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The map label for this gene is rihA [H]

Identifier: 152975735

GI number: 152975735

Start: 2084045

End: 2085001

Strand: Reverse

Name: rihA [H]

Synonym: Bcer98_1982

Alternate gene names: 152975735

Gene position: 2085001-2084045 (Counterclockwise)

Preceding gene: 152975736

Following gene: 152975734

Centisome position: 51.02

GC content: 36.15

Gene sequence:

>957_bases
GTGAATAAGAAAATCATCTTTTTTGGAGACTTTGGTATTGATGATGCAGTATCGTTAATCTATGCAGATAAGACGTGTCG
TCTTGATATCATTGGAATTGTTGCTGATTACGGAAATGTTTCAAGGGAAATTGTGACTGAAAATGTTTATTTTTTAGAGA
GATACTATGCTACAAAGATAAAAATTATAAAAGGAGCCAGTAGGCCAATGACGGCAGAAGATCCTTTGTTCTTTCCAGAA
ATTCATGGCGAACACGGTTTGGGATCAATTATTCCTCCAAAAACAAGAGGGATGAAAAGAGAAAACTTTTATGAGATTAT
CAAGTTAATCGAACCGTGTCCAGAAGATATTATCATTGTAGCAACAGGACGTTTAACAGCTCTCGCAACATTATTTCTGT
TGTATCCTGAAGTAATGAGTTGCGTGCATGCGTATTATATTATGGGTGGTGCGTTTTTATGTCCCGGTAATGTTACACCT
GTATCAGAAGCGAATTTTTATAGTGATCCCATCGCAACAAACATTGTGATGAAGTACGCGAAAAACGTAAGTATTTACCC
ATTAAATGTGACGCAGCGGGCCATTATTACTCCTGAAATGGTAAATTTTATTGACGATAAGGGAACAGGACAAGCAAAGC
TGATTAAACCAATGATTGATTTTTACTATGAAAATTATTATAAAAAGCAATACCCTGGAATCGGTGGCAGTCCAATTCAT
GATTTACTTCCATTTATTGCATTGCGAAAAGATACTATTTTTGAATACAAAAAGTCTGCTGTCTGGATTAGTACAACAAA
TGATGAGACGCGTGGACAAAGTGTAGCAGACTTTAGAAGGACAGCGGAGCAAACGAGATTTGATAATCGACCAGTTCAGA
GGATTGCGGTGAACTTTCGTTATCAAGCATTTAAAAGAGAATTTATGAGTACAATGTTAAAACCAGATTGTTCTTGA

Upstream 100 bases:

>100_bases
TCACTAATAAATAGGTGACTTTTTTGCTTCTCATGGTTTCACCGCCTTGTCTTTTTTGCATAATAATGGTATTAGAATGT
GCAGAAGAGGGGTGAGGATA

Downstream 100 bases:

>100_bases
CTAGCGTTACTCCCAAATCATATATGGTCATTTTATTTTCAACGTTCGTATATAATGAACTTCTTTAGTACCTAGCGAAC
GAGCATATCCTATAACCTTC

Product: inosine/uridine-preferring nucleoside hydrolase

Products: D-ribose; purine

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MNKKIIFFGDFGIDDAVSLIYADKTCRLDIIGIVADYGNVSREIVTENVYFLERYYATKIKIIKGASRPMTAEDPLFFPE
IHGEHGLGSIIPPKTRGMKRENFYEIIKLIEPCPEDIIIVATGRLTALATLFLLYPEVMSCVHAYYIMGGAFLCPGNVTP
VSEANFYSDPIATNIVMKYAKNVSIYPLNVTQRAIITPEMVNFIDDKGTGQAKLIKPMIDFYYENYYKKQYPGIGGSPIH
DLLPFIALRKDTIFEYKKSAVWISTTNDETRGQSVADFRRTAEQTRFDNRPVQRIAVNFRYQAFKREFMSTMLKPDCS

Sequences:

>Translated_318_residues
MNKKIIFFGDFGIDDAVSLIYADKTCRLDIIGIVADYGNVSREIVTENVYFLERYYATKIKIIKGASRPMTAEDPLFFPE
IHGEHGLGSIIPPKTRGMKRENFYEIIKLIEPCPEDIIIVATGRLTALATLFLLYPEVMSCVHAYYIMGGAFLCPGNVTP
VSEANFYSDPIATNIVMKYAKNVSIYPLNVTQRAIITPEMVNFIDDKGTGQAKLIKPMIDFYYENYYKKQYPGIGGSPIH
DLLPFIALRKDTIFEYKKSAVWISTTNDETRGQSVADFRRTAEQTRFDNRPVQRIAVNFRYQAFKREFMSTMLKPDCS
>Mature_318_residues
MNKKIIFFGDFGIDDAVSLIYADKTCRLDIIGIVADYGNVSREIVTENVYFLERYYATKIKIIKGASRPMTAEDPLFFPE
IHGEHGLGSIIPPKTRGMKRENFYEIIKLIEPCPEDIIIVATGRLTALATLFLLYPEVMSCVHAYYIMGGAFLCPGNVTP
VSEANFYSDPIATNIVMKYAKNVSIYPLNVTQRAIITPEMVNFIDDKGTGQAKLIKPMIDFYYENYYKKQYPGIGGSPIH
DLLPFIALRKDTIFEYKKSAVWISTTNDETRGQSVADFRRTAEQTRFDNRPVQRIAVNFRYQAFKREFMSTMLKPDCS

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=307, Percent_Identity=30.2931596091205, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788486, Length=245, Percent_Identity=26.9387755102041, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786213, Length=280, Percent_Identity=27.8571428571429, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320608, Length=252, Percent_Identity=30.1587301587302, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 36230; Mature: 36230

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKIIFFGDFGIDDAVSLIYADKTCRLDIIGIVADYGNVSREIVTENVYFLERYYATKI
CCCCEEEEECCCCCHHHHEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEE
KIIKGASRPMTAEDPLFFPEIHGEHGLGSIIPPKTRGMKRENFYEIIKLIEPCPEDIIIV
EEEECCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
ATGRLTALATLFLLYPEVMSCVHAYYIMGGAFLCPGNVTPVSEANFYSDPIATNIVMKYA
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHH
KNVSIYPLNVTQRAIITPEMVNFIDDKGTGQAKLIKPMIDFYYENYYKKQYPGIGGSPIH
CCCEEEEECCCCCEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
DLLPFIALRKDTIFEYKKSAVWISTTNDETRGQSVADFRRTAEQTRFDNRPVQRIAVNFR
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
YQAFKREFMSTMLKPDCS
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNKKIIFFGDFGIDDAVSLIYADKTCRLDIIGIVADYGNVSREIVTENVYFLERYYATKI
CCCCEEEEECCCCCHHHHEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEE
KIIKGASRPMTAEDPLFFPEIHGEHGLGSIIPPKTRGMKRENFYEIIKLIEPCPEDIIIV
EEEECCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
ATGRLTALATLFLLYPEVMSCVHAYYIMGGAFLCPGNVTPVSEANFYSDPIATNIVMKYA
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHH
KNVSIYPLNVTQRAIITPEMVNFIDDKGTGQAKLIKPMIDFYYENYYKKQYPGIGGSPIH
CCCEEEEECCCCCEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
DLLPFIALRKDTIFEYKKSAVWISTTNDETRGQSVADFRRTAEQTRFDNRPVQRIAVNFR
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
YQAFKREFMSTMLKPDCS
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA