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The map label for this gene is alkK [H]

Identifier: 152976000

GI number: 152976000

Start: 2371555

End: 2373156

Strand: Reverse

Name: alkK [H]

Synonym: Bcer98_2273

Alternate gene names: 152976000

Gene position: 2373156-2371555 (Counterclockwise)

Preceding gene: 152976001

Following gene: 152975998

Centisome position: 58.07

GC content: 40.14

Gene sequence:

>1602_bases
ATGATGATGAATGTACCATTAACAATTCATTCGATGATGGAAAGGGCAGAAACATTATTTCCAAAGAAAGAAATTATTTC
TCGTACACATGATAGGGTTACCACGTTAACGTATAAACAAATTGGAGAACGAACGCGAAGGCTTTCTAGTGTATTAAAGA
AATTGGGAGTAAACGAAGGAGAACGAATTGGAACGTTGGCATGGAATCATCATCGACATGTGGAAGCATACTTTGCTATT
CCTGGTATTGGTTCTGTATTGCACACAATTAATATTCGTTTATCTCCCCAGCATATTTCTTATATAGTCAACCATGCCGA
AGATCGAATTTTACTTATTGATGAAGATTTGGTCCCGCTTGTGGAAAAGATACAAGGAGAATTATCGACTGTACAAGCAT
ATATCATTATGACGGATAAAGAGGAAATACCAGAGACTTCATTGACGCCTGTATATCATTATGAGCACCTTCTCGCTGAA
GGAAATCCGAAGCATCAATTTTTAAAAGAAATTGATGAAAATATGCCGGCTGGTATGTGCTATACTTCGGCAACAACAGG
AAATCCAAAAGGTGTTGTATATACGCATCGTAGTACAGTGCTTCATTGTATGGCGCTTGGATTAGCTGATACAGCAGCGC
TATCGGAAAGCGATGTGGCAATGGCGATTGTGCCGATGTTTCATGTGAATGCATGGGGATTACCATTTGCGGCAACGTGG
TTTGGAACGAAGCAAGTTTTGCCTGGTCCGATGTTTACACCGAAAATTTTACTGGAAATGATTCAAAAAGAGAAAGTGAC
AGTAGCAGCTGGAGTACCTACAATTTGGCTTGGTGTTTTGCAAGAATTAGAGCAACATGACTATGATTTATCGAGTATGA
GCCGGATTTTATGCGGGGGAGCTGCGGCACCCAAAAGTGTAATCGCAGCATTCGAACAGAAATATCACGTTCCTTTTGTA
CACGCGTATGGTATGACAGAAACGAGCCCACTTGTAACACTTGCAAGGTTAAAAAGTTATGAAAAAGATTTATCATATGA
AGAGCAACTTGAAATAAGATCAAAACAAGGATATCTTGTGCCGGGAGTTGAAATGAAAATAGTTGGAGGAAATGGAGAAG
TGAAATGGGACGGGAGTGAGATGGGAGAACTTTGCTTACGTGCACCGTGGATTGCTGCAAGTTACTATAAAGATGAGCGT
ACAGCGGAAGGATTTCGTGATGGGTGGTTATATACGGGGGATGTTGTAACGGTAGATGAAGAAGGATGCGTGAAAATAGT
AGATCGAACAAAAGATGTGATTAAAAGCGGGGGTGAATGGATCTCATCTGTAGATTTAGAGAATGCACTTATGGCGCATG
AAGCGGTGTTTGAAGCAGCAGTTGTTGCAGTTCCCCATCCTAAGTGGCAAGAGCGACCTGTTGCTTGTGTCGTACGAAAA
CCAAATCGTGTCGTAACGAAAGAAGAGTTATATGAATTTTTACAACCGCAATTTGCGAAGTGGTGGTTACCCGATGATAT
TGTATTTATGAAAGAAATCCCAAAAACATCAGTAGGGAAATTTTTAAAACAAGCATTGCGAAAAGAATTGAACAAGAAAT
AA

Upstream 100 bases:

>100_bases
GGGAATAATTTCAGAAAATTTAGCAAAAAACAACTTTTATTTTGTTGAATTTGATATAATGATCAAAAATGGAAGCGTTT
TAATGGAGGGTGAGATACGT

Downstream 100 bases:

>100_bases
ATGTATGTTATCGGTACTTGAACTGGGACTTCGATGTGAGATACCGAGTATGACAATTTATTTAAAAAACAGAATACTAG
CAAAAAATCCATAAACAGAA

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MMMNVPLTIHSMMERAETLFPKKEIISRTHDRVTTLTYKQIGERTRRLSSVLKKLGVNEGERIGTLAWNHHRHVEAYFAI
PGIGSVLHTINIRLSPQHISYIVNHAEDRILLIDEDLVPLVEKIQGELSTVQAYIIMTDKEEIPETSLTPVYHYEHLLAE
GNPKHQFLKEIDENMPAGMCYTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDVAMAIVPMFHVNAWGLPFAATW
FGTKQVLPGPMFTPKILLEMIQKEKVTVAAGVPTIWLGVLQELEQHDYDLSSMSRILCGGAAAPKSVIAAFEQKYHVPFV
HAYGMTETSPLVTLARLKSYEKDLSYEEQLEIRSKQGYLVPGVEMKIVGGNGEVKWDGSEMGELCLRAPWIAASYYKDER
TAEGFRDGWLYTGDVVTVDEEGCVKIVDRTKDVIKSGGEWISSVDLENALMAHEAVFEAAVVAVPHPKWQERPVACVVRK
PNRVVTKEELYEFLQPQFAKWWLPDDIVFMKEIPKTSVGKFLKQALRKELNKK

Sequences:

>Translated_533_residues
MMMNVPLTIHSMMERAETLFPKKEIISRTHDRVTTLTYKQIGERTRRLSSVLKKLGVNEGERIGTLAWNHHRHVEAYFAI
PGIGSVLHTINIRLSPQHISYIVNHAEDRILLIDEDLVPLVEKIQGELSTVQAYIIMTDKEEIPETSLTPVYHYEHLLAE
GNPKHQFLKEIDENMPAGMCYTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDVAMAIVPMFHVNAWGLPFAATW
FGTKQVLPGPMFTPKILLEMIQKEKVTVAAGVPTIWLGVLQELEQHDYDLSSMSRILCGGAAAPKSVIAAFEQKYHVPFV
HAYGMTETSPLVTLARLKSYEKDLSYEEQLEIRSKQGYLVPGVEMKIVGGNGEVKWDGSEMGELCLRAPWIAASYYKDER
TAEGFRDGWLYTGDVVTVDEEGCVKIVDRTKDVIKSGGEWISSVDLENALMAHEAVFEAAVVAVPHPKWQERPVACVVRK
PNRVVTKEELYEFLQPQFAKWWLPDDIVFMKEIPKTSVGKFLKQALRKELNKK
>Mature_533_residues
MMMNVPLTIHSMMERAETLFPKKEIISRTHDRVTTLTYKQIGERTRRLSSVLKKLGVNEGERIGTLAWNHHRHVEAYFAI
PGIGSVLHTINIRLSPQHISYIVNHAEDRILLIDEDLVPLVEKIQGELSTVQAYIIMTDKEEIPETSLTPVYHYEHLLAE
GNPKHQFLKEIDENMPAGMCYTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDVAMAIVPMFHVNAWGLPFAATW
FGTKQVLPGPMFTPKILLEMIQKEKVTVAAGVPTIWLGVLQELEQHDYDLSSMSRILCGGAAAPKSVIAAFEQKYHVPFV
HAYGMTETSPLVTLARLKSYEKDLSYEEQLEIRSKQGYLVPGVEMKIVGGNGEVKWDGSEMGELCLRAPWIAASYYKDER
TAEGFRDGWLYTGDVVTVDEEGCVKIVDRTKDVIKSGGEWISSVDLENALMAHEAVFEAAVVAVPHPKWQERPVACVVRK
PNRVVTKEELYEFLQPQFAKWWLPDDIVFMKEIPKTSVGKFLKQALRKELNKK

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=555, Percent_Identity=27.2072072072072, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI187761345, Length=546, Percent_Identity=26.7399267399267, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI187761343, Length=546, Percent_Identity=26.7399267399267, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI38505220, Length=508, Percent_Identity=26.1811023622047, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI42544132, Length=513, Percent_Identity=24.7563352826511, Blast_Score=119, Evalue=9e-27,
Organism=Homo sapiens, GI27477105, Length=527, Percent_Identity=23.719165085389, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI28416953, Length=533, Percent_Identity=24.953095684803, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI157311624, Length=515, Percent_Identity=21.747572815534, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI157311622, Length=515, Percent_Identity=21.747572815534, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI122937307, Length=516, Percent_Identity=24.031007751938, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI58082049, Length=521, Percent_Identity=22.2648752399232, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI115511026, Length=513, Percent_Identity=22.2222222222222, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI13375727, Length=549, Percent_Identity=22.040072859745, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI83745141, Length=515, Percent_Identity=22.7184466019417, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1788107, Length=525, Percent_Identity=26.0952380952381, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI145693145, Length=366, Percent_Identity=32.2404371584699, Blast_Score=162, Evalue=7e-41,
Organism=Escherichia coli, GI221142682, Length=505, Percent_Identity=23.1683168316832, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1786810, Length=370, Percent_Identity=30, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789201, Length=506, Percent_Identity=23.9130434782609, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1790505, Length=532, Percent_Identity=21.9924812030075, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788595, Length=500, Percent_Identity=21.8, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17531443, Length=510, Percent_Identity=25.6862745098039, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17559526, Length=366, Percent_Identity=31.6939890710383, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17558820, Length=511, Percent_Identity=27.0058708414873, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI71994694, Length=564, Percent_Identity=26.5957446808511, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI71994690, Length=564, Percent_Identity=26.5957446808511, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI71994703, Length=564, Percent_Identity=26.5957446808511, Blast_Score=148, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17560308, Length=569, Percent_Identity=25.6590509666081, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17538037, Length=512, Percent_Identity=24.0234375, Blast_Score=136, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=510, Percent_Identity=24.1176470588235, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI32563687, Length=507, Percent_Identity=25.4437869822485, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17560140, Length=352, Percent_Identity=26.7045454545455, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71996755, Length=515, Percent_Identity=24.8543689320388, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71985884, Length=372, Percent_Identity=23.1182795698925, Blast_Score=106, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319699, Length=359, Percent_Identity=27.0194986072423, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI21355181, Length=496, Percent_Identity=27.0161290322581, Blast_Score=171, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24581924, Length=559, Percent_Identity=27.0125223613596, Blast_Score=148, Evalue=8e-36,
Organism=Drosophila melanogaster, GI18859661, Length=408, Percent_Identity=27.2058823529412, Blast_Score=136, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24648253, Length=501, Percent_Identity=22.7544910179641, Blast_Score=112, Evalue=9e-25,
Organism=Drosophila melanogaster, GI21356441, Length=502, Percent_Identity=22.5099601593625, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648255, Length=501, Percent_Identity=22.9540918163673, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281365686, Length=482, Percent_Identity=20.746887966805, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648260, Length=498, Percent_Identity=21.4859437751004, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24656500, Length=516, Percent_Identity=21.5116279069767, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922652, Length=520, Percent_Identity=20.5769230769231, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21358303, Length=496, Percent_Identity=22.3790322580645, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24648257, Length=538, Percent_Identity=23.2342007434944, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24653035, Length=504, Percent_Identity=21.6269841269841, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI161076582, Length=518, Percent_Identity=22.3938223938224, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20130357, Length=527, Percent_Identity=22.011385199241, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21356947, Length=508, Percent_Identity=21.6535433070866, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 59999; Mature: 59999

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMNVPLTIHSMMERAETLFPKKEIISRTHDRVTTLTYKQIGERTRRLSSVLKKLGVNEG
CEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ERIGTLAWNHHRHVEAYFAIPGIGSVLHTINIRLSPQHISYIVNHAEDRILLIDEDLVPL
CEEEEEEECCCCEEEEEEECCCHHHHHHEEEEEECHHHHHHHHHCCCCEEEEECCCHHHH
VEKIQGELSTVQAYIIMTDKEEIPETSLTPVYHYEHLLAEGNPKHQFLKEIDENMPAGMC
HHHHCCCCEEEEEEEEEECHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCEE
YTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDVAMAIVPMFHVNAWGLPFAATW
EECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCCEEEEC
FGTKQVLPGPMFTPKILLEMIQKEKVTVAAGVPTIWLGVLQELEQHDYDLSSMSRILCGG
CCCHHCCCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHCC
AAAPKSVIAAFEQKYHVPFVHAYGMTETSPLVTLARLKSYEKDLSYEEQLEIRSKQGYLV
CCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEE
PGVEMKIVGGNGEVKWDGSEMGELCLRAPWIAASYYKDERTAEGFRDGWLYTGDVVTVDE
CCEEEEEECCCCEEEECCHHHHHHHHHCCCHHHHHHCCHHHHCHHHCCCEEECCEEEECC
EGCVKIVDRTKDVIKSGGEWISSVDLENALMAHEAVFEAAVVAVPHPKWQERPVACVVRK
CCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEC
PNRVVTKEELYEFLQPQFAKWWLPDDIVFMKEIPKTSVGKFLKQALRKELNKK
CCCCCCHHHHHHHHCCHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMMNVPLTIHSMMERAETLFPKKEIISRTHDRVTTLTYKQIGERTRRLSSVLKKLGVNEG
CEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ERIGTLAWNHHRHVEAYFAIPGIGSVLHTINIRLSPQHISYIVNHAEDRILLIDEDLVPL
CEEEEEEECCCCEEEEEEECCCHHHHHHEEEEEECHHHHHHHHHCCCCEEEEECCCHHHH
VEKIQGELSTVQAYIIMTDKEEIPETSLTPVYHYEHLLAEGNPKHQFLKEIDENMPAGMC
HHHHCCCCEEEEEEEEEECHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCEE
YTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDVAMAIVPMFHVNAWGLPFAATW
EECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCCEEEEC
FGTKQVLPGPMFTPKILLEMIQKEKVTVAAGVPTIWLGVLQELEQHDYDLSSMSRILCGG
CCCHHCCCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHCC
AAAPKSVIAAFEQKYHVPFVHAYGMTETSPLVTLARLKSYEKDLSYEEQLEIRSKQGYLV
CCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEE
PGVEMKIVGGNGEVKWDGSEMGELCLRAPWIAASYYKDERTAEGFRDGWLYTGDVVTVDE
CCEEEEEECCCCEEEECCHHHHHHHHHCCCHHHHHHCCHHHHCHHHCCCEEECCEEEECC
EGCVKIVDRTKDVIKSGGEWISSVDLENALMAHEAVFEAAVVAVPHPKWQERPVACVVRK
CCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEC
PNRVVTKEELYEFLQPQFAKWWLPDDIVFMKEIPKTSVGKFLKQALRKELNKK
CCCCCCHHHHHHHHCCHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]