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The map label for this gene is argH2 [H]

Identifier: 152975952

GI number: 152975952

Start: 2319483

End: 2320991

Strand: Reverse

Name: argH2 [H]

Synonym: Bcer98_2224

Alternate gene names: 152975952

Gene position: 2320991-2319483 (Counterclockwise)

Preceding gene: 152975953

Following gene: 152975950

Centisome position: 56.79

GC content: 35.26

Gene sequence:

>1509_bases
ATGAAGCAAATGAAAGAAGAATTTATAGAGGAAGAAGGCAGTGATTTCCCTGGAAAGACCTATATGAATTGTGTGTTGAA
ACATGTGTTTGAGTTTCAAAGAGAGCATTTGTTATATGATATGTTTATGGTTCACAGAGCTCATATCATTATGTTAATAG
AAGAAAAAATAATAAGTTTACAAGAGGGAAAAGAGATTTTAACAGCAGTCGAGAAAGTCGCACAAATTCCGAAAGACGAG
CTGCATTATAATTCGAAATATGAAGATCTTTTCTTTTTGATTGAACATTTCATCATAAAAGAAGCAAATGACAATACGAT
TAGTAATATGCATATTGGCAGAAGCCGGAATGATATGGGAGTGACAATGTATCGGTTGAGTTTGCGTCGTTATCTATTAC
GTTTTATGGAACATTTTATGTTATTACAGGAAAGTATATTACAAGTTGCTCATAATCATATCGATACAATTATGCCTGCT
TACACACATACACAACCAGCTCAACCAACAACATTTGGTCATTACATGGTAGCTGTTTATGATGTAATGGGGCGAGACTT
GGAGAGGATTTGGAAAACATATCATTTCTTGAATAATTCACCAATGGGAGCTGCGGCTCTTTCTACAACGAGTTTTCTGA
TTAATAGGAAAAGAGTAGCTGAATTACTTGGTTTTTCAAGTGTTATTGAAAACTCTTATGATGCAGTTGCAGGTGCTGAT
TATTTGTTAGAAGTGAGTTCTTTACTCATGGTGACAATGACGAATATGGGGAGATGGATACATGATTTTTTACTGCTTGC
AACGAAAGAATATAATGGTATCACGGTTGCGAATCCATATGTGCAAATTAGTAGCATCATGCCGCAAAAACGTAATCCGG
TATCGATTGAACACGCTCGTGCCCTCACTAGTAGTGCGATAGGAGAAGCTTTTACTGTCTTTCAAATGATCCACAATACT
CCGTTTGGTGATATTGTAGATACAGAGGATGATTTACAACCGTATTTATATGAGGGAATGGAAAAAGCAATTCGTGTATT
TTGTACGATGAATGCAGTAGTTCGAACAATGAAAGTAGAAGAGAAAGTTTTAAAAGACCGATCATTTCAGCACGCTATTA
CCATTACGGATTTGGCAGACACATTAACAAAGAATTATCATATTCCTTTTCGTCTTGCTCATCATGCTGCAAGTGAAGTT
GCAAAAATGTCTTTGGAACAAAGCAAAGAATTGCATGAATTAGAGCTAGGAGAAATAAACGTATACTTACAAAAGCGATT
TAAAATAAAGCTTTCAAAGGAAGAATGGGAAAATAGTATATTGCCAGAAATGTTTGTAAAGAAACGAATTGTATATGGAG
GACCAAGTCACCAAGAGATGGAGCGGATGATTTTAAAAAGAAGAGCAGCATTTGAGCAAGCAGAGAAGAATTTTGAAAGA
GAAAAACAGCGCATTTCTAAAGTAGAAAAAGAAACAATGCAACTTGTTACAGACATGATCAGATTGTAA

Upstream 100 bases:

>100_bases
AAACCGGGTGCAATATGCACCTGGTTTATTTTTGTACAAGCACCTTTTTATATTTATTGGGTATGATGGATAGTAATAGT
TCTTAACGAGGGGGGAGAGA

Downstream 100 bases:

>100_bases
AATGATATTTTTATATATAGACTTAACAATAAATTAAGGCATTGAATATGAACGAATCATGGAATCCATATGACTATTTT
TAGAATCGATTAAGTGAGAA

Product: argininosuccinate lyase

Products: NA

Alternate protein names: ASAL 2; Arginosuccinase [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MKQMKEEFIEEEGSDFPGKTYMNCVLKHVFEFQREHLLYDMFMVHRAHIIMLIEEKIISLQEGKEILTAVEKVAQIPKDE
LHYNSKYEDLFFLIEHFIIKEANDNTISNMHIGRSRNDMGVTMYRLSLRRYLLRFMEHFMLLQESILQVAHNHIDTIMPA
YTHTQPAQPTTFGHYMVAVYDVMGRDLERIWKTYHFLNNSPMGAAALSTTSFLINRKRVAELLGFSSVIENSYDAVAGAD
YLLEVSSLLMVTMTNMGRWIHDFLLLATKEYNGITVANPYVQISSIMPQKRNPVSIEHARALTSSAIGEAFTVFQMIHNT
PFGDIVDTEDDLQPYLYEGMEKAIRVFCTMNAVVRTMKVEEKVLKDRSFQHAITITDLADTLTKNYHIPFRLAHHAASEV
AKMSLEQSKELHELELGEINVYLQKRFKIKLSKEEWENSILPEMFVKKRIVYGGPSHQEMERMILKRRAAFEQAEKNFER
EKQRISKVEKETMQLVTDMIRL

Sequences:

>Translated_502_residues
MKQMKEEFIEEEGSDFPGKTYMNCVLKHVFEFQREHLLYDMFMVHRAHIIMLIEEKIISLQEGKEILTAVEKVAQIPKDE
LHYNSKYEDLFFLIEHFIIKEANDNTISNMHIGRSRNDMGVTMYRLSLRRYLLRFMEHFMLLQESILQVAHNHIDTIMPA
YTHTQPAQPTTFGHYMVAVYDVMGRDLERIWKTYHFLNNSPMGAAALSTTSFLINRKRVAELLGFSSVIENSYDAVAGAD
YLLEVSSLLMVTMTNMGRWIHDFLLLATKEYNGITVANPYVQISSIMPQKRNPVSIEHARALTSSAIGEAFTVFQMIHNT
PFGDIVDTEDDLQPYLYEGMEKAIRVFCTMNAVVRTMKVEEKVLKDRSFQHAITITDLADTLTKNYHIPFRLAHHAASEV
AKMSLEQSKELHELELGEINVYLQKRFKIKLSKEEWENSILPEMFVKKRIVYGGPSHQEMERMILKRRAAFEQAEKNFER
EKQRISKVEKETMQLVTDMIRL
>Mature_502_residues
MKQMKEEFIEEEGSDFPGKTYMNCVLKHVFEFQREHLLYDMFMVHRAHIIMLIEEKIISLQEGKEILTAVEKVAQIPKDE
LHYNSKYEDLFFLIEHFIIKEANDNTISNMHIGRSRNDMGVTMYRLSLRRYLLRFMEHFMLLQESILQVAHNHIDTIMPA
YTHTQPAQPTTFGHYMVAVYDVMGRDLERIWKTYHFLNNSPMGAAALSTTSFLINRKRVAELLGFSSVIENSYDAVAGAD
YLLEVSSLLMVTMTNMGRWIHDFLLLATKEYNGITVANPYVQISSIMPQKRNPVSIEHARALTSSAIGEAFTVFQMIHNT
PFGDIVDTEDDLQPYLYEGMEKAIRVFCTMNAVVRTMKVEEKVLKDRSFQHAITITDLADTLTKNYHIPFRLAHHAASEV
AKMSLEQSKELHELELGEINVYLQKRFKIKLSKEEWENSILPEMFVKKRIVYGGPSHQEMERMILKRRAAFEQAEKNFER
EKQRISKVEKETMQLVTDMIRL

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI31541964, Length=377, Percent_Identity=26.790450928382, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI68303542, Length=377, Percent_Identity=26.790450928382, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI68303547, Length=377, Percent_Identity=26.525198938992, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI68303549, Length=377, Percent_Identity=25.1989389920424, Blast_Score=119, Evalue=7e-27,
Organism=Escherichia coli, GI1790398, Length=426, Percent_Identity=28.169014084507, Blast_Score=176, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6321806, Length=374, Percent_Identity=28.6096256684492, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI221473854, Length=313, Percent_Identity=29.073482428115, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI78706858, Length=313, Percent_Identity=29.073482428115, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF00206 Lyase_1 [H]

EC number: =4.3.2.1 [H]

Molecular weight: Translated: 58543; Mature: 58543

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]