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The map label for this gene is 152975789

Identifier: 152975789

GI number: 152975789

Start: 2143100

End: 2144140

Strand: Reverse

Name: 152975789

Synonym: Bcer98_2039

Alternate gene names: NA

Gene position: 2144140-2143100 (Counterclockwise)

Preceding gene: 152975790

Following gene: 152975788

Centisome position: 52.46

GC content: 39.67

Gene sequence:

>1041_bases
ATGAAGGTGCTTATATTAGGAGGTACGCGCTTTTTAGGAAGAGCATTAGTAGAAGAAGCTTTGAAACGAGGACATGAAGT
AACTTTGTTTAATCGCGGTACAAATAAAGAAATATTTCCTAAAGTAGAACAGCTAATTGGCGATAGAGATGGAGATGTGT
CTGCTTTAAAGAATCGAAATTGGGATATTGTGATGGATACATGTGGATTTGCACCACATCAAATTCGTAACATTGCCACC
ATACTTGGAGGAAATATCGAACATTATACATTTATTTCAAGCATGTCTACCTATAAAGATTGGATTCCACTGCATATTAA
GGAAGACTATCAATTACAGTCTATTCCACCTAGGGAGAAGTTGAAAGCGATTGAGGACGGGGAGGCATCTCCTTACGAGT
ATTACGGAGGATTAAAAGTGTTATGTGAGAAGGAAGCAGAAAAATATTGGCCAGGGCGAGTTTTGCATATGAGAGCAGGA
CTTTTAGTTGGGGCATTTGATTACACAGACCGGCTTCCGTACTGGATTCAGCGTGTAGCACAAGGGGGGGAAGTATTGGT
TCCAGAGCATTCCAACCGTCCTGTTCAATTGATTGACGTAAAAGATGTGGCAACGTGGGCGTTTAATATGGCAGAAGGCA
GAAAAGTAGGCACGTTTAATGTAACAGGACCGAATAATGAATTGACGATAGGAGAGTTATTAAATACTTGCAAAGCTGTT
ACCAACAGCGATGCTGAATTTGTATGGGTGGAAGAACAATTTTTACTAGAAAATAAAGTGCAGCCATGGACGGAGATGCC
TCTATGGATTCCTGAAACTTTTCCGTTAGAAGGAGAAACAGAACCGTGGAAGGGGAGTTTTTTTATAAGTATTGAAAAAG
CTGTTAACGCTGGACTTTCTTTTAGACCCATTCAAAATACGATTTCTGATGTGTATCAATGGGAAAAATCGAGGAAAGAT
ACAGAACGAAAAGCAGGTATGGCAAGAGAAAAAGAGAGGGAATTGTTAAAGCTTTGGTTTCAACAAGAGAAAATGCGATG
A

Upstream 100 bases:

>100_bases
AGCGACAAATAATTTCGTTATTCTCCCGTAACTTTTTAACTAGGAAAATCGTTATATAAATTGAATGCTAATTTTTTAGT
TTGTGAAATGGGGGAAAGAT

Downstream 100 bases:

>100_bases
GAAGATAAGGTTAGAATGCAGAGGTGGATTGTGTTTAGGAATTTTATATAAATGATTGAAATATAAGGTATATCCCCCGC
CTTCATTGAAGGCAGGGGGG

Product: NAD-dependent epimerase/dehydratase

Products: 2'-hydroxyformononetin; NADPH; H+

Alternate protein names: Reductase; NAD Dependent Epimerase/Dehydratase Family Protein; Isoflavone Reductase; NAD Dependent Epimerase/Dehydratase; Nucleoside-Diphosphate-Sugar Epimerase; NAD-Dependent Epimerase/Dehydratase Family Protein; Nucleotide Sugar Epimerase; 3-Beta Hydroxysteroid Dehydrogenase/Isomerase; 3-Beta Hydroxysteroid Dehydrogenase/Isomerase Family; Oxidoreductase; DTDP-Glucose 4 6-Dehydratase; NmrA Family Protein; Sugar Dehydratase; Dtdpglucose 4 6-Dehydratase-Like Protein; NAD-Dependent Protein; NAD Dependent Epimerase; NAD Dependent Epimerase/Dehydratase Family

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKVLILGGTRFLGRALVEEALKRGHEVTLFNRGTNKEIFPKVEQLIGDRDGDVSALKNRNWDIVMDTCGFAPHQIRNIAT
ILGGNIEHYTFISSMSTYKDWIPLHIKEDYQLQSIPPREKLKAIEDGEASPYEYYGGLKVLCEKEAEKYWPGRVLHMRAG
LLVGAFDYTDRLPYWIQRVAQGGEVLVPEHSNRPVQLIDVKDVATWAFNMAEGRKVGTFNVTGPNNELTIGELLNTCKAV
TNSDAEFVWVEEQFLLENKVQPWTEMPLWIPETFPLEGETEPWKGSFFISIEKAVNAGLSFRPIQNTISDVYQWEKSRKD
TERKAGMAREKERELLKLWFQQEKMR

Sequences:

>Translated_346_residues
MKVLILGGTRFLGRALVEEALKRGHEVTLFNRGTNKEIFPKVEQLIGDRDGDVSALKNRNWDIVMDTCGFAPHQIRNIAT
ILGGNIEHYTFISSMSTYKDWIPLHIKEDYQLQSIPPREKLKAIEDGEASPYEYYGGLKVLCEKEAEKYWPGRVLHMRAG
LLVGAFDYTDRLPYWIQRVAQGGEVLVPEHSNRPVQLIDVKDVATWAFNMAEGRKVGTFNVTGPNNELTIGELLNTCKAV
TNSDAEFVWVEEQFLLENKVQPWTEMPLWIPETFPLEGETEPWKGSFFISIEKAVNAGLSFRPIQNTISDVYQWEKSRKD
TERKAGMAREKERELLKLWFQQEKMR
>Mature_346_residues
MKVLILGGTRFLGRALVEEALKRGHEVTLFNRGTNKEIFPKVEQLIGDRDGDVSALKNRNWDIVMDTCGFAPHQIRNIAT
ILGGNIEHYTFISSMSTYKDWIPLHIKEDYQLQSIPPREKLKAIEDGEASPYEYYGGLKVLCEKEAEKYWPGRVLHMRAG
LLVGAFDYTDRLPYWIQRVAQGGEVLVPEHSNRPVQLIDVKDVATWAFNMAEGRKVGTFNVTGPNNELTIGELLNTCKAV
TNSDAEFVWVEEQFLLENKVQPWTEMPLWIPETFPLEGETEPWKGSFFISIEKAVNAGLSFRPIQNTISDVYQWEKSRKD
TERKAGMAREKERELLKLWFQQEKMR

Specific function: Unknown

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.3.1.45

Molecular weight: Translated: 39798; Mature: 39798

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLILGGTRFLGRALVEEALKRGHEVTLFNRGTNKEIFPKVEQLIGDRDGDVSALKNRN
CEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHCCC
WDIVMDTCGFAPHQIRNIATILGGNIEHYTFISSMSTYKDWIPLHIKEDYQLQSIPPREK
CEEEEECCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCEEEEEECCCEECCCCCHHH
LKAIEDGEASPYEYYGGLKVLCEKEAEKYWPGRVLHMRAGLLVGAFDYTDRLPYWIQRVA
HHHHCCCCCCCHHHHCCCCEEEEHHHHHCCCCEEEEEECCEEEEECCCCCCCHHHHHHHH
QGGEVLVPEHSNRPVQLIDVKDVATWAFNMAEGRKVGTFNVTGPNNELTIGELLNTCKAV
CCCCEEECCCCCCCEEEEEECHHHHHHHHHCCCCEEEEEEECCCCCCEEHHHHHHHHHHH
TNSDAEFVWVEEQFLLENKVQPWTEMPLWIPETFPLEGETEPWKGSFFISIEKAVNAGLS
CCCCCCEEEEEHHHHHHCCCCCHHCCCEECCCCCCCCCCCCCCCCEEEEEEEHHHCCCCC
FRPIQNTISDVYQWEKSRKDTERKAGMAREKERELLKLWFQQEKMR
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVLILGGTRFLGRALVEEALKRGHEVTLFNRGTNKEIFPKVEQLIGDRDGDVSALKNRN
CEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHCCC
WDIVMDTCGFAPHQIRNIATILGGNIEHYTFISSMSTYKDWIPLHIKEDYQLQSIPPREK
CEEEEECCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCEEEEEECCCEECCCCCHHH
LKAIEDGEASPYEYYGGLKVLCEKEAEKYWPGRVLHMRAGLLVGAFDYTDRLPYWIQRVA
HHHHCCCCCCCHHHHCCCCEEEEHHHHHCCCCEEEEEECCEEEEECCCCCCCHHHHHHHH
QGGEVLVPEHSNRPVQLIDVKDVATWAFNMAEGRKVGTFNVTGPNNELTIGELLNTCKAV
CCCCEEECCCCCCCEEEEEECHHHHHHHHHCCCCEEEEEEECCCCCCEEHHHHHHHHHHH
TNSDAEFVWVEEQFLLENKVQPWTEMPLWIPETFPLEGETEPWKGSFFISIEKAVNAGLS
CCCCCCEEEEEHHHHHHCCCCCHHCCCEECCCCCCCCCCCCCCCCEEEEEEEHHHCCCCC
FRPIQNTISDVYQWEKSRKDTERKAGMAREKERELLKLWFQQEKMR
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vestitone; NADP+

Specific reaction: vestitone + NADP+ = 2'-hydroxyformononetin + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA