATCTTAAAAAGTTTTTCTCTTTTCTTTCTTATGAAAACATTACTATTAAAAATCTGTTAAATCACAC
TTCTGGACTTCCAGAGTACATGGAATGGTTTGAAAATCCAGCTAACTGGAATCCTAATAGAATTGCAACGAATAAAGATA
TAGTTGAATTTTTGAAAGTTGAGAAGCCCGAAGTGTTATTTCATGTGAATGAAAAGTGGGAGTATTGTAATACTGGTTAT
GTATTACTTGCAGAAATCATAGAAATGGTTGCTAAAATGGGGTACGGAGAGTTTTTACAGAAAAGAATATTTACACCTTT
AAATATGTTCAATACATCAACACATTCACAATTTTTAAATAACGACATTGAAAATTTTTCGTTAGGTTTTATCTATGATT
GGGAACAGGACTTGTATAGGCTTCCAAATGAAATGGAAGAACACAAGTATGTTTACTTCTTCGATGGTGTTAAAGGTGAT
GGAGGTATAAAATCCAACGTAGATGATATGCTGAAATGGGAGCGTGCTATTTATCATAATGACCTAGTATCAGAACAAAC
CAAGGAAAGTATTATAGATTCAGTTTTCATTAAAGGAGAAGCTGCGAATGGATATTGTCCATGTTTGCATGAAAATTCCG
GGGGATATGGACTTGGTTGGAAAATAGAAAACCATCCTCAATATAAGAAGATTATTCTTCACGATGGATATTGGGCAGGT
TATTATTCAGTTTTAATTAGTTATAAGGATAGTAACAAAGCTATAGTAATGTTAAATAACCTAGACTTTACGAATAACGA
GTTAAACAAGATACCATATTTGTTAACTCTTACTTTAGAAAAGATTTTATTCGGTGAAAAAGTAGGGTTACAAGAGTTTG
AGCAGCTAATAATGTCAAATCGTTAG

Upstream 100 bases:

>100_bases
AATATTTTCAACTTTATATAATCAACAATAAGAGGTACGCCTCTCTGATAATAATGTATCTCATATTAAATACAAAAATC
ATAAATGAGGTCATTGAATT

Downstream 100 bases:

>100_bases
AATAAGCAATTGGGATTATTTCGTTAAAGGGCAATTTGATTCTTTTAATGGAGCAATGATTATAAAAAATGTCAAAATCT
TTTTAAAAGATACTTTTTCT

Product: beta-lactamase

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MNGQNLKRKELDKVLDFLEQNRLFSGVILLSEEGKAFYKRAIGKRNNSSQQSLDAVFEIASISKSFTAMAIMILIEEKKL
ELDDNLKKFFSFLSYENITIKNLLNHTSGLPEYMEWFENPANWNPNRIATNKDIVEFLKVEKPEVLFHVNEKWEYCNTGY
VLLAEIIEMVAKMGYGEFLQKRIFTPLNMFNTSTHSQFLNNDIENFSLGFIYDWEQDLYRLPNEMEEHKYVYFFDGVKGD
GGIKSNVDDMLKWERAIYHNDLVSEQTKESIIDSVFIKGEAANGYCPCLHENSGGYGLGWKIENHPQYKKIILHDGYWAG
YYSVLISYKDSNKAIVMLNNLDFTNNELNKIPYLLTLTLEKILFGEKVGLQEFEQLIMSNR

Sequences:

>Translated_381_residues
MNGQNLKRKELDKVLDFLEQNRLFSGVILLSEEGKAFYKRAIGKRNNSSQQSLDAVFEIASISKSFTAMAIMILIEEKKL
ELDDNLKKFFSFLSYENITIKNLLNHTSGLPEYMEWFENPANWNPNRIATNKDIVEFLKVEKPEVLFHVNEKWEYCNTGY
VLLAEIIEMVAKMGYGEFLQKRIFTPLNMFNTSTHSQFLNNDIENFSLGFIYDWEQDLYRLPNEMEEHKYVYFFDGVKGD
GGIKSNVDDMLKWERAIYHNDLVSEQTKESIIDSVFIKGEAANGYCPCLHENSGGYGLGWKIENHPQYKKIILHDGYWAG
YYSVLISYKDSNKAIVMLNNLDFTNNELNKIPYLLTLTLEKILFGEKVGLQEFEQLIMSNR
>Mature_381_residues
MNGQNLKRKELDKVLDFLEQNRLFSGVILLSEEGKAFYKRAIGKRNNSSQQSLDAVFEIASISKSFTAMAIMILIEEKKL
ELDDNLKKFFSFLSYENITIKNLLNHTSGLPEYMEWFENPANWNPNRIATNKDIVEFLKVEKPEVLFHVNEKWEYCNTGY
VLLAEIIEMVAKMGYGEFLQKRIFTPLNMFNTSTHSQFLNNDIENFSLGFIYDWEQDLYRLPNEMEEHKYVYFFDGVKGD
GGIKSNVDDMLKWERAIYHNDLVSEQTKESIIDSVFIKGEAANGYCPCLHENSGGYGLGWKIENHPQYKKIILHDGYWAG
YYSVLISYKDSNKAIVMLNNLDFTNNELNKIPYLLTLTLEKILFGEKVGLQEFEQLIMSNR

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI115533026, Length=376, Percent_Identity=25.7978723404255, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17550934, Length=149, Percent_Identity=30.8724832214765, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 44361; Mature: 44361

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGQNLKRKELDKVLDFLEQNRLFSGVILLSEEGKAFYKRAIGKRNNSSQQSLDAVFEIA
CCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHH
SISKSFTAMAIMILIEEKKLELDDNLKKFFSFLSYENITIKNLLNHTSGLPEYMEWFENP
HHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHCCC
ANWNPNRIATNKDIVEFLKVEKPEVLFHVNEKWEYCNTGYVLLAEIIEMVAKMGYGEFLQ
CCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHH
KRIFTPLNMFNTSTHSQFLNNDIENFSLGFIYDWEQDLYRLPNEMEEHKYVYFFDGVKGD
HHHCCCHHHHCCCHHHHHHHCCCCCCEEEEEECCHHHHHHCCCHHHHCCEEEEECCCCCC
GGIKSNVDDMLKWERAIYHNDLVSEQTKESIIDSVFIKGEAANGYCPCLHENSGGYGLGW
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEECCCCCEEECE
KIENHPQYKKIILHDGYWAGYYSVLISYKDSNKAIVMLNNLDFTNNELNKIPYLLTLTLE
EECCCCCEEEEEEECCCHHEEEEEEEEEECCCCEEEEEECCCCCCCHHHHCCCHHHHHHH
KILFGEKVGLQEFEQLIMSNR
HHHHCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MNGQNLKRKELDKVLDFLEQNRLFSGVILLSEEGKAFYKRAIGKRNNSSQQSLDAVFEIA
CCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHH
SISKSFTAMAIMILIEEKKLELDDNLKKFFSFLSYENITIKNLLNHTSGLPEYMEWFENP
HHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHCCC
ANWNPNRIATNKDIVEFLKVEKPEVLFHVNEKWEYCNTGYVLLAEIIEMVAKMGYGEFLQ
CCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHH
KRIFTPLNMFNTSTHSQFLNNDIENFSLGFIYDWEQDLYRLPNEMEEHKYVYFFDGVKGD
HHHCCCHHHHCCCHHHHHHHCCCCCCEEEEEECCHHHHHHCCCHHHHCCEEEEECCCCCC
GGIKSNVDDMLKWERAIYHNDLVSEQTKESIIDSVFIKGEAANGYCPCLHENSGGYGLGW
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEECCCCCEEECE
KIENHPQYKKIILHDGYWAGYYSVLISYKDSNKAIVMLNNLDFTNNELNKIPYLLTLTLE
EECCCCCEEEEEEECCCHHEEEEEEEEEECCCCEEEEEECCCCCCCHHHHCCCHHHHHHH
KILFGEKVGLQEFEQLIMSNR
HHHHCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]