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The map label for this gene is proP [H]

Identifier: 152975248

GI number: 152975248

Start: 1555833

End: 1557281

Strand: Direct

Name: proP [H]

Synonym: Bcer98_1453

Alternate gene names: 152975248

Gene position: 1555833-1557281 (Clockwise)

Preceding gene: 152975247

Following gene: 152975249

Centisome position: 38.07

GC content: 34.78

Gene sequence:

>1449_bases
ATGCAACAAAGACATAATTTAGATTTTGGACCTCAAGATGTTAATATTATTAATCCTAAACAAGCAAAAAAAGCTGTTGT
AGCTACTGGCATTGGGAATATGATGGAGTGGTTTGATTTTGGTTTATATGCTTATTTAGCGGTTATTTTAAGTCAATTAT
TCTTTTCAAACGTCCATAATAGTAGTCTCCAACTTGTACTTACATTTGGTACATTTGCTGCTGCCTTTCTCGTTCGCCCA
ATTGGAGGCATTTTCTTTGGGAGAATTGGCGATAAATATGGGCGGAAGGTTGTTTTAAGCACGACGATTATTTTAATGGC
GCTTTCTACATTATTCATTGCTCTACTGCCAACTTATGAACAAATTGGGATTTGGGCACCGATATTCTTATTAGTTGCTC
GGATCGTTCAAGGGTTCTCTACAGGAGGCGAATATTCGGGTGCAATGGTTTATATCGCAGAATCTTCTCCAGATAAAAAG
CGTGGAATACTTGGTAGTGGTCTTGAAATTGGAACGCTTTCAGGTTACATTGCCGCTTCAGGAATCGTTACCATTTTGAC
AGTATTATTAACAAATGAACAAATGCTTAGTTGGGGATGGCGTATTCCTTTCTTAATTGCTGCACCAATTGGTTTGGTCG
GTTTATATTTACGCCGTCATTTGAGTGAATCTCCAGTCTTTCAAGAGATGGAAAAAGAAAAAGCACAAGAGGAATATAAA
GATGACAAACAAATTCCTTTGATTTACATTCTAAAATATTATAAAAAAGATTTTCTTCTAAGTATAGTCATTGTCGCCTT
CTTTAACATTACTAACTATATGATCCTTTCTTTTATTCCTTCTTATCTAACGCAAATAGTAAAAATGAAAGAAACAACTG
GTTTAATAATCATTTCAATTACGATGACACTTATGATTCCACTGGCATTTTATTTTGGTAAATTAAGTGATAAGGTTGGA
AATAAACGTGTCGTACAATTTGGCTTATTGGGTTTAACATTATGTTCAATTCCAGCATTTTTACTAATAAGACATGGACA
TATTGTAGCTATTTTCGCTGGTATTTTTGTTTTAGGCTTTTTCTTAAGTATTTATGAAGGAACACTACCTTCCTTATTAC
CATCTCTCTTTTTCACAGATGTGCGTTATCGTGCACTGTCTATATCTTTCAATATTTCTGTATCCATATTCGGTGGCACA
ACGCCACTTGTATCTTCATATCTTGTTCACATTACTGATAATGCACTTGCACCAGCGTTTTATTTAACAGGTGTAAGCTT
AATTGGTTTAATTGTATTTAGTCTATTATTTGTGACAACATCAGGACGTGCATTGAAAGGTGCTTATCCTACAGTTGAAA
CAAAAAAAGAAGCTTATGAAGTAGCTAAGCAAGTCCCTGAAGAAGCACTTTGGTGGCATGAAGAAGCTTTAGAAGTGAAA
AAGTCATAG

Upstream 100 bases:

>100_bases
AAGGAAGGGTATTTTTCCTTAAGTGCTTCGTATTGAAATTATTCTATAAGAGAAGGTGAGCTTGATGAATTTAAATTAAA
TAAGGAGCTGTTTTTATTTT

Downstream 100 bases:

>100_bases
TTTACGAAAAAAACGATATCATTTTTTGGGAATTTCAAAATATAAAAGATCCCTATCTTATTTAAAGAGGATAGAAGTCT
TTTATATAGTTAGGTATTTT

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MQQRHNLDFGPQDVNIINPKQAKKAVVATGIGNMMEWFDFGLYAYLAVILSQLFFSNVHNSSLQLVLTFGTFAAAFLVRP
IGGIFFGRIGDKYGRKVVLSTTIILMALSTLFIALLPTYEQIGIWAPIFLLVARIVQGFSTGGEYSGAMVYIAESSPDKK
RGILGSGLEIGTLSGYIAASGIVTILTVLLTNEQMLSWGWRIPFLIAAPIGLVGLYLRRHLSESPVFQEMEKEKAQEEYK
DDKQIPLIYILKYYKKDFLLSIVIVAFFNITNYMILSFIPSYLTQIVKMKETTGLIIISITMTLMIPLAFYFGKLSDKVG
NKRVVQFGLLGLTLCSIPAFLLIRHGHIVAIFAGIFVLGFFLSIYEGTLPSLLPSLFFTDVRYRALSISFNISVSIFGGT
TPLVSSYLVHITDNALAPAFYLTGVSLIGLIVFSLLFVTTSGRALKGAYPTVETKKEAYEVAKQVPEEALWWHEEALEVK
KS

Sequences:

>Translated_482_residues
MQQRHNLDFGPQDVNIINPKQAKKAVVATGIGNMMEWFDFGLYAYLAVILSQLFFSNVHNSSLQLVLTFGTFAAAFLVRP
IGGIFFGRIGDKYGRKVVLSTTIILMALSTLFIALLPTYEQIGIWAPIFLLVARIVQGFSTGGEYSGAMVYIAESSPDKK
RGILGSGLEIGTLSGYIAASGIVTILTVLLTNEQMLSWGWRIPFLIAAPIGLVGLYLRRHLSESPVFQEMEKEKAQEEYK
DDKQIPLIYILKYYKKDFLLSIVIVAFFNITNYMILSFIPSYLTQIVKMKETTGLIIISITMTLMIPLAFYFGKLSDKVG
NKRVVQFGLLGLTLCSIPAFLLIRHGHIVAIFAGIFVLGFFLSIYEGTLPSLLPSLFFTDVRYRALSISFNISVSIFGGT
TPLVSSYLVHITDNALAPAFYLTGVSLIGLIVFSLLFVTTSGRALKGAYPTVETKKEAYEVAKQVPEEALWWHEEALEVK
KS
>Mature_482_residues
MQQRHNLDFGPQDVNIINPKQAKKAVVATGIGNMMEWFDFGLYAYLAVILSQLFFSNVHNSSLQLVLTFGTFAAAFLVRP
IGGIFFGRIGDKYGRKVVLSTTIILMALSTLFIALLPTYEQIGIWAPIFLLVARIVQGFSTGGEYSGAMVYIAESSPDKK
RGILGSGLEIGTLSGYIAASGIVTILTVLLTNEQMLSWGWRIPFLIAAPIGLVGLYLRRHLSESPVFQEMEKEKAQEEYK
DDKQIPLIYILKYYKKDFLLSIVIVAFFNITNYMILSFIPSYLTQIVKMKETTGLIIISITMTLMIPLAFYFGKLSDKVG
NKRVVQFGLLGLTLCSIPAFLLIRHGHIVAIFAGIFVLGFFLSIYEGTLPSLLPSLFFTDVRYRALSISFNISVSIFGGT
TPLVSSYLVHITDNALAPAFYLTGVSLIGLIVFSLLFVTTSGRALKGAYPTVETKKEAYEVAKQVPEEALWWHEEALEVK
KS

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=453, Percent_Identity=41.9426048565121, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1788292, Length=423, Percent_Identity=30.9692671394799, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1789941, Length=405, Percent_Identity=34.0740740740741, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1788942, Length=425, Percent_Identity=29.6470588235294, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI87082404, Length=444, Percent_Identity=26.1261261261261, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87082231, Length=451, Percent_Identity=24.6119733924612, Blast_Score=93, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53472; Mature: 53472

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CC
>Mature Secondary Structure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CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]