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The map label for this gene is nprE [H]

Identifier: 152975941

GI number: 152975941

Start: 2298356

End: 2300026

Strand: Reverse

Name: nprE [H]

Synonym: Bcer98_2213

Alternate gene names: 152975941

Gene position: 2300026-2298356 (Counterclockwise)

Preceding gene: 152975943

Following gene: 152975939

Centisome position: 56.28

GC content: 34.29

Gene sequence:

>1671_bases
ATGAAGAAAACGGTTGTTACATTGCTGACTGCTGGTGCAGTTTTAGGTGCACCATTTTCAACTGCTTTTGCAGAAGAACA
AGCGTCACAACAAAAAATTATGGATCAAATGGAAGTCGTACAAAAAGATTGGAATGACGAAAAAGGGAACCCGTCTTTCC
TAGCGGGAGAATTATCAACTAAAAATGTACAGACTCAAAAGGAAGTCGAGAAATTTTTGGAAGATAATAAAGCTTTATTT
AAACTTGACCCAAAAACAGACTTGACACTTAAAGAAGTGAAATCAGATGATTTGGGAATGAAACACTATGTTTACACTCA
ATCTATCAATAAGGTACCAGTTGATGGTGCAAGATTTATGATTCATACAAATAAAGAGGGTAAAGTAACAACAGTAAATG
GAAATATACACCCGGATGCTGCGGAGAATGTAAGAAAGAATACAACTGCTAAAATTTCGAAAGAAGCAGCTCTTTCAAAT
GCGTGGAAACATATTAATCTTACAAAAAATGACACGTTAGTCGAAGGGAATGGAAATGTATTAGATAAAGTAAAAGACAA
CTTAGAATCTACAAGTGAAAAAGCTGATTTAGTCGTATATGAAAAAGAAGGAAAATATTATTTAACTTTTAAAGTACAAT
TGCAATTTGTTAAACCTTATGGAGCTAATTGGCAAATTTATGTTAATGCAGAAGATGGAACAATTGTTGATTCATATAAT
GCAGTTACAGATGCAGATATTGCTAAAAAAGGCTATGGTTATGGTGTATTAGGTGACCGAAAAGAACTAAACACTACTTA
TAATAGTACAAAGGGAAAATATTATTTACGTGATATGACAAAGCCTATGAATGGAGGAGTCATTGAAACATTTACAGTCA
ACCACAGTGATGCAGATTATCCGGTTAACTACTACCTATTAGATGATGATAATGCATGGATCAATAAAAATCAAAGACCT
GCGGTTGATGCTCATTATCATGCAGGAAAAGTCTATGATTATTATAAAAATATTCATAACCGAAACAGTATTGATGGAAA
AGGTAAAACGATTCGTTCAGCTGTCAATTATGGAACTAATGTCAATAATGCATTTTGGAATGGGCAGCAAATGATTTATG
GAGATGGAGACGGTTATGAATTTATTCCACTTTCAGGTTCACTTGATGTTGTTGCCCATGAATTAACACATGCAGTAACT
CAATATTCAGCTGATTTGCAATATGTGAATCAATCTGGTGCATTAAATGAATCTTTCTCTGATGTATTTGGATATTTTGT
CGATCCAAACAACTGGGATTTAGGGGAAGCTGTATATACACCTAGAGTTGCTGGAGATGCACTTCGTAGTTTATCGGATC
CAGAAAAATATGGACAGCCTGCTCATATGAAAGATTATCAATTTTTACCTCCTACTGAAGAAGGAGATAATGGGGGCGTG
CATATTAACAGCGGGATTCCAAATAAGGCGGCTTATTTAACAATTAACGCTATTGGTAAAGAAAAAGCGGAAAAAATCTA
TTACCGTGCTTTAACAACTTATTTAACACCAACAAGTAACTTTAAACAAGCTCGTGCAGCTTTATTACAATCTGCAGCTG
ATTATGATGGTTATGATAGCGCAACATATAAGGCAGTAGAAAATGCTTGGAATCAAGTTGGTATAAATTAA

Upstream 100 bases:

>100_bases
GCAATTATTCATATTTGTATGCTTTGTCTAAAAAATAATTGTATATTAAGTTATGCAATTTTATTTTGATATATTACGAA
TATTGAAAGGAGAAACAAAG

Downstream 100 bases:

>100_bases
TTTATTATTCTATTCAACTAAGATATTTTATAAAATGCAATAGTAAAAATCAAAAAGGAATCCCTCACGGATTCCTTTTT
CTGATTAAGTTTTTAGAAAG

Product: peptidase M4 thermolysin

Products: NA

Alternate protein names: MCP 76; Neutral protease [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MKKTVVTLLTAGAVLGAPFSTAFAEEQASQQKIMDQMEVVQKDWNDEKGNPSFLAGELSTKNVQTQKEVEKFLEDNKALF
KLDPKTDLTLKEVKSDDLGMKHYVYTQSINKVPVDGARFMIHTNKEGKVTTVNGNIHPDAAENVRKNTTAKISKEAALSN
AWKHINLTKNDTLVEGNGNVLDKVKDNLESTSEKADLVVYEKEGKYYLTFKVQLQFVKPYGANWQIYVNAEDGTIVDSYN
AVTDADIAKKGYGYGVLGDRKELNTTYNSTKGKYYLRDMTKPMNGGVIETFTVNHSDADYPVNYYLLDDDNAWINKNQRP
AVDAHYHAGKVYDYYKNIHNRNSIDGKGKTIRSAVNYGTNVNNAFWNGQQMIYGDGDGYEFIPLSGSLDVVAHELTHAVT
QYSADLQYVNQSGALNESFSDVFGYFVDPNNWDLGEAVYTPRVAGDALRSLSDPEKYGQPAHMKDYQFLPPTEEGDNGGV
HINSGIPNKAAYLTINAIGKEKAEKIYYRALTTYLTPTSNFKQARAALLQSAADYDGYDSATYKAVENAWNQVGIN

Sequences:

>Translated_556_residues
MKKTVVTLLTAGAVLGAPFSTAFAEEQASQQKIMDQMEVVQKDWNDEKGNPSFLAGELSTKNVQTQKEVEKFLEDNKALF
KLDPKTDLTLKEVKSDDLGMKHYVYTQSINKVPVDGARFMIHTNKEGKVTTVNGNIHPDAAENVRKNTTAKISKEAALSN
AWKHINLTKNDTLVEGNGNVLDKVKDNLESTSEKADLVVYEKEGKYYLTFKVQLQFVKPYGANWQIYVNAEDGTIVDSYN
AVTDADIAKKGYGYGVLGDRKELNTTYNSTKGKYYLRDMTKPMNGGVIETFTVNHSDADYPVNYYLLDDDNAWINKNQRP
AVDAHYHAGKVYDYYKNIHNRNSIDGKGKTIRSAVNYGTNVNNAFWNGQQMIYGDGDGYEFIPLSGSLDVVAHELTHAVT
QYSADLQYVNQSGALNESFSDVFGYFVDPNNWDLGEAVYTPRVAGDALRSLSDPEKYGQPAHMKDYQFLPPTEEGDNGGV
HINSGIPNKAAYLTINAIGKEKAEKIYYRALTTYLTPTSNFKQARAALLQSAADYDGYDSATYKAVENAWNQVGIN
>Mature_556_residues
MKKTVVTLLTAGAVLGAPFSTAFAEEQASQQKIMDQMEVVQKDWNDEKGNPSFLAGELSTKNVQTQKEVEKFLEDNKALF
KLDPKTDLTLKEVKSDDLGMKHYVYTQSINKVPVDGARFMIHTNKEGKVTTVNGNIHPDAAENVRKNTTAKISKEAALSN
AWKHINLTKNDTLVEGNGNVLDKVKDNLESTSEKADLVVYEKEGKYYLTFKVQLQFVKPYGANWQIYVNAEDGTIVDSYN
AVTDADIAKKGYGYGVLGDRKELNTTYNSTKGKYYLRDMTKPMNGGVIETFTVNHSDADYPVNYYLLDDDNAWINKNQRP
AVDAHYHAGKVYDYYKNIHNRNSIDGKGKTIRSAVNYGTNVNNAFWNGQQMIYGDGDGYEFIPLSGSLDVVAHELTHAVT
QYSADLQYVNQSGALNESFSDVFGYFVDPNNWDLGEAVYTPRVAGDALRSLSDPEKYGQPAHMKDYQFLPPTEEGDNGGV
HINSGIPNKAAYLTINAIGKEKAEKIYYRALTTYLTPTSNFKQARAALLQSAADYDGYDSATYKAVENAWNQVGIN

Specific function: Extracellular zinc metalloprotease [H]

COG id: COG3227

COG function: function code E; Zinc metalloprotease (elastase)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005075
- InterPro:   IPR013856
- InterPro:   IPR001570
- InterPro:   IPR011096 [H]

Pfam domain/function: PF07504 FTP; PF03413 PepSY; PF01447 Peptidase_M4; PF02868 Peptidase_M4_C [H]

EC number: =3.4.24.28 [H]

Molecular weight: Translated: 61952; Mature: 61952

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTVVTLLTAGAVLGAPFSTAFAEEQASQQKIMDQMEVVQKDWNDEKGNPSFLAGELST
CCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCC
KNVQTQKEVEKFLEDNKALFKLDPKTDLTLKEVKSDDLGMKHYVYTQSINKVPVDGARFM
CCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHCCCCCCCCEEEEEECCCCCCCCCCEEEE
IHTNKEGKVTTVNGNIHPDAAENVRKNTTAKISKEAALSNAWKHINLTKNDTLVEGNGNV
EEECCCCEEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHCHHEEEECCCCEEEECCCCC
LDKVKDNLESTSEKADLVVYEKEGKYYLTFKVQLQFVKPYGANWQIYVNAEDGTIVDSYN
HHHHHHHHHHCCCCCEEEEEEECCCEEEEEEEEEEEECCCCCCEEEEEECCCCEEEECCC
AVTDADIAKKGYGYGVLGDRKELNTTYNSTKGKYYLRDMTKPMNGGVIETFTVNHSDADY
CCCCCHHHHCCCCCCCCCCCHHCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCC
PVNYYLLDDDNAWINKNQRPAVDAHYHAGKVYDYYKNIHNRNSIDGKGKTIRSAVNYGTN
CEEEEEECCCCCEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCC
VNNAFWNGQQMIYGDGDGYEFIPLSGSLDVVAHELTHAVTQYSADLQYVNQSGALNESFS
CCCCEECCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHH
DVFGYFVDPNNWDLGEAVYTPRVAGDALRSLSDPEKYGQPAHMKDYQFLPPTEEGDNGGV
HHCEEEECCCCCCCCCHHCCCCHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCE
HINSGIPNKAAYLTINAIGKEKAEKIYYRALTTYLTPTSNFKQARAALLQSAADYDGYDS
EECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
ATYKAVENAWNQVGIN
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKTVVTLLTAGAVLGAPFSTAFAEEQASQQKIMDQMEVVQKDWNDEKGNPSFLAGELST
CCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCC
KNVQTQKEVEKFLEDNKALFKLDPKTDLTLKEVKSDDLGMKHYVYTQSINKVPVDGARFM
CCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHCCCCCCCCEEEEEECCCCCCCCCCEEEE
IHTNKEGKVTTVNGNIHPDAAENVRKNTTAKISKEAALSNAWKHINLTKNDTLVEGNGNV
EEECCCCEEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHCHHEEEECCCCEEEECCCCC
LDKVKDNLESTSEKADLVVYEKEGKYYLTFKVQLQFVKPYGANWQIYVNAEDGTIVDSYN
HHHHHHHHHHCCCCCEEEEEEECCCEEEEEEEEEEEECCCCCCEEEEEECCCCEEEECCC
AVTDADIAKKGYGYGVLGDRKELNTTYNSTKGKYYLRDMTKPMNGGVIETFTVNHSDADY
CCCCCHHHHCCCCCCCCCCCHHCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCC
PVNYYLLDDDNAWINKNQRPAVDAHYHAGKVYDYYKNIHNRNSIDGKGKTIRSAVNYGTN
CEEEEEECCCCCEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCC
VNNAFWNGQQMIYGDGDGYEFIPLSGSLDVVAHELTHAVTQYSADLQYVNQSGALNESFS
CCCCEECCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHH
DVFGYFVDPNNWDLGEAVYTPRVAGDALRSLSDPEKYGQPAHMKDYQFLPPTEEGDNGGV
HHCEEEECCCCCCCCCHHCCCCHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCE
HINSGIPNKAAYLTINAIGKEKAEKIYYRALTTYLTPTSNFKQARAALLQSAADYDGYDS
EECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
ATYKAVENAWNQVGIN
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA