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The map label for this gene is fliF [H]

Identifier: 152975158

GI number: 152975158

Start: 1458990

End: 1460555

Strand: Direct

Name: fliF [H]

Synonym: Bcer98_1359

Alternate gene names: 152975158

Gene position: 1458990-1460555 (Clockwise)

Preceding gene: 152975157

Following gene: 152975159

Centisome position: 35.7

GC content: 35.95

Gene sequence:

>1566_bases
ATGGAAAAGATAAAAAATGTTTTCCAATCGCTAAAAACATGGCATAAGATGGTTATTGGTGCTGCGATTTTAGCGATTGG
AACAGGAGCGCTTTTATATTTCACCTTGCCAGATAAATATGTTGTTGTCTATCAAAATTTAAATGATGCAGATAAGCAAG
AAATTACAGCAGAATTATCAAAGCTAGGTGTAGATTATCAGTTAGTTAATGATGGAGCTATTAAAGTTCCAACAAAAGAT
GCTCCTTGGATTCGAAAAGAAATGAATGGAATGGGATTGCCGTTTAATTCAAAAAGCGGTGAAGAGATTTTATTAGAAAG
TTCTCTTGGATCTAGTGAACAAGACAAGAAGATGAAACAGCTTGTCGGTACGAAGAAACAACTTGAACAAGATATTGTAA
GAAACTTTGCAACAATTGAAAGTGCTAATGTTCAAATTACGTTACCTGAAAAAGAAACGATTTTTGACGAAGAAAAGGCG
AAAGGAACGGCTGCGATTACAGTTGGGGTAAAACGTGGTCAGCTATTAACTGAAGATCAAGTTGCAGGTATTCAGCAAAT
GATTAGCGCAGCAGTTTCTGGAGTAAAGCCAGAGGAAGTTAGCGTTATTGATAGTAAAAAAGGTATTGTTTCAAAAGGTG
CGGATGCATCAACACATGCAAGTTCGTCTTCTTATGAAAAAGAAGTAGAAATGCAACAGCAAATTGAAAACAAGCTGAAA
CAAGATATTGATGCGACATTAATGACAATGTTTAAAGCGAATGAGTTTAAGGTTAATACAAAAGTTAGTGTTAATTATGA
TGAAGTGACACGTCAATCCGAGAAATATGGTGATAAGGGCGTTCTTCGTAGTAGACAAGACCAACAGGAACGTTCAACTG
CGCAAGAAGGCGCTGACGCAAAACAAGGCGCAGGTATTACGGCAAATGGCGAAGTTCCAAATTATGGTACGAATAATAAT
CAAAATGGTAAAGTTGTTTACGACAATAATAATGGTAATAAAATTGAAAACTATGAAATTGATAAGACAGTTGAGACGAT
TAAAAAACATCCAGAATTAACAAAAACAAATGTTGTTGTTTGGGTAGATAACGATACATTAACAAAACGAAGAATTGATA
TGACGACATTTAAGGAAGCAATTGGAACAGCAGCTGGTTTACAAATTGATCCAAATGGAGAATTTACAAATGGCCAAGTG
AATGTTGTTACTGTTCAATTTGATCAACAAAAAGAAGAGAAAACGAAAGAACCAGAACCGAATGGCTTCAACTGGTGGTT
AATCGGAGGAATTGCGGGAGGACTTCTTGCATTAGGTGGTTTGGTTTGGTATTTATTAGCAAGACGTAAGAGAAAACAAG
AAGAAATAGAGGAATATGAATCATTGGCAGAGGAAGAAGTAGCAGCTACTGATGAAACAATTATGGAAATTCCTGAAGAA
AAACTAGAACCAACAGAACAGGCAGAACCAACAGAACCAACGTTAGATGAACAAGTGCAAGATGCTGCAAAAGAGCATGT
AGAGGGCACTGCAAAAGTTATTAAAAAATGGTTAAATGGACAGTAA

Upstream 100 bases:

>100_bases
TCACAAATGAAAACAGCTGCTGTTATACGTGACAATCTGATTGAAAACTACAAGCAGTTACTTAATATGCAAATTTAGGA
AAATGGACGGGTGTGTTTAA

Downstream 100 bases:

>100_bases
GGGAGGAAAAATGATGCTAGATGAAATCTCCTCCAAAGAAAAAGCTGCGATTCTTATTCGGACATTGGATGAAAAAGTAG
CAGCGAAAGTCATTGAATAT

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MEKIKNVFQSLKTWHKMVIGAAILAIGTGALLYFTLPDKYVVVYQNLNDADKQEITAELSKLGVDYQLVNDGAIKVPTKD
APWIRKEMNGMGLPFNSKSGEEILLESSLGSSEQDKKMKQLVGTKKQLEQDIVRNFATIESANVQITLPEKETIFDEEKA
KGTAAITVGVKRGQLLTEDQVAGIQQMISAAVSGVKPEEVSVIDSKKGIVSKGADASTHASSSSYEKEVEMQQQIENKLK
QDIDATLMTMFKANEFKVNTKVSVNYDEVTRQSEKYGDKGVLRSRQDQQERSTAQEGADAKQGAGITANGEVPNYGTNNN
QNGKVVYDNNNGNKIENYEIDKTVETIKKHPELTKTNVVVWVDNDTLTKRRIDMTTFKEAIGTAAGLQIDPNGEFTNGQV
NVVTVQFDQQKEEKTKEPEPNGFNWWLIGGIAGGLLALGGLVWYLLARRKRKQEEIEEYESLAEEEVAATDETIMEIPEE
KLEPTEQAEPTEPTLDEQVQDAAKEHVEGTAKVIKKWLNGQ

Sequences:

>Translated_521_residues
MEKIKNVFQSLKTWHKMVIGAAILAIGTGALLYFTLPDKYVVVYQNLNDADKQEITAELSKLGVDYQLVNDGAIKVPTKD
APWIRKEMNGMGLPFNSKSGEEILLESSLGSSEQDKKMKQLVGTKKQLEQDIVRNFATIESANVQITLPEKETIFDEEKA
KGTAAITVGVKRGQLLTEDQVAGIQQMISAAVSGVKPEEVSVIDSKKGIVSKGADASTHASSSSYEKEVEMQQQIENKLK
QDIDATLMTMFKANEFKVNTKVSVNYDEVTRQSEKYGDKGVLRSRQDQQERSTAQEGADAKQGAGITANGEVPNYGTNNN
QNGKVVYDNNNGNKIENYEIDKTVETIKKHPELTKTNVVVWVDNDTLTKRRIDMTTFKEAIGTAAGLQIDPNGEFTNGQV
NVVTVQFDQQKEEKTKEPEPNGFNWWLIGGIAGGLLALGGLVWYLLARRKRKQEEIEEYESLAEEEVAATDETIMEIPEE
KLEPTEQAEPTEPTLDEQVQDAAKEHVEGTAKVIKKWLNGQ
>Mature_521_residues
MEKIKNVFQSLKTWHKMVIGAAILAIGTGALLYFTLPDKYVVVYQNLNDADKQEITAELSKLGVDYQLVNDGAIKVPTKD
APWIRKEMNGMGLPFNSKSGEEILLESSLGSSEQDKKMKQLVGTKKQLEQDIVRNFATIESANVQITLPEKETIFDEEKA
KGTAAITVGVKRGQLLTEDQVAGIQQMISAAVSGVKPEEVSVIDSKKGIVSKGADASTHASSSSYEKEVEMQQQIENKLK
QDIDATLMTMFKANEFKVNTKVSVNYDEVTRQSEKYGDKGVLRSRQDQQERSTAQEGADAKQGAGITANGEVPNYGTNNN
QNGKVVYDNNNGNKIENYEIDKTVETIKKHPELTKTNVVVWVDNDTLTKRRIDMTTFKEAIGTAAGLQIDPNGEFTNGQV
NVVTVQFDQQKEEKTKEPEPNGFNWWLIGGIAGGLLALGGLVWYLLARRKRKQEEIEEYESLAEEEVAATDETIMEIPEE
KLEPTEQAEPTEPTLDEQVQDAAKEHVEGTAKVIKKWLNGQ

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=374, Percent_Identity=24.8663101604278, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 57780; Mature: 57780

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIKNVFQSLKTWHKMVIGAAILAIGTGALLYFTLPDKYVVVYQNLNDADKQEITAELS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHH
KLGVDYQLVNDGAIKVPTKDAPWIRKEMNGMGLPFNSKSGEEILLESSLGSSEQDKKMKQ
HCCCCEEEECCCEEECCCCCCCHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHH
LVGTKKQLEQDIVRNFATIESANVQITLPEKETIFDEEKAKGTAAITVGVKRGQLLTEDQ
HHCCHHHHHHHHHHHHHHEECCCEEEEECCCHHHCCHHHCCCCEEEEEEECCCCCCCHHH
VAGIQQMISAAVSGVKPEEVSVIDSKKGIVSKGADASTHASSSSYEKEVEMQQQIENKLK
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QDIDATLMTMFKANEFKVNTKVSVNYDEVTRQSEKYGDKGVLRSRQDQQERSTAQEGADA
HHHHHHHHHHHCCCCEEEEEEEEECHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHCCCCH
KQGAGITANGEVPNYGTNNNQNGKVVYDNNNGNKIENYEIDKTVETIKKHPELTKTNVVV
HHCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCEEHHHHHHHHHHCCCCCCCEEEE
WVDNDTLTKRRIDMTTFKEAIGTAAGLQIDPNGEFTNGQVNVVTVQFDQQKEEKTKEPEP
EECCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCEEEEEEEEECCCHHHHCCCCCC
NGFNWWLIGGIAGGLLALGGLVWYLLARRKRKQEEIEEYESLAEEEVAATDETIMEIPEE
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
KLEPTEQAEPTEPTLDEQVQDAAKEHVEGTAKVIKKWLNGQ
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKIKNVFQSLKTWHKMVIGAAILAIGTGALLYFTLPDKYVVVYQNLNDADKQEITAELS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHH
KLGVDYQLVNDGAIKVPTKDAPWIRKEMNGMGLPFNSKSGEEILLESSLGSSEQDKKMKQ
HCCCCEEEECCCEEECCCCCCCHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHH
LVGTKKQLEQDIVRNFATIESANVQITLPEKETIFDEEKAKGTAAITVGVKRGQLLTEDQ
HHCCHHHHHHHHHHHHHHEECCCEEEEECCCHHHCCHHHCCCCEEEEEEECCCCCCCHHH
VAGIQQMISAAVSGVKPEEVSVIDSKKGIVSKGADASTHASSSSYEKEVEMQQQIENKLK
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QDIDATLMTMFKANEFKVNTKVSVNYDEVTRQSEKYGDKGVLRSRQDQQERSTAQEGADA
HHHHHHHHHHHCCCCEEEEEEEEECHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHCCCCH
KQGAGITANGEVPNYGTNNNQNGKVVYDNNNGNKIENYEIDKTVETIKKHPELTKTNVVV
HHCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCEEHHHHHHHHHHCCCCCCCEEEE
WVDNDTLTKRRIDMTTFKEAIGTAAGLQIDPNGEFTNGQVNVVTVQFDQQKEEKTKEPEP
EECCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCEEEEEEEEECCCHHHHCCCCCC
NGFNWWLIGGIAGGLLALGGLVWYLLARRKRKQEEIEEYESLAEEEVAATDETIMEIPEE
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
KLEPTEQAEPTEPTLDEQVQDAAKEHVEGTAKVIKKWLNGQ
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]